pycom17g14540

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009749
Physical Location & Seq
Reverse (-)
12103885 .. 12107726
3842 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGGCGAAGCAAAGCTCTATCTCCCTCGTTTTCTTTCTTCATGCAGCACTACTAATCTCAACTTGTTTTGGAAGCAAATCTTGGCTTTCAAAAAGGCAAGTTGCCTTGTTCATCTTTGGAGACTCTTACTTAGATTCTGGCAACAACAACTACATCAACACTACAACTCTCGACCAAGCAAATTTCTTGCCGTATGGAGAAACCTACTTCAAGTTCCCAACGGGAAGATTCTCCGACGGACGTTTGATGTCAGACTTCATTGCTGAGTACGCTCACCTGCCATTTGTTCCACCCTTTCTTCAACCGGGATTTCGTCAATATTATGGTGGCGCGAATTTCGCATCCGCCGGAGCTGGTGCTCTTGTTGAGACTTTTCAAGGCGAGGTCATTGATCTTAAGAAACAACTGAAGTACCATAAAAAGGTAGAGACCTGGTTCAGAAATAAGTTAGGCGATGTTGAAGCCAAAGTGACACTTTCAAGAGCCGTCTACTTGTTCAGCATTGGAACCAACGATTACATAAGCCCTTTCCTAACCAATCCTCCCATGTTAAAGTCCTACCCTCGCTCACAATACGTCGGAATGGTCATCGGCAACTTGACAAGCGTTATCCAAGAAATTTATGCAGGAGGGGGTAGGAAATTTGGGTTCATCAACTTGCTGGACTCAGGTTGTCTTCCGGGGTTCAGAATAATCAAGCCTGAAAATAATGGTAGCTGCTTAAAAGAGGTTTCATCACTTGCCAAATTACATAACCAAGCACTCTCAAAACTTCTCATTCAACTTGGAAACCAGTTGGAGGGTTTCAAATATTCACTCTACGACTTGAACAGCAATCTTAGACAGAGAATCAGACATCCCTCTAAATACGGTACGTACGTAAATGCACAAAAGCAAATTAGTTGTCTTAATTCAGTTGATCAGGGTAGGCAGGGTTTTAAAGAAGGGAAAACAGCATGCTGTGGGACAGGTGAATTTAGAGGAGTGTTTAGCTGTGGAGGGAAAAGAATAGTGAATGAGTTTGATTTGTGTGAGAATCCAAATGAATATGTGTTTTGGGACTCGTTGCATCTCACTGAAAAGGTCTACAAGCAATTTGCTAATGAAATGTGGAGCGGCCAGCCATGGAACTCCAAGGCCACAAAAGGTCTACACAATCTCAAGAGTCTATTCCGAGACTTGCTTGACTCATGTTTGACGGTTGGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

404

Amino Acids

45.25

Weight (kDa)

