pycom16g21600

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0017299
Physical Location & Seq
Reverse (-)
20192452 .. 20194845
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGATTACTGGCGATCAACTTGCCATTGCCAAGGAAACTGGCCGGAGACTTGGAATGGGAACAAACATGTACCCATCTGCTTCCTTACTTGGTCAAGACAAGGATGCTAGCATAGCTGCCCTTCCAATTGAAGAGTTAATTGAGAAGGCTGACGGGTTCGCTGGAGTGTTTCCAGAGCACAAATACGAAATTGTGAAGAAGTTGCAGGAAAAAAAGCACATTTGTGGAATGACGGGAGATGGTGTCAATGATGCCCCTGCATTGAAGAAGGCAGATATTGGAATTGCTGTTGCTGATGCTACAGATGCTGCACGAAGTGCTTCTGACATTGTTCTTACAGAACCCGGACTGAGTGTCATCATTAGTGCAGTGTTGACCAGTAGAGCTATTTTCCAGAGAATGAAGAACTACACAATCTATGCGGTTTCGATCACCATTCGTATTGTGTTTGGTTTCATGTTCATTGCTCTCATATGGAAGTTCGACTTCTCACCTTTCATGGTTTTGATTATTGCCATATTGAACGATGGCACAATTATGACAATATCAAAGGATAGAGTAAAGCCATCTCCCCTGCCTGATAGCTGGAAATTGAAAGAGATTTTTGCTACCGGGATTGTGCTTGGCGGTTACTTGGCATTGATGACTGTTATCTTCTTCTGGTTAATCAAAGAAACTGACTTCTTCTCCGACCAATTTGGTGTAAGATCCTTAAGGGAGACTCCTGAGGAACTAATGGCTGCCTTGTACCTGCAAGTGAGTATTGTAAGCCAGGCCCTCATTTTTGTGACTCGATCCCGCGGCTTCTCCTTTATGGAACGCCCTGGAATGCTTCTACTCGGTGCTTTCATGATCGCGCAGCTGATTGCAACTTTGATAGCAGTATATGCCAACTGGAGTTTTGCAAGAATACACGGATGTGGTTGGGGATGGGCTGGTGTCATCTGGATTTACAGCATTGTCTTCTATTTCCCACTAGACTTGATGAAATTCGCCATCCGTTACATCTTGAGTGGAAAGGCGTGGCTCAACTTGCTAGAGAACAAGACTGCCTTTACCACCAAGAAAGATTATGGAAAAGAAGAGAGAGAAGCTCAATGGGCTCATGCTCAAAGGACCCTTCACGGTCTCGAAGCACCAGAATCTTCTAGTATCTTCAATGACAAGAGCAGCTACAGAGAGCTGTCTGAGATTGCTGAGCAGGCCAAGAGACGAGCTGAAGTTGCAAGACTTCGGGAGCTTCACACGCTCAAGGGACATGTGGAATCAGTTGTGAAGCTAAAAGGCCTGGACATTGACACAATCCAGCAGCATTATACAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

442

Amino Acids

49.3

Weight (kDa)

8.28

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 10 - 92 6.7e-07 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 759
AccII CGCG 2 cut(s) 801, 857
AciI CCGC 4 cut(s) 422, 627, 799, 801
AclWI GGATC 2 cut(s) 702, 789
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 1 cut(s) 989
AcuI CTGAAG 1 cut(s) 1239
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 2 cut(s) 71, 749
AflII CTTAAG 1 cut(s) 712
AflIII ACRYGT 2 cut(s) 66, 1258
AgsI TTSAA 5 cut(s) 131, 265, 523, 595, 1159
AhdI GACNNNNNGTC 1 cut(s) 353
AjnI CCWGG 3 cut(s) 771, 823, 1287
AleI CACNNNNGTG 2 cut(s) 222, 918
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 4 cut(s) 40, 713, 1133, 1204
AlwI GGATC 2 cut(s) 702, 789
AlwNI CAGNNNCTG 1 cut(s) 308
AoxI GGCC 4 cut(s) 40, 774, 1203, 1285
ApeKI GCWGC 6 cut(s) 116, 308, 740, 859, 1170, 1309
ApoI RAATTY 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 319
AspLEI GCGC 1 cut(s) 859
