pycom17g07500

Protein of unknown function (DUF2854)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009598
Physical Location & Seq
Reverse (-)
5466283 .. 5469302
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGGCAACGCAAGCACTATCAACTCCAACTGCAATTGCAAGAGGAAGCAGCAGCTTCTGCCGCCACCATGACGTCAACCGCCACCACTTTCTCTCTCTGCGTGACCATAACACGGCCTCATCATCATCAAGGCCCATCACCAGTTTCCTCATCCACTTGGATCACATTGGATTCAGAGCCAACCAACTCTCGAAGTCCAAGCCCAAACTGGCCATCCCGAGAGCCGCCGACTCCTCTACCCAGCCTTCCACCCTCCCCTCCACCTCCACCGCCGGCAAAACCATCGTTCCCGACGACCAGTTCTCCCTCGCTAAGGTTTCATTTGGGGTTATTGGTCTTGGTGTTGGGGTTTCACTCTTGTCGTATGGTTTCGGGGCGTACTTTAATATCCTTCCCGGGTCTGAATGGTCAGCATTGATGCTCACATATGGCTTCCCTCTTGCAATCATTGGTATGGCATTCAAGTATGCTGAACTAAAGCCGGTGCCATGCTTGACTTATTCGGACGCCCTGCAATTAAGGGAAACAAGTGCCACGCCGATCCTTACACAGGTCAGGAATGATGTTACAAGGTATCGCTATGGAGATGAACAGCATTTGGATGAGGCATTGAAACGAATCTTCCAGTATGGTCAGGGTGGAGGAATTGCTCGGAGGAGTGCACCTACTCTCCAAAGTATTCGTGAAGAGGTCACCGGAGATGGTAGATACTCATTAGTCCTGGTGTTTGAGGCAAAAGCTTTGCAGTTATCAGATTTTGAACAACGACAGGCCAAGTTTGCTTCATTCTTCGGACCAGGAATCACAGCTGAAGTTGGCAAGGGAGAAAATAATCTATACGAAGTCCGACTTATTTCCAACTCCAACGCTAAACCGTCGGCATCACCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2854

Protein Analysis

298

Amino Acids

32.3

Weight (kDa)

