Belongs to the GRAS family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 976 |
| AccB1I | GGYRCC | 1 cut(s) | 976 |
| AccBSI | CCGCTC | 1 cut(s) | 1297 |
| AciI | CCGC | 2 cut(s) | 1297, 1436 |
| AclI | AACGTT | 1 cut(s) | 577 |
| AclWI | GGATC | 3 cut(s) | 829, 833, 1307 |
| AcsI | RAATTY | 3 cut(s) | 535, 1002, 1066 |
| AfaI | GTAC | 4 cut(s) | 734, 978, 1378, 1496 |
| AfeI | AGCGCT | 1 cut(s) | 1249 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 627, 1566 |
| AflIII | ACRYGT | 2 cut(s) | 591, 735 |
| AgsI | TTSAA | 7 cut(s) | 59, 179, 463, 724, 1043, 1263, 1505 |
| AjnI | CCWGG | 2 cut(s) | 837, 856 |
| Alw26I | GTCTC | 4 cut(s) | 10, 267, 1161, 1223 |
| AlwI | GGATC | 3 cut(s) | 829, 833, 1307 |
| Aor51HI | AGCGCT | 1 cut(s) | 1249 |
| AoxI | GGCC | 4 cut(s) | 301, 618, 854, 863 |
| ApeKI | GCWGC | 5 cut(s) | 49, 816, 995, 1081, 1312 |
| ApoI | RAATTY | 3 cut(s) | 535, 1002, 1066 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 28 |
| Asp718I | GGTACC | 1 cut(s) | 976 |
| AspA2I | CCTAGG | 1 cut(s) | 1121 |
| AspLEI | GCGC | 1 cut(s) | 1250 |
| AspS9I | GGNCC | 3 cut(s) | 863, 1030, 1126 |
| AsuHPI | GGTGA | 5 cut(s) | 56, 467, 820, 1061, 1419 |
| AvaII | GGWCC | 2 cut(s) | 1030, 1126 |
| AvrII | CCTAGG | 1 cut(s) | 1121 |
| BanI | GGYRCC | 1 cut(s) | 976 |
| BauI | CACGAG | 1 cut(s) | 1326 |
| BbvCI | CCTCAGC | 1 cut(s) | 154 |
| BbvI | GCAGC | 5 cut(s) | 61, 828, 982, 1068, 1324 |
| BccI | CCATC | 4 cut(s) | 196, 430, 541, 1508 |
| BciT130I | CCWGG | 2 cut(s) | 839, 858 |
| BcoDI | GTCTC | 4 cut(s) | 10, 267, 1161, 1223 |
| BfaI | CTAG | 5 cut(s) | 192, 255, 279, 669, 1122 |
| BfmI | CTRYAG | 2 cut(s) | 624, 1475 |
| BfoI | RGCGCY | 1 cut(s) | 1251 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 749, 862 |
| BisI | GCNGC | 5 cut(s) | 50, 817, 996, 1082, 1313 |
| BlnI | CCTAGG | 1 cut(s) | 1121 |
| BlpI | GCTNAGC | 1 cut(s) | 1439 |
| BlsI | GCNGC | 5 cut(s) | 51, 818, 997, 1083, 1314 |
| Bme1390I | CCNGG | 2 cut(s) | 839, 858 |
| Bme18I | GGWCC | 2 cut(s) | 1030, 1126 |
| BmgT120I | GGNCC | 3 cut(s) | 863, 1030, 1126 |
| BmiI | GGNNCC | 3 cut(s) | 978, 1127, 1519 |
| BmrFI | CCNGG | 2 cut(s) | 839, 858 |
| BmsI | GCATC | 7 cut(s) | 78, 384, 644, 645, 653, 911, 1119 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 137, 169, 431, 463, 1141, 1173 |
| BpmI | CTGGAG | 2 cut(s) | 433, 879 |
| Bpu10I | CCTNAGC | 2 cut(s) | 154, 305 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1439 |
| BpuEI | CTTGAG | 2 cut(s) | 795, 1046 |
| BsaI | GGTCTC | 1 cut(s) | 267 |