AspS9I GGNCC 2 cut(s) 775, 1116
AsuC2I CCSGG 2 cut(s) 345, 613
AsuHPI GGTGA 2 cut(s) 424, 483
AsuNHI GCTAGC 1 cut(s) 107
AvaII GGWCC 1 cut(s) 1116
AxyI CCTNAGG 1 cut(s) 726
BanII GRGCYC 1 cut(s) 1105
BbsI GAAGAC 1 cut(s) 955
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 6 cut(s) 103, 295, 727, 871, 1182, 1321
BccI CCATC 6 cut(s) 82, 233, 521, 574, 924, 1004
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 773, 825, 1289
BcnI CCSGG 2 cut(s) 345, 613
BcoDI GTCTC 4 cut(s) 40, 713, 1133, 1204
BfaI CTAG 4 cut(s) 108, 977, 1037, 1149
BfmI CTRYAG 2 cut(s) 300, 1174
BfrI CTTAAG 1 cut(s) 712
BfuAI ACCTGC 1 cut(s) 759
BisI GCNGC 7 cut(s) 117, 309, 741, 802, 860, 1171, 1310
BlpI GCTNAGC 1 cut(s) 1197
BlsI GCNGC 7 cut(s) 118, 310, 742, 803, 861, 1172, 1311
Bme1390I CCNGG 5 cut(s) 345, 613, 773, 825, 1289
Bme18I GGWCC 1 cut(s) 1116
BmeRI GACNNNNNGTC 1 cut(s) 353
BmgT120I GGNCC 2 cut(s) 775, 1116
BmiI GGNNCC 1 cut(s) 1118
BmrFI CCNGG 5 cut(s) 345, 613, 773, 825, 1289
BmsI GCATC 4 cut(s) 94, 241, 286, 295
BmtI GCTAGC 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 955
BplI GAGNNNNNCTC 2 cut(s) 1078, 1110
BpmI CTGGAG 2 cut(s) 183, 916
Bpu1102I GCTNAGC 1 cut(s) 1197
BpuEI CTTGAG 2 cut(s) 1030, 1235
BpuMI CCSGG 2 cut(s) 345, 613
BsaI GGTCTC 1 cut(s) 1133
BsaJI CCNNGG 3 cut(s) 30, 799, 823
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 671, 701
Bse1I ACTGG 4 cut(s) 13, 43, 378, 899
Bse21I CCTNAGG 1 cut(s) 726
Bse3DI GCAATG 2 cut(s) 24, 462
BseBI CCWGG 3 cut(s) 773, 825, 1289
BseDI CCNNGG 3 cut(s) 30, 799, 823
BseGI GGATG 4 cut(s) 109, 923, 935, 996
BseMI GCAATG 2 cut(s) 24, 462
BseMII CTCAG 4 cut(s) 341, 717, 1179, 1188
BseNI ACTGG 4 cut(s) 13, 43, 378, 899
BseXI GCAGC 6 cut(s) 103, 295, 727, 871, 1182, 1321
BsgI GTGCAG 2 cut(s) 294, 387
Bsh1236I CGCG 2 cut(s) 801, 857
BshFI GGCC 4 cut(s) 42, 776, 1205, 1287
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 3 cut(s) 43, 345, 612
BslFI GGGAC 1 cut(s) 1269
BsmAI GTCTC 4 cut(s) 40, 713, 1133, 1204
BsmBI CGTCTC 1 cut(s) 1204
BsmFI GGGAC 1 cut(s) 1269
BsmI GAATGC 1 cut(s) 834
BsnI GGCC 4 cut(s) 42, 776, 1205, 1287
Bso31I GGTCTC 1 cut(s) 1133
Bsp1286I GDGCHC 2 cut(s) 180, 1105
Bsp143I GATC 5 cut(s) 13, 429, 707, 794, 852
Bsp1720I GCTNAGC 1 cut(s) 1197
BspACI CCGC 4 cut(s) 422, 627, 799, 801
BspANI GGCC 4 cut(s) 42, 776, 1205, 1287
BspCNI CTCAG 4 cut(s) 342, 718, 1180, 1189
BspFNI CGCG 2 cut(s) 801, 857
BspHI TCATGA 1 cut(s) 849
BspLI GGNNCC 1 cut(s) 1118
BspMI ACCTGC 1 cut(s) 759
BspOI GCTAGC 1 cut(s) 111
