pycom17g20290

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005617
Physical Location & Seq
Reverse (-)
18460183 .. 18463077
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1311 bp
ATGGATGTTCCCTCTGCAGAACCAAATAGTGACACCCAAGGTGACCAATTTCCTTTGCTACTAGAGCAGATGGAAAGTCATAACAATCGTGTGCACGTAATTGATGTAACAAGGAATGATGATGCTTCATCAAGTACATCTAGTGATGACCAACCTCCTAGAGTGGACATGCCCCAGCATGAAGACAGATCATCAGCAAGTACACATGCCCCCACTTATCAAGCTGCTTCCTCAAACAGATTGAATTCTAGGAATTCCTCATTTATGAGGAGAGGTGATGGGTATAGTCGTCGCCGAAGAAGCCCTTTAAATTCAGGGTTATGGATTGCTGTTGAGCTTGTTGTCACTGTGAGTCAGATTATAGCATCTATTGTTGTTTTGTCACTGTCAACAAATGAAAATCCACAAGCCCCTTTGTTTGCATGGGTTGTGGGTTATGCATCAGGTTGTGTTGCAACACTTCCTGTTCTTTACTGGAGGTTCCGTAACCGCAATAGGGGTTCTGAGCAAGAATCCATTCAATCGCATCAAGGGTCTTCTGAAAGCAGTCCTCCTGAACCAACGTCTTATACAGCTATTTCAATTACACAAGCTTCTGATGAAGAGCACGGTCGCACCTCCGAAACAGTTACAACTAACCCTCAAATTGGAGGACCCTTAAGTGCAAGATGGTGTCATGAAGTGAGGAAGCTCATTCAATTTTGCAGAATTAATGGGTTAGTGGATCATTTCAAGATGGCCTTAGATTGCTTCTTTGCAGTCTGGTTTGTTGTAGGCAATGTGTGGATTTTTGGAGGTCACTCTGTCCCTTCTGATGCTCCTAAACTGTACAGGTTATGTATAGTGTTCCTTACCTTTAGCTGTATTGGATATGCCATGCCATTCATACTATGTGCAACAATTTGCTGCTGCCTGCCTTGCATCATTTCTGTCCTTGGATTTCGAGAGGATCTTTCTCAGGCTAGAGGAGCCACTGTGGAATCAATTAATGCTCTCCCGACATACAAATTCAAGTTAAAGGAAAATGTGACCAATGATGATCAGGAGGGCAGCCCAGGTAGTGAAGGTGGGCTCTTGGCTGCAGGGACAGAAAAGGAGCGTGAGATATCTGGAGATGATGCGGTTTGTTGTATTTGCTTGGCGAAGTATGCAGATGACGATGAGCTACGGGAGCTACCATGTTTACATGTCTTTCACGTGGAGTGTGTTGACAAATGGTTGAAAATCAATGCATCATGTCCCCTATGCAAAAGCGAGGTCGGAGAGAGCAGCGGTGGCTCGCCATTGGCGAGGGACTCCAGCCAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

437

Amino Acids

47.87

Weight (kDa)