| BsaJI | CCNNGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 627, 1566 |
| Bse118I | RCCGGY | 1 cut(s) | 1128 |
| Bse1I | ACTGG | 6 cut(s) | 53, 162, 250, 274, 428, 820 |
| BseBI | CCWGG | 2 cut(s) | 839, 858 |
| BseDI | CCNNGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| BseGI | GGATG | 5 cut(s) | 102, 149, 778, 1110, 1542 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 627, 1566 |
| BseMII | CTCAG | 7 cut(s) | 168, 219, 296, 1005, 1218, 1402, 1479 |
| BseNI | ACTGG | 6 cut(s) | 53, 162, 250, 274, 428, 820 |
| BseXI | GCAGC | 5 cut(s) | 61, 828, 982, 1068, 1324 |
| BsgI | GTGCAG | 1 cut(s) | 1067 |
| BshFI | GGCC | 4 cut(s) | 303, 620, 856, 865 |
| BshNI | GGYRCC | 1 cut(s) | 976 |
| BsiSI | CCGG | 1 cut(s) | 1129 |
| BslFI | GGGAC | 3 cut(s) | 939, 1139, 1200 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 627, 1566 |
| BsmAI | GTCTC | 4 cut(s) | 10, 267, 1161, 1223 |
| BsmFI | GGGAC | 3 cut(s) | 939, 1139, 1200 |
| BsnI | GGCC | 4 cut(s) | 303, 620, 856, 865 |
| Bso31I | GGTCTC | 1 cut(s) | 267 |
| Bsp143I | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1439 |
| BspACI | CCGC | 2 cut(s) | 1297, 1436 |
| BspANI | GGCC | 4 cut(s) | 303, 620, 856, 865 |
| BspCNI | CTCAG | 7 cut(s) | 167, 218, 297, 1006, 1219, 1401, 1480 |
| BspLI | GGNNCC | 3 cut(s) | 978, 1127, 1519 |
| BspMAI | CTGCAG | 1 cut(s) | 1479 |
| BspPI | GGATC | 3 cut(s) | 829, 833, 1307 |
| BspQI | GCTCTTC | 2 cut(s) | 54, 408 |
| BspT107I | GGYRCC | 1 cut(s) | 976 |
| BspTNI | GGTCTC | 1 cut(s) | 267 |
| BsrBI | CCGCTC | 1 cut(s) | 1297 |
| BsrFI | RCCGGY | 1 cut(s) | 1128 |
| BsrI | ACTGG | 6 cut(s) | 53, 162, 250, 274, 428, 820 |
| BssAI | RCCGGY | 1 cut(s) | 1128 |
| BssECI | CCNNGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| BssMI | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| BssSI | CACGAG | 1 cut(s) | 1326 |
| BssT1I | CCWWGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| Bst2BI | CACGAG | 1 cut(s) | 1326 |
| Bst2UI | CCWGG | 2 cut(s) | 839, 858 |
| Bst4CI | ACNGT | 2 cut(s) | 296, 1319 |
| Bst6I | CTCTTC | 2 cut(s) | 54, 408 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1090 |
| BstC8I | GCNNGC | 4 cut(s) | 6, 566, 666, 960 |
| BstDEI | CTNAG | 9 cut(s) | 133, 154, 205, 305, 1014, 1227, 1388, 1439, 1488 |
| BstF5I | GGATG | 5 cut(s) | 102, 149, 778, 1110, 1542 |
| BstH2I | RGCGCY | 1 cut(s) | 1251 |
| BstHHI | GCGC | 1 cut(s) | 1250 |
| BstKTI | GATC | 5 cut(s) | 139, 447, 828, 837, 1302 |
| BstMAI | GTCTC | 4 cut(s) | 10, 267, 1161, 1223 |
| BstMBI | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 238, 617, 749, 862, 1090, 1245 |
