pycom17g27040

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004012
Physical Location & Seq
Reverse (-)
25286480 .. 25290615
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2535 bp
ATGGCTTCTGCAGCAGCCGTCAAACGCTCCGATTCCTTAGCTGATACCATGCCGGACGCCCTGAGGCAGAGCCGGTACCACATGAAGCGGTGCTTTGCAAAGTACATCGAGCAAGGAAGAAGGATCATGAAGCTTCACCATCTGATGAGTGAAATGGAGACAGTCATAGATGACAAGGATGAAAGAACCCAAGTTTTGGGGGGTGTTCTTGGCTATATATTGTGTTCAACTCAGGAAGCTGTTGTTGTTCCTCCACATGTTGTCTTTTCAATAAGACTAGATCCAGGATATTGGGAATTTGTTAAGGTCAGCTCTGAGGATCTCTCAGTTGAGGCTATCACTGTCCGAGACTTCTTGAAATACAAAGAAGCTTTATATGATGAGAAATGGTCAAATGATGAACATGTATTGGAGGTGGATTTCAGAGCAATTGATTTCTCTACTCCTCACTTGACTCTATCTTCCTCGATCGGAAATGGAATCGATTATGTTACCAAGTTCACCACTTCAAAGCTAGCTGGAAAACTGGATAATGCACAACCCCTTGTGGATTACTTAGTTTCTCTACACCATCATGGGGAACAACTTATTCTAAATGAGACCCTCAACACGGCTTCGAAGCTTCAGGCGGCTCTGATTGTAACAGAAGTGTACCTCTCAGCACTTCCTAAGGACACACCATTCCAAAATTTTGAACTAAGGTTTAAAGAGTGGGGCTTTGAGAAGGGGTGGGGAGACACTGCAGAAAGAACAAAGGAGACAATGAAAATACTTTCAGAAGTACTTCAAGCTCCAGACCCATTGAACATGGATAGGTTTCTCAGTAGGCTTCCCACAATATTCAATGTAGTAATATTCTCTCCCCATGGCTACTTTGGCCAAGCGGATGTTCTTGGCTTACCAGACACGGGCGGGCAGGTAGTTTACATTCTGGATCAAGTGAAAGCTATGGCGGAAGAATTGACCCTGAGAATTAAGCAACAAGGACTTACTGTGAAGCCTCAAATTCTTGTGGTGACAAGACTCATACCAGAAGCTAGGGGAACTAAGTGCAACCAGGAGTTGGAACCAATCAATGGCACCAAGTACTCTAACATCCTTAGGGTGCCATTTAGGACAGAAAAGGGGATCCTACGCCGTTGGGTTTCTCGCTTTGACATCTATCCCTATCTTGAGTTGTTTACACAGGATGCGACTGCCAAAATCCTCTACCTGATGGAAGGAAAGCCGGATCTTATTATTGGAAACTACACAGATGGCAATTTGGTGGCGTCTCTCATGGCTAATAAACTTGGGATAACTCAGGCAACTATTGCTCATGCTTTGGAGAAGACCAAGTACGAACATTCAGACATCAACTGGAAGGAGTTAGATCGATCCAAGTATCACTTCTCATGCCAATTTCTTGCTGACACAATCTCAATGAATGCTACAGATTTTGTCATTGCAAGCACATACCAGGAAATTGCAGGAAGCAAAGACAGACCAGGACAATACGAAAGTCATACTGCATTTACGCTCCCGGGGCTCTGTAGAGTTGTTTCTGGCATCAATGTATTTGACCCCAAATTCAACATCGCTGCTCCTGGGGCGGATCAGTCTGTATATTTCCCCTACTCAGAGAAGCAGAAACGGCTCACTTCATTCTATCCTGCCATTGAAGAACTACTGTTTAGTAAAGAGGATAACAGTGAACATCTTGGATTTCTAGTAGAACGGAAGAAACCTATCATCTTCTCAATGGCAAGGCTGGATATTGTTAAAAACATTACCGGGTTGGTTGAGTGGTACGGGAAGAACAAGAGGCTGAGAAATTTGGTTAACCTTGTGGTAGTTGGTGGATTCTTTGACCCTTCGAAATCAAAAGATAGAGAAGAAATCGCTGAAATAAAGAAGATGCATACACTGATAGAGAAGTACCAACTCAGGGGTCAGATCAGATGGATAGCAGCACAGGCTGATAGGAACCGAAATGGAGAGCTCTACCGTTGCATTGCTGACACAAGGGGAGCTTTTGTGCAACCTGCCCTGTATGAAGCATTCGGTCTGACTGTCATTGAGGCAATGAACTGTGGATTGCCCACCTTTGCAACCAACCAAGGAGGCCCGGCTGAAATCATCGTTGATGAGGTCTCTGGTTTCCATATCGATCCTAACAACGGAGATGAAGCAAGCAACAAAATCGCTGATTTCTTTGAGAAGTCCAAGACTGATGCTGCATATTGGGACAGATTTTCCAAAGCAGGCTTGCAGCGCATATACGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGGAGCACTTATACTTTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAGGAACTTGGTGAAGAATGTGCCTGTCCCAAGTGATGAACCTGAGCAACCAGCTGCGCCAAAGCCAACTGCCAAACCACAGACCACATTAAGCTCAAGGCGCTCACAGTCTCGATTGCAAAGGTTGTTTGGAAATTAA