BspPI GGATC 2 cut(s) 702, 789
BspTI CTTAAG 1 cut(s) 712
BspTNI GGTCTC 1 cut(s) 1133
BsrDI GCAATG 2 cut(s) 24, 462
BsrI ACTGG 4 cut(s) 13, 43, 378, 899
BssECI CCNNGG 3 cut(s) 30, 799, 823
BssMI GATC 5 cut(s) 13, 429, 707, 794, 852
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 3 cut(s) 773, 825, 1289
Bst4CI ACNGT 3 cut(s) 649, 1127, 1321
Bst6I CTCTTC 2 cut(s) 126, 1077
BstAFI CTTAAG 1 cut(s) 712
BstAPI GCANNNNNTGC 2 cut(s) 317, 887
BstC8I GCNNGC 2 cut(s) 109, 1203
BstDEI CTNAG 4 cut(s) 350, 726, 1188, 1197
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 4 cut(s) 109, 923, 935, 996
BstFNI CGCG 2 cut(s) 801, 857
BstHHI GCGC 1 cut(s) 859
BstKTI GATC 5 cut(s) 16, 432, 710, 797, 855
BstMAI GTCTC 4 cut(s) 40, 713, 1133, 1204
BstMBI GATC 5 cut(s) 13, 429, 707, 794, 852
BstMWI GCNNNNNNNGC 9 cut(s) 113, 305, 317, 887, 1033, 1100, 1202, 1223, 1246
BstNI CCWGG 3 cut(s) 773, 825, 1289
BstNSI RCATGY 2 cut(s) 70, 1262
BstSCI CCNGG 5 cut(s) 343, 611, 771, 823, 1287
BstSFI CTRYAG 2 cut(s) 300, 1174
BstUI CGCG 2 cut(s) 801, 857
BstV1I GCAGC 6 cut(s) 103, 295, 727, 871, 1182, 1321
BstV2I GAAGAC 1 cut(s) 955
BstX2I RGATCY 1 cut(s) 707
BstYI RGATCY 1 cut(s) 707
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 4 cut(s) 42, 776, 1205, 1287
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 4 cut(s) 109, 923, 935, 996
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 1326
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 2 cut(s) 109, 1203
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 1 cut(s) 849
CfoI GCGC 1 cut(s) 859
Cfr13I GGNCC 2 cut(s) 775, 1116
Cfr42I CCGCGG 1 cut(s) 802
Csp6I GTAC 2 cut(s) 70, 748
CspCI CAANNNNNGTGG 2 cut(s) 963, 998
CviAII CATG 6 cut(s) 67, 457, 499, 850, 1106, 1259
CviQI GTAC 2 cut(s) 70, 748
DdeI CTNAG 4 cut(s) 350, 726, 1188, 1197
DpnI GATC 5 cut(s) 15, 431, 709, 796, 854
DpnII GATC 5 cut(s) 13, 429, 707, 794, 852
DraIII CACNNNGTG 1 cut(s) 317
DriI GACNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 126, 1077
Eam1105I GACNNNNNGTC 1 cut(s) 353
EarI CTCTTC 2 cut(s) 126, 1077
Eco130I CCWWGG 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 1287
Eco24I GRGCYC 1 cut(s) 1105
Eco31I GGTCTC 1 cut(s) 1133
Eco47I GGWCC 1 cut(s) 1116
Eco57I CTGAAG 1 cut(s) 1239
Eco81I CCTNAGG 1 cut(s) 726
EcoO109I RGGNCCY 2 cut(s) 775, 1116
EcoRII CCWGG 3 cut(s) 771, 823, 1287
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 1105
ErhI CCWWGG 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 1204
FaeI CATG 6 cut(s) 70, 460, 502, 853, 1109, 1262