| BstNI | CCWGG | 2 cut(s) | 839, 858 |
| BstNSI | RCATGY | 4 cut(s) | 595, 694, 739, 1093 |
| BstSCI | CCNGG | 2 cut(s) | 837, 856 |
| BstSFI | CTRYAG | 2 cut(s) | 624, 1475 |
| BstV1I | GCAGC | 5 cut(s) | 61, 828, 982, 1068, 1324 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 257, 998 |
| BsuRI | GGCC | 4 cut(s) | 303, 620, 856, 865 |
| BtsCI | GGATG | 5 cut(s) | 102, 149, 778, 1110, 1542 |
| BtsI | GCAGTG | 1 cut(s) | 1068 |
| BtsIMutI | CAGTG | 3 cut(s) | 827, 1068, 1315 |
| Cac8I | GCNNGC | 4 cut(s) | 6, 566, 666, 960 |
| CfoI | GCGC | 1 cut(s) | 1250 |
| Cfr10I | RCCGGY | 1 cut(s) | 1128 |
| Cfr13I | GGNCC | 3 cut(s) | 863, 1030, 1126 |
| Csp6I | GTAC | 4 cut(s) | 733, 977, 1377, 1495 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1251, 1286 |
| CviQI | GTAC | 4 cut(s) | 733, 977, 1377, 1495 |
| DdeI | CTNAG | 9 cut(s) | 133, 154, 205, 305, 1014, 1227, 1388, 1439, 1488 |
| DpnI | GATC | 5 cut(s) | 138, 446, 827, 836, 1301 |
| DpnII | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| Eam1104I | CTCTTC | 2 cut(s) | 54, 408 |
| EarI | CTCTTC | 2 cut(s) | 54, 408 |
| Eco130I | CCWWGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| Eco31I | GGTCTC | 1 cut(s) | 267 |
| Eco47I | GGWCC | 2 cut(s) | 1030, 1126 |
| Eco47III | AGCGCT | 1 cut(s) | 1249 |
| EcoO109I | RGGNCCY | 2 cut(s) | 863, 1030 |
| EcoRII | CCWGG | 2 cut(s) | 837, 856 |
| EcoT14I | CCWWGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| EcoT22I | ATGCAT | 1 cut(s) | 646 |
| ErhI | CCWWGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 456, 488, 965, 997 |
| FaqI | GGGAC | 3 cut(s) | 939, 1139, 1200 |
| Fnu4HI | GCNGC | 5 cut(s) | 50, 817, 996, 1082, 1313 |
| FokI | GGATG | 5 cut(s) | 89, 136, 785, 1097, 1549 |
| Fsp4HI | GCNGC | 5 cut(s) | 50, 817, 996, 1082, 1313 |
| FspBI | CTAG | 5 cut(s) | 192, 255, 279, 669, 1122 |
| GlaI | GCGC | 1 cut(s) | 1249 |
| GluI | GCNGC | 5 cut(s) | 50, 817, 996, 1082, 1313 |
| GsuI | CTGGAG | 2 cut(s) | 433, 879 |
| HaeII | RGCGCY | 1 cut(s) | 1251 |
| HaeIII | GGCC | 4 cut(s) | 303, 620, 856, 865 |
| HapII | CCGG | 1 cut(s) | 1129 |
| HhaI | GCGC | 1 cut(s) | 1250 |
| Hin6I | GCGC | 1 cut(s) | 1248 |
| HinP1I | GCGC | 1 cut(s) | 1248 |
| HincII | GTYRAC | 2 cut(s) | 513, 1339 |
| HindII | GTYRAC | 2 cut(s) | 513, 1339 |
| HinfI | GANTC | 9 cut(s) | 68, 146, 350, 896, 965, 1171, 1187, 1259, 1540 |
| HpaI | GTTAAC | 1 cut(s) | 1339 |
| HpaII | CCGG | 1 cut(s) | 1129 |
| HphI | GGTGA | 5 cut(s) | 56, 467, 820, 1061, 1419 |
| Hpy166II | GTNNAC | 3 cut(s) | 513, 1339, 1497 |
