pycom2132g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002132
OG0000295
Physical Location & Seq
Forward (+)
1 .. 845
845 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2132g00010.1

Sequence Viewer

Length: 548 bp
AGGTGAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGAGTATGGTCGCAAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCGCGGCCAGCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTCCACTGAACCTTATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.28

Weight (kDa)

11.27

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000295)

Species Orthologous Gene IDs
pyrus_communis pycom2132g00010
rosa_roxburghii Rroxscaffold_101G00451080 Rroxscaffold_103G00450520 Rroxscaffold_105G00447130 Rroxscaffold_105G00447170 Rroxscaffold_107G00442620 Rroxscaffold_107G00442680 Rroxscaffold_107G00442720 Rroxscaffold_109G00451490 Rroxscaffold_111G00451540 Rroxscaffold_117G00451730 Rroxscaffold_119G00451820 Rroxscaffold_124G00451940 Rroxscaffold_129G00452080 Rroxscaffold_130G00452090 Rroxscaffold_133G00452140 Rroxscaffold_137G00452170 Rroxscaffold_139G00452220 Rroxscaffold_13G00448650 Rroxscaffold_13G00448660 Rroxscaffold_144G00452230 Rroxscaffold_147G00452260 Rroxscaffold_15G00447540 Rroxscaffold_15G00447550 Rroxscaffold_15G00447560 Rroxscaffold_16G00446230 Rroxscaffold_17G00435400 Rroxscaffold_17G00435490 Rroxscaffold_17G00435550 Rroxscaffold_17G00435630 Rroxscaffold_17G00435670 Rroxscaffold_17G00435740 Rroxscaffold_17G00435750 Rroxscaffold_17G00435820 Rroxscaffold_17G00435830 Rroxscaffold_17G00435890 Rroxscaffold_17G00435900 Rroxscaffold_1G00000120 Rroxscaffold_1G00000140 Rroxscaffold_1G00000170 Rroxscaffold_20G00445080 Rroxscaffold_21G00439530 Rroxscaffold_21G00439610 Rroxscaffold_22G00439860 Rroxscaffold_22G00439960 Rroxscaffold_24G00445010 Rroxscaffold_26G00447780 Rroxscaffold_27G00446620 Rroxscaffold_27G00446630 Rroxscaffold_28G00446940 Rroxscaffold_28G00446950 Rroxscaffold_28G00446960 Rroxscaffold_29G00441540 Rroxscaffold_29G00441550 Rroxscaffold_32G00442730 Rroxscaffold_32G00442850 Rroxscaffold_33G00439730 Rroxscaffold_36G00440150 Rroxscaffold_37G00445150 Rroxscaffold_38G00444610 Rroxscaffold_38G00444640 Rroxscaffold_39G00448190 Rroxscaffold_39G00448240 Rroxscaffold_39G00448250 Rroxscaffold_40G00447690 Rroxscaffold_42G00450450 Rroxscaffold_44G00440620 Rroxscaffold_44G00440650 Rroxscaffold_45G00438830 Rroxscaffold_45G00438970 Rroxscaffold_46G00448710 Rroxscaffold_46G00448750 Rroxscaffold_47G00444030 Rroxscaffold_51G00447880 Rroxscaffold_52G00439410 Rroxscaffold_55G00450110 Rroxscaffold_56G00450960 Rroxscaffold_57G00443090 Rroxscaffold_57G00443100 Rroxscaffold_58G00448990 Rroxscaffold_58G00449030 Rroxscaffold_58G00449040 Rroxscaffold_59G00442100 Rroxscaffold_59G00442110 Rroxscaffold_59G00442120 Rroxscaffold_59G00442130 Rroxscaffold_60G00448420 Rroxscaffold_61G00449960 Rroxscaffold_61G00449980 Rroxscaffold_62G00437950 Rroxscaffold_65G00445320 Rroxscaffold_65G00445330 Rroxscaffold_67G00448110 Rroxscaffold_67G00448180 Rroxscaffold_6G00387750 Rroxscaffold_70G00446410 Rroxscaffold_73G00439300 Rroxscaffold_74G00442880 Rroxscaffold_74G00442940 Rroxscaffold_75G00447420 Rroxscaffold_76G00448560 Rroxscaffold_77G00449170 Rroxscaffold_77G00449180 Rroxscaffold_77G00449190 Rroxscaffold_77G00449200 Rroxscaffold_81G00450460 Rroxscaffold_81G00450470 Rroxscaffold_82G00450790 Rroxscaffold_82G00450800 Rroxscaffold_84G00451000 Rroxscaffold_84G00451010 Rroxscaffold_90G00448800 Rroxscaffold_91G00442990 Rroxscaffold_91G00443020 Rroxscaffold_91G00443030 Rroxscaffold_91G00443050 Rroxscaffold_92G00446530 Rroxscaffold_93G00440920 Rroxscaffold_95G00449220 Rroxscaffold_96G00449120 Rroxscaffold_96G00449130 Rroxscaffold_96G00449140 Rroxscaffold_98G00451330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