9.3

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2854 PF11016 122 - 272 2e-47 Protein of unknown function (DUF2854)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 484
AciI CCGC 4 cut(s) 61, 79, 225, 270
AclWI GGATC 2 cut(s) 168, 535
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 831
AcyI GRCGYC 2 cut(s) 72, 507
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 3 cut(s) 112, 313, 550
AgsI TTSAA 3 cut(s) 463, 613, 761
AjnI CCWGG 2 cut(s) 720, 796
AluBI AGCT 3 cut(s) 54, 740, 809
AluI AGCT 3 cut(s) 54, 740, 809
Alw21I GWGCWC 1 cut(s) 664
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 2 cut(s) 168, 535
AlwNI CAGNNNCTG 1 cut(s) 57
Ama87I CYCGRG 2 cut(s) 217, 395
AoxI GGCC 4 cut(s) 114, 131, 210, 771
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 2 cut(s) 48, 51
AspS9I GGNCC 2 cut(s) 132, 794
AsuC2I CCSGG 2 cut(s) 396, 397
AsuHPI GGTGA 3 cut(s) 130, 685, 876
AvaI CYCGRG 2 cut(s) 217, 395
AvaII GGWCC 1 cut(s) 794
BaeGI GKGCMC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 212
BanI GGYRCC 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 2 cut(s) 60, 63
BccI CCATC 4 cut(s) 143, 221, 290, 695
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 2 cut(s) 722, 798
BcnI CCSGG 2 cut(s) 396, 397
BisI GCNGC 4 cut(s) 49, 52, 61, 225
BlsI GCNGC 4 cut(s) 50, 53, 62, 226
Bme1390I CCNGG 4 cut(s) 396, 397, 722, 798
Bme18I GGWCC 1 cut(s) 794
BmeT110I CYCGRG 2 cut(s) 217, 395
BmgT120I GGNCC 2 cut(s) 132, 794
BmiI GGNNCC 1 cut(s) 486
BmrFI CCNGG 4 cut(s) 396, 397, 722, 798
BmsI GCATC 2 cut(s) 408, 890
Bpu10I CCTNAGC 1 cut(s) 312
BpuMI CCSGG 2 cut(s) 396, 397
BsaHI GRCGYC 2 cut(s) 72, 507
BsaJI CCNNGG 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 695
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 654, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 313, 550
Bse118I RCCGGY 2 cut(s) 272, 481
Bse1I ACTGG 4 cut(s) 141, 213, 298, 625
BseBI CCWGG 2 cut(s) 722, 798
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 3 cut(s) 150, 213, 607
BseLI CCNNNNNNNGG 3 cut(s) 112, 313, 550
BseNI ACTGG 4 cut(s) 141, 213, 298, 625
BseRI GAGGAG 2 cut(s) 223, 670
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 2 cut(s) 60, 63
BseYI CCCAGC 1 cut(s) 240
BshFI GGCC 4 cut(s) 116, 133, 212, 773
BshNI GGYRCC 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 2 cut(s) 217, 395
BsiSI CCGG 4 cut(s) 273, 396, 482, 696
BslI CCNNNNNNNGG 3 cut(s) 112, 313, 550
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 4 cut(s) 116, 133, 212, 773
BsoBI CYCGRG 2 cut(s) 217, 395
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 160, 540
BspACI CCGC 4 cut(s) 61, 79, 225, 270
BspANI GGCC 4 cut(s) 116, 133, 212, 773
BspLI GGNNCC 1 cut(s) 486
BspPI GGATC 2 cut(s) 168, 535
BspT107I GGYRCC 1 cut(s) 484
BsrFI RCCGGY 2 cut(s) 272, 481
BsrI ACTGG 4 cut(s) 141, 213, 298, 625
BssAI RCCGGY 2 cut(s) 272, 481
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 2 cut(s) 160, 540
BssNI GRCGYC 2 cut(s) 72, 507
Bst2UI CCWGG 2 cut(s) 722, 798
Bst4CI ACNGT 2 cut(s) 876, 888
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 2 cut(s) 72, 507
BstAPI GCANNNNNTGC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 12, 274
BstDEI CTNAG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 691
BstENI CCTNNNNNAGG 2 cut(s) 311, 548
BstF5I GGATG 3 cut(s) 150, 213, 607
BstKTI GATC 2 cut(s) 163, 543
BstMBI GATC 2 cut(s) 160, 540
BstMWI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 779
BstNI CCWGG 2 cut(s) 722, 798
BstPI GGTNACC 1 cut(s) 691
BstSCI CCNGG 4 cut(s) 394, 395, 720, 796
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 2 cut(s) 60, 63
BsuRI GGCC 4 cut(s) 116, 133, 212, 773
BtsCI GGATG 3 cut(s) 150, 213, 607
Cac8I GCNNGC 2 cut(s) 12, 274
CaiI CAGNNNCTG 1 cut(s) 57
Cfr10I RCCGGY 2 cut(s) 272, 481
Cfr13I GGNCC 2 cut(s) 132, 794
Cfr9I CCCGGG 1 cut(s) 395
CseI GACGC 1 cut(s) 515
Csp6I GTAC 1 cut(s) 379
CviAII CATG 2 cut(s) 68, 489
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 312
DpnI GATC 2 cut(s) 162, 542
DpnII GATC 2 cut(s) 160, 540
