pycom17g07480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009703
Physical Location & Seq
Forward (+)
5453233 .. 5457126
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
ATGAAATTAGGAGTCTCAAATCTCAAACCCTCCCCAAATACCAAACCAAACCCAAAAGAACAAAAATTCAAAGACAATGTTCCTCTTGTTCCTCCCACACAACAACAACTAACAAAAATCTCAGACAGGCAGTTTTGGTGGTTTTGTTTGTTGGGGGTCCATCATCATCATCTTCTTCTTATTCTCGCTCCTCTTCTAATGGTAGTTGTCCAAGCTTCCAAGCTCAGCCTCCCAAACCCTTCTCTCACCTCCCCTCCCCAAATAACTTCCCTCCAGTTTGAACCCCATTCTCTCTCTCTAGCCCTCATGCACTCTGACTCCACTTTTTCTCTCTACCCCTCTCTCTCCTCCCCTCTTTCCCTCTCCTCTCTCCCTCCTCCCCAAACCCTAATTGGCCCTCCCTCCTCTTCCTCCACCTTCCTCCTCCTCCAAAACCCAGACCCTAACCCCAACACCCGGACCCTCTTCATTGCCTGCGGCCCTCACAGAGGTGGGTCCCAGGTCCTGCTCAGGTTCTACATTCTGCAGAATCAGAAACAATTCGTGAGGGCTCAAGTCGTTTGCACCCAGAAAGGGCTAAAATTTGATGGGAAATTGGGGGTTTTGGTTGATGCCCATCATGGGGTTTCGCTTAAGCTTGCCGGGTCGGTCAATTTCTTCGCCATGTACTCCGTTTCGAGCTCAAAGATATCAGTTTTTGCTGTGAAAGCGATTGGTGATGGCGACAATGGTGATGATGGGGTGGTTGTGAAGTTGATGAGGTGTGCTGTGATTGAGTGTTGCAAGCTGGTTTGGTCGATTAGCATTTCGTTTGGGGTCTTGATCTTAGGAGAAGATAATGGAGTTAGGGTTTTTAATTTGAGGCAGCTTGTTAAGGGGCGGGTTCGAAAGGCCAAGACTTTGAGTTCGAGTTTGAAAAATGAGGGCCGAAATTTGGGTTTTCCTAATGGGGTGGTTGGTGATCATGTGCATAGTGATTTGGGGGATTGTGGAAATAAGCAAGGAGGCGACAAATGTGGTAGTGTTGAGGGGAGTTCGGAGATGACTTGCAGTGGTAACTTGTGTGGGAAGAATGATAGGAATTATGTTTCTGTCAAGCAGAGGTCTGTGAAACTGAAACAAGACTCTTGTGAAGAGGGAGTGTTTTTTGTGGAATTCAAAGGAAAGGAGTTTGAAACCTCAAAATCGAAAAGAGTGATACCAGCTAAGGCACTTTCTATTGAAGCACTGTCCCCCAACAAATTTCTAATCTTGGATTCTGATGGAGGTTTACACATTTTGCACCTCTCTAGTCCTATAATTGGATTAGAAATAACTTCTTGTGTGCAGCAGTTGCCTCACATTATGAAAGTGCAAAAGCTGGCTGTTCTTCCTGATATAGATTCAAGAACTCAATCTGTGTGGGCATCAGATGGATACAATTCTGTGCAGATGTTGTTGGCATCTCACATGGATATTGCGGAAAATGAGAAAAATAACAGCGAGGAAAAACTGATTCATGTCTCAGTTCTTCTCACAATTTTTGCCAGCGAAAGAATTCAAGATCTTATTCCTATGGCTGCAAATGCTATTTTGATTCTTGGACAAGGAAGCTTGTATACGTATGCGATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

539

Amino Acids

58.57

Weight (kDa)

8.93

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1598
AciI CCGC 3 cut(s) 477, 880, 1460
AcsI RAATTY 6 cut(s) 65, 581, 931, 1154, 1241, 1536
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 7 cut(s) 456, 488, 573, 621, 622, 934, 1301
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 8 cut(s) 70, 281, 916, 1159, 1175, 1223, 1386, 1541
AjnI CCWGG 1 cut(s) 498
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 9 cut(s) 215, 223, 637, 681, 787, 868, 1205, 1360, 1593
AluI AGCT 9 cut(s) 215, 223, 637, 681, 787, 868, 1205, 1360, 1593
Alw21I GWGCWC 1 cut(s) 683
Alw26I GTCTC 2 cut(s) 19, 1507
AlwNI CAGNNNCTG 1 cut(s) 505
AoxI GGCC 4 cut(s) 394, 478, 891, 925
ApeKI GCWGC 3 cut(s) 865, 1327, 1559
ApoI RAATTY 6 cut(s) 65, 581, 931, 1154, 1241, 1536
ArsI GACNNNNNNTTYG 2 cut(s) 889, 921
Asp700I GAANNNNTTC 2 cut(s) 539, 1536
AspS9I GGNCC 7 cut(s) 157, 395, 459, 479, 495, 502, 925
AsuC2I CCSGG 2 cut(s) 457, 643
AsuHPI GGTGA 4 cut(s) 238, 728, 743, 971
