pycom17g21950

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005648
Physical Location & Seq
Reverse (-)
20357514 .. 20361282
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1191 bp
ATGCCGGAGCTACCGGAGGTGGAGGCGGCTCGGAGGGCCGTAGAGGAACACTGCGTAGGGAAGAAGATAACGAAAGCACTCATCGCCGACGACTCCAAAGTCATCGATGGAGTCTCACGCGCCGACTTCGAGGCCTCTCTCTTGGGAAAGACCATCGTCTCCGCTCACCGCAAGGGCAAGAATATGTGGCTCTGCCTCGACTCTCCTCCCTTTCCTTCCTTCCAATTCGGAATGGCGGGTGCTGTATATATTAAAGGGGTCGCCGTCACGAAATATAAGAGGTCTGCTGTAAAGGACACTGATGAGTGGCCTTCCAAGTATTCCAAGTTCTTTGTTGAATTGGATGATGGTCTGGAGTTCTCTTTCACTGACAAGAGGCGATTTGCTAGAGTCCGCTTGCTGAAAGATCCAACTTCTGTGCCCCCCATATCTGAGCTTGGGCCCGATGCTCTCTTGGAGCCTATGACAGTGGATGAACTTTTTGAATGCTTCAGCAAGAAGAAAATTGCAATTAAGACCCTGTTGCTTGATCAGAGTGTTATTTCTGGAATTGGCAATTGGGTTGCTGATGAAGTACTCTACCAAGCAAGAATTCATCCGGAGCAAGGTGCTGCGAGCCTGTCGAAAGAAAATTGTGCAGCTTTACACAAGAGCATTAAAGAGGTACTTGAAAAATCCCTTGAAGTTGGAGCAGATAGTAGTCAATTTCCTAGTGATTGGATTTTTCATTCCCGTGAAAAGAAGCCTGGAAAGGCTTTTGTTGATGGGAAGAAAATTGAGTTTATCACAGTTGGTAGCAGGACTTCAGCATTTGTACCAGAGCTTCAGAAGTTAAGTGGACCGCAAGCTTCCAAAGCAGCAAGTAAACAAGCTAACAAGAAAAAAGGGCGTGGTGATGTTGTTGATGAAGCAGCAAGTGATGAAGAAGCCAATGAAAATGAAAAATCGAAGAAGGGGAGGAATCCTAAAGGTCAAGGTAAGAGGGCGTCAGCGAAAAGAAAATCGAAAGAGAGTGATGATGAAGACAAGGACAGTGATGATGATGCTGACGACGATGATGACCATCAGGATGAAGATCAAAAGAAGAAATCAAGAAAAGGTGTCAATAATAAGCAATCCAAGGCAGAGAAGACATCAAAGGCAAAGGTGCAGACCAGTAAAAGTGGTAAGAAGCTGAAGAAAGGAAAATAG

Protein Analysis

397

Amino Acids

43.69

Weight (kDa)