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 831
Eco88I CYCGRG 2 cut(s) 217, 395
Eco91I GGTNACC 1 cut(s) 691
EcoNI CCTNNNNNAGG 2 cut(s) 311, 548
EcoO65I GGTNACC 1 cut(s) 691
EcoRII CCWGG 2 cut(s) 720, 796
FaeI CATG 2 cut(s) 71, 492
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FatI CATG 2 cut(s) 67, 488
FauNDI CATATG 1 cut(s) 427
Fnu4HI GCNGC 4 cut(s) 49, 52, 61, 225
FokI GGATG 3 cut(s) 137, 200, 614
Fsp4HI GCNGC 4 cut(s) 49, 52, 61, 225
GluI GCNGC 4 cut(s) 49, 52, 61, 225
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 4 cut(s) 116, 133, 212, 773
HapII CCGG 4 cut(s) 273, 396, 482, 696
HgaI GACGC 1 cut(s) 515
Hin1I GRCGYC 2 cut(s) 72, 507
Hin1II CATG 2 cut(s) 71, 492
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 4 cut(s) 171, 230, 618, 801
HpaII CCGG 4 cut(s) 273, 396, 482, 696
HphI GGTGA 3 cut(s) 130, 685, 876
Hpy166II GTNNAC 2 cut(s) 76, 662
Hpy188I TCNGA 7 cut(s) 176, 403, 505, 654, 754, 794, 848
Hpy188III TCNNGA 5 cut(s) 190, 217, 290, 556, 683
Hpy8I GTNNAC 2 cut(s) 76, 662
Hpy99I CGWCG 2 cut(s) 296, 880
HpyAV CCTTC 2 cut(s) 255, 401
HpyCH4III ACNGT 2 cut(s) 876, 888
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 6 cut(s) 32, 38, 443, 514, 662, 745
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 779
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 72, 507
Hsp92II CATG 2 cut(s) 71, 492
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 160, 540
Lsp1109I GCAGC 2 cut(s) 60, 63
LweI GCATC 2 cut(s) 408, 890
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 3 cut(s) 101, 565, 691
MalI GATC 2 cut(s) 162, 542
MboI GATC 2 cut(s) 160, 540
MboII GAAGA 3 cut(s) 613, 698, 781
MfeI CAATTG 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 3 cut(s) 33, 515, 645
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 4 cut(s) 50, 871, 882, 888
Mox20I TGGCCA 1 cut(s) 212
MroNI GCCGGC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 212
MseI TTAA 2 cut(s) 384, 518
MslI CAYNNNNRTG 2 cut(s) 452, 600
Msp20I TGGCCA 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 4 cut(s) 273, 396, 482, 696
MspR9I CCNGG 4 cut(s) 396, 397, 722, 798
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 2 cut(s) 722, 798
MwoI GCNNNNNNNGC 4 cut(s) 11, 57, 60, 779
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 396, 397
NdeI CATATG 1 cut(s) 427
NdeII GATC 2 cut(s) 160, 540
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 2 cut(s) 71, 492
NlaIV GGNNCC 1 cut(s) 486
NmuCI GTSAC 2 cut(s) 101, 691
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 884
PctI GAATGC 1 cut(s) 458
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 3 cut(s) 171, 618, 801
PkrI GCNGC 4 cut(s) 50, 53, 62, 226
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 720, 796
PspEI GGTNACC 1 cut(s) 691
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 2 cut(s) 720, 796
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 2 cut(s) 132, 794
PstNI CAGNNNCTG 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 2 cut(s) 452, 600
SaqAI TTAA 2 cut(s) 384, 518
SatI GCNGC 4 cut(s) 49, 52, 61, 225
Sau3AI GATC 2 cut(s) 160, 540
Sau96I GGNCC 2 cut(s) 132, 794
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 4 cut(s) 396, 397, 722, 798
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 2 cut(s) 408, 890
SinI GGWCC 1 cut(s) 794
SmaI CCCGGG 1 cut(s) 397
SmiMI CAYNNNNRTG 2 cut(s) 452, 600
Sse9I AATT 3 cut(s) 33, 515, 645
SsiI CCGC 4 cut(s) 61, 79, 225, 270
StyD4I CCNGG 4 cut(s) 394, 395, 720, 796
TaaI ACNGT 2 cut(s) 876, 888
TaiI ACGT 1 cut(s) 75
TaqI TCGA 1 cut(s) 191
TasI AATT 3 cut(s) 33, 515, 645
TauI GCSGC 2 cut(s) 63, 227
TfiI GAWTC 3 cut(s) 171, 618, 801
Tru1I TTAA 2 cut(s) 384, 518
Tru9I TTAA 2 cut(s) 384, 518
TseFI GTSAC 2 cut(s) 101, 691
TseI GCWGC 2 cut(s) 48, 51
Tsp45I GTSAC 2 cut(s) 101, 691
TspDTI ATGAA 3 cut(s) 309, 603, 774
TspMI CCCGGG 1 cut(s) 395
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 794
XagI CCTNNNNNAGG 2 cut(s) 311, 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmaI CCCGGG 1 cut(s) 395
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.