FalI AAGNNNNNCTT 4 cut(s) 470, 502, 1037, 1069
FaqI GGGAC 1 cut(s) 1269
FatI CATG 6 cut(s) 66, 456, 498, 849, 1105, 1258
FauI CCCGC 1 cut(s) 806
FauNDI CATATG 1 cut(s) 473
Fnu4HI GCNGC 7 cut(s) 117, 309, 741, 802, 860, 1171, 1310
FokI GGATG 4 cut(s) 116, 930, 942, 983
FriOI GRGCYC 1 cut(s) 1105
Fsp4HI GCNGC 7 cut(s) 117, 309, 741, 802, 860, 1171, 1310
FspBI CTAG 4 cut(s) 108, 977, 1037, 1149
GlaI GCGC 1 cut(s) 858
GluI GCNGC 7 cut(s) 117, 309, 741, 802, 860, 1171, 1310
GsuI CTGGAG 2 cut(s) 183, 916
HaeIII GGCC 4 cut(s) 42, 776, 1205, 1287
HapII CCGG 3 cut(s) 43, 345, 612
HhaI GCGC 1 cut(s) 859
Hin1II CATG 6 cut(s) 70, 460, 502, 853, 1109, 1262
Hin6I GCGC 1 cut(s) 857
HinP1I GCGC 1 cut(s) 857
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 4 cut(s) 721, 790, 1142, 1265
HpaII CCGG 3 cut(s) 43, 345, 612
HphI GGTGA 2 cut(s) 424, 483
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 3 cut(s) 325, 691, 1189
Hpy188III TCNNGA 9 cut(s) 95, 173, 394, 725, 850, 946, 1009, 1130, 1235
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 4 cut(s) 131, 139, 262, 1130
HpyCH4III ACNGT 3 cut(s) 649, 1127, 1321
HpyCH4V TGCA 8 cut(s) 205, 260, 311, 368, 754, 869, 905, 1226
HpyF10VI GCNNNNNNNGC 9 cut(s) 113, 305, 317, 887, 1033, 1100, 1202, 1223, 1246
HpyF3I CTNAG 4 cut(s) 350, 726, 1188, 1197
Hsp92II CATG 6 cut(s) 70, 460, 502, 853, 1109, 1262
HspAI GCGC 1 cut(s) 857
KspI CCGCGG 1 cut(s) 802
Kzo9I GATC 5 cut(s) 13, 429, 707, 794, 852
LmnI GCTCC 1 cut(s) 1237
Lsp1109I GCAGC 6 cut(s) 103, 295, 727, 871, 1182, 1321
LweI GCATC 4 cut(s) 94, 241, 286, 295
MaeI CTAG 4 cut(s) 108, 977, 1037, 1149
MaeIII GTNAC 3 cut(s) 629, 787, 1001
MalI GATC 5 cut(s) 15, 431, 709, 796, 854
MboI GATC 5 cut(s) 13, 429, 707, 794, 852
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 2 cut(s) 180, 1105
MluCI AATT 8 cut(s) 126, 138, 189, 282, 534, 590, 695, 989
MlyI GAGTC 2 cut(s) 715, 784
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 2 cut(s) 721, 788
MroXI GAANNNNTTC 1 cut(s) 319
MseI TTAA 3 cut(s) 137, 665, 713
MslI CAYNNNNRTG 4 cut(s) 222, 536, 785, 918
MspA1I CMGCKG 2 cut(s) 801, 862
MspCI CTTAAG 1 cut(s) 712
MspI CCGG 3 cut(s) 43, 345, 612
MspR9I CCNGG 5 cut(s) 345, 613, 773, 825, 1289
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 834
MvaI CCWGG 3 cut(s) 773, 825, 1289
MvnI CGCG 2 cut(s) 801, 857
MwoI GCNNNNNNNGC 9 cut(s) 113, 305, 317, 887, 1033, 1100, 1202, 1223, 1246
NciI CCSGG 2 cut(s) 345, 613
NdeI CATATG 1 cut(s) 473
NdeII GATC 5 cut(s) 13, 429, 707, 794, 852
NheI GCTAGC 1 cut(s) 107
