Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1246 |
| AccBSI | CCGCTC | 1 cut(s) | 502 |
| AciI | CCGC | 1 cut(s) | 502 |
| AclWI | GGATC | 4 cut(s) | 540, 553, 1103, 1563 |
| AcsI | RAATTY | 4 cut(s) | 46, 140, 154, 189 |
| AdeI | CACNNNGTG | 1 cut(s) | 455 |
| AfaI | GTAC | 3 cut(s) | 425, 581, 1004 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 542 |
| AflIII | ACRYGT | 2 cut(s) | 1005, 1566 |
| AgeI | ACCGGT | 1 cut(s) | 325 |
| AgsI | TTSAA | 7 cut(s) | 153, 163, 283, 389, 577, 733, 994 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1171 |
| AhlI | ACTAGT | 1 cut(s) | 874 |
| AjiI | CACGTC | 1 cut(s) | 1567 |
| AjnI | CCWGG | 3 cut(s) | 1107, 1126, 1315 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 428, 460 |
| Alw26I | GTCTC | 1 cut(s) | 1431 |
| AlwI | GGATC | 4 cut(s) | 540, 553, 1103, 1563 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 767 |
| Ama87I | CYCGRG | 1 cut(s) | 895 |
| ApeKI | GCWGC | 7 cut(s) | 299, 415, 1265, 1268, 1281, 1351, 1475 |
| ApoI | RAATTY | 4 cut(s) | 46, 140, 154, 189 |
| AseI | ATTAAT | 1 cut(s) | 648 |
| AsiGI | ACCGGT | 1 cut(s) | 325 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 840 |
| AspS9I | GGNCC | 3 cut(s) | 614, 698, 1133 |
| AsuHPI | GGTGA | 4 cut(s) | 88, 441, 845, 1450 |
| AsuII | TTCGAA | 1 cut(s) | 193 |
| AvaI | CYCGRG | 1 cut(s) | 895 |
| AvaII | GGWCC | 3 cut(s) | 614, 698, 1133 |
| BamHI | GGATCC | 1 cut(s) | 545 |
| BanI | GGYRCC | 1 cut(s) | 1246 |
| BbvI | GCAGC | 7 cut(s) | 311, 402, 1252, 1268, 1280, 1338, 1462 |
| BccI | CCATC | 2 cut(s) | 811, 1398 |
| BceAI | ACGGC | 2 cut(s) | 1234, 1256 |
| BciT130I | CCWGG | 3 cut(s) | 1109, 1128, 1317 |
| BcoDI | GTCTC | 1 cut(s) | 1431 |
| BcuI | ACTAGT | 1 cut(s) | 874 |
| BfaI | CTAG | 2 cut(s) | 453, 875 |
| BfmI | CTRYAG | 2 cut(s) | 526, 1266 |
| BisI | GCNGC | 7 cut(s) | 300, 416, 1266, 1269, 1282, 1352, 1476 |
| BlsI | GCNGC | 7 cut(s) | 301, 417, 1267, 1270, 1283, 1353, 1477 |
| Bme1390I | CCNGG | 3 cut(s) | 1109, 1128, 1317 |
| Bme18I | GGWCC | 3 cut(s) | 614, 698, 1133 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1171 |
| BmeT110I | CYCGRG | 1 cut(s) | 895 |
| BmgBI | CACGTC | 1 cut(s) | 1567 |
| BmgT120I | GGNCC | 3 cut(s) | 614, 698, 1133 |
| BmiI | GGNNCC | 7 cut(s) | 200, 378, 547, 615, 699, 1021, 1248 |
| BmrFI | CCNGG | 3 cut(s) | 1109, 1128, 1317 |
| BmsI | GCATC | 6 cut(s) | 300, 553, 899, 914, 1127, 1309 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1411, 1443 |
| BpmI | CTGGAG | 1 cut(s) | 1316 |
| Bpu14I | TTCGAA | 1 cut(s) | 193 |
| BsaBI | GATNNNNATC | 1 cut(s) | 774 |
| BsaJI | CCNNGG | 5 cut(s) | 372, 894, 1186, 1316, 1550 |
