Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002851

Physical Location & Seq

Genomic Coordinates Forward (+)

13945743 .. 13951125

Gene Structure 5383 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGCTTGCTGGAGTGAGGATCATACCCCGAGACCAGATTGATAAGGGTGAGCATCTGAGTGCTCCTGGGAAAGAAAAACAAAAACAGAAGAAGAGGTCGGGTAATAAGGAGAATAAGTGCAGGAAGGAAAAGAAGCGCTCTCACTCTAGTAGTTCGGATGGGGAAGAGCTTGATAGGATGAAGAAGGGGTCTAAAAAAAAGTGGTATTCATCAGACGAGTATTCATCATCATCATCATATTCGTCAGGCGTTGAAAGTGAGGGAAGTTCCGACCGAGATAGAAGGAGGAGTCAGAGTAGGAAAAAAGGTAAGAGAAGTACTGAGGATGATTCAGAGGATGAGGCCAGTTATGGGCCAAAAAGGAGATTAAGGAGGGGAAGGAAAGAATATGTATCAGAAGATCATTATTCTTCTGCTTCTGATGGTAAAGACAGTGATAATTCTGATAGAAAAGAAAGGTGCAAAAACGGAAGTAAAAAAGACAGAAAGAAAAAGAGTAAGCGAAGGAGTCTAGGAGACATGGGGGATGAAGAATTTACTGATGGAGATAGAGAACCCAATCGTTTGAAAGATGGGGAGATTGCAAGGAAGGAATTTGGCTTAGAGTGGATGCTTAGACCTGAAGGAAAAACAGATAGAACCCCTACAGTGAATGCTGAGGAGCAGCCAAAGGAAACTCTGACTGAAGAGATAAAGGTCAATCCCAGGGAATTAAATCCATATTTAAAGGATGACGGATCTGGTTATCCAGAAGATACAGATGAACCTAAAGCTGCTGGAAACCAACTTTTGTCTTCCTCAGTTGTTGGGGATGGAGGTGCAAGTTGGAAACTAAAAGCTCTGAAGCGTGCAAAAGAGCAGGCAACTCGAGAAGGACGAAAGCTCCAGGAGGTTGTCGGAGAGCGTTGGGGTTCTCTTGGTCAACTGACCGTCTCCATGGCATCTAATAAAGCTGCCCAATCTCGTGCTCATCTACAGGCTATAAGAAGTAGACAGAAAGGGCCGAGTGAGGAACGCCAACCAGATTCCAGCAATCAAAGTGACAAGGATGATAAAAAGGATTCTAGTCTTTTAGCGGATGCAGTAGCTAGCTTAAATAAGTTTTCAAACGATGGAAGCTTCATGTCTGAAGTTCTTCGTAAAAGGACTGATGATTTTAGTAGTCATGCTCATGAAAATATAGCAGCCACTAGCAAACCAAGTGAAGCCACTAGCAAACCAAGTGAAGCGAGTATTGCATTTCATGAACTAAGTGCGAACCAGTTGGCAGCTAAGGCTTTTCAACTTCGTATGAAAGGAAAGCTAGAAGAAGCTGAGGAACTTCTGCAAGAAGTAGAAAACAGAAAGGCAAAGCAGGCTAGTGGAGATAATTCTGTGAGACCAGGAAATGAACAGAGCACAAGCAGACATGTTATGCAGGAAATGAATCTTCGTCAGAAGAAAAAGGAGGATGATGCTGATATGCATCTAGCTCAAAGGATTACCCAGAACAAAAAATACAGTATATCTGGTCAGGCAGATGATGAATATGACTATGATGATGCTCCAAGAAAGAAGTCCAAGAAAAAGCAGGGTAGTGATCAGAAAGTCACCCAGAACAACAGTTTTGCAACTCGATTTGCAACGCAGCAGGAGAGATGTTTCTTTTGTTTTGAAAATCCGAGGAGGCCGGCGCATCTTGTTGTGGCCATAGCAAACTTCACATATTTAATGTTGCCACAGCAGCAGCCTGTTGTGCCTGGTCACTGTTGTATTTTACCAATGCAGCATGTGGCTTCCACAAGAACTGTTGACGACGATGTGTGGCAAGAAATTCGAAATTTCAAGAAATGCCTTATTATGATGTTTGCAAAGCAAGAGAAGGAAGTAGTTTTTCTTGAAACTGTGATGGGGTTGGCTCAACAACGCCGCCACTGTATAGTTGAGTGTATTCCTATGCCCCATGAAATTGCCAAAGAGGCTCCTCTATATTTCAAAAAGGCAATTGATGAAGCTGAAGAAGAGTGGAGCCAGCACAATGCAAAGAGACTAATTGATACAAGTGTGAAGGGCTTGCGTGGGTCGATTCCAAAGGACTTTCCGTACTTCCATGTGGAGTTTGGATTGAATAAAGGGTTTGTCCACGTTATTGATGACGAACAGCAGTTCAACAGCAGTCTTGGACTGAATGTCATTAGAGGCATGCTACAATTGCCTGAGGAAGACATGTATCGTCGCCGAAGACACGAGACGGTGGAGGCACAAAAGCAAGCGGTTAAGAGCTTCGATCAAGATTGGGCAGCTTTTGACTGGACAAAACAGCTTCACCAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2