9.03

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 2.5e-28 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 236 2.4e-31 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 240 - 273 4.2e-22 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 3 cut(s) 475, 789, 809
AcyI GRCGYC 1 cut(s) 986
AfaI GTAC 3 cut(s) 576, 666, 816
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 4 cut(s) 338, 485, 671, 683
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AluBI AGCT 7 cut(s) 10, 436, 641, 823, 848, 872, 1174
AluI AGCT 7 cut(s) 10, 436, 641, 823, 848, 872, 1174
Alw26I GTCTC 2 cut(s) 118, 163
AlwI GGATC 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 4 cut(s) 36, 132, 308, 440
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 611, 638, 857, 911
ApoI RAATTY 1 cut(s) 591
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 4 cut(s) 36, 440, 441, 839
AsuHPI GGTGA 2 cut(s) 158, 905
AvaII GGWCC 1 cut(s) 839
BaeGI GKGCMC 2 cut(s) 423, 444
BanII GRGCYC 1 cut(s) 444
BarI GAAGNNNNNNTAC 4 cut(s) 787, 807, 819, 839
BbsI GAAGAC 2 cut(s) 1029, 1136
BbvI GCAGC 4 cut(s) 598, 650, 869, 923
BccI CCATC 5 cut(s) 101, 161, 341, 758, 1071
BceAI ACGGC 2 cut(s) 23, 248
BciT130I CCWGG 1 cut(s) 747
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 118, 163
BfaI CTAG 2 cut(s) 387, 711
BisI GCNGC 5 cut(s) 27, 612, 639, 858, 912
BlsI GCNGC 5 cut(s) 28, 613, 640, 859, 913
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 839
BmgT120I GGNCC 4 cut(s) 36, 440, 441, 839
BmiI GGNNCC 2 cut(s) 442, 459
BmrFI CCNGG 1 cut(s) 747
BmsI GCATC 2 cut(s) 436, 1033
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 1029, 1136
BpmI CTGGAG 1 cut(s) 374
Bsa29I ATCGAT 1 cut(s) 105
BsaBI GATNNNNATC 2 cut(s) 1062, 1074
BsaHI GRCGYC 1 cut(s) 986
BsaJI CCNNGG 1 cut(s) 1119
BsaWI WCCGGW 2 cut(s) 13, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse1I ACTGG 1 cut(s) 1155
Bse8I GATNNNNATC 2 cut(s) 1062, 1074
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 1 cut(s) 747
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 1119
BseGI GGATG 4 cut(s) 349, 478, 595, 1075
BseJI GATNNNNATC 2 cut(s) 1062, 1074
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 1155
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 423, 444
BseXI GCAGC 4 cut(s) 598, 650, 869, 923
BsgI GTGCAG 2 cut(s) 657, 1169
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 38, 134, 310, 442
BshVI ATCGAT 1 cut(s) 105
BsiSI CCGG 3 cut(s) 5, 14, 599
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 2 cut(s) 118, 163
BsmBI CGTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 4 cut(s) 38, 134, 310, 442
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 423, 444
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 3 cut(s) 406, 529, 1075
BspACI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
BspANI GGCC 4 cut(s) 38, 134, 310, 442
BspCNI CTCAG 1 cut(s) 424
BspDI ATCGAT 1 cut(s) 105
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 442, 459
BspPI GGATC 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 1155
BssECI CCNNGG 1 cut(s) 1119
BssMI GATC 3 cut(s) 406, 529, 1075
BssNI GRCGYC 1 cut(s) 986
BssT1I CCWWGG 1 cut(s) 1119
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 3 cut(s) 469, 790, 1034
BstACI GRCGYC 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 398, 616, 846
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 4 cut(s) 349, 478, 595, 1075
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 3 cut(s) 409, 532, 1078
BstMAI GTCTC 2 cut(s) 118, 163
BstMBI GATC 3 cut(s) 406, 529, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 35, 83, 854
BstNI CCWGG 1 cut(s) 747
BstPAI GACNNNNGTC 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 745
BstSLI GKGCMC 2 cut(s) 423, 444
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 598, 650, 869, 923
BstV2I GAAGAC 2 cut(s) 1029, 1136
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 4 cut(s) 38, 134, 310, 442
BsuTUI ATCGAT 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 349, 478, 595, 1075
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 5 cut(s) 49, 297, 366, 474, 1039
Cac8I GCNNGC 3 cut(s) 398, 616, 846