| BsaWI | WCCGGW | 1 cut(s) | 325 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 542 |
| Bse118I | RCCGGY | 1 cut(s) | 325 |
| Bse1I | ACTGG | 2 cut(s) | 58, 131 |
| Bse3DI | GCAATG | 1 cut(s) | 1023 |
| Bse8I | GATNNNNATC | 1 cut(s) | 774 |
| BseBI | CCWGG | 3 cut(s) | 1109, 1128, 1317 |
| BseDI | CCNNGG | 5 cut(s) | 372, 894, 1186, 1316, 1550 |
| BseGI | GGATG | 5 cut(s) | 322, 801, 808, 890, 1118 |
| BseJI | GATNNNNATC | 1 cut(s) | 774 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BseMI | GCAATG | 1 cut(s) | 1023 |
| BseMII | CTCAG | 4 cut(s) | 489, 911, 1224, 1397 |
| BseNI | ACTGG | 2 cut(s) | 58, 131 |
| BseRI | GAGGAG | 2 cut(s) | 941, 1206 |
| BseXI | GCAGC | 7 cut(s) | 311, 402, 1252, 1268, 1280, 1338, 1462 |
| BseYI | CCCAGC | 3 cut(s) | 394, 610, 693 |
| BsgI | GTGCAG | 1 cut(s) | 1337 |
| BshNI | GGYRCC | 1 cut(s) | 1246 |
| BshTI | ACCGGT | 1 cut(s) | 325 |
| BsiHKCI | CYCGRG | 1 cut(s) | 895 |
| BsiSI | CCGG | 1 cut(s) | 326 |
| BslFI | GGGAC | 2 cut(s) | 711, 969 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BsmAI | GTCTC | 1 cut(s) | 1431 |
| BsmFI | GGGAC | 2 cut(s) | 711, 969 |
| BsmI | GAATGC | 1 cut(s) | 1343 |
| BsoBI | CYCGRG | 1 cut(s) | 895 |
| Bsp119I | TTCGAA | 1 cut(s) | 193 |
| Bsp143I | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| BspACI | CCGC | 1 cut(s) | 502 |
| BspCNI | CTCAG | 4 cut(s) | 490, 912, 1225, 1396 |
| BspLI | GGNNCC | 7 cut(s) | 200, 378, 547, 615, 699, 1021, 1248 |
| BspMAI | CTGCAG | 1 cut(s) | 1270 |
| BspPI | GGATC | 4 cut(s) | 540, 553, 1103, 1563 |
| BspT104I | TTCGAA | 1 cut(s) | 193 |
| BspT107I | GGYRCC | 1 cut(s) | 1246 |
| BsrBI | CCGCTC | 1 cut(s) | 502 |
| BsrDI | GCAATG | 1 cut(s) | 1023 |
| BsrFI | RCCGGY | 1 cut(s) | 325 |
| BsrI | ACTGG | 2 cut(s) | 58, 131 |
| BssAI | RCCGGY | 1 cut(s) | 325 |
| BssECI | CCNNGG | 5 cut(s) | 372, 894, 1186, 1316, 1550 |
| BssMI | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| Bst2UI | CCWGG | 3 cut(s) | 1109, 1128, 1317 |
| Bst4CI | ACNGT | 2 cut(s) | 114, 604 |
| Bst6I | CTCTTC | 3 cut(s) | 78, 284, 1551 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1360 |
| BstBI | TTCGAA | 1 cut(s) | 193 |
| BstC8I | GCNNGC | 4 cut(s) | 185, 289, 369, 667 |
| BstDEI | CTNAG | 4 cut(s) | 498, 920, 1233, 1383 |
| BstDSI | CCRYGG | 1 cut(s) | 1186 |
| BstEII | GGTNACC | 1 cut(s) | 1438 |
| BstF5I | GGATG | 5 cut(s) | 322, 801, 808, 890, 1118 |
| BstKTI | GATC | 4 cut(s) | 548, 778, 1098, 1558 |
| BstMAI | GTCTC | 1 cut(s) | 1431 |
| BstMBI | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 338, 887, 1019, 1360 |
| BstNI | CCWGG | 3 cut(s) | 1109, 1128, 1317 |
