pycom17g00460
NAC Family

inactive shikimate kinase like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013212
Physical Location & Seq
Reverse (-)
323733 .. 325359
1627 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
ATGGAGATAACAAGGAGCTTCCTCCTCCATTCTTCTTGCTCCAAGCTCTCATTCCGCACTCCCTTACGCCACTCTCGAGTTGTGGCTTCCAGTTTCTCAATTCCGATTACCTCTCGCACCCACATCCGAAAACCTCTCGCCGCTTCATGCTCCGCCTTCGACGGCGGCGATTCCAGCTCAGCAGCCAAGGTTTCTGTAGCTGACCTCTCTTCTGCAGTAAAGAACAAGGCAATGGATATAACCCCTGAGCTGAAAGGAACTTCCATATTTCTTGTGGGCATGAAGAGCTCCATGAAAACCAGTTTGGGGAAGCTCCTAGCCAATGTGTTGCAATATTATTACTTTGACAGTGATAGTTTGGTTGAGGAAGCTGCAGGAGGTGAATCTGCTGCCAAATTGCTTACGGTGGCTGACACAAATGGTTTTCGGGAATCCGAGACTGAAGTATTGAAGCAACTGTCATCCATGGATCGATTGGTGGTTTGTGCCGGAGATGGTGCAGTTCAAAGTTCAACTAATCTGGCTCTTCTGAGGTATGGAATCTCTATATGGATTGATGTACCTGTAGACTTGGTGGCCAGGGGCATGATCGAAGATCAAAGTCAGCTTCCTGCACTCAACTTATCTGCTTCAGCATCTTATCAAGAGGTTTTGACTCACCTAAGTACTTCGTACGAAGAAGTGAGAGGTGGATACGAAACAGCTGATGCAACCGTTTCCGTTGAAAAACTAGCTTATAAATTAGGTTATGATGACCTTGGTGATGTAACGACAGAAAACATGGATTTGGAGGTAACGAGTGTAGTTTTGGTATAG

Protein Analysis

272

Amino Acids

29.06

Weight (kDa)

