pycom17g12400

Coproporphyrinogen-III oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011336
Physical Location & Seq
Reverse (-)
10151644 .. 10154721
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGCCACCGCCGGCGGCGCTCTCAGCTTCCTCCTCCTTCACTCTCCTCTCTCCACCTCCCTCCCCAAAACCAAAGCTCCCGCCTTTCTCCCCCAGAAAACACTCTTTCGCCATTAACCCCCGTCGCCACTCACTCCACTCCGCCCCCTCCTCCGCCGTCCGATGCGCCGTCATCGAGAAAGAGACTCCAGAGGCCCACCGCCCCGACACCTTCCTCCGCGAGAACTCCTCCTCGTCCTCCGTCCGGGGCCGCTTCGAGAAAATGATAAGAGACGCTCAGGACACCGTCTGCGCCGCCATTGAGGCGGCTGACGGCGGTGCCACGTTTAAAGAGGATGTGTGGTCGAGACCCGGCGGCGGCGGAGGCATCAGCAGGGTTCTCCAGGACGGTGCTGTTTGGGAGAAGGCTGGTGTCAATGTCTCTGTTGTGTACGGCGTTATGCCTCCCGAGGCCTACCGAGCTGCCAAGGGCGCCGCCGCAGCTGATCACAAGCCTGGCCCGGTTCCCTTCTTCGCCGCCGGAATAAGCTCGGTTTTGCATCCGAAGAACCCGTTTGCGCCTACTTTGCACTTCAATTATCGGTATTTCGAAACCGATGCTCCACAAGATGCTCCTGGAGCACCTAGGCAATGGTGGTTTGGGGGTGGAACTGACTTGACACCTGCTTATATTTTTGAGGAGGATGTTAAGCATTTTCATTCAGTTCAGAAAAGTGCTTGTGACAAATTTGATCCTGCCTTCTATCCTCGGTTCAAGAAATGGTGTGATGATTATTTCTATATCAAGCATCGAGATGAGAGGCGAGGACTTGGAGGAATATTTTTTGACGATCTAAACGATTATGATCAGGAGATGCTTCTTTCCTTTGCCACAGAATGTGCAAATTCTGTGGTCCCTGCTTACCTGCCAATTATAGAGAAAAGAAAGGACCTGCCATTTACAGATGAGCAGAAGGCATGGCAGCAATTGCGAAGAGGGCGCTACGTTGAGTTCAATTTGGTTTATGATCGCGGAACAACTTTTGGACTAAAGACAGGAGGTCGAATTGAGAGTATTCTCGTCTCTCTTCCACTGACTTCTCGATGGGAATATGACCATAAACCAGAAGAGGGAAGCGAAGAATGGAAACTATTAGATGCTTGCATCAACCCAAGGGAATGGGTTTAA

Protein Analysis

389

Amino Acids

43.44

Weight (kDa)