| BstNSI | RCATGY | 3 cut(s) | 964, 1009, 1363 |
| BstPI | GGTNACC | 1 cut(s) | 1438 |
| BstSCI | CCNGG | 3 cut(s) | 1107, 1126, 1315 |
| BstSFI | CTRYAG | 2 cut(s) | 526, 1266 |
| BstV1I | GCAGC | 7 cut(s) | 311, 402, 1252, 1268, 1280, 1338, 1462 |
| BstX2I | RGATCY | 1 cut(s) | 545 |
| BstYI | RGATCY | 1 cut(s) | 545 |
| BtgI | CCRYGG | 1 cut(s) | 1186 |
| BtrI | CACGTC | 1 cut(s) | 1567 |
| BtsCI | GGATG | 5 cut(s) | 322, 801, 808, 890, 1118 |
| BtsI | GCAGTG | 1 cut(s) | 21 |
| BtsIMutI | CAGTG | 5 cut(s) | 21, 51, 110, 917, 1097 |
| Cac8I | GCNNGC | 4 cut(s) | 185, 289, 369, 667 |
| CaiI | CAGNNNCTG | 1 cut(s) | 767 |
| Cfr10I | RCCGGY | 1 cut(s) | 325 |
| Cfr13I | GGNCC | 3 cut(s) | 614, 698, 1133 |
| CsiI | ACCWGGT | 1 cut(s) | 1126 |
| Csp6I | GTAC | 3 cut(s) | 424, 580, 1003 |
| CspAI | ACCGGT | 1 cut(s) | 325 |
| CviAII | CATG | 8 cut(s) | 685, 961, 1006, 1036, 1171, 1262, 1348, 1360 |
| CviQI | GTAC | 3 cut(s) | 424, 580, 1003 |
| DdeI | CTNAG | 4 cut(s) | 498, 920, 1233, 1383 |
| DpnI | GATC | 4 cut(s) | 547, 777, 1097, 1557 |
| DpnII | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| DraIII | CACNNNGTG | 1 cut(s) | 455 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1171 |
| Eam1104I | CTCTTC | 3 cut(s) | 78, 284, 1551 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1171 |
| EarI | CTCTTC | 3 cut(s) | 78, 284, 1551 |
| Eco47I | GGWCC | 3 cut(s) | 614, 698, 1133 |
| Eco88I | CYCGRG | 1 cut(s) | 895 |
| Eco91I | GGTNACC | 1 cut(s) | 1438 |
| EcoO109I | RGGNCCY | 1 cut(s) | 614 |
| EcoO65I | GGTNACC | 1 cut(s) | 1438 |
| EcoRI | GAATTC | 2 cut(s) | 140, 189 |
| EcoRII | CCWGG | 3 cut(s) | 1107, 1126, 1315 |
| FaeI | CATG | 8 cut(s) | 688, 964, 1009, 1039, 1174, 1265, 1351, 1363 |
| FaqI | GGGAC | 2 cut(s) | 711, 969 |
| FatI | CATG | 8 cut(s) | 684, 960, 1005, 1035, 1170, 1261, 1347, 1359 |
| Fnu4HI | GCNGC | 7 cut(s) | 300, 416, 1266, 1269, 1282, 1352, 1476 |
| FokI | GGATG | 5 cut(s) | 329, 808, 815, 877, 1105 |
| Fsp4HI | GCNGC | 7 cut(s) | 300, 416, 1266, 1269, 1282, 1352, 1476 |
| FspBI | CTAG | 2 cut(s) | 453, 875 |
| GluI | GCNGC | 7 cut(s) | 300, 416, 1266, 1269, 1282, 1352, 1476 |
| GsaI | CCCAGC | 3 cut(s) | 398, 614, 697 |
| GsuI | CTGGAG | 1 cut(s) | 1316 |
| HapII | CCGG | 1 cut(s) | 326 |
| Hin1II | CATG | 8 cut(s) | 688, 964, 1009, 1039, 1174, 1265, 1351, 1363 |
| HincII | GTYRAC | 1 cut(s) | 264 |
| HindII | GTYRAC | 1 cut(s) | 264 |
| HindIII | AAGCTT | 1 cut(s) | 147 |
| HpaII | CCGG | 1 cut(s) | 326 |
| HphI | GGTGA | 4 cut(s) | 88, 441, 845, 1450 |
| Hpy166II | GTNNAC | 4 cut(s) | 264, 836, 1057, 1133 |