Protein Analysis

845

Amino Acids

96.05

Weight (kDa)

7.59

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 7.5e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 246 7.6e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 269 - 558 1.7e-166 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 573 - 739 1.9e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 576 - 731 1.7e-12 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 907, 2032
Acc65I GGTACC 1 cut(s) 75
AccB1I GGYRCC 3 cut(s) 75, 1079, 1105
AccB7I CCANNNNNTGG 3 cut(s) 196, 1063, 1076
AciI CCGC 6 cut(s) 88, 629, 884, 912, 953, 1592
AcoI YGGCCR 1 cut(s) 877
AcsI RAATTY 5 cut(s) 296, 688, 1005, 1568, 1813
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 2 cut(s) 57, 1271
AfaI GTAC 8 cut(s) 77, 104, 653, 783, 1088, 1340, 1790, 1919
AfiI CCNNNNNNNGG 8 cut(s) 196, 577, 610, 908, 1063, 1076, 1927, 2159
AflIII ACRYGT 2 cut(s) 256, 403
AjnI CCWGG 5 cut(s) 283, 1056, 1458, 1486, 1585
AjuI GAANNNNNNNTTGG 4 cut(s) 873, 905, 1373, 1405
Alw21I GWGCWC 2 cut(s) 1983, 2315
Alw26I GTCTC 8 cut(s) 152, 342, 593, 729, 752, 1278, 2137, 2511
Ama87I CYCGRG 1 cut(s) 1522
AoxI GGCC 2 cut(s) 877, 2104
ApeKI GCWGC 7 cut(s) 11, 14, 1580, 1949, 2216, 2251, 2450
ApoI RAATTY 5 cut(s) 296, 688, 1005, 1568, 1813
Asp700I GAANNNNTTC 1 cut(s) 783
Asp718I GGTACC 1 cut(s) 75
AspLEI GCGC 3 cut(s) 2256, 2455, 2499
AspS9I GGNCC 1 cut(s) 2105
AsuC2I CCSGG 4 cut(s) 1523, 1524, 1774, 2108
AsuHPI GGTGA 4 cut(s) 128, 493, 1027, 2419
AsuII TTCGAA 2 cut(s) 617, 1856
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 1522
AxyI CCTNAGG 3 cut(s) 62, 669, 1100
BalI TGGCCA 1 cut(s) 879
BamHI GGATCC 1 cut(s) 1128
BanI GGYRCC 3 cut(s) 75, 1079, 1105
BanII GRGCYC 2 cut(s) 1530, 1983
BarI GAAGNNNNNNTAC 2 cut(s) 1322, 1354
BbsI GAAGAC 1 cut(s) 1337
Bbv12I GWGCWC 2 cut(s) 1983, 2315
BbvI GCAGC 7 cut(s) 23, 26, 1567, 1961, 2203, 2263, 2437
BccI CCATC 5 cut(s) 147, 579, 1210, 1250, 1935
BceAI ACGGC 4 cut(s) 2, 627, 1122, 1649
BcgI CGANNNNNNTGC 2 cut(s) 2164, 2198
BciT130I CCWGG 5 cut(s) 285, 1058, 1460, 1488, 1587
BcnI CCSGG 4 cut(s) 1523, 1524, 1774, 2108
BcoDI GTCTC 8 cut(s) 152, 342, 593, 729, 752, 1278, 2137, 2511
BfaI CTAG 4 cut(s) 278, 515, 1038, 1709
BfmI CTRYAG 4 cut(s) 9, 741, 1431, 1531
BfoI RGCGCY 1 cut(s) 2500
BfuAI ACCTGC 2 cut(s) 907, 2032
BisI GCNGC 8 cut(s) 12, 15, 630, 1581, 1950, 2217, 2252, 2451
BlsI GCNGC 8 cut(s) 13, 16, 631, 1582, 1951, 2218, 2253, 2452
BmcAI AGTACT 2 cut(s) 783, 1088
Bme1390I CCNGG 9 cut(s) 285, 1058, 1460, 1488, 1523, 1524, 1587, 1774, 2108
BmeT110I CYCGRG 1 cut(s) 1522
BmgT120I GGNCC 1 cut(s) 2105
BmiI GGNNCC 6 cut(s) 77, 1068, 1081, 1107, 1130, 1967