| Hpy188I | TCNGA | 7 cut(s) | 222, 355, 412, 546, 804, 1015, 1489 |
| Hpy188III | TCNNGA | 3 cut(s) | 450, 1404, 1465 |
| Hpy8I | GTNNAC | 3 cut(s) | 513, 1339, 1497 |
| Hpy99I | CGWCG | 1 cut(s) | 296 |
| HpyAV | CCTTC | 4 cut(s) | 593, 763, 1084, 1349 |
| HpyCH4III | ACNGT | 2 cut(s) | 296, 1319 |
| HpyCH4IV | ACGT | 3 cut(s) | 541, 577, 1375 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 238, 617, 749, 862, 1090, 1245 |
| HpyF3I | CTNAG | 9 cut(s) | 133, 154, 205, 305, 1014, 1227, 1388, 1439, 1488 |
| HpySE526I | ACGT | 3 cut(s) | 541, 577, 1375 |
| HspAI | GCGC | 1 cut(s) | 1248 |
| KpnI | GGTACC | 1 cut(s) | 980 |
| KspAI | GTTAAC | 1 cut(s) | 1339 |
| Kzo9I | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| LguI | GCTCTTC | 2 cut(s) | 54, 408 |
| LmnI | GCTCC | 3 cut(s) | 584, 1448, 1517 |
| Lsp1109I | GCAGC | 5 cut(s) | 61, 828, 982, 1068, 1324 |
| LweI | GCATC | 7 cut(s) | 78, 384, 644, 645, 653, 911, 1119 |
| MaeI | CTAG | 5 cut(s) | 192, 255, 279, 669, 1122 |
| MaeII | ACGT | 3 cut(s) | 541, 577, 1375 |
| MaeIII | GTNAC | 4 cut(s) | 455, 1168, 1401, 1552 |
| MalI | GATC | 5 cut(s) | 138, 446, 827, 836, 1301 |
| MbiI | CCGCTC | 1 cut(s) | 1297 |
| MboI | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| MboII | GAAGA | 3 cut(s) | 71, 425, 1517 |
| MfeI | CAATTG | 1 cut(s) | 1263 |
| MluCI | AATT | 9 cut(s) | 166, 267, 323, 535, 792, 1002, 1066, 1263, 1332 |
| MlyI | GAGTC | 5 cut(s) | 77, 155, 359, 974, 1165 |
| MmeI | TCCRAC | 3 cut(s) | 245, 576, 598 |
| Mph1103I | ATGCAT | 1 cut(s) | 646 |
| MroXI | GAANNNNTTC | 1 cut(s) | 28 |
| MseI | TTAA | 6 cut(s) | 197, 270, 284, 650, 1146, 1338 |
| MslI | CAYNNNNRTG | 2 cut(s) | 24, 652 |
| MspI | CCGG | 1 cut(s) | 1129 |
| MspR9I | CCNGG | 2 cut(s) | 839, 858 |
| MunI | CAATTG | 1 cut(s) | 1263 |
| MvaI | CCWGG | 2 cut(s) | 839, 858 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 238, 617, 749, 862, 1090, 1245 |
| NdeII | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| NlaIV | GGNNCC | 3 cut(s) | 978, 1127, 1519 |
| NmeAIII | GCCGAG | 2 cut(s) | 832, 1509 |
| NmuCI | GTSAC | 3 cut(s) | 455, 1168, 1401 |
| NsiI | ATGCAT | 1 cut(s) | 646 |
| NspI | RCATGY | 4 cut(s) | 595, 694, 739, 1093 |
| PciI | ACATGT | 2 cut(s) | 591, 735 |
| PciSI | GCTCTTC | 2 cut(s) | 54, 408 |
| PdmI | GAANNNNTTC | 1 cut(s) | 28 |
| PfeI | GAWTC | 4 cut(s) | 896, 1187, 1259, 1540 |
| PkrI | GCNGC | 5 cut(s) | 51, 818, 997, 1083, 1314 |
| PleI | GAGTC | 5 cut(s) | 76, 154, 358, 973, 1165 |
| PpsI | GAGTC | 5 cut(s) | 76, 154, 358, 973, 1165 |