Protein Analysis

772

Amino Acids

87.8

Weight (kDa)

8.94

Isoelectric Point (pI)

58.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CwfJ_C_1	PF04677	539 - 659	1.1e-42	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	668 - 765	2.9e-28	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56290
fragaria_vesca	FvH4_4g02820 FvH4_4g02820 FvH4_4g02820 FvH4_4g02820
malus_domestica	MD13G1208100.v1.1 MD16G1209600.v1.1
prunus_persica	Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1 Prupe.1G031700_v2.0.a1
pyrus_communis	pycom13g18020 pycom16g17690
rosa_chinensis	RchiOBHm_Chr4g0390791 RchiOBHm_Chr5g0000461
rosa_laevigata	RLG00000009911 RLG00000030869
rosa_multiflora	Rmu_sc0000171.1_g000026 Rmu_sc0001380.1_g000027 Rmu_sc0003949.1_g000006
rosa_roxburghii	Rroxscaffold_1G00075700 Rroxscaffold_5G00336660 Rroxscaffold_5G00336680 Rroxscaffold_5G00336690
rosa_rugosa	Rorug03G0331500 Rorug03G0331500 Rorug04G0383800
rosa_samantha	Rh4AG033200 Rh4BG027000 Rh4CG036800 Rh4DG030000 Rh5AG003800 Rh5BG005000 Rh5CG004100 Rh5DG004000
rosa_wichuraiana	Rw5G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	991
AciI	CCGC	3 cut(s)	1076, 1909, 2252
AclWI	GGATC	2 cut(s)	26, 745
AcoI	YGGCCR	1 cut(s)	1686
AcsI	RAATTY	4 cut(s)	533, 593, 1812, 1819
AcuI	CTGAAG	5 cut(s)	642, 705, 863, 1149, 2016
AfaI	GTAC	2 cut(s)	319, 2084
AfeI	AGCGCT	1 cut(s)	137
AfiI	CCNNNNNNNGG	1 cut(s)	351
AflIII	ACRYGT	2 cut(s)	1408, 2205
AhdI	GACNNNNNGTC	1 cut(s)	2168
AjnI	CCWGG	5 cut(s)	64, 704, 885, 1381, 1738
Alw21I	GWGCWC	3 cut(s)	64, 970, 1400
Alw26I	GTCTC	6 cut(s)	24, 510, 937, 1372, 2020, 2222
AlwI	GGATC	2 cut(s)	26, 745
Ama87I	CYCGRG	2 cut(s)	27, 867
Aor51HI	AGCGCT	1 cut(s)	137
AoxI	GGCC	5 cut(s)	342, 353, 1001, 1667, 1686
ApoI	RAATTY	4 cut(s)	533, 593, 1812, 1819
Asp700I	GAANNNNTTC	1 cut(s)	1134
AspLEI	GCGC	2 cut(s)	138, 1675
AspS9I	GGNCC	2 cut(s)	353, 1001
AsuHPI	GGTGA	3 cut(s)	59, 1582, 2297
AsuII	TTCGAA	1 cut(s)	1816