| Hpy188III | TCNNGA | 4 cut(s) | 617, 632, 1122, 1394 |
| Hpy8I | GTNNAC | 4 cut(s) | 264, 836, 1057, 1133 |
| Hpy99I | CGWCG | 1 cut(s) | 1289 |
| HpyAV | CCTTC | 6 cut(s) | 189, 233, 793, 863, 880, 1033 |
| HpyCH4III | ACNGT | 2 cut(s) | 114, 604 |
| HpyCH4IV | ACGT | 2 cut(s) | 1287, 1566 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 338, 887, 1019, 1360 |
| HpyF3I | CTNAG | 4 cut(s) | 498, 920, 1233, 1383 |
| HpySE526I | ACGT | 2 cut(s) | 1287, 1566 |
| Hsp92II | CATG | 8 cut(s) | 688, 964, 1009, 1039, 1174, 1265, 1351, 1363 |
| Kzo9I | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| LmnI | GCTCC | 4 cut(s) | 204, 662, 1019, 1297 |
| Lsp1109I | GCAGC | 7 cut(s) | 311, 402, 1252, 1268, 1280, 1338, 1462 |
| LweI | GCATC | 6 cut(s) | 300, 553, 899, 914, 1127, 1309 |
| MabI | ACCWGGT | 1 cut(s) | 1126 |
| MaeI | CTAG | 2 cut(s) | 453, 875 |
| MaeII | ACGT | 2 cut(s) | 1287, 1566 |
| MaeIII | GTNAC | 6 cut(s) | 102, 468, 807, 902, 1438, 1540 |
| MalI | GATC | 4 cut(s) | 547, 777, 1097, 1557 |
| MbiI | CCGCTC | 1 cut(s) | 502 |
| MboI | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| MboII | GAAGA | 9 cut(s) | 65, 68, 71, 74, 77, 271, 739, 742, 1538 |
| MflI | RGATCY | 1 cut(s) | 545 |
| MluCI | AATT | 8 cut(s) | 46, 140, 154, 189, 593, 649, 1074, 1507 |
| MlyI | GAGTC | 6 cut(s) | 110, 269, 473, 1096, 1160, 1538 |
| MroXI | GAANNNNTTC | 1 cut(s) | 840 |
| MseI | TTAA | 4 cut(s) | 648, 782, 1242, 1416 |
| MslI | CAYNNNNRTG | 3 cut(s) | 922, 1305, 1520 |
| MspA1I | CMGCKG | 2 cut(s) | 693, 1294 |
| MspI | CCGG | 1 cut(s) | 326 |
| MspR9I | CCNGG | 3 cut(s) | 1109, 1128, 1317 |
| Mva1269I | GAATGC | 1 cut(s) | 1343 |
| MvaI | CCWGG | 3 cut(s) | 1109, 1128, 1317 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 338, 887, 1019, 1360 |
| NdeII | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| NlaIII | CATG | 8 cut(s) | 688, 964, 1009, 1039, 1174, 1265, 1351, 1363 |
| NlaIV | GGNNCC | 7 cut(s) | 200, 378, 547, 615, 699, 1021, 1248 |
| NmuCI | GTSAC | 3 cut(s) | 102, 468, 1438 |
| NspI | RCATGY | 3 cut(s) | 964, 1009, 1363 |
| NspV | TTCGAA | 1 cut(s) | 193 |
| PciI | ACATGT | 1 cut(s) | 1005 |
| PctI | GAATGC | 1 cut(s) | 1343 |
| PdmI | GAANNNNTTC | 1 cut(s) | 840 |
| PfeI | GAWTC | 4 cut(s) | 620, 710, 841, 1457 |
| PinAI | ACCGGT | 1 cut(s) | 325 |
| PkrI | GCNGC | 7 cut(s) | 301, 417, 1267, 1270, 1283, 1353, 1477 |
| PleI | GAGTC | 6 cut(s) | 109, 268, 473, 1095, 1160, 1537 |
| PpsI | GAGTC | 6 cut(s) | 109, 268, 473, 1095, 1160, 1537 |
| PpuMI | RGGWCCY | 1 cut(s) | 614 |
| PscI | ACATGT | 1 cut(s) | 1005 |
| PshBI | ATTAAT | 1 cut(s) | 648 |
| Psp5II | RGGWCCY | 1 cut(s) | 614 |