AsuII TTCGAA 1 cut(s) 886
AvaII GGWCC 4 cut(s) 157, 459, 495, 502
BanII GRGCYC 2 cut(s) 553, 683
BarI GAAGNNNNNNTAC 2 cut(s) 1582, 1614
Bbv12I GWGCWC 1 cut(s) 683
BbvI GCAGC 3 cut(s) 877, 1339, 1546
BccI CCATC 7 cut(s) 168, 581, 624, 713, 731, 1256, 1406
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 1409
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 2 cut(s) 457, 643
BcoDI GTCTC 2 cut(s) 19, 1507
BfaI CTAG 2 cut(s) 299, 1290
BfmI CTRYAG 1 cut(s) 524
BfrI CTTAAG 1 cut(s) 632
BfuI GTATCC 1 cut(s) 1409
BglII AGATCT 1 cut(s) 1543
BisI GCNGC 4 cut(s) 478, 866, 1328, 1560
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 4 cut(s) 479, 867, 1329, 1561
Bme1390I CCNGG 3 cut(s) 457, 500, 643
Bme18I GGWCC 4 cut(s) 157, 459, 495, 502
BmgT120I GGNCC 7 cut(s) 157, 395, 459, 479, 495, 502, 925
BmiI GGNNCC 4 cut(s) 158, 461, 496, 497
BmrFI CCNGG 3 cut(s) 457, 500, 643
BmsI GCATC 3 cut(s) 601, 1415, 1451
BpmI CTGGAG 1 cut(s) 257
Bpu10I CCTNAGC 2 cut(s) 509, 1206
Bpu1102I GCTNAGC 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 537
BpuMI CCSGG 2 cut(s) 457, 643
BsaAI YACGTR 1 cut(s) 1602
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 498
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 302, 332, 834, 864
Bsc4I CCNNNNNNNGG 7 cut(s) 456, 488, 573, 621, 622, 934, 1301
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 498
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 7 cut(s) 456, 488, 573, 621, 622, 934, 1301
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 4 cut(s) 135, 238, 523, 1518
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 7 cut(s) 180, 337, 355, 366, 394, 413, 416
BseXI GCAGC 3 cut(s) 877, 1339, 1546
BsgI GTGCAG 2 cut(s) 1346, 1448
BshFI GGCC 4 cut(s) 396, 480, 893, 927
BsiHKAI GWGCWC 1 cut(s) 683
BsiSI CCGG 2 cut(s) 457, 642
BslFI GGGAC 2 cut(s) 481, 1216
BslI CCNNNNNNNGG 7 cut(s) 456, 488, 573, 621, 622, 934, 1301
BsmAI GTCTC 2 cut(s) 19, 1507
BsmFI GGGAC 2 cut(s) 481, 1216
BsnI GGCC 4 cut(s) 396, 480, 893, 927
Bsp119I TTCGAA 1 cut(s) 886
Bsp1286I GDGCHC 2 cut(s) 553, 683
Bsp143I GATC 3 cut(s) 822, 961, 1543
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 3 cut(s) 477, 880, 1460
BspANI GGCC 4 cut(s) 396, 480, 893, 927
BspCNI CTCAG 4 cut(s) 134, 237, 522, 1517
BspLI GGNNCC 4 cut(s) 158, 461, 496, 497
BspMAI CTGCAG 1 cut(s) 528
BspT104I TTCGAA 1 cut(s) 886
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 822, 961, 1543
BssNAI GTATAC 1 cut(s) 1599
Bst1107I GTATAC 1 cut(s) 1599
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 1230
Bst6I CTCTTC 4 cut(s) 198, 412, 470, 1128
BstAFI CTTAAG 1 cut(s) 632
BstAPI GCANNNNNTGC 1 cut(s) 1333
BstBAI YACGTR 1 cut(s) 1602
BstBI TTCGAA 1 cut(s) 886
BstC8I GCNNGC 5 cut(s) 475, 639, 785, 1362, 1528
BstDEI CTNAG 6 cut(s) 121, 224, 509, 826, 1206, 1504
BstENI CCTNNNNNAGG 1 cut(s) 486