AsuNHI	GCTAGC	1 cut(s)	1088
AvaI	CYCGRG	2 cut(s)	27, 867
AxyI	CCTNAGG	1 cut(s)	2196
BaeI	ACNNNNGTAYC	2 cut(s)	2028, 2061
BalI	TGGCCA	1 cut(s)	1688
BauI	CACGAG	2 cut(s)	963, 2225
BbsI	GAAGAC	3 cut(s)	786, 2208, 2227
Bbv12I	GWGCWC	3 cut(s)	64, 970, 1400
BbvCI	CCTCAGC	2 cut(s)	657, 1314
BccI	CCATC	7 cut(s)	152, 416, 536, 566, 806, 1106, 1882
BcgI	CGANNNNNNTGC	4 cut(s)	848, 882, 1796, 1830
BciT130I	CCWGG	5 cut(s)	66, 706, 887, 1383, 1740
BclI	TGATCA	1 cut(s)	1579
BcoDI	GTCTC	6 cut(s)	24, 510, 937, 1372, 2020, 2222
BfaI	CTAG	9 cut(s)	147, 512, 1065, 1089, 1191, 1212, 1304, 1359, 1469
BfmI	CTRYAG	2 cut(s)	645, 974
BfoI	RGCGCY	1 cut(s)	139
BglI	GCCNNNNNGGC	1 cut(s)	1958
BmcAI	AGTACT	1 cut(s)	319
Bme1390I	CCNGG	5 cut(s)	66, 706, 887, 1383, 1740
BmeRI	GACNNNNNGTC	1 cut(s)	2168
BmeT110I	CYCGRG	2 cut(s)	27, 867
BmgT120I	GGNCC	2 cut(s)	353, 1001
BmiI	GGNNCC	2 cut(s)	1962, 2009
BmrFI	CCNGG	5 cut(s)	66, 706, 887, 1383, 1740
BmsI	GCATC	8 cut(s)	61, 600, 950, 1069, 1444, 1474, 1531, 1684
BmtI	GCTAGC	1 cut(s)	1092
BpiI	GAAGAC	3 cut(s)	786, 2208, 2227
BpmI	CTGGAG	2 cut(s)	30, 869
Bpu10I	CCTNAGC	3 cut(s)	657, 1272, 1314
Bpu14I	TTCGAA	1 cut(s)	1816
BsaBI	GATNNNNATC	1 cut(s)	1464
BsaI	GGTCTC	2 cut(s)	24, 1372
BsaJI	CCNNGG	5 cut(s)	65, 704, 705, 936, 1661
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 280, 310, 867, 897
Bsc4I	CCNNNNNNNGG	1 cut(s)	351
Bse118I	RCCGGY	1 cut(s)	1669
Bse1I	ACTGG	3 cut(s)	345, 1261, 2294
Bse21I	CCTNAGG	1 cut(s)	2196
Bse8I	GATNNNNATC	1 cut(s)	1464
BseBI	CCWGG	5 cut(s)	66, 706, 887, 1383, 1740
BseDI	CCNNGG	5 cut(s)	65, 704, 705, 936, 1661
BseJI	GATNNNNATC	1 cut(s)	1464
BseLI	CCNNNNNNNGG	1 cut(s)	351
BseMII	CTCAG	6 cut(s)	47, 312, 648, 813, 1305, 2187
BseNI	ACTGG	3 cut(s)	345, 1261, 2294
BseRI	GAGGAG	4 cut(s)	301, 674, 1678, 1953
BsgI	GTGCAG	1 cut(s)	139
Bsh1285I	CGRYCG	1 cut(s)	274
BshFI	GGCC	5 cut(s)	344, 355, 1003, 1669, 1688
BsiEI	CGRYCG	1 cut(s)	274
BsiHKAI	GWGCWC	3 cut(s)	64, 970, 1400