9.14

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 369 4e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 1116
AccI GTMKAC 3 cut(s) 489, 1086, 1150
AccII CGCG 1 cut(s) 332
AciI CCGC 2 cut(s) 345, 1116
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 5 cut(s) 181, 334, 618, 641, 974
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 4 cut(s) 269, 413, 874, 878
AfiI CCNNNNNNNGG 2 cut(s) 421, 799
AflII CTTAAG 1 cut(s) 395
AgsI TTSAA 9 cut(s) 90, 211, 302, 377, 461, 480, 782, 808, 829
AjnI CCWGG 1 cut(s) 431
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AluBI AGCT 4 cut(s) 15, 353, 717, 993
AluI AGCT 4 cut(s) 15, 353, 717, 993
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 4 cut(s) 114, 362, 422, 1170
AoxI GGCC 2 cut(s) 1117, 1137
ApeKI GCWGC 2 cut(s) 44, 717
ApoI RAATTY 5 cut(s) 181, 334, 618, 641, 974
ArsI GACNNNNNNTTYG 2 cut(s) 889, 921
AspLEI GCGC 1 cut(s) 332
AsuC2I CCSGG 2 cut(s) 306, 681
AsuHPI GGTGA 2 cut(s) 266, 983
BaeI ACNNNNGTAYC 4 cut(s) 395, 428, 864, 897
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 2 cut(s) 56, 704
BceAI ACGGC 2 cut(s) 175, 470
BciT130I CCWGG 1 cut(s) 433
BclI TGATCA 1 cut(s) 919
BcnI CCSGG 2 cut(s) 306, 681
BcoDI GTCTC 4 cut(s) 114, 362, 422, 1170
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 3 cut(s) 45, 718, 1117
BlsI GCNGC 3 cut(s) 46, 719, 1118
Bme1390I CCNGG 3 cut(s) 306, 433, 681
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 3 cut(s) 306, 433, 681
BmsI GCATC 2 cut(s) 350, 1078
BpiI GAAGAC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 1145
BpuMI CCSGG 2 cut(s) 306, 681
BsaAI YACGTR 2 cut(s) 876, 880
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 3 cut(s) 680, 1124, 1134
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 799
Bse1I ACTGG 1 cut(s) 793
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 3 cut(s) 680, 1124, 1134
BseGI GGATG 2 cut(s) 341, 856
BseLI CCNNNNNNNGG 2 cut(s) 421, 799
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 255, 681
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 1 cut(s) 996
BseXI GCAGC 2 cut(s) 56, 704
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 1119, 1139
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 3 cut(s) 305, 348, 680
BsiWI CGTACG 1 cut(s) 876
BslFI GGGAC 2 cut(s) 979, 1073
BslI CCNNNNNNNGG 2 cut(s) 421, 799
BsmAI GTCTC 4 cut(s) 114, 362, 422, 1170
BsmFI GGGAC 2 cut(s) 979, 1073
BsnI GGCC 2 cut(s) 1119, 1139
Bso31I GGTCTC 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 2 cut(s) 391, 919
Bsp19I CCATGG 1 cut(s) 1124
BspACI CCGC 2 cut(s) 345, 1116
BspANI GGCC 2 cut(s) 1119, 1139
BspCNI CTCAG 2 cut(s) 256, 680
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 285
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 422
BsrBI CCGCTC 1 cut(s) 1116
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 1 cut(s) 793
BssECI CCNNGG 3 cut(s) 680, 1124, 1134
BssMI GATC 2 cut(s) 391, 919
BssT1I CCWWGG 2 cut(s) 1124, 1134
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 2 cut(s) 872, 1201
BstAFI CTTAAG 1 cut(s) 395
BstBAI YACGTR 2 cut(s) 876, 880
BstC8I GCNNGC 2 cut(s) 958, 1121
BstDEI CTNAG 4 cut(s) 130, 264, 667, 839
BstDSI CCRYGG 1 cut(s) 1124
BstF5I GGATG 2 cut(s) 341, 856
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 2 cut(s) 394, 922
BstMAI GTCTC 4 cut(s) 114, 362, 422, 1170
BstMBI GATC 2 cut(s) 391, 919
BstMWI GCNNNNNNNGC 2 cut(s) 269, 338
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 960
BstSCI CCNGG 3 cut(s) 304, 431, 679
BstSNI TACGTA 2 cut(s) 876, 880
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 56, 704
BstV2I GAAGAC 1 cut(s) 668
BsuRI GGCC 2 cut(s) 1119, 1139
BtgI CCRYGG 1 cut(s) 1124
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 2 cut(s) 341, 856
BtsIMutI CAGTG 1 cut(s) 1074
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 958, 1121
CfoI GCGC 1 cut(s) 332
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 4 cut(s) 268, 412, 873, 877
CviAII CATG 5 cut(s) 41, 547, 957, 1125, 1191
CviQI GTAC 4 cut(s) 268, 412, 873, 877
DdeI CTNAG 4 cut(s) 130, 264, 667, 839
DpnI GATC 2 cut(s) 393, 921
DpnII GATC 2 cut(s) 391, 919
DraI TTTAAA 1 cut(s) 940
EaeI YGGCCR 1 cut(s) 1117
EciI GGCGGA 1 cut(s) 334
Eco105I TACGTA 2 cut(s) 876, 880