5.21

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 376 - 417 8.3e-14 Ring finger domain
zf-RING_11 PF17123 376 - 403 2.2e-08 RING-like zinc finger
zf-rbx1 PF12678 376 - 417 2.2e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 376 - 416 3.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 376 - 416 6.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 490, 1121, 1272
AclWI GGATC 2 cut(s) 732, 957
AcsI RAATTY 4 cut(s) 244, 253, 310, 1007
AcvI CACGTG 1 cut(s) 1198
AfaI GTAC 3 cut(s) 136, 202, 830
AfiI CCNNNNNNNGG 2 cut(s) 496, 647
AflII CTTAAG 1 cut(s) 658
AflIII ACRYGT 1 cut(s) 1186
AgsI TTSAA 7 cut(s) 244, 521, 582, 698, 733, 1012, 1222
AjnI CCWGG 1 cut(s) 1054
AluBI AGCT 8 cut(s) 224, 337, 575, 593, 691, 861, 1165, 1174
AluI AGCT 8 cut(s) 224, 337, 575, 593, 691, 861, 1165, 1174
Alw21I GWGCWC 2 cut(s) 96, 609
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 2 cut(s) 732, 957
AoxI GGCC 1 cut(s) 738
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 6 cut(s) 224, 906, 909, 1050, 1079, 1269
ApoI RAATTY 4 cut(s) 244, 253, 310, 1007
ArsI GACNNNNNNTTYG 2 cut(s) 1242, 1274
AseI ATTAAT 2 cut(s) 711, 987
Asp700I GAANNNNTTC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 653
AsuHPI GGTGA 2 cut(s) 53, 287
AvaII GGWCC 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 1074
BbrPI CACGTG 1 cut(s) 1198
BbsI GAAGAC 2 cut(s) 189, 528
Bbv12I GWGCWC 2 cut(s) 96, 609
BbvI GCAGC 6 cut(s) 211, 893, 896, 1062, 1066, 1281
BccI CCATC 4 cut(s) 64, 272, 663, 730
BciT130I CCWGG 1 cut(s) 1056
BclI TGATCA 1 cut(s) 1039
BfaI CTAG 5 cut(s) 62, 141, 159, 249, 963
BfmI CTRYAG 2 cut(s) 15, 1080
BfrI CTTAAG 1 cut(s) 658
BisI GCNGC 6 cut(s) 225, 907, 910, 1051, 1080, 1270
BlsI GCNGC 6 cut(s) 226, 908, 911, 1052, 1081, 1271
Bme1390I CCNGG 1 cut(s) 1056
Bme18I GGWCC 1 cut(s) 653
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 3 cut(s) 482, 655, 970
BmrFI CCNGG 1 cut(s) 1056
BmsI GCATC 8 cut(s) 112, 374, 449, 535, 805, 930, 1108, 1241
BpiI GAAGAC 2 cut(s) 189, 528
BpmI CTGGAG 3 cut(s) 496, 1131, 1282
BsaAI YACGTR 2 cut(s) 97, 1198
BsaJI CCNNGG 3 cut(s) 37, 934, 1054
BsaXI ACNNNNNCTCC 4 cut(s) 469, 499, 960, 990
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 647
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 784
BseBI CCWGG 1 cut(s) 1056
BseDI CCNNGG 3 cut(s) 37, 934, 1054
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 496, 647
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 2 cut(s) 495, 971
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 2 cut(s) 283, 981
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 6 cut(s) 211, 893, 896, 1062, 1066, 1281
BseYI CCCAGC 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 613
BshFI GGCC 1 cut(s) 740
BsiEI CGRYCG 1 cut(s) 613
BsiHKAI GWGCWC 2 cut(s) 96, 609
BslFI GGGAC 4 cut(s) 791, 1099, 1224, 1307
BslI CCNNNNNNNGG 2 cut(s) 496, 647
BsmFI GGGAC 4 cut(s) 791, 1099, 1224, 1307
BsnI GGCC 1 cut(s) 740
Bsp1286I GDGCHC 3 cut(s) 96, 609, 1074
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 4 cut(s) 188, 724, 949, 1039
BspACI CCGC 3 cut(s) 490, 1121, 1272
BspANI GGCC 1 cut(s) 740
BspCNI CTCAG 2 cut(s) 496, 970
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 482, 655, 970
BspMAI CTGCAG 2 cut(s) 19, 1084
BspPI GGATC 2 cut(s) 732, 957
BspQI GCTCTTC 1 cut(s) 597
BspTI CTTAAG 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 784
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 37, 934, 1054
BssMI GATC 4 cut(s) 188, 724, 949, 1039