BsiHKCI	CYCGRG	2 cut(s)	27, 867
BsiSI	CCGG	1 cut(s)	1670
BslI	CCNNNNNNNGG	1 cut(s)	351
BsmAI	GTCTC	6 cut(s)	24, 510, 937, 1372, 2020, 2222
BsmBI	CGTCTC	2 cut(s)	937, 2222
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	5 cut(s)	344, 355, 1003, 1669, 1688
Bso31I	GGTCTC	2 cut(s)	24, 1372
BsoBI	CYCGRG	2 cut(s)	27, 867
Bsp119I	TTCGAA	1 cut(s)	1816
Bsp1286I	GDGCHC	3 cut(s)	64, 970, 1400
Bsp143I	GATC	5 cut(s)	18, 400, 737, 1579, 2266
Bsp19I	CCATGG	1 cut(s)	936
BspACI	CCGC	3 cut(s)	1076, 1909, 2252
BspANI	GGCC	5 cut(s)	344, 355, 1003, 1669, 1688
BspCNI	CTCAG	6 cut(s)	48, 313, 649, 812, 1306, 2188
BspHI	TCATGA	2 cut(s)	1171, 1243
BspLI	GGNNCC	2 cut(s)	1962, 2009
BspOI	GCTAGC	1 cut(s)	1092
BspPI	GGATC	2 cut(s)	26, 745
BspQI	GCTCTTC	1 cut(s)	159
BspT104I	TTCGAA	1 cut(s)	1816
BspTNI	GGTCTC	2 cut(s)	24, 1372
BsrFI	RCCGGY	1 cut(s)	1669
BsrI	ACTGG	3 cut(s)	345, 1261, 2294
BssAI	RCCGGY	1 cut(s)	1669
BssECI	CCNNGG	5 cut(s)	65, 704, 705, 936, 1661
BssMI	GATC	5 cut(s)	18, 400, 737, 1579, 2266
BssSI	CACGAG	2 cut(s)	963, 2225
BssT1I	CCWWGG	1 cut(s)	936
Bst2BI	CACGAG	2 cut(s)	963, 2225
Bst2UI	CCWGG	5 cut(s)	66, 706, 887, 1383, 1740
Bst6I	CTCTTC	4 cut(s)	86, 159, 681, 1995
BstBI	TTCGAA	1 cut(s)	1816
BstDSI	CCRYGG	1 cut(s)	936
BstH2I	RGCGCY	1 cut(s)	139
BstHHI	GCGC	2 cut(s)	138, 1675
BstKTI	GATC	5 cut(s)	21, 403, 740, 1582, 2269
BstMAI	GTCTC	6 cut(s)	24, 510, 937, 1372, 2020, 2222
BstMBI	GATC	5 cut(s)	18, 400, 737, 1579, 2266
BstMCI	CGRYCG	1 cut(s)	274
BstMWI	GCNNNNNNNGC	7 cut(s)	1235, 1274, 1355, 1723, 1735, 1958, 2191
BstNI	CCWGG	5 cut(s)	66, 706, 887, 1383, 1740
BstNSI	RCATGY	4 cut(s)	1412, 1772, 2185, 2209
BstSCI	CCNGG	5 cut(s)	64, 704, 885, 1381, 1738
BstSFI	CTRYAG	2 cut(s)	645, 974
BstV2I	GAAGAC	3 cut(s)	786, 2208, 2227
BstX2I	RGATCY	1 cut(s)	737
BstYI	RGATCY	1 cut(s)	737
Bsu36I	CCTNAGG	1 cut(s)	2196
BsuRI	GGCC	5 cut(s)	344, 355, 1003, 1669, 1688
BtgI	CCRYGG	1 cut(s)	936
BtsIMutI	CAGTG	4 cut(s)	439, 654, 1744, 1912
