pycom17g20180

NUP50 (Nucleoporin 50 kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007109
Physical Location & Seq
Forward (+)
18346660 .. 18347850
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGTTTGTTTCATATTGGCTTCCCAAATACTTGATACACCGGCTGGTTAATCCAATTTTTTTTATTCATTTGTCAATGGCGGATTCGGAAAATGCCCTTCCACTTAATAAGAAGCGAGCTGCTGGGAGGGAACTGTCTCGGGATAACCCAGGCCTTGATGACGAGGACGATGCTTCTGATGCAGAGATTGGAACTTTCAAGAGGGCCAGTGAGGAGGTGCTGGCCACCAGAAGAATTGTGAAGGTTCGTCGCCAGCAGTGTACATCTGCCCCCGCTTCTAATACTTCCAATCCTTTTGCTGGTATACGCTTGGTTCCTCCAACAGGAGCAACTGAAGCCCCTGCTGCAGCTAGCGCACCACCTGCCAGTGAAACACGAGTTTCAGATGAGGATAATAAGCAGCCAGAAAGCAAGGTTGAACAGGCTGATCAAGCTGGGGATGACTCTGCTGCAGATAAGGAGAATGTTGTGAAGGAGAACAATGAAGTTTCCAGTGAGGCAACGGAACAACCTGAACCATCTGTGGGTGAAAATAAGACAAATGAGGGTGATGCAGTAAATGAGGAAGAGGAAAAGGCTGAAATTGAAGATAAGAAAGATGGAGAAGATAAGAAAGATAACAAAAACGAAAATGTTGATCGATCCCTCTCATCTTTAGTCCGGAAGGTAACTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000054 GO:0000056 GO:0000082 GO:0000226 GO:0000278 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006403 GO:0006405 GO:0006508 GO:0006511 GO:0006606 GO:0006611 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0008536 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0017016 GO:0017038 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0022402 GO:0022613 GO:0030163 GO:0031267 GO:0031323 GO:0031326 GO:0031503 GO:0031935 GO:0031938 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033750 GO:0034504 GO:0034613 GO:0042254 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051020 GO:0051128 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051603 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0060255 GO:0060968 GO:0065007 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902275 GO:1903047 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05166 map05166
Pfam Domains
Protein Families
NUP50 Ran_BP1

Protein Analysis

226

Amino Acids

24.68

Weight (kDa)

