pycom17g23920

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006101
Physical Location & Seq
Forward (+)
22187689 .. 22190430
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
ATGATATCTCACTCAGCTTCGCTCTTCCGAATTTCCAATTCACGTTTCTTCTCATGTCATCTGTCGTTTTGTGTACATAAGAAACACAGCAGAGGCCATGGACTCCGACACCACTCCTCTCCACGACCCTCTCTTGCCCTGGCTCTGGTCCGTGAAGCAAGCCCTAGAAGATTTTCACTCCGGAAAGGATTACGGGTCGGATCGATCACTAAGCTTCTATCCGACTGCATCGCCGCATTCAAGGACAATGCTCAATACAGGGGCGACATCAGATTTCTCAAGATTTGGTTCCTCTATATGGATACCAGTGAAGATTTCGAAAGCATTTTTCTCGAAATGTTGCACAGGGAGATTTGTGTTGGCCACTCCTTGCTTTATGTCTGGTACGCAACCTTTCTCGAAGCCAAAGAGAAATTGTACGATGCACACATGGTTTATCAGATGGGTATTTCAAGGAATGCTAAGCCAGTTGAGTGGGTGGAGAAGGCGCATGCCTTATTTCTTGCTCGGATGTATAGAATAGCTTCTTCAGCTCAGAAGATTGGAAACGATGAAGCAGTTCCTCTTGGGAGCCTCATTAACCCATGGTCCAGCTCCACCATGAAAGACCTTTTGAAGAAAGTTAATCATCAACTTACAAAATATGATGGATACCTTTCTACTACTAAAGCTTACTCGGGAAAAGTGGCCTTGTCTTCTCTCAAGAACTCGTCTAGGAACAAAACTATTGAGATAGGTCGTACGAAGTACCAGATCACTGGTTGTGCAGGCCAGGGTGGCTTTGCTCAAGTATATAAAGCATATGTCAACTGTAATCCTGAAGATGTTGTTGCATTGAAGATACAAAAGCCTGCTTTCCCTTGGGAGTTCTATATGTATCGTCAACTTGATCAACGGATCTCAGACAAGGAAAGGTCAAGCTTTGGGTTTGCTCATAGAATGCATCTCTATCCCGACTGTAGCATTCTCGTCTCCGACTATCTTTCTAGTGGAACACTTCAGGTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

337

Amino Acids

38.38

Weight (kDa)