CciI	TCATGA	2 cut(s)	1171, 1243
CfoI	GCGC	2 cut(s)	138, 1675
Cfr10I	RCCGGY	1 cut(s)	1669
Cfr13I	GGNCC	2 cut(s)	353, 1001
Csp6I	GTAC	2 cut(s)	318, 2083
CviQI	GTAC	2 cut(s)	318, 2083
DpnI	GATC	5 cut(s)	20, 402, 739, 1581, 2268
DpnII	GATC	5 cut(s)	18, 400, 737, 1579, 2266
DraI	TTTAAA	1 cut(s)	726
DriI	GACNNNNNGTC	1 cut(s)	2168
EaeI	YGGCCR	1 cut(s)	1686
Eam1104I	CTCTTC	4 cut(s)	86, 159, 681, 1995
Eam1105I	GACNNNNNGTC	1 cut(s)	2168
EarI	CTCTTC	4 cut(s)	86, 159, 681, 1995
Eco130I	CCWWGG	1 cut(s)	936
Eco31I	GGTCTC	2 cut(s)	24, 1372
Eco47III	AGCGCT	1 cut(s)	137
Eco57I	CTGAAG	5 cut(s)	642, 705, 863, 1149, 2016
Eco81I	CCTNAGG	1 cut(s)	2196
Eco88I	CYCGRG	2 cut(s)	27, 867
EcoRII	CCWGG	5 cut(s)	64, 704, 885, 1381, 1738
EcoT14I	CCWWGG	1 cut(s)	936
EcoT22I	ATGCAT	1 cut(s)	1467
ErhI	CCWWGG	1 cut(s)	936
Esp3I	CGTCTC	2 cut(s)	937, 2222
FbaI	TGATCA	1 cut(s)	1579
FblI	GTMKAC	1 cut(s)	991
FspBI	CTAG	9 cut(s)	147, 512, 1065, 1089, 1191, 1212, 1304, 1359, 1469
GlaI	GCGC	2 cut(s)	137, 1674
GsuI	CTGGAG	2 cut(s)	30, 869
HaeII	RGCGCY	1 cut(s)	139
HaeIII	GGCC	5 cut(s)	344, 355, 1003, 1669, 1688
HapII	CCGG	1 cut(s)	1670
HhaI	GCGC	2 cut(s)	138, 1675
Hin6I	GCGC	2 cut(s)	136, 1673
HinP1I	GCGC	2 cut(s)	136, 1673
HincII	GTYRAC	2 cut(s)	923, 1792
HindII	GTYRAC	2 cut(s)	923, 1792
HindIII	AAGCTT	1 cut(s)	1117
HinfI	GANTC	7 cut(s)	289, 329, 508, 1025, 1061, 1426, 2065
HpaII	CCGG	1 cut(s)	1670
HphI	GGTGA	3 cut(s)	59, 1582, 2297
Hpy166II	GTNNAC	4 cut(s)	923, 992, 1792, 2122
Hpy188III	TCNNGA	7 cut(s)	749, 869, 1172, 1244, 1825, 1877, 2270
Hpy8I	GTNNAC	4 cut(s)	923, 992, 1792, 2122
Hpy99I	CGWCG	2 cut(s)	1799, 2217
HpyCH4IV	ACGT	1 cut(s)	2124
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1235, 1274, 1355, 1723, 1735, 1958, 2191
HpySE526I	ACGT	1 cut(s)	2124
HspAI	GCGC	2 cut(s)	136, 1673
KroI	GCCGGC	1 cut(s)	1669
KroNI	GCCGGC	1 cut(s)	1671
Ksp22I	TGATCA	1 cut(s)	1579
Kzo9I	GATC	5 cut(s)	18, 400, 737, 1579, 2266
