pycom17g09450

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007174
Physical Location & Seq
Reverse (-)
7210622 .. 7213787
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 882 bp
ATGCTGACAACTGTCGCTGCTGGTAGTTTGCGATCAGCAGCTTCCCGAGCCTTCTTCTTCTCCAAAGCTCTCCCAAACTCCGCCCCCACTTCCTTCTCACTCGCACGGACGCTCTCTCCCGCCGCCGTTTCTCGCTCCTCCTTCCGCGCCATGGCCTCCGATTCTGCTGCCCCCTTCAACAAAATCCAAATCCAAAGAGACGACACCACATTTGATGCATATGTTGTTGGCAAAGACGGCGCTCCTGGGATTATTGTGCTTCAAGAGTGGTGGGGTGTGGATTATGAAATTAAGAACCATGCTGTCAAGATTTCTCAACTTGCTCCTGGATTCAAAGCACTTATCCCAGACTTGTATCGGGGGAAGGTTGGTTTAGATGTTGCAGAAGCTCAACATTTGATGGACGGTCTTGATTGGCAAGGCGCTGTAAAGGATATCCAAGCTTCTGTTAATTGGCTGAAAGCAAATGGTTCGAAGAAGGTGGGTGTTACTGGATTTTGCATGGGTGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCTGAAGTTGATGCTGTTGTTGCATTTTATGGTGTGCCGTCATCAGAGCTTGCAGACCCTGCCAAAGCTAAGGCTCCTGTTCAGGCTCATTTTGGAGAGCTTGATAGTTTCGTTGGCTTTTCAGACGTGACGGCTGCCAAGTCTTTGGAGGAGAAGCTGAAAGCATCAGGAATCCCTTACGAGGTGCACATTTATCCCGGCAATACCCATGCGTTCATGAATAGGTCTCCAGAAGCTGTGAAGAGGAGGAAGGGCATGGGATTGGCTGATGAGGATGAAGCAGCATGTCAGCTGGCTTGGTCTCGCTTCCAATCATGGATGTCTTGTTATTTGCTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

294

Amino Acids

31.34

Weight (kDa)

