pycom17g26890

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008565
Physical Location & Seq
Forward (+)
25183415 .. 25189220
5806 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGCGGAGGGCCAAAACCACTGTCCTCCTCGTCGCTGCAACTGGCCTCACCTTCCACGCCTTCAACTCCAATTTCCTCCCATCCTCCTCCGCCGATTTCCTCCCATCCTTCCCCGAACAACTCCGCACACCAATTCACGGCGTCGTTCGGTCTTCCCGCGCAATCTCCACCATTGCGTTGACCGCCGTTGACTACAAGCTCACTTTGCGCGGAATCCCAGCGGACTCCGATGAGTACCGTCAGAAGCAATCCCAGGTTCATAGTCGTTCCGCTTCGAGAATTCGGAAGCTGTGTGAGGCCAATAGAGGGTTTTATGTGAAAGCCGGTCAATTTGTGGCCGCTCTGCGGCAGGTTCCGAAAGAGTACTCATCAACCCTTTCCTCATTGCAGGATCAGGCAGTTCCTTGTCATTTCAAAGCCATTAAAGAAGTGCTGATCCGCAATCTGGGACACAAGCTGTCAGAAGTGTTTCTGTCACTTGATGAGCTTCCCATAGCTGCTGCATCTATTGCTCAAGTACACAAGGGAGTTCTTAAGGATCATCAAGAAGTAGCAATTAAGGTGCAGTACCCAGGTCTGGAGCAGCACATGAAAATAGATACGGCAACCATGTATTTCCTTTCAAAATCCCTTACATGGTTTTTCCCGGAATACAGATTTGACTGGCTGGTATCAGAGTTCGTGCATGCTATATCTCTCGAACTTGACTTTATTCAGGAGGCTAGGAATTCTGAGACAACTGCCACTAACTTTGCAAACAACAAATGGGTCAAGATTCCTCGTATATTTTGGGACTTGACCACTACTCAAGTGCTGACAATGGAATTCTGTACAGGACAAAAGGTTGATGATGTACAGTTTCTGAAGGAAAGGAGAATAAACCCAATGAAGGTAGCAGAAGTATTGTTGGAAGCGTTTGCTGAAATGATTTTTATCCATGGTTTCCTGCATGGATCGATCCACACCCTGGTAACATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

327

Amino Acids

37.03

Weight (kDa)