LguI	GCTCTTC	1 cut(s)	159
LmnI	GCTCC	6 cut(s)	67, 661, 888, 1549, 1966, 2007
LweI	GCATC	8 cut(s)	61, 600, 950, 1069, 1444, 1474, 1531, 1684
MaeI	CTAG	9 cut(s)	147, 512, 1065, 1089, 1191, 1212, 1304, 1359, 1469
MaeII	ACGT	1 cut(s)	2124
MaeIII	GTNAC	3 cut(s)	1040, 1588, 1742
MalI	GATC	5 cut(s)	20, 402, 739, 1581, 2268
MboI	GATC	5 cut(s)	18, 400, 737, 1579, 2266
MfeI	CAATTG	2 cut(s)	1983, 2189
MflI	RGATCY	1 cut(s)	737
MhlI	GDGCHC	3 cut(s)	64, 970, 1400
MlsI	TGGCCA	1 cut(s)	1688
MluNI	TGGCCA	1 cut(s)	1688
MlyI	GAGTC	2 cut(s)	298, 517
MmeI	TCCRAC	3 cut(s)	294, 806, 877
Mox20I	TGGCCA	1 cut(s)	1688
Mph1103I	ATGCAT	1 cut(s)	1467
MroNI	GCCGGC	1 cut(s)	1669
MroXI	GAANNNNTTC	1 cut(s)	1134
MscI	TGGCCA	1 cut(s)	1688
MseI	TTAA	6 cut(s)	368, 713, 725, 1094, 1709, 2256
MslI	CAYNNNNRTG	2 cut(s)	57, 1170
Msp20I	TGGCCA	1 cut(s)	1688
MspI	CCGG	1 cut(s)	1670
MspR9I	CCNGG	5 cut(s)	66, 706, 887, 1383, 1740
MunI	CAATTG	2 cut(s)	1983, 2189
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	5 cut(s)	66, 706, 887, 1383, 1740
MwoI	GCNNNNNNNGC	7 cut(s)	1235, 1274, 1355, 1723, 1735, 1958, 2191
NaeI	GCCGGC	1 cut(s)	1671
NcoI	CCATGG	1 cut(s)	936
NdeII	GATC	5 cut(s)	18, 400, 737, 1579, 2266
NgoMIV	GCCGGC	1 cut(s)	1669
NheI	GCTAGC	1 cut(s)	1088
NlaIV	GGNNCC	2 cut(s)	1962, 2009
NmeAIII	GCCGAG	1 cut(s)	1029
NmuCI	GTSAC	3 cut(s)	1040, 1588, 1742
NsiI	ATGCAT	1 cut(s)	1467
NspI	RCATGY	4 cut(s)	1412, 1772, 2185, 2209
NspV	TTCGAA	1 cut(s)	1816
PaeI	GCATGC	1 cut(s)	2185
PaeR7I	CTCGAG	1 cut(s)	867
PagI	TCATGA	2 cut(s)	1171, 1243
PasI	CCCWGGG	1 cut(s)	705
PciI	ACATGT	2 cut(s)	1408, 2205
PciSI	GCTCTTC	1 cut(s)	159
PcsI	WCGNNNNNNNCGW	1 cut(s)	874
PctI	GAATGC	1 cut(s)	658
PdiI	GCCGGC	1 cut(s)	1671
PdmI	GAANNNNTTC	1 cut(s)	1134
PfeI	GAWTC	5 cut(s)	329, 1025, 1061, 1426, 2065
PfoI	TCCNGGA	1 cut(s)	885
PleI	GAGTC	2 cut(s)	297, 516
PpsI	GAGTC	2 cut(s)	297, 516
PscI	ACATGT	2 cut(s)	1408, 2205
Psp6I	CCWGG	5 cut(s)	64, 704, 885, 1381, 1738