BmrFI CCNGG 9 cut(s) 285, 1058, 1460, 1488, 1523, 1524, 1587, 1774, 2108
BmsI GCATC 4 cut(s) 1180, 1557, 1887, 2203
BmtI GCTAGC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 1337
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 2 cut(s) 777, 2347
Bpu10I CCTNAGC 2 cut(s) 37, 2439
Bpu14I TTCGAA 2 cut(s) 617, 1856
BpuEI CTTGAG 2 cut(s) 1193, 2476
BpuMI CCSGG 4 cut(s) 1523, 1524, 1774, 2108
Bsa29I ATCGAT 3 cut(s) 483, 1375, 2148
BsaHI GRCGYC 2 cut(s) 57, 1271
BsaI GGTCTC 2 cut(s) 593, 2137
BsaJI CCNNGG 5 cut(s) 865, 1522, 1523, 1586, 2098
BsaXI ACNNNNNCTCC 2 cut(s) 2001, 2031
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 577, 610, 908, 1063, 1076, 1927, 2159
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 2 cut(s) 531, 1364
Bse21I CCTNAGG 3 cut(s) 62, 669, 1100
Bse3DI GCAATG 3 cut(s) 1443, 1992, 2070
BseBI CCWGG 5 cut(s) 285, 1058, 1460, 1488, 1587
BseCI ATCGAT 3 cut(s) 483, 1375, 2148
BseDI CCNNGG 5 cut(s) 865, 1522, 1523, 1586, 2098
BseGI GGATG 5 cut(s) 184, 892, 1095, 1195, 2370
BseLI CCNNNNNNNGG 8 cut(s) 196, 577, 610, 908, 1063, 1076, 1927, 2159
BseMI GCAATG 3 cut(s) 1443, 1992, 2070
BseNI ACTGG 2 cut(s) 531, 1364
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 7 cut(s) 23, 26, 1567, 1961, 2203, 2263, 2437
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 879, 2106
BshNI GGYRCC 3 cut(s) 75, 1079, 1105
BshVI ATCGAT 3 cut(s) 483, 1375, 2148
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 1983, 2315
BsiHKCI CYCGRG 1 cut(s) 1522
BsiSI CCGG 6 cut(s) 53, 73, 1229, 1523, 1773, 2108
BslFI GGGAC 2 cut(s) 2240, 2408
BslI CCNNNNNNNGG 8 cut(s) 196, 577, 610, 908, 1063, 1076, 1927, 2159
BsmAI GTCTC 8 cut(s) 152, 342, 593, 729, 752, 1278, 2137, 2511
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 2 cut(s) 2240, 2408
BsmI GAATGC 3 cut(s) 1432, 2039, 2270
BsnI GGCC 2 cut(s) 879, 2106
Bso31I GGTCTC 2 cut(s) 593, 2137
BsoBI CYCGRG 1 cut(s) 1522
Bsp119I TTCGAA 2 cut(s) 617, 1856
Bsp1286I GDGCHC 3 cut(s) 1530, 1983, 2315
Bsp19I CCATGG 1 cut(s) 865
BspACI CCGC 6 cut(s) 88, 629, 884, 912, 953, 1592
BspANI GGCC 2 cut(s) 879, 2106
BspDI ATCGAT 3 cut(s) 483, 1375, 2148
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 6 cut(s) 77, 1068, 1081, 1107, 1130, 1967
BspMAI CTGCAG 2 cut(s) 13, 745
BspMI ACCTGC 2 cut(s) 907, 2032
BspOI GCTAGC 1 cut(s) 518
BspT104I TTCGAA 2 cut(s) 617, 1856
BspT107I GGYRCC 3 cut(s) 75, 1079, 1105
BspTNI GGTCTC 2 cut(s) 593, 2137
BsrDI GCAATG 3 cut(s) 1443, 1992, 2070
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 2 cut(s) 531, 1364
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 5 cut(s) 865, 1522, 1523, 1586, 2098
BssNI GRCGYC 2 cut(s) 57, 1271
BssT1I CCWWGG 2 cut(s) 865, 2098
Bst2UI CCWGG 5 cut(s) 285, 1058, 1460, 1488, 1587
