pycom17g03610

Zinc finger AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011887
Physical Location & Seq
Forward (+)
2419684 .. 2420342
659 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGACAGGAGGAACAGAAGCTTTCCCGGATTTGGGAAGACACTGCCAGCTCTCCGATTGCCACCAGCTCGATTTCCTCCCCTTCCAATGTGACGGTTGTAGTAAGGTTTTCTGCGTTGAGCATCGGTCCTACAAGTCCCACGAATGCCCGAAATCGGACCACAACAGCAGAAAGGTGGTGGTTTGCGAAATCTGCTCGGCCTCCATAGAAACCACCGGCCGCGACGGCGAACAGGACCAGAAATTGCTGTTGGAGAAGCACGCCAAGTCCGGAAATTGCGACCCCAGAAAGAAGAAGAAACCAACCTGCCCGGTTCGGCGGTGTAAGGAGATTCTCACGTTTTCGAATACCAGCACCTGCAAGACTTGCCAGATCAAGGTTTGCCTCAAGCACCGGTTCCCGGCCGACCATGTTTGCAGGAAGCAGACGGCGGCACAGCCGTCGTTGGCGGGGAAGGCTGTGAGCTGGAACGACAAGTTCATGGCTGCTTTTGCTTCGAGGGAAGGGAAAGAATGTGGGAAGAGTGCGCGGGATTCCAAGTCTTCGGCTTCGAGAGCGCCTTCTGTTCGAGCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010200 GO:0010243 GO:0033993 GO:0042221 GO:0042493 GO:0050896 GO:0097305 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-AN1

Protein Analysis

193

Amino Acids

21.32

Weight (kDa)