PspGI	CCWGG	5 cut(s)	64, 704, 885, 1381, 1738
PspN4I	GGNNCC	2 cut(s)	1962, 2009
PspPI	GGNCC	2 cut(s)	353, 1001
PsrI	GAACNNNNNNTAC	2 cut(s)	1481, 1513
PsuI	RGATCY	1 cut(s)	737
RsaI	GTAC	2 cut(s)	319, 2084
RsaNI	GTAC	2 cut(s)	318, 2083
RseI	CAYNNNNRTG	2 cut(s)	57, 1170
SapI	GCTCTTC	1 cut(s)	159
SaqAI	TTAA	6 cut(s)	368, 713, 725, 1094, 1709, 2256
Sau3AI	GATC	5 cut(s)	18, 400, 737, 1579, 2266
Sau96I	GGNCC	2 cut(s)	353, 1001
ScaI	AGTACT	1 cut(s)	319
SchI	GAGTC	2 cut(s)	298, 517
ScrFI	CCNGG	5 cut(s)	66, 706, 887, 1383, 1740
SduI	GDGCHC	3 cut(s)	64, 970, 1400
SfaNI	GCATC	8 cut(s)	61, 600, 950, 1069, 1444, 1474, 1531, 1684
SfcI	CTRYAG	2 cut(s)	645, 974
Sfr274I	CTCGAG	1 cut(s)	867
SfuI	TTCGAA	1 cut(s)	1816
SlaI	CTCGAG	1 cut(s)	867
SmiMI	CAYNNNNRTG	2 cut(s)	57, 1170
SmlI	CTYRAG	1 cut(s)	867
SmoI	CTYRAG	1 cut(s)	867
SphI	GCATGC	1 cut(s)	2185
SsiI	CCGC	3 cut(s)	1076, 1909, 2252
SspMI	CTAG	9 cut(s)	147, 512, 1065, 1089, 1191, 1212, 1304, 1359, 1469
StyD4I	CCNGG	5 cut(s)	64, 704, 885, 1381, 1738
StyI	CCWWGG	1 cut(s)	936
TaiI	ACGT	1 cut(s)	2127
TaqI	TCGA	5 cut(s)	868, 1615, 1816, 2063, 2265
TaqII	GACCGA	1 cut(s)	288
TatI	WGTACW	1 cut(s)	317
TauI	GCSGC	1 cut(s)	1911
TfiI	GAWTC	5 cut(s)	329, 1025, 1061, 1426, 2065
Tru1I	TTAA	6 cut(s)	368, 713, 725, 1094, 1709, 2256
Tru9I	TTAA	6 cut(s)	368, 713, 725, 1094, 1709, 2256
TscAI	CASTG	4 cut(s)	439, 654, 1751, 1919
TseFI	GTSAC	3 cut(s)	1040, 1588, 1742
Tsp45I	GTSAC	3 cut(s)	1040, 1588, 1742
TspGWI	ACGGA	3 cut(s)	483, 750, 2070
TspRI	CASTG	4 cut(s)	439, 654, 1751, 1919
XapI	RAATTY	4 cut(s)	533, 593, 1812, 1819
XceI	RCATGY	4 cut(s)	1412, 1772, 2185, 2209
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2096
XhoI	CTCGAG	1 cut(s)	867
XmiI	GTMKAC	1 cut(s)	991
XmnI	GAANNNNTTC	1 cut(s)	1134
XspI	CTAG	9 cut(s)	147, 512, 1065, 1089, 1191, 1212, 1304, 1359, 1469
ZrmI	AGTACT	1 cut(s)	319
Zsp2I	ATGCAT	1 cut(s)	1467

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.