AciI CCGC 9 cut(s) 94, 115, 283, 298, 325, 438, 486, 494, 510
AclWI GGATC 2 cut(s) 96, 489
AcoI YGGCCR 3 cut(s) 205, 299, 322
AcsI RAATTY 2 cut(s) 6, 377
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 432
AgsI TTSAA 2 cut(s) 370, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 4 cut(s) 174, 278, 305, 403
AluI AGCT 4 cut(s) 174, 278, 305, 403
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 2 cut(s) 96, 489
AoxI GGCC 4 cut(s) 205, 299, 322, 331
ApeKI GCWGC 1 cut(s) 505
ApoI RAATTY 2 cut(s) 6, 377
AseI ATTAAT 1 cut(s) 65
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 464
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 15, 481
AvaII GGWCC 1 cut(s) 464
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 176
BbvCI CCTCAGC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 492
BceAI ACGGC 1 cut(s) 192
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 251
BfaI CTAG 2 cut(s) 275, 383
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 4 cut(s) 299, 325, 487, 506
BlsI GCNGC 4 cut(s) 300, 326, 488, 507
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 122, 150
BmrFI CCNGG 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 358
Bpu10I CCTNAGC 2 cut(s) 262, 328
BpuMI CCSGG 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 50, 152, 334
BsaWI WCCGGW 1 cut(s) 466
Bse118I RCCGGY 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 350
BseDI CCNNGG 3 cut(s) 50, 152, 334
BseGI GGATG 3 cut(s) 60, 102, 290
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 492
Bsh1236I CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
BshFI GGCC 4 cut(s) 207, 301, 324, 333
BshNI GGYRCC 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 3 cut(s) 118, 152, 467
BslFI GGGAC 2 cut(s) 329, 403
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 2 cut(s) 329, 403
BsnI GGCC 4 cut(s) 207, 301, 324, 333
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 88, 347, 481
Bsp68I TCGCGA 1 cut(s) 520
BspACI CCGC 9 cut(s) 94, 115, 283, 298, 325, 438, 486, 494, 510
BspANI GGCC 4 cut(s) 207, 301, 324, 333
BspCNI CTCAG 1 cut(s) 275
BspFNI CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
BspLI GGNNCC 2 cut(s) 122, 150
BspPI GGATC 2 cut(s) 96, 489
BspT107I GGYRCC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 50, 152, 334
BssMI GATC 3 cut(s) 88, 347, 481
BssNI GRCGYC 1 cut(s) 515
BssT1I CCWWGG 2 cut(s) 50, 334
BstACI GRCGYC 1 cut(s) 515
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 5 cut(s) 119, 268, 303, 405, 510
BstDEI CTNAG 4 cut(s) 213, 262, 309, 328
BstF5I GGATG 3 cut(s) 60, 102, 290
BstFNI CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 3 cut(s) 91, 350, 484
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 3 cut(s) 88, 347, 481
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
BstV1I GCAGC 1 cut(s) 492
BstV2I GAAGAC 1 cut(s) 358
BsuRI GGCC 4 cut(s) 207, 301, 324, 333
BtsCI GGATG 3 cut(s) 60, 102, 290
BtsIMutI CAGTG 1 cut(s) 529
BtuMI TCGCGA 1 cut(s) 520
Cac8I GCNNGC 5 cut(s) 119, 268, 303, 405, 510
CfoI GCGC 1 cut(s) 392
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 431
CviAII CATG 1 cut(s) 203
CviJI RGCY 9 cut(s) 174, 207, 266, 278, 301, 305, 324, 333, 403
CviKI_1 RGCY 9 cut(s) 174, 207, 266, 278, 301, 305, 324, 333, 403
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 4 cut(s) 213, 262, 309, 328
DpnI GATC 3 cut(s) 90, 349, 483
DpnII GATC 3 cut(s) 88, 347, 481
EaeI YGGCCR 3 cut(s) 205, 299, 322
EciI GGCGGA 2 cut(s) 104, 109
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 50, 334
Eco24I GRGCYC 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 464
Eco53kI GAGCTC 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 174
EcoRI GAATTC 2 cut(s) 6, 377
EcoT14I CCWWGG 2 cut(s) 50, 334