NlaIII CATG 6 cut(s) 70, 460, 502, 853, 1109, 1262
NlaIV GGNNCC 1 cut(s) 1118
NmuCI GTSAC 1 cut(s) 787
NspI RCATGY 2 cut(s) 70, 1262
OliI CACNNNNGTG 2 cut(s) 222, 918
PagI TCATGA 1 cut(s) 849
PceI AGGCCT 1 cut(s) 1287
PciI ACATGT 2 cut(s) 66, 1258
PctI GAATGC 1 cut(s) 834
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 1142, 1265
PkrI GCNGC 7 cut(s) 118, 310, 742, 803, 861, 1172, 1311
PleI GAGTC 2 cut(s) 715, 784
PpsI GAGTC 2 cut(s) 715, 784
PpuMI RGGWCCY 1 cut(s) 1116
PscI ACATGT 2 cut(s) 66, 1258
Psp5II RGGWCCY 1 cut(s) 1116
Psp6I CCWGG 3 cut(s) 771, 823, 1287
PspGI CCWGG 3 cut(s) 771, 823, 1287
PspN4I GGNNCC 1 cut(s) 1118
PspPI GGNCC 2 cut(s) 775, 1116
PspPPI RGGWCCY 1 cut(s) 1116
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 707
PvuII CAGCTG 1 cut(s) 862
RsaI GTAC 2 cut(s) 71, 749
RsaNI GTAC 2 cut(s) 70, 748
RseI CAYNNNNRTG 4 cut(s) 222, 536, 785, 918
SacII CCGCGG 1 cut(s) 802
SaqAI TTAA 3 cut(s) 137, 665, 713
SatI GCNGC 7 cut(s) 117, 309, 741, 802, 860, 1171, 1310
Sau3AI GATC 5 cut(s) 13, 429, 707, 794, 852
Sau96I GGNCC 2 cut(s) 775, 1116
SchI GAGTC 2 cut(s) 715, 784
ScrFI CCNGG 5 cut(s) 345, 613, 773, 825, 1289
SduI GDGCHC 2 cut(s) 180, 1105
SfaNI GCATC 4 cut(s) 94, 241, 286, 295
SfcI CTRYAG 2 cut(s) 300, 1174
Sfr303I CCGCGG 1 cut(s) 802
SgrBI CCGCGG 1 cut(s) 802
SinI GGWCC 1 cut(s) 1116
SmiMI CAYNNNNRTG 4 cut(s) 222, 536, 785, 918
SmlI CTYRAG 3 cut(s) 712, 1009, 1250
SmoI CTYRAG 3 cut(s) 712, 1009, 1250
Sse9I AATT 8 cut(s) 126, 138, 189, 282, 534, 590, 695, 989
SseBI AGGCCT 1 cut(s) 1287
SsiI CCGC 4 cut(s) 422, 627, 799, 801
SspMI CTAG 4 cut(s) 108, 977, 1037, 1149
StuI AGGCCT 1 cut(s) 1287
StyD4I CCNGG 5 cut(s) 343, 611, 771, 823, 1287
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 3 cut(s) 649, 1127, 1321
TaqI TCGA 4 cut(s) 428, 483, 793, 1131
TasI AATT 8 cut(s) 126, 138, 189, 282, 534, 590, 695, 989
TauI GCSGC 1 cut(s) 804
TfiI GAWTC 2 cut(s) 1142, 1265
Tru1I TTAA 3 cut(s) 137, 665, 713
Tru9I TTAA 3 cut(s) 137, 665, 713
TscAI CASTG 2 cut(s) 375, 1326
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 6 cut(s) 116, 308, 740, 859, 1170, 1309
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 6 cut(s) 416, 445, 451, 487, 838, 1001
TspGWI ACGGA 2 cut(s) 930, 989
TspRI CASTG 2 cut(s) 375, 1326
Vha464I CTTAAG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 1116
XapI RAATTY 1 cut(s) 989
XceI RCATGY 2 cut(s) 70, 1262
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 4 cut(s) 108, 977, 1037, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.