9.01

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 129 - 321 5.7e-65 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AccB7I CCANNNNNTGG 1 cut(s) 967
AccBSI CCGCTC 1 cut(s) 341
AccII CGCG 2 cut(s) 159, 210
AclWI GGATC 5 cut(s) 399, 430, 546, 952, 961
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 3 cut(s) 279, 727, 824
AcuI CTGAAG 1 cut(s) 884
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 6 cut(s) 236, 365, 519, 569, 832, 855
AfiI CCNNNNNNNGG 8 cut(s) 137, 306, 345, 445, 577, 636, 889, 967
AflII CTTAAG 1 cut(s) 533
AgsI TTSAA 3 cut(s) 64, 415, 624
AjnI CCWGG 3 cut(s) 252, 571, 966
AluBI AGCT 5 cut(s) 199, 289, 457, 487, 497
AluI AGCT 5 cut(s) 199, 289, 457, 487, 497
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 5 cut(s) 399, 430, 546, 952, 961
AlwNI CAGNNNCTG 1 cut(s) 862
AoxI GGCC 4 cut(s) 9, 43, 297, 336
ApeKI GCWGC 4 cut(s) 35, 497, 500, 583
ApoI RAATTY 3 cut(s) 279, 727, 824
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 2 cut(s) 161, 210
AspS9I GGNCC 1 cut(s) 9
AsuC2I CCSGG 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 22, 484, 487, 595
BccI CCATC 2 cut(s) 88, 112
BceAI ACGGC 3 cut(s) 154, 170, 618
BciT130I CCWGG 3 cut(s) 254, 573, 968
BcnI CCSGG 1 cut(s) 647
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 1 cut(s) 723
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 6 cut(s) 36, 339, 347, 498, 501, 584
BlsI GCNGC 6 cut(s) 37, 340, 348, 499, 502, 585
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 254, 573, 647, 968
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 4 cut(s) 254, 573, 647, 968
BmsI GCATC 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 599
BpuEI CTTGAG 2 cut(s) 498, 792
BpuMI CCSGG 1 cut(s) 647
Bsa29I ATCGAT 1 cut(s) 956
BsaBI GATNNNNATC 1 cut(s) 932
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 4 cut(s) 252, 571, 937, 966
Bsc4I CCNNNNNNNGG 8 cut(s) 137, 306, 345, 445, 577, 636, 889, 967
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 46, 668
Bse3DI GCAATG 2 cut(s) 171, 383
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 3 cut(s) 254, 573, 968
BseCI ATCGAT 1 cut(s) 956
BseDI CCNNGG 4 cut(s) 252, 571, 937, 966
BseGI GGATG 2 cut(s) 80, 104
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 8 cut(s) 137, 306, 345, 445, 577, 636, 889, 967
BseMI GCAATG 2 cut(s) 171, 383
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 2 cut(s) 46, 668
BseRI GAGGAG 2 cut(s) 17, 76
BseXI GCAGC 4 cut(s) 22, 484, 487, 595
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 2 cut(s) 159, 210
BshFI GGCC 4 cut(s) 11, 45, 299, 338
BshVI ATCGAT 1 cut(s) 956
BsiSI CCGG 2 cut(s) 324, 647
BslFI GGGAC 2 cut(s) 462, 806
BslI CCNNNNNNNGG 8 cut(s) 137, 306, 345, 445, 577, 636, 889, 967
BsmAI GTCTC 1 cut(s) 728
BsmFI GGGAC 2 cut(s) 462, 806
BsnI GGCC 4 cut(s) 11, 45, 299, 338
Bsp1407I TGTACA 2 cut(s) 830, 853
Bsp143I GATC 5 cut(s) 391, 435, 538, 953, 957
Bsp19I CCATGG 1 cut(s) 937
BspANI GGCC 4 cut(s) 11, 45, 299, 338
BspCNI CTCAG 1 cut(s) 724
BspDI ATCGAT 1 cut(s) 956
BspFNI CGCG 2 cut(s) 159, 210
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 5 cut(s) 399, 430, 546, 952, 961
BspTI CTTAAG 1 cut(s) 533
BsrBI CCGCTC 1 cut(s) 341
BsrDI GCAATG 2 cut(s) 171, 383
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 2 cut(s) 830, 853
BsrI ACTGG 2 cut(s) 46, 668
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 252, 571, 937, 966
BssMI GATC 5 cut(s) 391, 435, 538, 953, 957
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 937
Bst2UI CCWGG 3 cut(s) 254, 573, 968
Bst4CI ACNGT 3 cut(s) 22, 239, 858
BstACI GRCGYC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 509
BstAUI TGTACA 2 cut(s) 830, 853
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 1 cut(s) 732
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 2 cut(s) 80, 104
BstFNI CGCG 2 cut(s) 159, 210
BstHHI GCGC 2 cut(s) 161, 210
BstKTI GATC 5 cut(s) 394, 438, 541, 956, 960
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 5 cut(s) 391, 435, 538, 953, 957
BstMWI GCNNNNNNNGC 3 cut(s) 182, 205, 509
BstNI CCWGG 3 cut(s) 254, 573, 968
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 252, 571, 645, 966
BstUI CGCG 2 cut(s) 159, 210
BstV1I GCAGC 4 cut(s) 22, 484, 487, 595
BstV2I GAAGAC 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 956
BsuRI GGCC 4 cut(s) 11, 45, 299, 338
BsuTUI ATCGAT 1 cut(s) 956
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 2 cut(s) 80, 104
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 687
CaiI CAGNNNCTG 1 cut(s) 862
CfoI GCGC 2 cut(s) 161, 210
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 956
