pycom17g01960

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008999
Physical Location & Seq
Forward (+)
1240392 .. 1241276
885 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
ATGGAAACTCTTTGCAGGCTTCACGGATGGAAGATTCGGGAATCACCTAGACGAGTCTATGATGCAGTTCTTTTCAGCAATGAAGTAGACATGCTTACAATCCGATGGAATGAATTGTATCCTTTTGTGACACAGTTTGTTCTCCTCGAATCAAACTCAACGTTTACTGGCTTGCCAAAGCCATTGATTTTTGGAAAAAACCGGGAGAAGTTTAAGTTTGTTGAAAACCGGCTTACTTATGTTACGACAGGAGGAAGATTTCAGAAAGGTGTAAACCCATTTTATGAGGAGGCGTATCAAAGAGTGGCACTTGACCAGATTCTTAGAGTTGCTGGTATAGAAGATGATGACCTGCTGATCATGTCCGATGTTGATGAGATTCCTAGTGCCCACACAATTGATCTCTTGAGGTGGTGTGATGACACCCCGCCTGTTGTTCATCTTGGGCTGAGGGATTACTTGTACTCTTTTGAATATTATGTGGATGACAAGAGCTGGAGAGCATCCGTTCACAGGTACCAGACTGGTAATACTACCTATGCACACTTCAGACAGAGCGACTACCTTTTGCCAGATGCAGGTTGGCATTGCAGCTTTTGTTTCCGCCATATCAGCGAGTTCATATTCAAGATGAAAGCTTACAGCCACAATGATCGGGTGAGGTTCTCTCATTATCTGAACCCTCGAAGGATCCAAGATGTAATATGTAAAGGGGCTGATCTATTTGACATGCTTCCCGAGGAGTACACATTTAAGGAGATTATTGGAAAGATGGGACCTATTCCTAGATCTCATTCAGCGGTTCATCTCCCTGCATATTTGTTGAACAATGCTGAGAAGTATAAATACCTATTGCCCGGAAACTGCAAGAGGGAAAATGGCTAA

Protein Analysis

295

Amino Acids

34.62

Weight (kDa)

