pycom17g24610

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009482
Physical Location & Seq
Reverse (-)
22851012 .. 22853466
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGCCGACGGCATCTCCGCAGCCGAATCCGTTTGGGAATACGTTTTATGGTGCTAGTTCAGGACTTATCCGTGGTGGATTGGGTGCATATGGAGAAAAGATTTTAGGATCAAGCTCTGAGTATGTACAAAGCAATATCAGTAGGTATTTCTCTGATCCGCAATACTACTTCCAAGTGAATGACCAGTATGTGAGGAACAAATTGAAGGTCATTCTATTTCCATTTCTCCACAGGGGCCATTGGACCAGAATAACTGAGCCAGTAGGAGGCAGGCTTTCTTATAAGCCCCCAATCTATGACATCAACGCACCAGACCTATACATTCCGTTTATGGCATTTGGTACCTATGTGGTTCTGGCTGGCTTCTCATTGGGTCTTCAGGGAAAGTTTAGCCCTGAAGCTCTAAACTGGTTGTTTATCAAGGGCTTGCTTGGCTGGTTTATGCAAGTTGCACTACTGAAAGTATCATTGCTTTCCTTGGGCGGTGGTGAGGCACCATTGCTTGACATGGTGGCATATGCTGGGTATGCTTTCACGGGGTTGTGCTTGGCTGTACTTGGACGGATTATCTTGAGCTACTCGTACTACTTTTTGATGCCTTGGACGTGTATATGCATGGGAGTCTTCTTGGTGAAGACTATGAAGAGAGTTCTCTTTGCGGAGGTGAGGAGTTATGATTCAAGCAAGCACCATTATCTGTTGATCTTTATTGCTTTGGCTCAGTTCCCCTTGTTTACATGGCTTGGCAACGTTACTGTTAATTGGCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

258

Amino Acids

28.88

Weight (kDa)