BssT1I CCWWGG 2 cut(s) 37, 934
Bst2UI CCWGG 1 cut(s) 1056
Bst4CI ACNGT 7 cut(s) 349, 387, 611, 628, 828, 976, 1308
Bst6I CTCTTC 1 cut(s) 597
BstAFI CTTAAG 1 cut(s) 658
BstAUI TGTACA 1 cut(s) 828
BstBAI YACGTR 2 cut(s) 97, 1198
BstC8I GCNNGC 2 cut(s) 914, 1280
BstDEI CTNAG 3 cut(s) 504, 742, 957
BstEII GGTNACC 1 cut(s) 41
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 4 cut(s) 191, 727, 952, 1042
BstMBI GATC 4 cut(s) 188, 724, 949, 1039
BstMCI CGRYCG 1 cut(s) 613
BstMWI GCNNNNNNNGC 7 cut(s) 64, 300, 918, 968, 1148, 1171, 1275
BstNI CCWGG 1 cut(s) 1056
BstNSI RCATGY 3 cut(s) 172, 209, 1190
BstPI GGTNACC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 1054
BstSFI CTRYAG 2 cut(s) 15, 1080
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 6 cut(s) 211, 893, 896, 1062, 1066, 1281
BstV2I GAAGAC 2 cut(s) 189, 528
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BsuRI GGCC 1 cut(s) 740
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 3 cut(s) 345, 383, 972
Cac8I GCNNGC 2 cut(s) 914, 1280
CciI TCATGA 1 cut(s) 676
Cfr13I GGNCC 1 cut(s) 653
Csp6I GTAC 3 cut(s) 135, 201, 829
CviAII CATG 9 cut(s) 169, 179, 206, 423, 677, 877, 1179, 1187, 1236
CviQI GTAC 3 cut(s) 135, 201, 829
DdeI CTNAG 3 cut(s) 504, 742, 957
DpnI GATC 4 cut(s) 190, 726, 951, 1041
DpnII GATC 4 cut(s) 188, 724, 949, 1039
DraI TTTAAA 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Eco130I CCWWGG 2 cut(s) 37, 934
Eco24I GRGCYC 1 cut(s) 1074
Eco32I GATATC 1 cut(s) 1107
Eco47I GGWCC 1 cut(s) 653
Eco72I CACGTG 1 cut(s) 1198
Eco91I GGTNACC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 653
EcoO65I GGTNACC 1 cut(s) 41
EcoRI GAATTC 2 cut(s) 244, 253
EcoRII CCWGG 1 cut(s) 1054
EcoRV GATATC 1 cut(s) 1107
EcoT14I CCWWGG 2 cut(s) 37, 934
EcoT22I ATGCAT 2 cut(s) 442, 1234
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 2 cut(s) 37, 934
FaeI CATG 9 cut(s) 172, 182, 209, 426, 680, 880, 1182, 1190, 1239
FalI AAGNNNNNCTT 4 cut(s) 289, 321, 725, 757
FaqI GGGAC 4 cut(s) 791, 1099, 1224, 1307
FatI CATG 9 cut(s) 168, 178, 205, 422, 676, 876, 1178, 1186, 1235
FbaI TGATCA 1 cut(s) 1039
Fnu4HI GCNGC 6 cut(s) 225, 907, 910, 1051, 1080, 1270
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 6 cut(s) 225, 907, 910, 1051, 1080, 1270
FspBI CTAG 5 cut(s) 62, 141, 159, 249, 963
GluI GCNGC 6 cut(s) 225, 907, 910, 1051, 1080, 1270
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 3 cut(s) 496, 1131, 1282
HaeIII GGCC 1 cut(s) 740
Hin1II CATG 9 cut(s) 172, 182, 209, 426, 680, 880, 1182, 1190, 1239
HincII GTYRAC 2 cut(s) 390, 1210
HindII GTYRAC 2 cut(s) 390, 1210
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 352, 512, 980, 1295
HphI GGTGA 2 cut(s) 53, 287
Hpy166II GTNNAC 6 cut(s) 94, 166, 203, 390, 1184, 1210
Hpy188I TCNGA 7 cut(s) 357, 505, 541, 598, 622, 814, 1262
Hpy188III TCNNGA 7 cut(s) 554, 677, 733, 944, 997, 1043, 1110
Hpy8I GTNNAC 6 cut(s) 94, 166, 203, 390, 1184, 1210
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 819, 1058
HpyCH4III ACNGT 7 cut(s) 349, 387, 611, 628, 828, 976, 1308
HpyCH4IV ACGT 3 cut(s) 96, 563, 1197
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 300, 918, 968, 1148, 1171, 1275
HpyF3I CTNAG 3 cut(s) 504, 742, 957
HpySE526I ACGT 3 cut(s) 96, 563, 1197
Hsp92II CATG 9 cut(s) 172, 182, 209, 426, 680, 880, 1182, 1190, 1239
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 4 cut(s) 188, 724, 949, 1039
LguI GCTCTTC 1 cut(s) 597
LmnI GCTCC 4 cut(s) 823, 968, 1096, 1171