4.71

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUP50 PF08911 37 - 102 6.4e-17 NUP50 (Nucleoporin 50 kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 370
Acc36I ACCTGC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 304
AccIII TCCGGA 1 cut(s) 660
AciI CCGC 2 cut(s) 80, 273
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 3 cut(s) 299, 323, 524
AgsI TTSAA 3 cut(s) 199, 419, 587
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 3 cut(s) 119, 350, 434
AluI AGCT 3 cut(s) 119, 350, 434
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 1 cut(s) 636
Ama87I CYCGRG 1 cut(s) 138
Aor13HI TCCGGA 1 cut(s) 660
AoxI GGCC 3 cut(s) 151, 204, 222
ApeKI GCWGC 5 cut(s) 119, 344, 347, 400, 449
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 2 cut(s) 539, 560
AsuNHI GCTAGC 1 cut(s) 350
AvaI CYCGRG 1 cut(s) 138
BalI TGGCCA 1 cut(s) 224
BauI CACGAG 1 cut(s) 375
BbvI GCAGC 5 cut(s) 106, 331, 359, 412, 436
BccI CCATC 2 cut(s) 526, 593
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BciT130I CCWGG 1 cut(s) 150
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 2 cut(s) 345, 450
BfuAI ACCTGC 1 cut(s) 370
BisI GCNGC 5 cut(s) 120, 345, 348, 401, 450
BlsI GCNGC 5 cut(s) 121, 346, 349, 402, 451
Bme1390I CCNGG 1 cut(s) 150
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 3 cut(s) 160, 169, 541
BmtI GCTAGC 1 cut(s) 354
Bsa29I ATCGAT 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 148
BsaWI WCCGGW 1 cut(s) 660
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 323, 524
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 3 cut(s) 207, 366, 492
BseAI TCCGGA 1 cut(s) 660
BseBI CCWGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 640
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 3 cut(s) 299, 323, 524
BseNI ACTGG 3 cut(s) 207, 366, 492
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 5 cut(s) 106, 331, 359, 412, 436
BseYI CCCAGC 2 cut(s) 122, 434
BshFI GGCC 3 cut(s) 153, 206, 224
BshVI ATCGAT 1 cut(s) 640
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 2 cut(s) 40, 661
BslI CCNNNNNNNGG 3 cut(s) 299, 323, 524
BsmAI GTCTC 1 cut(s) 141
BsnI GGCC 3 cut(s) 153, 206, 224
BsoBI CYCGRG 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 660
Bsp1407I TGTACA 1 cut(s) 260
Bsp143I GATC 3 cut(s) 427, 637, 641
BspACI CCGC 2 cut(s) 80, 273
BspANI GGCC 3 cut(s) 153, 206, 224
BspDI ATCGAT 1 cut(s) 640
BspEI TCCGGA 1 cut(s) 660
BspLI GGNNCC 1 cut(s) 315
BspMAI CTGCAG 2 cut(s) 349, 454
BspMI ACCTGC 1 cut(s) 370
BspOI GCTAGC 1 cut(s) 354
BspPI GGATC 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 260
BsrI ACTGG 3 cut(s) 207, 366, 492
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 3 cut(s) 427, 637, 641
BssNAI GTATAC 1 cut(s) 305
BssSI CACGAG 1 cut(s) 375
Bst1107I GTATAC 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 561
BstAPI GCANNNNNTGC 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 260
BstC8I GCNNGC 4 cut(s) 117, 222, 254, 352
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 3 cut(s) 430, 640, 644
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 3 cut(s) 427, 637, 641
BstMWI GCNNNNNNNGC 6 cut(s) 179, 335, 344, 353, 362, 431
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 2 cut(s) 345, 450
BstV1I GCAGC 5 cut(s) 106, 331, 359, 412, 436
BstZ17I GTATAC 1 cut(s) 305
Bsu15I ATCGAT 1 cut(s) 640
BsuRI GGCC 3 cut(s) 153, 206, 224
BsuTUI ATCGAT 1 cut(s) 640
BtsCI GGATG 1 cut(s) 445
BtsI GCAGTG 1 cut(s) 263
BtsIMutI CAGTG 4 cut(s) 214, 263, 373, 499
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 4 cut(s) 117, 222, 254, 352
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 640
Csp6I GTAC 1 cut(s) 261
CviQI GTAC 1 cut(s) 261
DpnI GATC 3 cut(s) 429, 639, 643
DpnII GATC 3 cut(s) 427, 637, 641
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 95
Eco147I AGGCCT 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 148
FaiI YATR 2 cut(s) 13, 305
FauI CCCGC 1 cut(s) 280
FbaI TGATCA 1 cut(s) 427
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 5 cut(s) 120, 345, 348, 401, 450
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 5 cut(s) 120, 345, 348, 401, 450
FspBI CTAG 1 cut(s) 351
GlaI GCGC 1 cut(s) 355
GluI GCNGC 5 cut(s) 120, 345, 348, 401, 450
GsaI CCCAGC 2 cut(s) 126, 438
HaeIII GGCC 3 cut(s) 153, 206, 224
HapII CCGG 2 cut(s) 40, 661
HhaI GCGC 1 cut(s) 356
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HinfI GANTC 2 cut(s) 83, 443
HpaII CCGG 2 cut(s) 40, 661
HphI GGTGA 2 cut(s) 539, 560
Hpy166II GTNNAC 2 cut(s) 261, 305
Hpy188I TCNGA 3 cut(s) 88, 178, 385
Hpy188III TCNNGA 3 cut(s) 140, 199, 661
Hpy8I GTNNAC 2 cut(s) 261, 305
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 4 cut(s) 107, 235, 466, 658
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 182, 347, 452, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 335, 344, 353, 362, 431
HspAI GCGC 1 cut(s) 354
Kpn2I TCCGGA 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 3 cut(s) 427, 637, 641
LmnI GCTCC 1 cut(s) 326
Lsp1109I GCAGC 5 cut(s) 106, 331, 359, 412, 436
LweI GCATC 3 cut(s) 160, 169, 541
MaeI CTAG 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 667
MalI GATC 3 cut(s) 429, 639, 643
MboI GATC 3 cut(s) 427, 637, 641
MboII GAAGA 4 cut(s) 243, 578, 599, 617
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 3 cut(s) 54, 234, 582
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 344
Mox20I TGGCCA 1 cut(s) 224
MroI TCCGGA 1 cut(s) 660
MscI TGGCCA 1 cut(s) 224
MseI TTAA 2 cut(s) 48, 105
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 2 cut(s) 40, 661
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 6 cut(s) 179, 335, 344, 353, 362, 431
NdeII GATC 3 cut(s) 427, 637, 641
NheI GCTAGC 1 cut(s) 350
NlaIV GGNNCC 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 370
PceI AGGCCT 1 cut(s) 153
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 5 cut(s) 121, 346, 349, 402, 451
PleI GAGTC 1 cut(s) 437
PpsI GAGTC 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 148
PspFI CCCAGC 2 cut(s) 122, 434
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 1 cut(s) 204
PstI CTGCAG 2 cut(s) 349, 454
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 48, 105
SatI GCNGC 5 cut(s) 120, 345, 348, 401, 450
Sau3AI GATC 3 cut(s) 427, 637, 641
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 150
SetI ASST 9 cut(s) 121, 219, 246, 352, 364, 417, 436, 514, 669
SfaNI GCATC 3 cut(s) 160, 169, 541
SfcI CTRYAG 2 cut(s) 345, 450
Sse9I AATT 3 cut(s) 54, 234, 582
SseBI AGGCCT 1 cut(s) 153
SsiI CCGC 2 cut(s) 80, 273
SspMI CTAG 1 cut(s) 351
StuI AGGCCT 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 640
TasI AATT 3 cut(s) 54, 234, 582
TatI WGTACW 1 cut(s) 260
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 48, 105
Tru9I TTAA 2 cut(s) 48, 105
TscAI CASTG 4 cut(s) 214, 263, 373, 499
TseI GCWGC 5 cut(s) 119, 344, 347, 400, 449
TspDTI ATGAA 2 cut(s) 56, 498
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 4 cut(s) 214, 263, 373, 499
XagI CCTNNNNNAGG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.