Eco130I CCWWGG 2 cut(s) 1124, 1134
Eco31I GGTCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 1124, 1134
ErhI CCWWGG 2 cut(s) 1124, 1134
FaeI CATG 5 cut(s) 44, 550, 960, 1128, 1194
FalI AAGNNNNNCTT 4 cut(s) 64, 96, 459, 491
FaqI GGGAC 2 cut(s) 979, 1073
FatI CATG 5 cut(s) 40, 546, 956, 1124, 1190
FbaI TGATCA 1 cut(s) 919
FblI GTMKAC 3 cut(s) 489, 1086, 1150
Fnu4HI GCNGC 3 cut(s) 45, 718, 1117
FokI GGATG 2 cut(s) 328, 843
Fsp4HI GCNGC 3 cut(s) 45, 718, 1117
GlaI GCGC 1 cut(s) 331
GluI GCNGC 3 cut(s) 45, 718, 1117
HaeIII GGCC 2 cut(s) 1119, 1139
HapII CCGG 3 cut(s) 305, 348, 680
HhaI GCGC 1 cut(s) 332
Hin1II CATG 5 cut(s) 44, 550, 960, 1128, 1194
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 9 cut(s) 122, 134, 228, 665, 849, 1036, 1061, 1165, 1187
HpaII CCGG 3 cut(s) 305, 348, 680
HphI GGTGA 2 cut(s) 266, 983
Hpy166II GTNNAC 3 cut(s) 490, 1087, 1151
Hpy188I TCNGA 7 cut(s) 235, 253, 440, 581, 689, 854, 1175
Hpy188III TCNNGA 3 cut(s) 170, 480, 1162
Hpy8I GTNNAC 3 cut(s) 490, 1087, 1151
Hpy99I CGWCG 2 cut(s) 239, 581
HpyAV CCTTC 1 cut(s) 938
HpyCH4III ACNGT 2 cut(s) 872, 1201
HpyCH4IV ACGT 4 cut(s) 241, 576, 875, 879
HpyCH4V TGCA 4 cut(s) 44, 626, 887, 1069
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 338
HpyF3I CTNAG 4 cut(s) 130, 264, 667, 839
HpySE526I ACGT 4 cut(s) 241, 576, 875, 879
Hsp92II CATG 5 cut(s) 44, 550, 960, 1128, 1194
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 2 cut(s) 391, 919
LmnI GCTCC 2 cut(s) 350, 1113
Lsp1109I GCAGC 2 cut(s) 56, 704
LweI GCATC 2 cut(s) 350, 1078
MabI ACCWGGT 1 cut(s) 431
MaeII ACGT 4 cut(s) 241, 576, 875, 879
MaeIII GTNAC 1 cut(s) 469
MalI GATC 2 cut(s) 393, 921
MbiI CCGCTC 1 cut(s) 1116
MboI GATC 2 cut(s) 391, 919
MboII GAAGA 4 cut(s) 29, 237, 290, 668
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 9 cut(s) 181, 334, 618, 641, 746, 897, 910, 974, 1094
MlyI GAGTC 5 cut(s) 116, 659, 1055, 1174, 1181
MmeI TCCRAC 3 cut(s) 258, 559, 777
MseI TTAA 5 cut(s) 396, 551, 722, 909, 939
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 3 cut(s) 305, 348, 680
MspR9I CCNGG 3 cut(s) 306, 433, 681
MvaI CCWGG 1 cut(s) 433
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 269, 338
NciI CCSGG 2 cut(s) 306, 681
NcoI CCATGG 1 cut(s) 1124
NdeII GATC 2 cut(s) 391, 919
NlaIII CATG 5 cut(s) 44, 550, 960, 1128, 1194
NlaIV GGNNCC 1 cut(s) 508
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 960
PaeI GCATGC 1 cut(s) 960
PaqCI CACCTGC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PfeI GAWTC 4 cut(s) 134, 228, 849, 1036
Pfl23II CGTACG 1 cut(s) 876
PkrI GCNGC 3 cut(s) 46, 719, 1118
PleI GAGTC 5 cut(s) 116, 659, 1055, 1173, 1181
PpsI GAGTC 5 cut(s) 116, 659, 1055, 1173, 1181
Ppu21I YACGTR 2 cut(s) 876, 880
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
PspLI CGTACG 1 cut(s) 876
PspN4I GGNNCC 1 cut(s) 508
RsaI GTAC 4 cut(s) 269, 413, 874, 878
RsaNI GTAC 4 cut(s) 268, 412, 873, 877
SaqAI TTAA 5 cut(s) 396, 551, 722, 909, 939
SatI GCNGC 3 cut(s) 45, 718, 1117
Sau3AI GATC 2 cut(s) 391, 919
SchI GAGTC 5 cut(s) 116, 659, 1055, 1174, 1181
ScrFI CCNGG 3 cut(s) 306, 433, 681
SduI GDGCHC 1 cut(s) 361
SexAI ACCWGGT 1 cut(s) 431
SfaNI GCATC 2 cut(s) 350, 1078
SmlI CTYRAG 2 cut(s) 395, 1160
SmoI CTYRAG 2 cut(s) 395, 1160
SnaBI TACGTA 2 cut(s) 876, 880
SphI GCATGC 1 cut(s) 960
Sse9I AATT 9 cut(s) 181, 334, 618, 641, 746, 897, 910, 974, 1094
SsiI CCGC 2 cut(s) 345, 1116
SspI AATATT 2 cut(s) 320, 812
StyD4I CCNGG 3 cut(s) 304, 431, 679
StyI CCWWGG 2 cut(s) 1124, 1134
TaaI ACNGT 2 cut(s) 872, 1201
TaiI ACGT 4 cut(s) 244, 579, 878, 882
TaqI TCGA 1 cut(s) 171
TasI AATT 9 cut(s) 181, 334, 618, 641, 746, 897, 910, 974, 1094
TauI GCSGC 1 cut(s) 1119
TfiI GAWTC 4 cut(s) 134, 228, 849, 1036
Tru1I TTAA 5 cut(s) 396, 551, 722, 909, 939
Tru9I TTAA 5 cut(s) 396, 551, 722, 909, 939
TscAI CASTG 1 cut(s) 1081
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 2 cut(s) 44, 717
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 7 cut(s) 29, 100, 247, 640, 723, 1059, 1119
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 1081
Vha464I CTTAAG 1 cut(s) 395
XapI RAATTY 5 cut(s) 181, 334, 618, 641, 974
XceI RCATGY 1 cut(s) 960
XmiI GTMKAC 3 cut(s) 489, 1086, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.