CseI GACGC 1 cut(s) 130
Csp6I GTAC 6 cut(s) 235, 364, 518, 568, 831, 854
CspCI CAANNNNNGTGG 2 cut(s) 733, 768
CviAII CATG 6 cut(s) 589, 610, 636, 686, 938, 950
CviQI GTAC 6 cut(s) 235, 364, 518, 568, 831, 854
DdeI CTNAG 1 cut(s) 732
DpnI GATC 5 cut(s) 393, 437, 540, 955, 959
DpnII GATC 5 cut(s) 391, 435, 538, 953, 957
EaeI YGGCCR 1 cut(s) 336
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 884
EcoRI GAATTC 3 cut(s) 279, 727, 824
EcoRII CCWGG 3 cut(s) 252, 571, 966
EcoT14I CCWWGG 1 cut(s) 937
ErhI CCWWGG 1 cut(s) 937
FaeI CATG 6 cut(s) 592, 613, 639, 689, 941, 953
FaqI GGGAC 2 cut(s) 462, 806
FatI CATG 6 cut(s) 588, 609, 635, 685, 937, 949
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 6 cut(s) 36, 339, 347, 498, 501, 584
FokI GGATG 2 cut(s) 67, 91
Fsp4HI GCNGC 6 cut(s) 36, 339, 347, 498, 501, 584
FspBI CTAG 1 cut(s) 723
GlaI GCGC 2 cut(s) 160, 209
GluI GCNGC 6 cut(s) 36, 339, 347, 498, 501, 584
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 1 cut(s) 599
HaeIII GGCC 4 cut(s) 11, 45, 299, 338
HapII CCGG 2 cut(s) 324, 647
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 161, 210
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 6 cut(s) 592, 613, 639, 689, 941, 953
Hin6I GCGC 2 cut(s) 159, 208
HinP1I GCGC 2 cut(s) 159, 208
HincII GTYRAC 2 cut(s) 180, 190
HindII GTYRAC 2 cut(s) 180, 190
HinfI GANTC 3 cut(s) 213, 224, 775
HpaII CCGG 2 cut(s) 324, 647
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 180, 190, 520
Hpy188I TCNGA 8 cut(s) 229, 243, 285, 357, 463, 676, 733, 864
Hpy188III TCNNGA 6 cut(s) 276, 545, 578, 698, 716, 772
Hpy8I GTNNAC 3 cut(s) 180, 190, 520
Hpy99I CGWCG 2 cut(s) 35, 146
HpyAV CCTTC 5 cut(s) 61, 70, 118, 859, 883
HpyCH4III ACNGT 3 cut(s) 22, 239, 858
HpyCH4V TGCA 7 cut(s) 38, 388, 503, 565, 685, 755, 949
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 205, 509
HpyF3I CTNAG 1 cut(s) 732
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 6 cut(s) 592, 613, 639, 689, 941, 953
HspAI GCGC 2 cut(s) 159, 208
Kzo9I GATC 5 cut(s) 391, 435, 538, 953, 957
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 4 cut(s) 22, 484, 487, 595
LweI GCATC 1 cut(s) 512
MaeI CTAG 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 474, 970
MalI GATC 5 cut(s) 393, 437, 540, 955, 959
MbiI CCGCTC 1 cut(s) 341
MboI GATC 5 cut(s) 391, 435, 538, 953, 957
MboII GAAGA 1 cut(s) 144
MluCI AATT 7 cut(s) 70, 132, 279, 329, 555, 727, 824
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 3 cut(s) 423, 534, 558
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 2 cut(s) 324, 647
MspR9I CCNGG 4 cut(s) 254, 573, 647, 968
MvaI CCWGG 3 cut(s) 254, 573, 968
MvnI CGCG 2 cut(s) 159, 210
MwoI GCNNNNNNNGC 3 cut(s) 182, 205, 509
NciI CCSGG 1 cut(s) 647
NcoI CCATGG 1 cut(s) 937
NdeII GATC 5 cut(s) 391, 435, 538, 953, 957
NlaIII CATG 6 cut(s) 592, 613, 639, 689, 941, 953
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 474
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 213, 775
PflMI CCANNNNNTGG 1 cut(s) 967
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 6 cut(s) 37, 340, 348, 499, 502, 585
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 252, 571, 966
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 3 cut(s) 252, 571, 966
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 862
RsaI GTAC 6 cut(s) 236, 365, 519, 569, 832, 855
RsaNI GTAC 6 cut(s) 235, 364, 518, 568, 831, 854
SaqAI TTAA 3 cut(s) 423, 534, 558
SatI GCNGC 6 cut(s) 36, 339, 347, 498, 501, 584
Sau3AI GATC 5 cut(s) 391, 435, 538, 953, 957
Sau96I GGNCC 1 cut(s) 9
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 254, 573, 647, 968
SfaNI GCATC 1 cut(s) 512
SmlI CTYRAG 3 cut(s) 513, 533, 807
SmoI CTYRAG 3 cut(s) 513, 533, 807
SphI GCATGC 1 cut(s) 689
Sse9I AATT 7 cut(s) 70, 132, 279, 329, 555, 727, 824
SspMI CTAG 1 cut(s) 723
StyD4I CCNGG 4 cut(s) 252, 571, 645, 966
StyI CCWWGG 1 cut(s) 937
TaaI ACNGT 3 cut(s) 22, 239, 858
TaqI TCGA 3 cut(s) 275, 699, 956
TaqII GACCGA 1 cut(s) 138
TasI AATT 7 cut(s) 70, 132, 279, 329, 555, 727, 824
TatI WGTACW 4 cut(s) 363, 517, 830, 853
TauI GCSGC 2 cut(s) 341, 349
TfiI GAWTC 2 cut(s) 213, 775
Tru1I TTAA 3 cut(s) 423, 534, 558
Tru9I TTAA 3 cut(s) 423, 534, 558
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 4 cut(s) 35, 497, 500, 583
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 3 cut(s) 248, 605, 902
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 967
Vha464I CTTAAG 1 cut(s) 533
XapI RAATTY 3 cut(s) 279, 727, 824
XceI RCATGY 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 1 cut(s) 723
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.