BstKTI GATC 3 cut(s) 825, 964, 1546
BstMAI GTCTC 2 cut(s) 19, 1507
BstMBI GATC 3 cut(s) 822, 961, 1543
BstMWI GCNNNNNNNGC 3 cut(s) 707, 1333, 1565
BstNI CCWGG 1 cut(s) 500
BstSCI CCNGG 3 cut(s) 455, 498, 641
BstSFI CTRYAG 1 cut(s) 524
BstSNI TACGTA 1 cut(s) 1602
BstV1I GCAGC 3 cut(s) 877, 1339, 1546
BstX2I RGATCY 1 cut(s) 1543
BstYI RGATCY 1 cut(s) 1543
BstZ17I GTATAC 1 cut(s) 1599
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 4 cut(s) 396, 480, 893, 927
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 2 cut(s) 1057, 1226
Cac8I GCNNGC 5 cut(s) 475, 639, 785, 1362, 1528
CaiI CAGNNNCTG 1 cut(s) 505
Cfr13I GGNCC 7 cut(s) 157, 395, 459, 479, 495, 502, 925
Csp6I GTAC 1 cut(s) 667
CspCI CAANNNNNGTGG 2 cut(s) 1382, 1417
CviAII CATG 6 cut(s) 307, 620, 664, 965, 1450, 1499
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 6 cut(s) 121, 224, 509, 826, 1206, 1504
DpnI GATC 3 cut(s) 824, 963, 1545
DpnII GATC 3 cut(s) 822, 961, 1543
Eam1104I CTCTTC 4 cut(s) 198, 412, 470, 1128
EarI CTCTTC 4 cut(s) 198, 412, 470, 1128
Ecl136II GAGCTC 1 cut(s) 681
Eco105I TACGTA 1 cut(s) 1602
Eco24I GRGCYC 2 cut(s) 553, 683
Eco32I GATATC 1 cut(s) 690
Eco47I GGWCC 4 cut(s) 157, 459, 495, 502
Eco53kI GAGCTC 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 681
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 2 cut(s) 495, 502
EcoRI GAATTC 2 cut(s) 1154, 1536
EcoRII CCWGG 1 cut(s) 498
EcoRV GATATC 1 cut(s) 690
EcoT38I GRGCYC 2 cut(s) 553, 683
FaeI CATG 6 cut(s) 310, 623, 667, 968, 1453, 1502
FaqI GGGAC 2 cut(s) 481, 1216
FatI CATG 6 cut(s) 306, 619, 663, 964, 1449, 1498
FauI CCCGC 1 cut(s) 873
FbaI TGATCA 1 cut(s) 961
FblI GTMKAC 1 cut(s) 1598
Fnu4HI GCNGC 4 cut(s) 478, 866, 1328, 1560
FriOI GRGCYC 2 cut(s) 553, 683
Fsp4HI GCNGC 4 cut(s) 478, 866, 1328, 1560
FspBI CTAG 2 cut(s) 299, 1290
GluI GCNGC 4 cut(s) 478, 866, 1328, 1560
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 4 cut(s) 396, 480, 893, 927
HapII CCGG 2 cut(s) 457, 642
Hin1II CATG 6 cut(s) 310, 623, 667, 968, 1453, 1502
HindIII AAGCTT 3 cut(s) 213, 635, 1591
HinfI GANTC 8 cut(s) 12, 317, 529, 1124, 1256, 1382, 1495, 1576
HpaII CCGG 2 cut(s) 457, 642
HphI GGTGA 4 cut(s) 238, 728, 743, 971
Hpy166II GTNNAC 2 cut(s) 1271, 1599
Hpy188I TCNGA 6 cut(s) 124, 316, 534, 1039, 1261, 1411
Hpy188III TCNNGA 6 cut(s) 544, 820, 1373, 1386, 1541, 1614
Hpy8I GTNNAC 2 cut(s) 1271, 1599
HpyAV CCTTC 2 cut(s) 249, 427
HpyCH4III ACNGT 1 cut(s) 1230
HpyCH4IV ACGT 1 cut(s) 1601
HpyF10VI GCNNNNNNNGC 3 cut(s) 707, 1333, 1565
HpyF3I CTNAG 6 cut(s) 121, 224, 509, 826, 1206, 1504
HpySE526I ACGT 1 cut(s) 1601
Hsp92II CATG 6 cut(s) 310, 623, 667, 968, 1453, 1502
KflI GGGWCCC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 3 cut(s) 822, 961, 1543
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 3 cut(s) 877, 1339, 1546
LweI GCATC 3 cut(s) 601, 1415, 1451