| Psp6I | CCWGG | 3 cut(s) | 1107, 1126, 1315 |
| PspEI | GGTNACC | 1 cut(s) | 1438 |
| PspFI | CCCAGC | 3 cut(s) | 394, 610, 693 |
| PspGI | CCWGG | 3 cut(s) | 1107, 1126, 1315 |
| PspN4I | GGNNCC | 7 cut(s) | 200, 378, 547, 615, 699, 1021, 1248 |
| PspPI | GGNCC | 3 cut(s) | 614, 698, 1133 |
| PspPPI | RGGWCCY | 1 cut(s) | 614 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 52, 84 |
| PstI | CTGCAG | 1 cut(s) | 1270 |
| PstNI | CAGNNNCTG | 1 cut(s) | 767 |
| PsuI | RGATCY | 1 cut(s) | 545 |
| PvuII | CAGCTG | 2 cut(s) | 693, 1294 |
| RsaI | GTAC | 3 cut(s) | 425, 581, 1004 |
| RsaNI | GTAC | 3 cut(s) | 424, 580, 1003 |
| RseI | CAYNNNNRTG | 3 cut(s) | 922, 1305, 1520 |
| SaqAI | TTAA | 4 cut(s) | 648, 782, 1242, 1416 |
| SatI | GCNGC | 7 cut(s) | 300, 416, 1266, 1269, 1282, 1352, 1476 |
| Sau3AI | GATC | 4 cut(s) | 545, 775, 1095, 1555 |
| Sau96I | GGNCC | 3 cut(s) | 614, 698, 1133 |
| SchI | GAGTC | 6 cut(s) | 110, 269, 473, 1096, 1160, 1538 |
| ScrFI | CCNGG | 3 cut(s) | 1109, 1128, 1317 |
| SexAI | ACCWGGT | 1 cut(s) | 1126 |
| SfaNI | GCATC | 6 cut(s) | 300, 553, 899, 914, 1127, 1309 |
| SfcI | CTRYAG | 2 cut(s) | 526, 1266 |
| SfuI | TTCGAA | 1 cut(s) | 193 |
| SinI | GGWCC | 3 cut(s) | 614, 698, 1133 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 922, 1305, 1520 |
| SpeI | ACTAGT | 1 cut(s) | 874 |
| Sse9I | AATT | 8 cut(s) | 46, 140, 154, 189, 593, 649, 1074, 1507 |
| SsiI | CCGC | 1 cut(s) | 502 |
| SspI | AATATT | 2 cut(s) | 628, 1204 |
| SspMI | CTAG | 2 cut(s) | 453, 875 |
| StyD4I | CCNGG | 3 cut(s) | 1107, 1126, 1315 |
| TaaI | ACNGT | 2 cut(s) | 114, 604 |
| TaiI | ACGT | 2 cut(s) | 1290, 1569 |
| TaqI | TCGA | 4 cut(s) | 193, 294, 1312, 1532 |
| TaqII | GACCGA | 1 cut(s) | 715 |
| TasI | AATT | 8 cut(s) | 46, 140, 154, 189, 593, 649, 1074, 1507 |
| TatI | WGTACW | 1 cut(s) | 579 |
| TfiI | GAWTC | 4 cut(s) | 620, 710, 841, 1457 |
| Tru1I | TTAA | 4 cut(s) | 648, 782, 1242, 1416 |
| Tru9I | TTAA | 4 cut(s) | 648, 782, 1242, 1416 |
| TscAI | CASTG | 5 cut(s) | 21, 58, 117, 924, 1097 |
| TseFI | GTSAC | 3 cut(s) | 102, 468, 1438 |
| TseI | GCWGC | 7 cut(s) | 299, 415, 1265, 1268, 1281, 1351, 1475 |
| Tsp45I | GTSAC | 3 cut(s) | 102, 468, 1438 |
| TspDTI | ATGAA | 8 cut(s) | 147, 477, 497, 812, 1052, 1250, 1336, 1349 |
| TspGWI | ACGGA | 3 cut(s) | 483, 898, 1136 |
| TspRI | CASTG | 5 cut(s) | 21, 58, 117, 924, 1097 |
| VpaK11BI | GGWCC | 3 cut(s) | 614, 698, 1133 |
| VspI | ATTAAT | 1 cut(s) | 648 |
| XapI | RAATTY | 4 cut(s) | 46, 140, 154, 189 |
| XceI | RCATGY | 3 cut(s) | 964, 1009, 1363 |
| XmnI | GAANNNNTTC | 1 cut(s) | 840 |
| XspI | CTAG | 2 cut(s) | 453, 875 |