Bst4CI ACNGT 9 cut(s) 163, 343, 994, 1671, 1691, 1988, 2053, 2072, 2505
BstACI GRCGYC 2 cut(s) 57, 1271
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBI TTCGAA 2 cut(s) 617, 1856
BstC8I GCNNGC 6 cut(s) 516, 914, 1450, 2173, 2245, 2249
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 5 cut(s) 184, 892, 1095, 1195, 2370
BstH2I RGCGCY 1 cut(s) 2500
BstHHI GCGC 3 cut(s) 2256, 2455, 2499
BstMAI GTCTC 8 cut(s) 152, 342, 593, 729, 752, 1278, 2137, 2511
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 9 cut(s) 11, 876, 1313, 1525, 1589, 1633, 1955, 2253, 2496
BstNI CCWGG 5 cut(s) 285, 1058, 1460, 1488, 1587
BstNSI RCATGY 2 cut(s) 260, 407
BstSCI CCNGG 9 cut(s) 283, 1056, 1458, 1486, 1521, 1522, 1585, 1772, 2106
BstSFI CTRYAG 4 cut(s) 9, 741, 1431, 1531
BstV1I GCAGC 7 cut(s) 23, 26, 1567, 1961, 2203, 2263, 2437
BstV2I GAAGAC 1 cut(s) 1337
BstX2I RGATCY 4 cut(s) 280, 319, 1128, 1231
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 4 cut(s) 280, 319, 1128, 1231
Bsu15I ATCGAT 3 cut(s) 483, 1375, 2148
Bsu36I CCTNAGG 3 cut(s) 62, 669, 1100
BsuRI GGCC 2 cut(s) 879, 2106
BsuTUI ATCGAT 3 cut(s) 483, 1375, 2148
BtgI CCRYGG 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 1561
BtsCI GGATG 5 cut(s) 184, 892, 1095, 1195, 2370
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 4 cut(s) 339, 738, 1696, 1904
BveI ACCTGC 2 cut(s) 907, 2032
Cac8I GCNNGC 6 cut(s) 516, 914, 1450, 2173, 2245, 2249
CciI TCATGA 1 cut(s) 126
CfoI GCGC 3 cut(s) 2256, 2455, 2499
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 2105
Cfr9I CCCGGG 1 cut(s) 1522
ClaI ATCGAT 3 cut(s) 483, 1375, 2148
CseI GACGC 2 cut(s) 65, 1260
Csp6I GTAC 8 cut(s) 76, 103, 652, 782, 1087, 1339, 1789, 1918
CviQI GTAC 8 cut(s) 76, 103, 652, 782, 1087, 1339, 1789, 1918
DraI TTTAAA 1 cut(s) 706
EaeI YGGCCR 1 cut(s) 877
EciI GGCGGA 2 cut(s) 968, 1607
Ecl136II GAGCTC 1 cut(s) 1981
Eco130I CCWWGG 2 cut(s) 865, 2098
Eco24I GRGCYC 2 cut(s) 1530, 1983
Eco31I GGTCTC 2 cut(s) 593, 2137
Eco53kI GAGCTC 1 cut(s) 1981
Eco57I CTGAAG 1 cut(s) 608
Eco81I CCTNAGG 3 cut(s) 62, 669, 1100
Eco88I CYCGRG 1 cut(s) 1522
EcoICRI GAGCTC 1 cut(s) 1981
EcoRII CCWGG 5 cut(s) 283, 1056, 1458, 1486, 1585
EcoT14I CCWWGG 2 cut(s) 865, 2098
EcoT22I ATGCAT 1 cut(s) 1902
EcoT38I GRGCYC 2 cut(s) 1530, 1983
ErhI CCWWGG 2 cut(s) 865, 2098
Esp3I CGTCTC 1 cut(s) 1278
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 1373, 1405, 1996, 2028
FaqI GGGAC 2 cut(s) 2240, 2408
FauI CCCGC 1 cut(s) 905
Fnu4HI GCNGC 8 cut(s) 12, 15, 630, 1581, 1950, 2217, 2252, 2451
FokI GGATG 5 cut(s) 191, 899, 1082, 1202, 2357
FriOI GRGCYC 2 cut(s) 1530, 1983
Fsp4HI GCNGC 8 cut(s) 12, 15, 630, 1581, 1950, 2217, 2252, 2451
FspBI CTAG 4 cut(s) 278, 515, 1038, 1709