9.23

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 29 - 248 3.9e-56 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 341, 493
AciI CCGC 4 cut(s) 17, 158, 483, 659
AclI AACGTT 1 cut(s) 750
AclWI GGATC 2 cut(s) 115, 149
AcuI CTGAAG 2 cut(s) 362, 417
AfaI GTAC 4 cut(s) 126, 343, 555, 584
AfiI CCNNNNNNNGG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 605
AgsI TTSAA 2 cut(s) 205, 681
AjiI CACGTC 1 cut(s) 606
AluBI AGCT 3 cut(s) 114, 401, 576
AluI AGCT 3 cut(s) 114, 401, 576
AlwI GGATC 2 cut(s) 115, 149
AoxI GGCC 1 cut(s) 235
ApeKI GCWGC 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 235, 243
AsuHPI GGTGA 3 cut(s) 500, 643, 676
AvaII GGWCC 1 cut(s) 243
BaeI ACNNNNGTAYC 2 cut(s) 333, 366
BanI GGYRCC 2 cut(s) 341, 493
BbsI GAAGAC 3 cut(s) 368, 616, 641
BbvI GCAGC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 24
BfaI CTAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 20
BlsI GCNGC 1 cut(s) 21
Bme18I GGWCC 1 cut(s) 243
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 2 cut(s) 235, 243
BmiI GGNNCC 3 cut(s) 236, 343, 495
BmsI GCATC 2 cut(s) 20, 585
BpiI GAAGAC 3 cut(s) 368, 616, 641
BpuEI CTTGAG 1 cut(s) 592
BsaJI CCNNGG 3 cut(s) 70, 477, 599
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 184, 260, 413
Bse3DI GCAATG 2 cut(s) 467, 497
BseDI CCNNGG 3 cut(s) 70, 477, 599
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 2 cut(s) 467, 497
BseMII CTCAG 3 cut(s) 108, 246, 734
BseNI ACTGG 3 cut(s) 184, 260, 413
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 1 cut(s) 237
BshNI GGYRCC 2 cut(s) 341, 493
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 3 cut(s) 107, 154, 702
BspACI CCGC 4 cut(s) 17, 158, 483, 659
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 3 cut(s) 109, 247, 733
BspLI GGNNCC 3 cut(s) 236, 343, 495
BspPI GGATC 2 cut(s) 115, 149
BspT107I GGYRCC 2 cut(s) 341, 493
BsrDI GCAATG 2 cut(s) 467, 497
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 3 cut(s) 184, 260, 413
BssECI CCNNGG 3 cut(s) 70, 477, 599
BssMI GATC 3 cut(s) 107, 154, 702
BssT1I CCWWGG 2 cut(s) 477, 599
Bst4CI ACNGT 1 cut(s) 757
Bst6I CTCTTC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 4 cut(s) 272, 361, 428, 686
BstDEI CTNAG 3 cut(s) 117, 255, 720
BstDSI CCRYGG 1 cut(s) 70
BstKTI GATC 3 cut(s) 110, 157, 705
BstMBI GATC 3 cut(s) 107, 154, 702
BstMWI GCNNNNNNNGC 2 cut(s) 432, 527
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 3 cut(s) 368, 616, 641
BsuRI GGCC 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 70
BtrI CACGTC 1 cut(s) 606
Cac8I GCNNGC 4 cut(s) 272, 361, 428, 686
Cfr13I GGNCC 2 cut(s) 235, 243
Csp6I GTAC 4 cut(s) 125, 342, 554, 583
CviAII CATG 3 cut(s) 508, 616, 738
CviQI GTAC 4 cut(s) 125, 342, 554, 583
DdeI CTNAG 3 cut(s) 117, 255, 720
DpnI GATC 3 cut(s) 109, 156, 704
DpnII GATC 3 cut(s) 107, 154, 702
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 477, 599
Eco47I GGWCC 1 cut(s) 243
Eco57I CTGAAG 2 cut(s) 362, 417
EcoT14I CCWWGG 2 cut(s) 477, 599
EcoT22I ATGCAT 1 cut(s) 617
ErhI CCWWGG 2 cut(s) 477, 599
FaeI CATG 3 cut(s) 511, 619, 741
FatI CATG 3 cut(s) 507, 615, 737
FauNDI CATATG 2 cut(s) 88, 517
Fnu4HI GCNGC 1 cut(s) 20
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 20
GsaI CCCAGC 1 cut(s) 525
HaeIII GGCC 1 cut(s) 237
Hin1II CATG 3 cut(s) 511, 619, 741
HinfI GANTC 3 cut(s) 25, 621, 677
HphI GGTGA 3 cut(s) 500, 643, 676
Hpy166II GTNNAC 1 cut(s) 735
Hpy188I TCNGA 2 cut(s) 118, 154
Hpy188III TCNNGA 2 cut(s) 60, 571
Hpy8I GTNNAC 1 cut(s) 735
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 757
HpyCH4IV ACGT 3 cut(s) 41, 605, 750
HpyCH4V TGCA 4 cut(s) 86, 445, 452, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 527
HpyF3I CTNAG 3 cut(s) 117, 255, 720
HpySE526I ACGT 3 cut(s) 41, 605, 750
Hsp92II CATG 3 cut(s) 511, 619, 741
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 107, 154, 702
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 2 cut(s) 20, 585
MaeI CTAG 1 cut(s) 54
MaeII ACGT 3 cut(s) 41, 605, 750
MaeIII GTNAC 1 cut(s) 751
MalI GATC 3 cut(s) 109, 156, 704
MboI GATC 3 cut(s) 107, 154, 702
MboII GAAGA 4 cut(s) 368, 616, 646, 655
MluCI AATT 2 cut(s) 200, 760
MlyI GAGTC 1 cut(s) 630
MnlI CCTC 5 cut(s) 186, 260, 484, 655, 660
Mph1103I ATGCAT 1 cut(s) 617
MseI TTAA 1 cut(s) 759
MwoI GCNNNNNNNGC 2 cut(s) 432, 527
NdeI CATATG 2 cut(s) 88, 517
NdeII GATC 3 cut(s) 107, 154, 702
NlaIII CATG 3 cut(s) 511, 619, 741
NlaIV GGNNCC 3 cut(s) 236, 343, 495
NsiI ATGCAT 1 cut(s) 617
PfeI GAWTC 2 cut(s) 25, 677
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
PsiI TTATAA 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 521
PspN4I GGNNCC 3 cut(s) 236, 343, 495
PspPI GGNCC 2 cut(s) 235, 243
RsaI GTAC 4 cut(s) 126, 343, 555, 584
RsaNI GTAC 4 cut(s) 125, 342, 554, 583
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 3 cut(s) 107, 154, 702
Sau96I GGNCC 2 cut(s) 235, 243
SchI GAGTC 1 cut(s) 630
SfaNI GCATC 2 cut(s) 20, 585
SinI GGWCC 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
Sse9I AATT 2 cut(s) 200, 760
SsiI CCGC 4 cut(s) 17, 158, 483, 659
SspMI CTAG 1 cut(s) 54
StyI CCWWGG 2 cut(s) 477, 599
TaaI ACNGT 1 cut(s) 757
TaiI ACGT 3 cut(s) 44, 608, 753
TasI AATT 2 cut(s) 200, 760
TatI WGTACW 2 cut(s) 124, 553
TfiI GAWTC 2 cut(s) 25, 677
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TseI GCWGC 1 cut(s) 19
TspDTI ATGAA 1 cut(s) 656
TspGWI ACGGA 4 cut(s) 18, 59, 315, 577
VpaK11BI GGWCC 1 cut(s) 243
XspI CTAG 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.