| PpuMI | RGGWCCY | 1 cut(s) | 1030 |
| PscI | ACATGT | 2 cut(s) | 591, 735 |
| Psp1406I | AACGTT | 1 cut(s) | 577 |
| Psp5II | RGGWCCY | 1 cut(s) | 1030 |
| Psp6I | CCWGG | 2 cut(s) | 837, 856 |
| PspGI | CCWGG | 2 cut(s) | 837, 856 |
| PspN4I | GGNNCC | 3 cut(s) | 978, 1127, 1519 |
| PspPI | GGNCC | 3 cut(s) | 863, 1030, 1126 |
| PspPPI | RGGWCCY | 1 cut(s) | 1030 |
| PstI | CTGCAG | 1 cut(s) | 1479 |
| RsaI | GTAC | 4 cut(s) | 734, 978, 1378, 1496 |
| RsaNI | GTAC | 4 cut(s) | 733, 977, 1377, 1495 |
| RseI | CAYNNNNRTG | 2 cut(s) | 24, 652 |
| SapI | GCTCTTC | 2 cut(s) | 54, 408 |
| SaqAI | TTAA | 6 cut(s) | 197, 270, 284, 650, 1146, 1338 |
| SatI | GCNGC | 5 cut(s) | 50, 817, 996, 1082, 1313 |
| Sau3AI | GATC | 5 cut(s) | 136, 444, 825, 834, 1299 |
| Sau96I | GGNCC | 3 cut(s) | 863, 1030, 1126 |
| SchI | GAGTC | 5 cut(s) | 77, 155, 359, 974, 1165 |
| ScrFI | CCNGG | 2 cut(s) | 839, 858 |
| SfaNI | GCATC | 7 cut(s) | 78, 384, 644, 645, 653, 911, 1119 |
| SfcI | CTRYAG | 2 cut(s) | 624, 1475 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 862 |
| SinI | GGWCC | 2 cut(s) | 1030, 1126 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 24, 652 |
| SmlI | CTYRAG | 2 cut(s) | 810, 1025 |
| SmoI | CTYRAG | 2 cut(s) | 810, 1025 |
| Sse9I | AATT | 9 cut(s) | 166, 267, 323, 535, 792, 1002, 1066, 1263, 1332 |
| SsiI | CCGC | 2 cut(s) | 1297, 1436 |
| SspMI | CTAG | 5 cut(s) | 192, 255, 279, 669, 1122 |
| StyD4I | CCNGG | 2 cut(s) | 837, 856 |
| StyI | CCWWGG | 5 cut(s) | 106, 1033, 1121, 1161, 1280 |
| TaaI | ACNGT | 2 cut(s) | 296, 1319 |
| TaiI | ACGT | 3 cut(s) | 544, 580, 1378 |
| TaqI | TCGA | 2 cut(s) | 1257, 1362 |
| TasI | AATT | 9 cut(s) | 166, 267, 323, 535, 792, 1002, 1066, 1263, 1332 |
| TatI | WGTACW | 1 cut(s) | 1494 |
| TfiI | GAWTC | 4 cut(s) | 896, 1187, 1259, 1540 |
| Tru1I | TTAA | 6 cut(s) | 197, 270, 284, 650, 1146, 1338 |
| Tru9I | TTAA | 6 cut(s) | 197, 270, 284, 650, 1146, 1338 |
| TscAI | CASTG | 3 cut(s) | 827, 1068, 1322 |
| TseFI | GTSAC | 3 cut(s) | 455, 1168, 1401 |
| TseI | GCWGC | 5 cut(s) | 49, 816, 995, 1081, 1312 |
| Tsp45I | GTSAC | 3 cut(s) | 455, 1168, 1401 |
| TspDTI | ATGAA | 6 cut(s) | 720, 776, 782, 789, 1066, 1553 |
| TspRI | CASTG | 3 cut(s) | 827, 1068, 1322 |
| VpaK11BI | GGWCC | 2 cut(s) | 1030, 1126 |
| XapI | RAATTY | 3 cut(s) | 535, 1002, 1066 |
| XceI | RCATGY | 4 cut(s) | 595, 694, 739, 1093 |
| XmaJI | CCTAGG | 1 cut(s) | 1121 |
| XmnI | GAANNNNTTC | 1 cut(s) | 28 |
| XspI | CTAG | 5 cut(s) | 192, 255, 279, 669, 1122 |
| Zsp2I | ATGCAT | 1 cut(s) | 646 |