GlaI GCGC 3 cut(s) 2255, 2454, 2498
GluI GCNGC 8 cut(s) 12, 15, 630, 1581, 1950, 2217, 2252, 2451
GsuI CTGGAG 2 cut(s) 777, 2347
HaeII RGCGCY 1 cut(s) 2500
HaeIII GGCC 2 cut(s) 879, 2106
HapII CCGG 6 cut(s) 53, 73, 1229, 1523, 1773, 2108
HgaI GACGC 2 cut(s) 65, 1260
HhaI GCGC 3 cut(s) 2256, 2455, 2499
Hin1I GRCGYC 2 cut(s) 57, 1271
Hin6I GCGC 3 cut(s) 2254, 2453, 2497
HinP1I GCGC 3 cut(s) 2254, 2453, 2497
HincII GTYRAC 1 cut(s) 1822
HindII GTYRAC 1 cut(s) 1822
HindIII AAGCTT 3 cut(s) 131, 369, 620
HinfI GANTC 5 cut(s) 32, 454, 480, 1023, 1842
HpaI GTTAAC 1 cut(s) 1822
HpaII CCGG 6 cut(s) 53, 73, 1229, 1523, 1773, 2108
HphI GGTGA 4 cut(s) 128, 493, 1027, 2419
Hpy166II GTNNAC 6 cut(s) 501, 652, 925, 1182, 1694, 1822
Hpy188III TCNNGA 9 cut(s) 127, 233, 355, 794, 932, 1172, 2326, 2374, 2508
Hpy8I GTNNAC 6 cut(s) 501, 652, 925, 1182, 1694, 1822
HpyAV CCTTC 5 cut(s) 114, 718, 1214, 1357, 1863
HpyCH4III ACNGT 9 cut(s) 163, 343, 994, 1671, 1691, 1988, 2053, 2072, 2505
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 876, 1313, 1525, 1589, 1633, 1955, 2253, 2496
Hsp92I GRCGYC 2 cut(s) 57, 1271
HspAI GCGC 3 cut(s) 2254, 2453, 2497
KpnI GGTACC 1 cut(s) 79
KspAI GTTAAC 1 cut(s) 1822
LmnI GCTCC 6 cut(s) 32, 796, 1524, 1588, 2009, 2310
Lsp1109I GCAGC 7 cut(s) 23, 26, 1567, 1961, 2203, 2263, 2437
LweI GCATC 4 cut(s) 1180, 1557, 1887, 2203
MaeI CTAG 4 cut(s) 278, 515, 1038, 1709
MaeIII GTNAC 3 cut(s) 490, 640, 1015
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 4 cut(s) 280, 319, 1128, 1231
MhlI GDGCHC 3 cut(s) 1530, 1983, 2315
MlsI TGGCCA 1 cut(s) 879
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 2 cut(s) 448, 1017
MmeI TCCRAC 1 cut(s) 1044
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 1902
MroXI GAANNNNTTC 1 cut(s) 783
MscI TGGCCA 1 cut(s) 879
MseI TTAA 8 cut(s) 303, 705, 975, 1761, 1821, 2385, 2486, 2533
MslI CAYNNNNRTG 2 cut(s) 573, 2375
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 2450
MspI CCGG 6 cut(s) 53, 73, 1229, 1523, 1773, 2108
MspR9I CCNGG 9 cut(s) 285, 1058, 1460, 1488, 1523, 1524, 1587, 1774, 2108
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 3 cut(s) 1432, 2039, 2270
MvaI CCWGG 5 cut(s) 285, 1058, 1460, 1488, 1587
MwoI GCNNNNNNNGC 9 cut(s) 11, 876, 1313, 1525, 1589, 1633, 1955, 2253, 2496
NciI CCSGG 4 cut(s) 1523, 1524, 1774, 2108
NcoI CCATGG 1 cut(s) 865
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 6 cut(s) 77, 1068, 1081, 1107, 1130, 1967
NmuCI GTSAC 1 cut(s) 1015
NsiI ATGCAT 1 cut(s) 1902
NspI RCATGY 2 cut(s) 260, 407
NspV TTCGAA 2 cut(s) 617, 1856
PagI TCATGA 1 cut(s) 126
PciI ACATGT 2 cut(s) 256, 403
PctI GAATGC 3 cut(s) 1432, 2039, 2270
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 3 cut(s) 32, 480, 1842