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 2 cut(s) 50, 334
FaeI CATG 1 cut(s) 206
FaiI YATR 7 cut(s) 20, 128, 162, 190, 204, 281, 321
FaqI GGGAC 2 cut(s) 329, 403
FatI CATG 1 cut(s) 202
FauI CCCGC 2 cut(s) 487, 517
Fnu4HI GCNGC 4 cut(s) 299, 325, 487, 506
FokI GGATG 3 cut(s) 67, 109, 277
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 4 cut(s) 299, 325, 487, 506
FspBI CTAG 2 cut(s) 275, 383
GlaI GCGC 1 cut(s) 391
GluI GCNGC 4 cut(s) 299, 325, 487, 506
HaeIII GGCC 4 cut(s) 207, 301, 324, 333
HapII CCGG 3 cut(s) 118, 152, 467
HgaI GACGC 1 cut(s) 500
HhaI GCGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HincII GTYRAC 2 cut(s) 249, 411
HindII GTYRAC 2 cut(s) 249, 411
HinfI GANTC 3 cut(s) 111, 184, 394
HpaI GTTAAC 1 cut(s) 249
HpaII CCGG 3 cut(s) 118, 152, 467
HphI GGTGA 2 cut(s) 15, 481
Hpy166II GTNNAC 4 cut(s) 249, 411, 433, 529
Hpy188I TCNGA 1 cut(s) 481
Hpy188III TCNNGA 3 cut(s) 70, 181, 519
Hpy8I GTNNAC 4 cut(s) 249, 411, 433, 529
Hpy99I CGWCG 4 cut(s) 447, 494, 517, 520
HpyCH4IV ACGT 2 cut(s) 416, 515
HpyCH4V TGCA 2 cut(s) 202, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 4 cut(s) 213, 262, 309, 328
HpySE526I ACGT 2 cut(s) 416, 515
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 1 cut(s) 390
KroI GCCGGC 1 cut(s) 117
KroNI GCCGGC 1 cut(s) 119
KspAI GTTAAC 1 cut(s) 249
Kzo9I GATC 3 cut(s) 88, 347, 481
LmnI GCTCC 2 cut(s) 223, 452
LpnPI CCDG 7 cut(s) 131, 165, 220, 280, 315, 434, 480
Lsp1109I GCAGC 1 cut(s) 492
MaeI CTAG 2 cut(s) 275, 383
MaeII ACGT 2 cut(s) 416, 515
MaeIII GTNAC 1 cut(s) 99
MalI GATC 3 cut(s) 90, 349, 483
MboI GATC 3 cut(s) 88, 347, 481
MboII GAAGA 1 cut(s) 358
MfeI CAATTG 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 4 cut(s) 6, 240, 356, 377
MlyI GAGTC 2 cut(s) 105, 403
MnlI CCTC 5 cut(s) 271, 279, 304, 306, 367
MroNI GCCGGC 1 cut(s) 117
MseI TTAA 3 cut(s) 65, 239, 248
MspI CCGG 3 cut(s) 118, 152, 467
MspR9I CCNGG 1 cut(s) 153
MunI CAATTG 1 cut(s) 356
MvnI CGCG 7 cut(s) 298, 392, 407, 486, 494, 512, 520
MwoI GCNNNNNNNGC 1 cut(s) 330
NaeI GCCGGC 1 cut(s) 119
NciI CCSGG 1 cut(s) 153
NdeII GATC 3 cut(s) 88, 347, 481
NgoMIV GCCGGC 1 cut(s) 117
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 2 cut(s) 122, 150
NruI TCGCGA 1 cut(s) 520
PcsI WCGNNNNNNNCGW 2 cut(s) 451, 509
PdiI GCCGGC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 184
PflFI GACNNNGTC 1 cut(s) 415
PkrI GCNGC 4 cut(s) 300, 326, 488, 507
PleI GAGTC 2 cut(s) 105, 402
PpsI GAGTC 2 cut(s) 105, 402
PshBI ATTAAT 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 176
PspN4I GGNNCC 2 cut(s) 122, 150
PspPI GGNCC 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 415
RruI TCGCGA 1 cut(s) 520
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 65, 239, 248
SatI GCNGC 4 cut(s) 299, 325, 487, 506
Sau3AI GATC 3 cut(s) 88, 347, 481
Sau96I GGNCC 1 cut(s) 464
SchI GAGTC 2 cut(s) 105, 403
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 176
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 1 cut(s) 464
Sse9I AATT 4 cut(s) 6, 240, 356, 377
SsiI CCGC 9 cut(s) 94, 115, 283, 298, 325, 438, 486, 494, 510
SspMI CTAG 2 cut(s) 275, 383
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 2 cut(s) 50, 334
TaiI ACGT 2 cut(s) 419, 518
TasI AATT 4 cut(s) 6, 240, 356, 377
TatI WGTACW 1 cut(s) 430
TauI GCSGC 3 cut(s) 301, 327, 489
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 3 cut(s) 65, 239, 248
Tru9I TTAA 3 cut(s) 65, 239, 248
TscAI CASTG 1 cut(s) 536
TseI GCWGC 1 cut(s) 505
TspDTI ATGAA 2 cut(s) 35, 51
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 536
Tth111I GACNNNGTC 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 1 cut(s) 65
XapI RAATTY 2 cut(s) 6, 377
XspI CTAG 2 cut(s) 275, 383
ZraI GACGTC 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.