9.58

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 68 - 150 1.5e-20 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 1 cut(s) 234
AclWI GGATC 2 cut(s) 208, 905
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 3 cut(s) 513, 840, 983
AfaI GTAC 5 cut(s) 75, 386, 419, 742, 749
AfiI CCNNNNNNNGG 2 cut(s) 145, 298
AgsI TTSAA 4 cut(s) 241, 453, 616, 838
AjnI CCWGG 2 cut(s) 138, 771
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 7 cut(s) 17, 214, 524, 533, 594, 671, 921
AluI AGCT 7 cut(s) 17, 214, 524, 533, 594, 671, 921
Alw26I GTCTC 1 cut(s) 976
AlwI GGATC 2 cut(s) 208, 905
Ama87I CYCGRG 1 cut(s) 676
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 4 cut(s) 94, 361, 687, 769
ApoI RAATTY 1 cut(s) 30
Asp700I GAANNNNTTC 3 cut(s) 172, 523, 558
AspLEI GCGC 1 cut(s) 490
AspS9I GGNCC 2 cut(s) 148, 588
AsuII TTCGAA 1 cut(s) 318
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 2 cut(s) 148, 588
BalI TGGCCA 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 687
BccI CCATC 2 cut(s) 436, 641
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 140, 773
BciVI GTATCC 2 cut(s) 295, 644
BclI TGATCA 1 cut(s) 889
BcoDI GTCTC 1 cut(s) 976
BfaI CTAG 3 cut(s) 165, 714, 987
BfmI CTRYAG 1 cut(s) 958
BfuI GTATCC 2 cut(s) 295, 644
BglI GCCNNNNNGGC 1 cut(s) 777
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 2 cut(s) 140, 773
Bme18I GGWCC 2 cut(s) 148, 588
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 2 cut(s) 148, 588
BmiI GGNNCC 2 cut(s) 290, 572
BmrFI CCNGG 2 cut(s) 140, 773
BmsI GCATC 3 cut(s) 237, 412, 952
BpiI GAAGAC 1 cut(s) 687
Bpu1102I GCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 3 cut(s) 263, 686, 771
Bsa29I ATCGAT 1 cut(s) 203
BsaJI CCNNGG 5 cut(s) 97, 138, 584, 772, 860
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 298
Bse1I ACTGG 3 cut(s) 306, 467, 763
BseAI TCCGGA 1 cut(s) 180
BseBI CCWGG 2 cut(s) 140, 773
BseCI ATCGAT 1 cut(s) 203
BseDI CCNNGG 5 cut(s) 97, 138, 584, 772, 860
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 2 cut(s) 145, 298
BseMII CTCAG 3 cut(s) 27, 548, 915
BseNI ACTGG 3 cut(s) 306, 467, 763
BseRI GAGGAG 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 786
BshFI GGCC 4 cut(s) 96, 363, 689, 771
BshVI ATCGAT 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 1 cut(s) 181
BsiWI CGTACG 1 cut(s) 740
BslI CCNNNNNNNGG 2 cut(s) 145, 298
BsmAI GTCTC 1 cut(s) 976
BsmBI CGTCTC 1 cut(s) 976
BsmI GAATGC 4 cut(s) 236, 463, 945, 963
BsnI GGCC 4 cut(s) 96, 363, 689, 771
BsoBI CYCGRG 1 cut(s) 676
Bsp119I TTCGAA 1 cut(s) 318
Bsp13I TCCGGA 1 cut(s) 180
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 5 cut(s) 200, 204, 753, 889, 897
Bsp1720I GCTNAGC 1 cut(s) 462
Bsp19I CCATGG 2 cut(s) 97, 584
BspACI CCGC 1 cut(s) 234
BspANI GGCC 4 cut(s) 96, 363, 689, 771
BspCNI CTCAG 3 cut(s) 26, 547, 914
BspDI ATCGAT 1 cut(s) 203
BspEI TCCGGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 290, 572
BspPI GGATC 2 cut(s) 208, 905
BspQI GCTCTTC 1 cut(s) 29
BspT104I TTCGAA 1 cut(s) 318
BsrGI TGTACA 1 cut(s) 73
BsrI ACTGG 3 cut(s) 306, 467, 763
BssECI CCNNGG 5 cut(s) 97, 138, 584, 772, 860
BssMI GATC 5 cut(s) 200, 204, 753, 889, 897
BssT1I CCWWGG 3 cut(s) 97, 584, 860
Bst2UI CCWGG 2 cut(s) 140, 773
Bst4CI ACNGT 2 cut(s) 812, 959
Bst6I CTCTTC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 73
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 4 cut(s) 160, 492, 769, 852
BstDEI CTNAG 5 cut(s) 13, 210, 462, 534, 901
BstDSI CCRYGG 2 cut(s) 97, 584
BstF5I GGATG 1 cut(s) 516
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 5 cut(s) 203, 207, 756, 892, 900
BstMAI GTCTC 1 cut(s) 976
BstMBI GATC 5 cut(s) 200, 204, 753, 889, 897
BstMWI GCNNNNNNNGC 2 cut(s) 530, 777
BstNI CCWGG 2 cut(s) 140, 773
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 138, 771
BstSFI CTRYAG 1 cut(s) 958
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 1 cut(s) 897
BstXI CCANNNNNNTGG 2 cut(s) 474, 758
BstYI RGATCY 1 cut(s) 897
Bsu15I ATCGAT 1 cut(s) 203
BsuI GTATCC 2 cut(s) 295, 644
BsuRI GGCC 4 cut(s) 96, 363, 689, 771
BsuTUI ATCGAT 1 cut(s) 203
BtgI CCRYGG 2 cut(s) 97, 584
BtgZI GCGATG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 313, 756
Cac8I GCNNGC 4 cut(s) 160, 492, 769, 852
CfoI GCGC 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 148, 588
ClaI ATCGAT 1 cut(s) 203
Csp6I GTAC 5 cut(s) 74, 385, 418, 741, 748
CviAII CATG 6 cut(s) 54, 98, 430, 491, 585, 601
CviQI GTAC 5 cut(s) 74, 385, 418, 741, 748