6.96

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 71 - 290 2.6e-58 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 4 cut(s) 81, 120, 123, 145
AcuI CTGAAG 1 cut(s) 564
AfiI CCNNNNNNNGG 2 cut(s) 151, 721
AgsI TTSAA 3 cut(s) 178, 263, 334
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 2 cut(s) 244, 325
Alw21I GWGCWC 2 cut(s) 514, 729
Alw26I GTCTC 3 cut(s) 192, 771, 846
Alw44I GTGCAC 1 cut(s) 725
AlwNI CAGNNNCTG 2 cut(s) 599, 776
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 1 cut(s) 153
ApaLI GTGCAC 1 cut(s) 725
ApeKI GCWGC 5 cut(s) 17, 38, 167, 674, 821
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 3 cut(s) 149, 242, 425
AspS9I GGNCC 1 cut(s) 538
AsuC2I CCSGG 1 cut(s) 738
AsuII TTCGAA 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 729
Bbv12I GWGCWC 2 cut(s) 514, 729
BbvI GCAGC 5 cut(s) 4, 50, 154, 661, 833
BccI CCATC 1 cut(s) 394
BceAI ACGGC 4 cut(s) 110, 253, 562, 687
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 453, 487
BciT130I CCWGG 2 cut(s) 246, 327
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 3 cut(s) 192, 771, 846
BfoI RGCGCY 2 cut(s) 243, 426
BisI GCNGC 6 cut(s) 18, 39, 123, 168, 675, 822
BlsI GCNGC 6 cut(s) 19, 40, 124, 169, 676, 823
Bme1390I CCNGG 3 cut(s) 246, 327, 738
Bme18I GGWCC 1 cut(s) 538
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 2 cut(s) 540, 615
BmrFI CCNGG 3 cut(s) 246, 327, 738
BmsI GCATC 3 cut(s) 205, 541, 713
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 771, 846
BsaJI CCNNGG 2 cut(s) 150, 245
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 721
Bse1I ACTGG 1 cut(s) 496
BseBI CCWGG 2 cut(s) 246, 327
BseDI CCNNGG 2 cut(s) 150, 245
BseGI GGATG 2 cut(s) 820, 864
BseLI CCNNNNNNNGG 2 cut(s) 151, 721
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 3 cut(s) 127, 704, 799
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 5 cut(s) 4, 50, 154, 661, 833
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 514, 729
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 2 cut(s) 151, 721
BsmAI GTCTC 3 cut(s) 192, 771, 846
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 2 cut(s) 771, 846
BsoBI CYCGRG 1 cut(s) 45
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 514, 729
Bsp143I GATC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 4 cut(s) 81, 120, 123, 145
BspANI GGCC 1 cut(s) 155
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 2 cut(s) 540, 615
BspT104I TTCGAA 1 cut(s) 473
BspTNI GGTCTC 2 cut(s) 771, 846
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 150, 245
BssMI GATC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 246, 327
Bst4CI ACNGT 2 cut(s) 13, 407
Bst6I CTCTTC 1 cut(s) 776
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 3 cut(s) 591, 834, 876
BstDEI CTNAG 1 cut(s) 609
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 820, 864
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 2 cut(s) 243, 426
BstHHI GCGC 3 cut(s) 149, 242, 425
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 3 cut(s) 192, 771, 846
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 4 cut(s) 47, 237, 560, 599
BstNI CCWGG 2 cut(s) 246, 327
BstNSI RCATGY 1 cut(s) 828
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 244, 325, 736
BstSLI GKGCMC 1 cut(s) 729
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 4, 50, 154, 661, 833
BstXI CCANNNNNNTGG 1 cut(s) 685
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 2 cut(s) 820, 864
Cac8I GCNNGC 3 cut(s) 591, 834, 876
CaiI CAGNNNCTG 2 cut(s) 599, 776
CciI TCATGA 1 cut(s) 756
CfoI GCGC 3 cut(s) 149, 242, 425
Cfr13I GGNCC 1 cut(s) 538
CseI GACGC 1 cut(s) 118
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 8 cut(s) 151, 299, 502, 749, 757, 796, 825, 855
DdeI CTNAG 1 cut(s) 609
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 771, 846
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 244, 325
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 8 cut(s) 154, 302, 505, 752, 760, 799, 828, 858
FaqI GGGAC 1 cut(s) 524
FatI CATG 8 cut(s) 150, 298, 501, 748, 756, 795, 824, 854
FauI CCCGC 1 cut(s) 127
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 18, 39, 123, 168, 675, 822
FokI GGATG 2 cut(s) 827, 871
Fsp4HI GCNGC 6 cut(s) 18, 39, 123, 168, 675, 822
GlaI GCGC 3 cut(s) 148, 241, 424
GluI GCNGC 6 cut(s) 18, 39, 123, 168, 675, 822
GsuI CTGGAG 1 cut(s) 753
HaeII RGCGCY 2 cut(s) 243, 426
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 738
HgaI GACGC 1 cut(s) 118
HhaI GCGC 3 cut(s) 149, 242, 425
Hin1II CATG 8 cut(s) 154, 302, 505, 752, 760, 799, 828, 858
Hin6I GCGC 3 cut(s) 147, 240, 423
HinP1I GCGC 3 cut(s) 147, 240, 423
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 3 cut(s) 161, 330, 711
HpaII CCGG 1 cut(s) 738
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 3 cut(s) 160, 586, 664
Hpy188III TCNNGA 7 cut(s) 45, 263, 307, 410, 708, 757, 770
Hpy8I GTNNAC 1 cut(s) 727
HpyAV CCTTC 7 cut(s) 61, 103, 151, 184, 358, 472, 784
HpyCH4III ACNGT 2 cut(s) 13, 407
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 7 cut(s) 218, 383, 501, 524, 563, 593, 727
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 237, 560, 599
HpyF3I CTNAG 1 cut(s) 609
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 8 cut(s) 154, 302, 505, 752, 760, 799, 828, 858
HspAI GCGC 3 cut(s) 147, 240, 423
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 4 cut(s) 140, 247, 328, 619
Lsp1109I GCAGC 5 cut(s) 4, 50, 154, 661, 833
LweI GCATC 3 cut(s) 205, 541, 713
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 2 cut(s) 487, 667
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 4 cut(s) 46, 49, 487, 793
MfeI CAATTG 1 cut(s) 519
MhlI GDGCHC 2 cut(s) 514, 729
MluCI AATT 3 cut(s) 288, 451, 519
MnlI CCTC 7 cut(s) 148, 166, 682, 715, 777, 780, 805
Mph1103I ATGCAT 1 cut(s) 220
MseI TTAA 3 cut(s) 291, 450, 880
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 1 cut(s) 738
MspR9I CCNGG 3 cut(s) 246, 327, 738
MunI CAATTG 1 cut(s) 519
MvaI CCWGG 2 cut(s) 246, 327
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 47, 237, 560, 599
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 1 cut(s) 150
NdeI CATATG 1 cut(s) 220
NdeII GATC 1 cut(s) 32
NlaIII CATG 8 cut(s) 154, 302, 505, 752, 760, 799, 828, 858
NlaIV GGNNCC 2 cut(s) 540, 615
NmuCI GTSAC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 828
NspV TTCGAA 1 cut(s) 473
PagI TCATGA 1 cut(s) 756
PfeI GAWTC 3 cut(s) 161, 330, 711
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 6 cut(s) 19, 40, 124, 169, 676, 823
PshAI GACNNNNGTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 244, 325
PspGI CCWGG 2 cut(s) 244, 325
PspN4I GGNNCC 2 cut(s) 540, 615
PspPI GGNCC 1 cut(s) 538
PstNI CAGNNNCTG 2 cut(s) 599, 776
PvuII CAGCTG 1 cut(s) 832
SaqAI TTAA 3 cut(s) 291, 450, 880
SatI GCNGC 6 cut(s) 18, 39, 123, 168, 675, 822
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 1 cut(s) 538
ScrFI CCNGG 3 cut(s) 246, 327, 738
SduI GDGCHC 2 cut(s) 514, 729
SfaNI GCATC 3 cut(s) 205, 541, 713
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 1 cut(s) 538
Sse9I AATT 3 cut(s) 288, 451, 519
SsiI CCGC 4 cut(s) 81, 120, 123, 145
StyD4I CCNGG 3 cut(s) 244, 325, 736
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 13, 407
TaiI ACGT 1 cut(s) 669
TaqI TCGA 1 cut(s) 473
TasI AATT 3 cut(s) 288, 451, 519
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 3 cut(s) 161, 330, 711
Tru1I TTAA 3 cut(s) 291, 450, 880
Tru9I TTAA 3 cut(s) 291, 450, 880
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 5 cut(s) 17, 38, 167, 674, 821
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 300, 745, 773, 831
TspGWI ACGGA 1 cut(s) 121
VneI GTGCAC 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 538
XceI RCATGY 1 cut(s) 828
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.