6.61

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coprogen_oxidas PF01218 85 - 387 9.2e-137 Coproporphyrinogen III oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03475 AT4G03205 AT4G03205
fragaria_vesca FvH4_6g39170
malus_domestica MD09G1144600.v1.1 MD17G1131200.v1.1
prunus_persica Prupe.3G183400_v2.0.a1
pyrus_communis pycom09g06400 pycom17g12400
rosa_chinensis RchiOBHm_Chr2g0153001
rosa_laevigata RLG00000020666
rosa_multiflora Rmu_sc0000218.1_g000004 Rmu_sc0000218.1_g000005
rosa_roxburghii Rroxscaffold_2G00095310
rosa_rugosa Rorug02G0436700
rosa_samantha Rh2AG498700 Rh2BG510000 Rh2CG485000 Rh2DG521600
rosa_wichuraiana Rw2G040930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 670
Acc36I ACCTGC 3 cut(s) 670, 912, 939
AccB1I GGYRCC 2 cut(s) 317, 470
AccII CGCG 2 cut(s) 219, 1011
AclWI GGATC 1 cut(s) 725
AcsI RAATTY 2 cut(s) 725, 883
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 3 cut(s) 243, 356, 1109
AgsI TTSAA 3 cut(s) 574, 754, 994
AhdI GACNNNNNGTC 1 cut(s) 1038
AjnI CCWGG 3 cut(s) 381, 493, 613
AluBI AGCT 5 cut(s) 26, 76, 461, 482, 528
AluI AGCT 5 cut(s) 26, 76, 461, 482, 528
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 5 cut(s) 176, 264, 340, 424, 1066
AlwI GGATC 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 4 cut(s) 192, 247, 450, 496
ApeKI GCWGC 3 cut(s) 461, 479, 961
ApoI RAATTY 2 cut(s) 725, 883
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 6 cut(s) 19, 167, 293, 473, 559, 981
AspS9I GGNCC 5 cut(s) 193, 247, 497, 892, 928
AsuC2I CCSGG 3 cut(s) 245, 351, 500
AsuII TTCGAA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 2 cut(s) 892, 928
AvrII CCTAGG 1 cut(s) 623
BanI GGYRCC 2 cut(s) 317, 470
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 3 cut(s) 448, 491, 973
BccI CCATC 1 cut(s) 1077
BceAI ACGGC 4 cut(s) 140, 152, 328, 448
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 3 cut(s) 383, 495, 615
BclI TGATCA 2 cut(s) 484, 844
BcnI CCSGG 3 cut(s) 245, 351, 500
BcoDI GTCTC 5 cut(s) 176, 264, 340, 424, 1066
BfaI CTAG 1 cut(s) 624
BfoI RGCGCY 3 cut(s) 20, 474, 982
BfuAI ACCTGC 3 cut(s) 670, 912, 939
BglI GCCNNNNNGGC 1 cut(s) 302
BlnI CCTAGG 1 cut(s) 623
Bme1390I CCNGG 6 cut(s) 245, 351, 383, 495, 500, 615
Bme18I GGWCC 2 cut(s) 892, 928
BmeRI GACNNNNNGTC 1 cut(s) 1038
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 5 cut(s) 193, 247, 497, 892, 928
BmiI GGNNCC 5 cut(s) 248, 319, 472, 504, 894
BmrFI CCNGG 6 cut(s) 245, 351, 383, 495, 500, 615
BmsI GCATC 9 cut(s) 152, 375, 547, 586, 598, 796, 843, 1126, 1152
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpmI CTGGAG 3 cut(s) 171, 365, 636
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 588
BpuMI CCSGG 3 cut(s) 245, 351, 500
BsaBI GATNNNNATC 1 cut(s) 843
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 340
BsaJI CCNNGG 6 cut(s) 244, 447, 465, 623, 746, 1151
BsaXI ACNNNNNCTCC 4 cut(s) 60, 90, 583, 613
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 356, 1109
Bse118I RCCGGY 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 635
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 3 cut(s) 383, 495, 615
BseDI CCNNGG 6 cut(s) 244, 447, 465, 623, 746, 1151
BseGI GGATG 3 cut(s) 340, 538, 688
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 3 cut(s) 243, 356, 1109
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 2 cut(s) 36, 290
BseRI GAGGAG 6 cut(s) 22, 35, 139, 217, 220, 692
BseXI GCAGC 3 cut(s) 448, 491, 973
Bsh1236I CGCG 2 cut(s) 219, 1011
BshFI GGCC 4 cut(s) 194, 249, 452, 498
BshNI GGYRCC 2 cut(s) 317, 470
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 5 cut(s) 11, 244, 351, 500, 519
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 3 cut(s) 243, 356, 1109