DdeI CTNAG 5 cut(s) 13, 210, 462, 534, 901
DpnI GATC 5 cut(s) 202, 206, 755, 891, 899
DpnII GATC 5 cut(s) 200, 204, 753, 889, 897
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 97, 584, 860
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 148, 588
Eco57I CTGAAG 3 cut(s) 513, 840, 983
Eco88I CYCGRG 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 138, 771
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 97, 584, 860
EcoT22I ATGCAT 1 cut(s) 945
ErhI CCWWGG 3 cut(s) 97, 584, 860
Esp3I CGTCTC 1 cut(s) 976
FaeI CATG 6 cut(s) 57, 101, 433, 494, 588, 604
FatI CATG 6 cut(s) 53, 97, 429, 490, 584, 600
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 3 cut(s) 165, 714, 987
GlaI GCGC 1 cut(s) 489
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 4 cut(s) 96, 363, 689, 771
HapII CCGG 1 cut(s) 181
HhaI GCGC 1 cut(s) 490
Hin1II CATG 6 cut(s) 57, 101, 433, 494, 588, 604
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HincII GTYRAC 2 cut(s) 808, 884
HindII GTYRAC 2 cut(s) 808, 884
HindIII AAGCTT 3 cut(s) 212, 669, 919
HinfI GANTC 1 cut(s) 102
HpaII CCGG 1 cut(s) 181
Hpy166II GTNNAC 3 cut(s) 74, 808, 884
Hpy188III TCNNGA 8 cut(s) 181, 280, 332, 398, 678, 703, 818, 953
Hpy8I GTNNAC 3 cut(s) 74, 808, 884
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 2 cut(s) 812, 959
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 6 cut(s) 228, 343, 425, 767, 833, 943
HpyF10VI GCNNNNNNNGC 2 cut(s) 530, 777
HpyF3I CTNAG 5 cut(s) 13, 210, 462, 534, 901
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 6 cut(s) 57, 101, 433, 494, 588, 604
HspAI GCGC 1 cut(s) 488
Kpn2I TCCGGA 1 cut(s) 180
Ksp22I TGATCA 1 cut(s) 889
Kzo9I GATC 5 cut(s) 200, 204, 753, 889, 897
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 2 cut(s) 570, 599
LweI GCATC 3 cut(s) 237, 412, 952
MaeI CTAG 3 cut(s) 165, 714, 987
MaeII ACGT 1 cut(s) 43
MalI GATC 5 cut(s) 202, 206, 755, 891, 899
MboI GATC 5 cut(s) 200, 204, 753, 889, 897
MflI RGATCY 1 cut(s) 897
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 3 cut(s) 30, 37, 413
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 4 cut(s) 130, 178, 246, 999
MnlI CCTC 6 cut(s) 86, 127, 139, 302, 573, 584
Mox20I TGGCCA 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 945
MroI TCCGGA 1 cut(s) 180
MroXI GAANNNNTTC 3 cut(s) 172, 523, 558
MscI TGGCCA 1 cut(s) 363
MseI TTAA 3 cut(s) 579, 624, 1009
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 2 cut(s) 140, 773
Mva1269I GAATGC 4 cut(s) 236, 463, 945, 963
MvaI CCWGG 2 cut(s) 140, 773
MwoI GCNNNNNNNGC 2 cut(s) 530, 777
NcoI CCATGG 2 cut(s) 97, 584
NdeI CATATG 1 cut(s) 802
NdeII GATC 5 cut(s) 200, 204, 753, 889, 897
NlaIII CATG 6 cut(s) 57, 101, 433, 494, 588, 604
NlaIV GGNNCC 2 cut(s) 290, 572
NsiI ATGCAT 1 cut(s) 945
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 1 cut(s) 318
PaeI GCATGC 1 cut(s) 494
PciSI GCTCTTC 1 cut(s) 29
PctI GAATGC 4 cut(s) 236, 463, 945, 963
PdmI GAANNNNTTC 3 cut(s) 172, 523, 558
Pfl23II CGTACG 1 cut(s) 740
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 138, 771
PspGI CCWGG 2 cut(s) 138, 771
PspLI CGTACG 1 cut(s) 740
PspN4I GGNNCC 2 cut(s) 290, 572
PspPI GGNCC 2 cut(s) 148, 588
PsuI RGATCY 1 cut(s) 897
RsaI GTAC 5 cut(s) 75, 386, 419, 742, 749
RsaNI GTAC 5 cut(s) 74, 385, 418, 741, 748
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 3 cut(s) 579, 624, 1009
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 5 cut(s) 200, 204, 753, 889, 897
Sau96I GGNCC 2 cut(s) 148, 588
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 140, 773
SfaNI GCATC 3 cut(s) 237, 412, 952
SfcI CTRYAG 1 cut(s) 958
SfuI TTCGAA 1 cut(s) 318
SinI GGWCC 2 cut(s) 148, 588
SmlI CTYRAG 3 cut(s) 278, 701, 786
SmoI CTYRAG 3 cut(s) 278, 701, 786
SphI GCATGC 1 cut(s) 494
Sse9I AATT 3 cut(s) 30, 37, 413
SsiI CCGC 1 cut(s) 234
SspMI CTAG 3 cut(s) 165, 714, 987
StyD4I CCNGG 2 cut(s) 138, 771
StyI CCWWGG 3 cut(s) 97, 584, 860
TaaI ACNGT 2 cut(s) 812, 959
TaiI ACGT 1 cut(s) 46
TaqI TCGA 4 cut(s) 203, 318, 333, 399
TasI AATT 3 cut(s) 30, 37, 413
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 3 cut(s) 579, 624, 1009
Tru9I TTAA 3 cut(s) 579, 624, 1009
TscAI CASTG 2 cut(s) 313, 763
TspDTI ATGAA 2 cut(s) 567, 617
TspGWI ACGGA 2 cut(s) 140, 910
TspRI CASTG 2 cut(s) 313, 763
VpaK11BI GGWCC 2 cut(s) 148, 588
XapI RAATTY 1 cut(s) 30
XceI RCATGY 1 cut(s) 494
XmnI GAANNNNTTC 3 cut(s) 172, 523, 558
XspI CTAG 3 cut(s) 165, 714, 987
Zsp2I ATGCAT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.