PflMI CCANNNNNTGG 3 cut(s) 196, 1063, 1076
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 8 cut(s) 13, 16, 631, 1582, 1951, 2218, 2253, 2452
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 2 cut(s) 448, 1017
PpsI GAGTC 2 cut(s) 448, 1017
PscI ACATGT 2 cut(s) 256, 403
Psp124BI GAGCTC 1 cut(s) 1983
Psp6I CCWGG 5 cut(s) 283, 1056, 1458, 1486, 1585
PspGI CCWGG 5 cut(s) 283, 1056, 1458, 1486, 1585
PspN4I GGNNCC 6 cut(s) 77, 1068, 1081, 1107, 1130, 1967
PspPI GGNCC 1 cut(s) 2105
PstI CTGCAG 2 cut(s) 13, 745
PsuI RGATCY 4 cut(s) 280, 319, 1128, 1231
PvuI CGATCG 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 2450
RsaI GTAC 8 cut(s) 77, 104, 653, 783, 1088, 1340, 1790, 1919
RsaNI GTAC 8 cut(s) 76, 103, 652, 782, 1087, 1339, 1789, 1918
RseI CAYNNNNRTG 2 cut(s) 573, 2375
SacI GAGCTC 1 cut(s) 1983
SaqAI TTAA 8 cut(s) 303, 705, 975, 1761, 1821, 2385, 2486, 2533
SatI GCNGC 8 cut(s) 12, 15, 630, 1581, 1950, 2217, 2252, 2451
Sau96I GGNCC 1 cut(s) 2105
ScaI AGTACT 2 cut(s) 783, 1088
SchI GAGTC 2 cut(s) 448, 1017
ScrFI CCNGG 9 cut(s) 285, 1058, 1460, 1488, 1523, 1524, 1587, 1774, 2108
SduI GDGCHC 3 cut(s) 1530, 1983, 2315
SfaNI GCATC 4 cut(s) 1180, 1557, 1887, 2203
SfcI CTRYAG 4 cut(s) 9, 741, 1431, 1531
SfuI TTCGAA 2 cut(s) 617, 1856
SmaI CCCGGG 1 cut(s) 1524
SmiMI CAYNNNNRTG 2 cut(s) 573, 2375
SmlI CTYRAG 2 cut(s) 1172, 2491
SmoI CTYRAG 2 cut(s) 1172, 2491
SsiI CCGC 6 cut(s) 88, 629, 884, 912, 953, 1592
SspI AATATT 2 cut(s) 840, 855
SspMI CTAG 4 cut(s) 278, 515, 1038, 1709
SstI GAGCTC 1 cut(s) 1983
StyD4I CCNGG 9 cut(s) 283, 1056, 1458, 1486, 1521, 1522, 1585, 1772, 2106
StyI CCWWGG 2 cut(s) 865, 2098
TaaI ACNGT 9 cut(s) 163, 343, 994, 1671, 1691, 1988, 2053, 2072, 2505
TaqI TCGA 8 cut(s) 108, 467, 483, 617, 1375, 1856, 2148, 2509
TaqII GACCGA 1 cut(s) 2033
TatI WGTACW 3 cut(s) 102, 781, 1086
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 3 cut(s) 32, 480, 1842
Tru1I TTAA 8 cut(s) 303, 705, 975, 1761, 1821, 2385, 2486, 2533
Tru9I TTAA 8 cut(s) 303, 705, 975, 1761, 1821, 2385, 2486, 2533
TscAI CASTG 4 cut(s) 346, 745, 1696, 1911
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 7 cut(s) 11, 14, 1580, 1949, 2216, 2251, 2450
Tsp45I GTSAC 1 cut(s) 1015
TspGWI ACGGA 2 cut(s) 1732, 2175
TspMI CCCGGG 1 cut(s) 1522
TspRI CASTG 4 cut(s) 346, 745, 1696, 1911
Van91I CCANNNNNTGG 3 cut(s) 196, 1063, 1076
XapI RAATTY 5 cut(s) 296, 688, 1005, 1568, 1813
XceI RCATGY 2 cut(s) 260, 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmaI CCCGGG 1 cut(s) 1522
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 4 cut(s) 278, 515, 1038, 1709
ZrmI AGTACT 2 cut(s) 783, 1088
Zsp2I ATGCAT 1 cut(s) 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.