BsmAI GTCTC 5 cut(s) 176, 264, 340, 424, 1066
BsmBI CGTCTC 2 cut(s) 264, 1066
BsmFI GGGAC 1 cut(s) 878
BsnI GGCC 4 cut(s) 194, 249, 452, 498
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 446
Bsp119I TTCGAA 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 5 cut(s) 484, 730, 829, 844, 1006
BspANI GGCC 4 cut(s) 194, 249, 452, 498
BspCNI CTCAG 2 cut(s) 35, 289
BspFNI CGCG 2 cut(s) 219, 1011
BspLI GGNNCC 5 cut(s) 248, 319, 472, 504, 894
BspMI ACCTGC 3 cut(s) 670, 912, 939
BspPI GGATC 1 cut(s) 725
BspT104I TTCGAA 1 cut(s) 588
BspT107I GGYRCC 2 cut(s) 317, 470
BspTNI GGTCTC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 6 cut(s) 244, 447, 465, 623, 746, 1151
BssMI GATC 5 cut(s) 484, 730, 829, 844, 1006
BssNI GRCGYC 1 cut(s) 471
BssT1I CCWWGG 3 cut(s) 465, 623, 1151
Bst2UI CCWGG 3 cut(s) 383, 495, 615
Bst4CI ACNGT 2 cut(s) 286, 389
Bst6I CTCTTC 3 cut(s) 967, 1071, 1101
BstACI GRCGYC 1 cut(s) 471
BstAPI GCANNNNNTGC 1 cut(s) 967
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 2 cut(s) 12, 1141
BstDEI CTNAG 2 cut(s) 22, 276
BstF5I GGATG 3 cut(s) 340, 538, 688
BstFNI CGCG 2 cut(s) 219, 1011
BstH2I RGCGCY 3 cut(s) 20, 474, 982
BstHHI GCGC 6 cut(s) 19, 167, 293, 473, 559, 981
BstKTI GATC 5 cut(s) 487, 733, 832, 847, 1009
BstMAI GTCTC 5 cut(s) 176, 264, 340, 424, 1066
BstMBI GATC 5 cut(s) 484, 730, 829, 844, 1006
BstNI CCWGG 3 cut(s) 383, 495, 615
BstSCI CCNGG 6 cut(s) 243, 349, 381, 493, 498, 613
BstUI CGCG 2 cut(s) 219, 1011
BstV1I GCAGC 3 cut(s) 448, 491, 973
BstXI CCANNNNNNTGG 1 cut(s) 1158
BsuRI GGCC 4 cut(s) 194, 249, 452, 498
BtsCI GGATG 3 cut(s) 340, 538, 688
BtsIMutI CAGTG 1 cut(s) 1070
BveI ACCTGC 3 cut(s) 670, 912, 939
Cac8I GCNNGC 2 cut(s) 12, 1141
CfoI GCGC 6 cut(s) 19, 167, 293, 473, 559, 981
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 5 cut(s) 193, 247, 497, 892, 928
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 430
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 1 cut(s) 957
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 2 cut(s) 22, 276
DinI GGCGCC 1 cut(s) 472
DpnI GATC 5 cut(s) 486, 732, 831, 846, 1008
DpnII GATC 5 cut(s) 484, 730, 829, 844, 1006
DraI TTTAAA 1 cut(s) 328
DriI GACNNNNNGTC 1 cut(s) 1038
Eam1104I CTCTTC 3 cut(s) 967, 1071, 1101
Eam1105I GACNNNNNGTC 1 cut(s) 1038
EarI CTCTTC 3 cut(s) 967, 1071, 1101
EciI GGCGGA 3 cut(s) 130, 142, 375
Eco130I CCWWGG 3 cut(s) 465, 623, 1151
Eco147I AGGCCT 1 cut(s) 452
Eco31I GGTCTC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 892, 928
Eco88I CYCGRG 1 cut(s) 446
EcoO109I RGGNCCY 1 cut(s) 928
EcoRII CCWGG 3 cut(s) 381, 493, 613
EcoT14I CCWWGG 3 cut(s) 465, 623, 1151
EgeI GGCGCC 1 cut(s) 472
EheI GGCGCC 1 cut(s) 472
ErhI CCWWGG 3 cut(s) 465, 623, 1151
Esp3I CGTCTC 2 cut(s) 264, 1066
FaeI CATG 1 cut(s) 960
FaiI YATR 9 cut(s) 440, 669, 780, 843, 914, 958, 1005, 1092, 1098
FaqI GGGAC 1 cut(s) 878
FatI CATG 1 cut(s) 956
FauI CCCGC 1 cut(s) 87
FbaI TGATCA 2 cut(s) 484, 844
FokI GGATG 3 cut(s) 347, 525, 695
FspBI CTAG 1 cut(s) 624
GlaI GCGC 6 cut(s) 18, 166, 292, 472, 558, 980
GsuI CTGGAG 3 cut(s) 171, 365, 636
HaeII RGCGCY 3 cut(s) 20, 474, 982
HaeIII GGCC 4 cut(s) 194, 249, 452, 498
HapII CCGG 5 cut(s) 11, 244, 351, 500, 519
HgaI GACGC 1 cut(s) 281
HhaI GCGC 6 cut(s) 19, 167, 293, 473, 559, 981
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 1 cut(s) 960
Hin6I GCGC 6 cut(s) 17, 165, 291, 471, 557, 979
HinP1I GCGC 6 cut(s) 17, 165, 291, 471, 557, 979
HinfI GANTC 1 cut(s) 184
HpaII CCGG 5 cut(s) 11, 244, 351, 500, 519
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 3 cut(s) 161, 543, 708
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 6 cut(s) 46, 220, 397, 517, 748, 946