4.99

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 98 - 229 4.2e-24 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26900 AT3G26900 AT3G26900
fragaria_vesca FvH4_6g54030 FvH4_6g54030
malus_domestica MD09G1006100.v1.1 MD17G1010100.v1.1
prunus_persica Prupe.3G310900_v2.0.a1
pyrus_communis pycom17g00460
rosa_chinensis RchiOBHm_Chr2g0176211
rosa_laevigata RLG00000022390
rosa_multiflora Rmu_co8135912.1_g000001
rosa_rugosa Rorug02G0591800
rosa_samantha Rh2AG670400 Rh2BG681900 Rh2CG644700 Rh2DG695500
rosa_wichuraiana Rw2G054990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AccI GTMKAC 1 cut(s) 567
AciI CCGC 4 cut(s) 55, 141, 153, 165
AclWI GGATC 1 cut(s) 477
AcoI YGGCCR 1 cut(s) 576
AcuI CTGAAG 2 cut(s) 462, 615
AfaI GTAC 3 cut(s) 561, 667, 674
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 4 cut(s) 451, 506, 513, 725
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 4 cut(s) 35, 67, 287, 319
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 431
AlwI GGATC 1 cut(s) 477
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 1 cut(s) 576
ApeKI GCWGC 3 cut(s) 182, 371, 389
AsuHPI GGTGA 3 cut(s) 392, 650, 773
AvaI CYCGRG 1 cut(s) 75
BalI TGGCCA 1 cut(s) 578
BanII GRGCYC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 194, 358, 376
BccI CCATC 1 cut(s) 488
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 1 cut(s) 580
BciVI GTATCC 1 cut(s) 686
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 2 cut(s) 317, 731
BfmI CTRYAG 4 cut(s) 195, 213, 372, 564
BfuI GTATCC 1 cut(s) 686
BisI GCNGC 5 cut(s) 141, 166, 183, 372, 390
BlpI GCTNAGC 1 cut(s) 178
BlsI GCNGC 5 cut(s) 142, 167, 184, 373, 391
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 644, 697
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 472
BsaJI CCNNGG 4 cut(s) 186, 465, 579, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 2 cut(s) 90, 300
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 580
BseCI ATCGAT 1 cut(s) 472
BseDI CCNNGG 4 cut(s) 186, 465, 579, 757
BseGI GGATG 2 cut(s) 123, 461
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 3 cut(s) 192, 237, 521
BseNI ACTGG 2 cut(s) 90, 300
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 3 cut(s) 194, 358, 376
BsgI GTGCAG 2 cut(s) 519, 597
BshFI GGCC 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 489
BsiWI CGTACG 1 cut(s) 672
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 1 cut(s) 431
BsnI GGCC 1 cut(s) 578
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 3 cut(s) 469, 588, 595
Bsp1720I GCTNAGC 1 cut(s) 178
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 4 cut(s) 55, 141, 153, 165
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 3 cut(s) 191, 238, 522
BspDI ATCGAT 1 cut(s) 472
BspMAI CTGCAG 2 cut(s) 217, 376
BspPI GGATC 1 cut(s) 477
BspQI GCTCTTC 2 cut(s) 278, 531
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 2 cut(s) 90, 300
BssECI CCNNGG 4 cut(s) 186, 465, 579, 757
BssMI GATC 3 cut(s) 469, 588, 595
BssT1I CCWWGG 3 cut(s) 186, 465, 757
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 4 cut(s) 350, 406, 459, 715
Bst6I CTCTTC 3 cut(s) 214, 278, 531
BstDEI CTNAG 4 cut(s) 178, 246, 530, 662
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 123, 461
BstKTI GATC 3 cut(s) 472, 591, 598
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 3 cut(s) 469, 588, 595
BstMWI GCNNNNNNNGC 2 cut(s) 174, 285
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 4 cut(s) 195, 213, 372, 564
BstV1I GCAGC 3 cut(s) 194, 358, 376
Bsu15I ATCGAT 1 cut(s) 472
BsuI GTATCC 1 cut(s) 686
BsuRI GGCC 1 cut(s) 578
BsuTUI ATCGAT 1 cut(s) 472
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 123, 461
BtsIMutI CAGTG 1 cut(s) 355
ClaI ATCGAT 1 cut(s) 472
Csp6I GTAC 3 cut(s) 560, 666, 673
CviAII CATG 6 cut(s) 147, 280, 292, 466, 586, 781
CviQI GTAC 3 cut(s) 560, 666, 673
DdeI CTNAG 4 cut(s) 178, 246, 530, 662
DpnI GATC 3 cut(s) 471, 590, 597