Lsp1109I GCAGC 6 cut(s) 211, 893, 896, 1062, 1066, 1281
LweI GCATC 8 cut(s) 112, 374, 449, 535, 805, 930, 1108, 1241
MaeI CTAG 5 cut(s) 62, 141, 159, 249, 963
MaeII ACGT 3 cut(s) 96, 563, 1197
MaeIII GTNAC 9 cut(s) 29, 41, 106, 343, 381, 485, 628, 797, 1027
MalI GATC 4 cut(s) 190, 726, 951, 1041
MboI GATC 4 cut(s) 188, 724, 949, 1039
MboII GAAGA 4 cut(s) 194, 309, 528, 614
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 3 cut(s) 96, 609, 1074
MlyI GAGTC 2 cut(s) 361, 1289
MmeI TCCRAC 1 cut(s) 1240
Mph1103I ATGCAT 2 cut(s) 442, 1234
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 5 cut(s) 308, 659, 711, 987, 1016
MspA1I CMGCKG 1 cut(s) 1272
MspCI CTTAAG 1 cut(s) 658
MspR9I CCNGG 1 cut(s) 1056
MvaI CCWGG 1 cut(s) 1056
MwoI GCNNNNNNNGC 7 cut(s) 64, 300, 918, 968, 1148, 1171, 1275
NdeII GATC 4 cut(s) 188, 724, 949, 1039
NlaIII CATG 9 cut(s) 172, 182, 209, 426, 680, 880, 1182, 1190, 1239
NlaIV GGNNCC 3 cut(s) 482, 655, 970
NmuCI GTSAC 6 cut(s) 29, 41, 343, 381, 797, 1027
NsiI ATGCAT 2 cut(s) 442, 1234
NspI RCATGY 3 cut(s) 172, 209, 1190
PagI TCATGA 1 cut(s) 676
PciI ACATGT 1 cut(s) 1186
PciSI GCTCTTC 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 1286
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 512, 980
PkrI GCNGC 6 cut(s) 226, 908, 911, 1052, 1081, 1271
PleI GAGTC 2 cut(s) 360, 1289
PmaCI CACGTG 1 cut(s) 1198
PmlI CACGTG 1 cut(s) 1198
PpsI GAGTC 2 cut(s) 360, 1289
Ppu21I YACGTR 2 cut(s) 97, 1198
PpuMI RGGWCCY 1 cut(s) 653
PscI ACATGT 1 cut(s) 1186
PshBI ATTAAT 2 cut(s) 711, 987
Psp5II RGGWCCY 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 1054
PspCI CACGTG 1 cut(s) 1198
PspEI GGTNACC 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 1054
PspN4I GGNNCC 3 cut(s) 482, 655, 970
PspPI GGNCC 1 cut(s) 653
PspPPI RGGWCCY 1 cut(s) 653
PstI CTGCAG 2 cut(s) 19, 1084
PsuI RGATCY 1 cut(s) 949
RsaI GTAC 3 cut(s) 136, 202, 830
RsaNI GTAC 3 cut(s) 135, 201, 829
SapI GCTCTTC 1 cut(s) 597
SaqAI TTAA 5 cut(s) 308, 659, 711, 987, 1016
SatI GCNGC 6 cut(s) 225, 907, 910, 1051, 1080, 1270
Sau3AI GATC 4 cut(s) 188, 724, 949, 1039
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 2 cut(s) 361, 1289
ScrFI CCNGG 1 cut(s) 1056
SduI GDGCHC 3 cut(s) 96, 609, 1074
SfaNI GCATC 8 cut(s) 112, 374, 449, 535, 805, 930, 1108, 1241
SfcI CTRYAG 2 cut(s) 15, 1080
SinI GGWCC 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 658
SmoI CTYRAG 1 cut(s) 658
SsiI CCGC 3 cut(s) 490, 1121, 1272
SspMI CTAG 5 cut(s) 62, 141, 159, 249, 963
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 2 cut(s) 37, 934
TaaI ACNGT 7 cut(s) 349, 387, 611, 628, 828, 976, 1308
TaiI ACGT 3 cut(s) 99, 566, 1200
TaqI TCGA 1 cut(s) 943
TatI WGTACW 3 cut(s) 134, 200, 828
TfiI GAWTC 2 cut(s) 512, 980
Tru1I TTAA 5 cut(s) 308, 659, 711, 987, 1016
Tru9I TTAA 5 cut(s) 308, 659, 711, 987, 1016
TscAI CASTG 3 cut(s) 352, 390, 979
TseFI GTSAC 6 cut(s) 29, 41, 343, 381, 797, 1027
TseI GCWGC 6 cut(s) 224, 906, 909, 1050, 1079, 1269
Tsp45I GTSAC 6 cut(s) 29, 41, 343, 381, 797, 1027
TspDTI ATGAA 6 cut(s) 117, 195, 411, 615, 693, 874
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 3 cut(s) 352, 390, 979
Vha464I CTTAAG 1 cut(s) 658
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 653
VspI ATTAAT 2 cut(s) 711, 987
XapI RAATTY 4 cut(s) 244, 253, 310, 1007
XceI RCATGY 3 cut(s) 172, 209, 1190
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 5 cut(s) 62, 141, 159, 249, 963
Zsp2I ATGCAT 2 cut(s) 442, 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.