9.13

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AN1 PF01428 15 - 52 5.7e-12 AN1-like Zinc finger
zf-AN1 PF01428 103 - 142 4.1e-08 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28210 AT3G28210
fragaria_vesca FvH4_6g50080
malus_domestica MD09G1039300.v1.1 MD17G1040300.v1.1
prunus_persica Prupe.3G278700_v2.0.a1
pyrus_communis pycom17g03610
rosa_chinensis RchiOBHm_Chr2g0170351
rosa_laevigata RLG00000021948
rosa_multiflora Rmu_sc0011601.1_g000011 Rmu_sc0012445.1_g000015
rosa_roxburghii Rroxscaffold_2G00081420
rosa_rugosa Rorug02G0548400
rosa_samantha Rh2AG619800 Rh2BG630700 Rh2CG600800 Rh2DG643600
rosa_wichuraiana Rw2G051340 Rw2G051350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 365
Acc36I ACCTGC 2 cut(s) 314, 365
AccII CGCG 2 cut(s) 222, 529
AccIII TCCGGA 1 cut(s) 269
AciI CCGC 5 cut(s) 220, 319, 431, 449, 529
AcoI YGGCCR 2 cut(s) 217, 402
AfiI CCNNNNNNNGG 5 cut(s) 31, 32, 154, 376, 400
AgeI ACCGGT 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 5 cut(s) 20, 49, 67, 465, 572
AluI AGCT 5 cut(s) 20, 49, 67, 465, 572
AlwNI CAGNNNCTG 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 269
AoxI GGCC 3 cut(s) 198, 217, 402
ApeKI GCWGC 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 393
AspLEI GCGC 2 cut(s) 529, 559
AspS9I GGNCC 3 cut(s) 126, 157, 235
AsuC2I CCSGG 3 cut(s) 26, 311, 401
AsuII TTCGAA 1 cut(s) 344
AvaII GGWCC 3 cut(s) 126, 157, 235
BbsI GAAGAC 2 cut(s) 43, 534
BbvI GCAGC 1 cut(s) 472
BceAI ACGGC 3 cut(s) 241, 424, 444
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BcnI CCSGG 3 cut(s) 26, 311, 401
BfoI RGCGCY 1 cut(s) 560
BfuAI ACCTGC 2 cut(s) 314, 365
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 3 cut(s) 220, 432, 486
BlsI GCNGC 3 cut(s) 221, 433, 487
Bme1390I CCNGG 3 cut(s) 26, 311, 401
Bme18I GGWCC 3 cut(s) 126, 157, 235
BmgT120I GGNCC 3 cut(s) 126, 157, 235
BmiI GGNNCC 1 cut(s) 398
BmrFI CCNGG 3 cut(s) 26, 311, 401
BmsI GCATC 1 cut(s) 130
BpiI GAAGAC 2 cut(s) 43, 534
Bpu14I TTCGAA 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 3 cut(s) 26, 311, 401
BsaWI WCCGGW 2 cut(s) 269, 393
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 32, 154, 376, 400
Bse118I RCCGGY 2 cut(s) 215, 393
BseAI TCCGGA 1 cut(s) 269
BseLI CCNNNNNNNGG 5 cut(s) 31, 32, 154, 376, 400
BseX3I CGGCCG 2 cut(s) 217, 402
BseXI GCAGC 1 cut(s) 472
Bsh1236I CGCG 2 cut(s) 222, 529
Bsh1285I CGRYCG 2 cut(s) 220, 405
BshFI GGCC 3 cut(s) 200, 219, 404
BshTI ACCGGT 1 cut(s) 393
BsiEI CGRYCG 2 cut(s) 220, 405
BsiSI CCGG 6 cut(s) 26, 216, 270, 311, 394, 401
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 5 cut(s) 31, 32, 154, 376, 400
BsmFI GGGAC 1 cut(s) 121
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 200, 219, 404
Bsp119I TTCGAA 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 269
Bsp143I GATC 1 cut(s) 372
BspACI CCGC 5 cut(s) 220, 319, 431, 449, 529
BspANI GGCC 3 cut(s) 200, 219, 404
BspEI TCCGGA 1 cut(s) 269
BspFNI CGCG 2 cut(s) 222, 529
BspLI GGNNCC 1 cut(s) 398
BspMI ACCTGC 2 cut(s) 314, 365
BspT104I TTCGAA 1 cut(s) 344
BsrFI RCCGGY 2 cut(s) 215, 393
BssAI RCCGGY 2 cut(s) 215, 393
BssMI GATC 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 47, 261
BstFNI CGCG 2 cut(s) 222, 529
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 2 cut(s) 529, 559
BstKTI GATC 1 cut(s) 375
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 2 cut(s) 220, 405
BstMWI GCNNNNNNNGC 6 cut(s) 192, 225, 366, 455, 491, 554
BstSCI CCNGG 3 cut(s) 24, 309, 399
BstUI CGCG 2 cut(s) 222, 529
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 2 cut(s) 43, 534
BstZI CGGCCG 2 cut(s) 217, 402
BsuRI GGCC 3 cut(s) 200, 219, 404
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
BveI ACCTGC 2 cut(s) 314, 365
Cac8I GCNNGC 2 cut(s) 47, 261
CaiI CAGNNNCTG 1 cut(s) 357
CfoI GCGC 2 cut(s) 529, 559
Cfr10I RCCGGY 2 cut(s) 215, 393
Cfr13I GGNCC 3 cut(s) 126, 157, 235
CspAI ACCGGT 1 cut(s) 393
CviAII CATG 2 cut(s) 410, 481
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
EaeI YGGCCR 2 cut(s) 217, 402
EagI CGGCCG 2 cut(s) 217, 402
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
EclXI CGGCCG 2 cut(s) 217, 402
Eco47I GGWCC 3 cut(s) 126, 157, 235
Eco52I CGGCCG 2 cut(s) 217, 402
FaeI CATG 2 cut(s) 413, 484
FaiI YATR 3 cut(s) 206, 411, 482
FaqI GGGAC 1 cut(s) 121
FatI CATG 2 cut(s) 409, 480
FauI CCCGC 2 cut(s) 442, 522
Fnu4HI GCNGC 3 cut(s) 220, 432, 486
Fsp4HI GCNGC 3 cut(s) 220, 432, 486
GlaI GCGC 2 cut(s) 528, 558
GluI GCNGC 3 cut(s) 220, 432, 486
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 3 cut(s) 200, 219, 404
HapII CCGG 6 cut(s) 26, 216, 270, 311, 394, 401
HhaI GCGC 2 cut(s) 529, 559
Hin1II CATG 2 cut(s) 413, 484
Hin6I GCGC 2 cut(s) 527, 557
HinP1I GCGC 2 cut(s) 527, 557
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 331, 533
HpaII CCGG 6 cut(s) 26, 216, 270, 311, 394, 401
Hpy188I TCNGA 2 cut(s) 55, 157
Hpy188III TCNNGA 2 cut(s) 270, 552
Hpy99I CGWCG 2 cut(s) 227, 445
HpyAV CCTTC 4 cut(s) 91, 448, 497, 570
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 2 cut(s) 360, 417
HpyF10VI GCNNNNNNNGC 6 cut(s) 192, 225, 366, 455, 491, 554
HpySE526I ACGT 1 cut(s) 338
Hsp92II CATG 2 cut(s) 413, 484
HspAI GCGC 2 cut(s) 527, 557
Kpn2I TCCGGA 1 cut(s) 269
Kzo9I GATC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 1 cut(s) 130
MaeII ACGT 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 89
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MboII GAAGA 5 cut(s) 48, 304, 307, 532, 534
MluCI AATT 2 cut(s) 242, 274
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 4 cut(s) 86, 211, 395, 492
MroI TCCGGA 1 cut(s) 269
MspI CCGG 6 cut(s) 26, 216, 270, 311, 394, 401
MspR9I CCNGG 3 cut(s) 26, 311, 401
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 2 cut(s) 222, 529
MwoI GCNNNNNNNGC 6 cut(s) 192, 225, 366, 455, 491, 554
NciI CCSGG 3 cut(s) 26, 311, 401
NdeII GATC 1 cut(s) 372
NlaIII CATG 2 cut(s) 413, 484
NlaIV GGNNCC 1 cut(s) 398
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 89
NspV TTCGAA 1 cut(s) 344
PaqCI CACCTGC 1 cut(s) 365
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 331, 533
PfoI TCCNGGA 1 cut(s) 24
PinAI ACCGGT 1 cut(s) 393
PkrI GCNGC 3 cut(s) 221, 433, 487
PspN4I GGNNCC 1 cut(s) 398
PspPI GGNCC 3 cut(s) 126, 157, 235
PstNI CAGNNNCTG 1 cut(s) 357
SatI GCNGC 3 cut(s) 220, 432, 486
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 3 cut(s) 126, 157, 235
ScrFI CCNGG 3 cut(s) 26, 311, 401
SfaNI GCATC 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 344
SinI GGWCC 3 cut(s) 126, 157, 235
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 242, 274
SsiI CCGC 5 cut(s) 220, 319, 431, 449, 529
StyD4I CCNGG 3 cut(s) 24, 309, 399
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 1 cut(s) 341
TaqI TCGA 5 cut(s) 69, 344, 497, 551, 568
TaqII GACCGA 1 cut(s) 114
TasI AATT 2 cut(s) 242, 274
TauI GCSGC 2 cut(s) 222, 434
TfiI GAWTC 2 cut(s) 331, 533
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 1 cut(s) 469
TspRI CASTG 1 cut(s) 47
VpaK11BI GGWCC 3 cut(s) 126, 157, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.