6.83

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 1 - 292 2.9e-159 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 360, 569
Acc65I GGTACC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 516
AccI GTMKAC 1 cut(s) 87
AciI CCGC 3 cut(s) 428, 604, 800
AclI AACGTT 1 cut(s) 161
AclWI GGATC 2 cut(s) 685, 698
AcuI CTGAAG 1 cut(s) 532
AfaI GTAC 3 cut(s) 464, 518, 746
AfiI CCNNNNNNNGG 2 cut(s) 513, 578
AgsI TTSAA 4 cut(s) 224, 473, 628, 826
AluBI AGCT 3 cut(s) 495, 594, 638
AluI AGCT 3 cut(s) 495, 594, 638
AlwI GGATC 2 cut(s) 685, 698
Ama87I CYCGRG 1 cut(s) 737
ApeKI GCWGC 1 cut(s) 591
Asp718I GGTACC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 776
AsuC2I CCSGG 2 cut(s) 203, 858
AsuHPI GGTGA 2 cut(s) 36, 670
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 776
BaeGI GKGCMC 1 cut(s) 391
BamHI GGATCC 1 cut(s) 690
BanI GGYRCC 1 cut(s) 516
BarI GAAGNNNNNNTAC 2 cut(s) 830, 862
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 1 cut(s) 603
BccI CCATC 3 cut(s) 21, 99, 766
BciVI GTATCC 1 cut(s) 129
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 2 cut(s) 203, 858
BfaI CTAG 3 cut(s) 48, 384, 786
BfuAI ACCTGC 2 cut(s) 360, 569
BfuI GTATCC 1 cut(s) 129
BglII AGATCT 1 cut(s) 788
BisI GCNGC 1 cut(s) 592
BlsI GCNGC 1 cut(s) 593
Bme1390I CCNGG 2 cut(s) 203, 858
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 3 cut(s) 518, 692, 777
BmrFI CCNGG 2 cut(s) 203, 858
BmsI GCATC 3 cut(s) 52, 512, 565
BplI GAGNNNNNCTC 2 cut(s) 652, 684
BpmI CTGGAG 1 cut(s) 517
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 427
BpuMI CCSGG 2 cut(s) 203, 858
BsaJI CCNNGG 1 cut(s) 738
Bsc4I CCNNNNNNNGG 2 cut(s) 513, 578
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 2 cut(s) 172, 529
Bse3DI GCAATG 2 cut(s) 85, 586
BseDI CCNNGG 1 cut(s) 738
BseGI GGATG 3 cut(s) 32, 490, 503
BseLI CCNNNNNNNGG 2 cut(s) 513, 578
BseMI GCAATG 2 cut(s) 85, 586
BseMII CTCAG 2 cut(s) 440, 825
BseNI ACTGG 2 cut(s) 172, 529
BseRI GAGGAG 3 cut(s) 134, 302, 755
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 1 cut(s) 603
BshNI GGYRCC 1 cut(s) 516
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 3 cut(s) 202, 229, 858
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 2 cut(s) 513, 578
BsmFI GGGAC 1 cut(s) 789
BsoBI CYCGRG 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 6 cut(s) 357, 400, 652, 690, 718, 788
BspACI CCGC 3 cut(s) 428, 604, 800
BspCNI CTCAG 2 cut(s) 441, 826
BspLI GGNNCC 3 cut(s) 518, 692, 777
BspMI ACCTGC 2 cut(s) 360, 569
BspPI GGATC 2 cut(s) 685, 698
BspT107I GGYRCC 1 cut(s) 516
BsrDI GCAATG 2 cut(s) 85, 586
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 2 cut(s) 172, 529
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 738
BssMI GATC 6 cut(s) 357, 400, 652, 690, 718, 788
Bst4CI ACNGT 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 17, 173
BstDEI CTNAG 3 cut(s) 323, 449, 834
BstF5I GGATG 3 cut(s) 32, 490, 503
BstKTI GATC 6 cut(s) 360, 403, 655, 693, 721, 791
BstMBI GATC 6 cut(s) 357, 400, 652, 690, 718, 788
BstMWI GCNNNNNNNGC 1 cut(s) 612
BstNSI RCATGY 2 cut(s) 94, 733
BstSCI CCNGG 2 cut(s) 201, 856
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 603
BstX2I RGATCY 2 cut(s) 690, 788
BstYI RGATCY 2 cut(s) 690, 788
BsuI GTATCC 1 cut(s) 129
BtsCI GGATG 3 cut(s) 32, 490, 503
BveI ACCTGC 2 cut(s) 360, 569
Cac8I GCNNGC 2 cut(s) 17, 173
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 776
Csp6I GTAC 3 cut(s) 463, 517, 745
CviAII CATG 3 cut(s) 91, 361, 730
CviQI GTAC 3 cut(s) 463, 517, 745
DdeI CTNAG 3 cut(s) 323, 449, 834