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 36, 440, 441, 839
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 1 cut(s) 975
Csp6I GTAC 3 cut(s) 575, 665, 815
CviQI GTAC 3 cut(s) 575, 665, 815
DdeI CTNAG 1 cut(s) 432
DpnI GATC 3 cut(s) 408, 531, 1077
DpnII GATC 3 cut(s) 406, 529, 1075
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 1119
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 839
Eco57I CTGAAG 3 cut(s) 475, 789, 809
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 1119
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 1119
Esp3I CGTCTC 1 cut(s) 163
FaiI YATR 6 cut(s) 185, 247, 249, 276, 428, 464
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 5 cut(s) 27, 612, 639, 858, 912
FokI GGATG 4 cut(s) 356, 485, 582, 1082
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 5 cut(s) 27, 612, 639, 858, 912
FspBI CTAG 2 cut(s) 387, 711
GlaI GCGC 1 cut(s) 121
GluI GCNGC 5 cut(s) 27, 612, 639, 858, 912
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 4 cut(s) 38, 134, 310, 442
HapII CCGG 3 cut(s) 5, 14, 599
HgaI GACGC 1 cut(s) 975
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 986
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HindIII AAGCTT 1 cut(s) 846
HinfI GANTC 5 cut(s) 92, 111, 200, 390, 961
HpaII CCGG 3 cut(s) 5, 14, 599
HphI GGTGA 2 cut(s) 158, 905
Hpy166II GTNNAC 2 cut(s) 839, 866
Hpy188I TCNGA 5 cut(s) 33, 230, 433, 534, 828
Hpy188III TCNNGA 6 cut(s) 268, 353, 546, 599, 1067, 1092
Hpy8I GTNNAC 2 cut(s) 839, 866
Hpy99I CGWCG 2 cut(s) 92, 1055
HpyAV CCTTC 4 cut(s) 225, 229, 321, 946
HpyCH4III ACNGT 3 cut(s) 469, 790, 1034
HpyCH4V TGCA 3 cut(s) 509, 638, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 83, 854
HpyF3I CTNAG 1 cut(s) 432
Hsp92I GRCGYC 1 cut(s) 986
HspAI GCGC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 406, 529, 1075
LmnI GCTCC 4 cut(s) 7, 457, 601, 689
Lsp1109I GCAGC 4 cut(s) 598, 650, 869, 923
LweI GCATC 2 cut(s) 436, 1033
MaeI CTAG 2 cut(s) 387, 711
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 408, 531, 1077
MbiI CCGCTC 1 cut(s) 164
MboI GATC 3 cut(s) 406, 529, 1075
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 423, 444
MlyI GAGTC 4 cut(s) 86, 120, 194, 399
MmeI TCCRAC 2 cut(s) 434, 667
MroI TCCGGA 1 cut(s) 598
MseI TTAA 4 cut(s) 252, 513, 657, 833
MslI CAYNNNNRTG 2 cut(s) 732, 1068
MspI CCGG 3 cut(s) 5, 14, 599
MspR9I CCNGG 1 cut(s) 747
MunI CAATTG 1 cut(s) 556
Mva1269I GAATGC 1 cut(s) 491
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 35, 83, 854
NdeII GATC 3 cut(s) 406, 529, 1075
NlaIV GGNNCC 2 cut(s) 442, 459
NmuCI GTSAC 1 cut(s) 265
PceI AGGCCT 1 cut(s) 134
PctI GAATGC 1 cut(s) 491
PfeI GAWTC 1 cut(s) 961
PkrI GCNGC 5 cut(s) 28, 613, 640, 859, 913
PleI GAGTC 4 cut(s) 86, 119, 194, 398
PpsI GAGTC 4 cut(s) 86, 119, 194, 398
PshAI GACNNNNGTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 2 cut(s) 442, 459
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 4 cut(s) 36, 440, 441, 839
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 3 cut(s) 576, 666, 816
RsaNI GTAC 3 cut(s) 575, 665, 815
RseI CAYNNNNRTG 2 cut(s) 732, 1068
SaqAI TTAA 4 cut(s) 252, 513, 657, 833
SatI GCNGC 5 cut(s) 27, 612, 639, 858, 912
Sau3AI GATC 3 cut(s) 406, 529, 1075
Sau96I GGNCC 4 cut(s) 36, 440, 441, 839
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 4 cut(s) 86, 120, 194, 399
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 2 cut(s) 423, 444
SfaNI GCATC 2 cut(s) 436, 1033
SinI GGWCC 1 cut(s) 839
SmiMI CAYNNNNRTG 2 cut(s) 732, 1068
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
SspMI CTAG 2 cut(s) 387, 711
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 3 cut(s) 469, 790, 1034
TaqI TCGA 6 cut(s) 105, 129, 198, 623, 947, 1004
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 961
Tru1I TTAA 4 cut(s) 252, 513, 657, 833
Tru9I TTAA 4 cut(s) 252, 513, 657, 833
TscAI CASTG 5 cut(s) 56, 304, 373, 474, 1039
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 4 cut(s) 611, 638, 857, 911
Tsp45I GTSAC 1 cut(s) 265
TspRI CASTG 5 cut(s) 56, 304, 373, 474, 1039
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 1 cut(s) 591
XspI CTAG 2 cut(s) 387, 711
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.