MaeI CTAG 2 cut(s) 299, 1290
MaeII ACGT 1 cut(s) 1601
MaeIII GTNAC 1 cut(s) 1055
MalI GATC 3 cut(s) 824, 963, 1545
MboI GATC 3 cut(s) 822, 961, 1543
MflI RGATCY 1 cut(s) 1543
MhlI GDGCHC 2 cut(s) 553, 683
MlyI GAGTC 3 cut(s) 21, 311, 1118
MroXI GAANNNNTTC 2 cut(s) 539, 1536
MseI TTAA 3 cut(s) 633, 855, 873
MslI CAYNNNNRTG 1 cut(s) 489
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 2 cut(s) 457, 642
MspR9I CCNGG 3 cut(s) 457, 500, 643
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 707, 1333, 1565
NciI CCSGG 2 cut(s) 457, 643
NdeII GATC 3 cut(s) 822, 961, 1543
NlaIII CATG 6 cut(s) 310, 623, 667, 968, 1453, 1502
NlaIV GGNNCC 4 cut(s) 158, 461, 496, 497
NspV TTCGAA 1 cut(s) 886
OliI CACNNNNGTG 1 cut(s) 489
PdmI GAANNNNTTC 2 cut(s) 539, 1536
PfeI GAWTC 5 cut(s) 529, 1256, 1382, 1495, 1576
PkrI GCNGC 4 cut(s) 479, 867, 1329, 1561
PleI GAGTC 3 cut(s) 20, 311, 1118
PpsI GAGTC 3 cut(s) 20, 311, 1118
Ppu21I YACGTR 1 cut(s) 1602
PpuMI RGGWCCY 2 cut(s) 495, 502
Psp124BI GAGCTC 1 cut(s) 683
Psp5II RGGWCCY 2 cut(s) 495, 502
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 4 cut(s) 158, 461, 496, 497
PspPI GGNCC 7 cut(s) 157, 395, 459, 479, 495, 502, 925
PspPPI RGGWCCY 2 cut(s) 495, 502
PstI CTGCAG 1 cut(s) 528
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 1543
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 1 cut(s) 683
SaqAI TTAA 3 cut(s) 633, 855, 873
SatI GCNGC 4 cut(s) 478, 866, 1328, 1560
Sau3AI GATC 3 cut(s) 822, 961, 1543
Sau96I GGNCC 7 cut(s) 157, 395, 459, 479, 495, 502, 925
SchI GAGTC 3 cut(s) 21, 311, 1118
ScrFI CCNGG 3 cut(s) 457, 500, 643
SduI GDGCHC 2 cut(s) 553, 683
SfaNI GCATC 3 cut(s) 601, 1415, 1451
SfcI CTRYAG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 886
SinI GGWCC 4 cut(s) 157, 459, 495, 502
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 552, 632
SmoI CTYRAG 2 cut(s) 552, 632
SnaBI TACGTA 1 cut(s) 1602
SsiI CCGC 3 cut(s) 477, 880, 1460
SspMI CTAG 2 cut(s) 299, 1290
SstI GAGCTC 1 cut(s) 683
StyD4I CCNGG 3 cut(s) 455, 498, 641
TaaI ACNGT 1 cut(s) 1230
TaiI ACGT 1 cut(s) 1604
TaqI TCGA 5 cut(s) 677, 797, 886, 908, 1187
TaqII GACCGA 1 cut(s) 637
TatI WGTACW 1 cut(s) 666
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 5 cut(s) 529, 1256, 1382, 1495, 1576
Tru1I TTAA 3 cut(s) 633, 855, 873
Tru9I TTAA 3 cut(s) 633, 855, 873
TscAI CASTG 2 cut(s) 1057, 1233
TseI GCWGC 3 cut(s) 865, 1327, 1559
TspDTI ATGAA 4 cut(s) 17, 457, 1361, 1487
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 2 cut(s) 1057, 1233
Vha464I CTTAAG 1 cut(s) 632
VpaK11BI GGWCC 4 cut(s) 157, 459, 495, 502
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 6 cut(s) 65, 581, 931, 1154, 1241, 1536
XcmI CCANNNNNNNNNTGG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 1598
XmnI GAANNNNTTC 2 cut(s) 539, 1536
XspI CTAG 2 cut(s) 299, 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.