DpnI GATC 6 cut(s) 359, 402, 654, 692, 720, 790
DpnII GATC 6 cut(s) 357, 400, 652, 690, 718, 788
EciI GGCGGA 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 776
FaeI CATG 3 cut(s) 94, 364, 733
FaqI GGGAC 1 cut(s) 789
FatI CATG 3 cut(s) 90, 360, 729
FauI CCCGC 1 cut(s) 435
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 592
FokI GGATG 3 cut(s) 39, 490, 497
Fsp4HI GCNGC 1 cut(s) 592
FspBI CTAG 3 cut(s) 48, 384, 786
GluI GCNGC 1 cut(s) 592
GsuI CTGGAG 1 cut(s) 517
HapII CCGG 3 cut(s) 202, 229, 858
Hin1II CATG 3 cut(s) 94, 364, 733
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 6 cut(s) 34, 41, 54, 149, 319, 379
HpaII CCGG 3 cut(s) 202, 229, 858
HphI GGTGA 2 cut(s) 36, 670
Hpy166II GTNNAC 5 cut(s) 88, 165, 274, 511, 747
Hpy188I TCNGA 5 cut(s) 104, 264, 367, 551, 678
Hpy188III TCNNGA 4 cut(s) 38, 406, 628, 737
Hpy8I GTNNAC 5 cut(s) 88, 165, 274, 511, 747
HpyAV CCTTC 1 cut(s) 681
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 7 cut(s) 15, 65, 542, 578, 591, 815, 867
HpyF10VI GCNNNNNNNGC 1 cut(s) 612
HpyF3I CTNAG 3 cut(s) 323, 449, 834
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 3 cut(s) 94, 364, 733
KpnI GGTACC 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 6 cut(s) 357, 400, 652, 690, 718, 788
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 3 cut(s) 52, 512, 565
MaeI CTAG 3 cut(s) 48, 384, 786
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 127, 241
MalI GATC 6 cut(s) 359, 402, 654, 692, 720, 790
MboI GATC 6 cut(s) 357, 400, 652, 690, 718, 788
MboII GAAGA 3 cut(s) 43, 267, 353
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 2 cut(s) 690, 788
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 2 cut(s) 113, 396
MlyI GAGTC 1 cut(s) 63
MseI TTAA 2 cut(s) 213, 753
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 3 cut(s) 202, 229, 858
MspR9I CCNGG 2 cut(s) 203, 858
MunI CAATTG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 612
NciI CCSGG 2 cut(s) 203, 858
NdeII GATC 6 cut(s) 357, 400, 652, 690, 718, 788
NlaIII CATG 3 cut(s) 94, 364, 733
NlaIV GGNNCC 3 cut(s) 518, 692, 777
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 2 cut(s) 94, 733
PfeI GAWTC 5 cut(s) 34, 41, 149, 319, 379
PkrI GCNGC 1 cut(s) 593
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 776
Psp1406I AACGTT 1 cut(s) 161
Psp5II RGGWCCY 1 cut(s) 776
PspN4I GGNNCC 3 cut(s) 518, 692, 777
PspPI GGNCC 1 cut(s) 776
PspPPI RGGWCCY 1 cut(s) 776
PsuI RGATCY 2 cut(s) 690, 788
RsaI GTAC 3 cut(s) 464, 518, 746
RsaNI GTAC 3 cut(s) 463, 517, 745
SaqAI TTAA 2 cut(s) 213, 753
SatI GCNGC 1 cut(s) 592
Sau3AI GATC 6 cut(s) 357, 400, 652, 690, 718, 788
Sau96I GGNCC 1 cut(s) 776
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 2 cut(s) 203, 858
SduI GDGCHC 1 cut(s) 391
SfaNI GCATC 3 cut(s) 52, 512, 565
SinI GGWCC 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 406
SmoI CTYRAG 1 cut(s) 406
Sse9I AATT 2 cut(s) 113, 396
SsiI CCGC 3 cut(s) 428, 604, 800
SspI AATATT 1 cut(s) 476
SspMI CTAG 3 cut(s) 48, 384, 786
StyD4I CCNGG 2 cut(s) 201, 856
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 147, 685
TasI AATT 2 cut(s) 113, 396
TatI WGTACW 2 cut(s) 462, 744
TfiI GAWTC 5 cut(s) 34, 41, 149, 319, 379
Tru1I TTAA 2 cut(s) 213, 753
Tru9I TTAA 2 cut(s) 213, 753
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 6 cut(s) 96, 126, 428, 610, 647, 794
TspGWI ACGGA 2 cut(s) 39, 496
VpaK11BI GGWCC 1 cut(s) 776
XceI RCATGY 2 cut(s) 94, 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 3 cut(s) 48, 384, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.