HpyCH4III ACNGT 2 cut(s) 286, 389
HpyCH4IV ACGT 2 cut(s) 323, 984
HpyCH4V TGCA 4 cut(s) 538, 568, 881, 1143
HpyF3I CTNAG 2 cut(s) 22, 276
HpySE526I ACGT 2 cut(s) 323, 984
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 1 cut(s) 960
HspAI GCGC 6 cut(s) 17, 165, 291, 471, 557, 979
KasI GGCGCC 1 cut(s) 470
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Ksp22I TGATCA 2 cut(s) 484, 844
Kzo9I GATC 5 cut(s) 484, 730, 829, 844, 1006
LmnI GCTCC 4 cut(s) 81, 604, 616, 617
Lsp1109I GCAGC 3 cut(s) 448, 491, 973
LweI GCATC 9 cut(s) 152, 375, 547, 586, 598, 796, 843, 1126, 1152
MaeI CTAG 1 cut(s) 624
MaeII ACGT 2 cut(s) 323, 984
MaeIII GTNAC 1 cut(s) 719
MalI GATC 5 cut(s) 486, 732, 831, 846, 1008
MboI GATC 5 cut(s) 484, 730, 829, 844, 1006
MboII GAAGA 6 cut(s) 502, 556, 984, 1058, 1118, 1130
MfeI CAATTG 1 cut(s) 965
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 7 cut(s) 574, 725, 883, 909, 965, 994, 1044
Mly113I GGCGCC 1 cut(s) 471
MlyI GAGTC 1 cut(s) 178
MreI CGCCGGCG 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 10
MseI TTAA 4 cut(s) 114, 327, 687, 1165
MslI CAYNNNNRTG 1 cut(s) 792
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 5 cut(s) 11, 244, 351, 500, 519
MspR9I CCNGG 6 cut(s) 245, 351, 383, 495, 500, 615
MunI CAATTG 1 cut(s) 965
MvaI CCWGG 3 cut(s) 383, 495, 615
MvnI CGCG 2 cut(s) 219, 1011
NaeI GCCGGC 1 cut(s) 12
NarI GGCGCC 1 cut(s) 471
NciI CCSGG 3 cut(s) 245, 351, 500
NdeII GATC 5 cut(s) 484, 730, 829, 844, 1006
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 1 cut(s) 960
NlaIV GGNNCC 5 cut(s) 248, 319, 472, 504, 894
NmuCI GTSAC 1 cut(s) 719
NspV TTCGAA 1 cut(s) 588
PaqCI CACCTGC 1 cut(s) 670
PceI AGGCCT 1 cut(s) 452
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PdiI GCCGGC 1 cut(s) 12
PflFI GACNNNGTC 1 cut(s) 284
PfoI TCCNGGA 2 cut(s) 381, 613
PleI GAGTC 1 cut(s) 178
PluTI GGCGCC 1 cut(s) 474
PpsI GAGTC 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 928
Psp5II RGGWCCY 1 cut(s) 928
Psp6I CCWGG 3 cut(s) 381, 493, 613
PspGI CCWGG 3 cut(s) 381, 493, 613
PspN4I GGNNCC 5 cut(s) 248, 319, 472, 504, 894
PspPI GGNCC 5 cut(s) 193, 247, 497, 892, 928
PspPPI RGGWCCY 1 cut(s) 928
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 792
SaqAI TTAA 4 cut(s) 114, 327, 687, 1165
Sau3AI GATC 5 cut(s) 484, 730, 829, 844, 1006
Sau96I GGNCC 5 cut(s) 193, 247, 497, 892, 928
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 6 cut(s) 245, 351, 383, 495, 500, 615
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 9 cut(s) 152, 375, 547, 586, 598, 796, 843, 1126, 1152
SfoI GGCGCC 1 cut(s) 472
SfuI TTCGAA 1 cut(s) 588
SgrAI CRCCGGYG 1 cut(s) 10
SinI GGWCC 2 cut(s) 892, 928
SmiMI CAYNNNNRTG 1 cut(s) 792
Sse9I AATT 7 cut(s) 574, 725, 883, 909, 965, 994, 1044
SseBI AGGCCT 1 cut(s) 452
SspDI GGCGCC 1 cut(s) 470
SspI AATATT 1 cut(s) 819
SspMI CTAG 1 cut(s) 624
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 6 cut(s) 243, 349, 381, 493, 498, 613
StyI CCWWGG 3 cut(s) 465, 623, 1151
TaaI ACNGT 2 cut(s) 286, 389
TaiI ACGT 2 cut(s) 326, 987
TaqI TCGA 7 cut(s) 174, 255, 344, 588, 790, 1042, 1081
TasI AATT 7 cut(s) 574, 725, 883, 909, 965, 994, 1044
TauI GCSGC 9 cut(s) 17, 252, 296, 308, 357, 360, 476, 479, 518
Tru1I TTAA 4 cut(s) 114, 327, 687, 1165
Tru9I TTAA 4 cut(s) 114, 327, 687, 1165
TscAI CASTG 1 cut(s) 1077
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 3 cut(s) 461, 479, 961
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 1 cut(s) 686
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 1 cut(s) 1077
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 892, 928
XapI RAATTY 2 cut(s) 725, 883
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.