DpnII GATC 3 cut(s) 469, 588, 595
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 3 cut(s) 214, 278, 531
EarI CTCTTC 3 cut(s) 214, 278, 531
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 3 cut(s) 186, 465, 757
Eco24I GRGCYC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 2 cut(s) 462, 615
Eco88I CYCGRG 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 578
EcoT14I CCWWGG 3 cut(s) 186, 465, 757
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 3 cut(s) 186, 465, 757
FaeI CATG 6 cut(s) 150, 283, 295, 469, 589, 784
FatI CATG 6 cut(s) 146, 279, 291, 465, 585, 780
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 5 cut(s) 141, 166, 183, 372, 390
FokI GGATG 2 cut(s) 110, 448
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 5 cut(s) 141, 166, 183, 372, 390
FspBI CTAG 2 cut(s) 317, 731
GluI GCNGC 5 cut(s) 141, 166, 183, 372, 390
HaeIII GGCC 1 cut(s) 578
HapII CCGG 1 cut(s) 489
Hin1II CATG 6 cut(s) 150, 283, 295, 469, 589, 784
HinfI GANTC 5 cut(s) 170, 383, 431, 540, 655
HpaII CCGG 1 cut(s) 489
HphI GGTGA 3 cut(s) 392, 650, 773
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 105, 128, 436, 531
Hpy188III TCNNGA 3 cut(s) 75, 428, 644
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 4 cut(s) 350, 406, 459, 715
HpyCH4V TGCA 6 cut(s) 215, 331, 374, 500, 614, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 285
HpyF3I CTNAG 4 cut(s) 178, 246, 530, 662
Hsp92II CATG 6 cut(s) 150, 283, 295, 469, 589, 784
Kzo9I GATC 3 cut(s) 469, 588, 595
LguI GCTCTTC 2 cut(s) 278, 531
LmnI GCTCC 5 cut(s) 15, 44, 155, 293, 318
Lsp1109I GCAGC 3 cut(s) 194, 358, 376
LweI GCATC 2 cut(s) 644, 697
MaeI CTAG 2 cut(s) 317, 731
MaeIII GTNAC 2 cut(s) 766, 793
MalI GATC 3 cut(s) 471, 590, 597
MboI GATC 3 cut(s) 469, 588, 595
MboII GAAGA 6 cut(s) 24, 201, 295, 518, 605, 689
MhlI GDGCHC 1 cut(s) 290
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 3 cut(s) 99, 395, 740
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 1 cut(s) 649
Mox20I TGGCCA 1 cut(s) 578
MscI TGGCCA 1 cut(s) 578
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 580
MvaI CCWGG 1 cut(s) 580
MwoI GCNNNNNNNGC 2 cut(s) 174, 285
NcoI CCATGG 1 cut(s) 465
NdeII GATC 3 cut(s) 469, 588, 595
NlaIII CATG 6 cut(s) 150, 283, 295, 469, 589, 784
PaeR7I CTCGAG 1 cut(s) 75
PciSI GCTCTTC 2 cut(s) 278, 531
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 165
PfeI GAWTC 4 cut(s) 170, 383, 431, 540
Pfl23II CGTACG 1 cut(s) 672
PkrI GCNGC 5 cut(s) 142, 167, 184, 373, 391
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PsiI TTATAA 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspLI CGTACG 1 cut(s) 672
PstI CTGCAG 2 cut(s) 217, 376
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 3 cut(s) 561, 667, 674
RsaNI GTAC 3 cut(s) 560, 666, 673
SacI GAGCTC 1 cut(s) 290
SapI GCTCTTC 2 cut(s) 278, 531
SatI GCNGC 5 cut(s) 141, 166, 183, 372, 390
Sau3AI GATC 3 cut(s) 469, 588, 595
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 580
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 2 cut(s) 644, 697
SfcI CTRYAG 4 cut(s) 195, 213, 372, 564
Sfr274I CTCGAG 1 cut(s) 75
SlaI CTCGAG 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 3 cut(s) 99, 395, 740
SsiI CCGC 4 cut(s) 55, 141, 153, 165
SspI AATATT 1 cut(s) 335
SspMI CTAG 2 cut(s) 317, 731
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 578
StyI CCWWGG 3 cut(s) 186, 465, 757
TaaI ACNGT 4 cut(s) 350, 406, 459, 715
TaqI TCGA 4 cut(s) 76, 159, 472, 591
TasI AATT 3 cut(s) 99, 395, 740
TatI WGTACW 1 cut(s) 665
TauI GCSGC 2 cut(s) 143, 168
TfiI GAWTC 4 cut(s) 170, 383, 431, 540
TscAI CASTG 1 cut(s) 355
TseI GCWGC 3 cut(s) 182, 371, 389
TspDTI ATGAA 3 cut(s) 135, 296, 308
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 2 cut(s) 271, 472
XhoI CTCGAG 1 cut(s) 75
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 2 cut(s) 317, 731
ZrmI AGTACT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.