Rroxscaffold_70G00446410

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000070
OG0000295
Physical Location & Seq
Reverse (-)
61378 .. 62164
787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_70G00446410.1

Sequence Viewer

Length: 492 bp
ATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGAGCCCGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTATGAGTCCGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCCGGTGCCAGCAGCCGCGTCGGGGCATTCGTATTTCATAGTCGTAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGATCGGCGGATGTTACTTTTAGGACTCCGCCGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGAGTATGGTCGCAAGGCTGAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.51

Weight (kDa)

8.76

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000295)

Species Orthologous Gene IDs
pyrus_communis pycom2132g00010
rosa_roxburghii Rroxscaffold_101G00451080 Rroxscaffold_103G00450520 Rroxscaffold_105G00447130 Rroxscaffold_105G00447170 Rroxscaffold_107G00442620 Rroxscaffold_107G00442680 Rroxscaffold_107G00442720 Rroxscaffold_109G00451490 Rroxscaffold_111G00451540 Rroxscaffold_117G00451730 Rroxscaffold_119G00451820 Rroxscaffold_124G00451940 Rroxscaffold_129G00452080 Rroxscaffold_130G00452090 Rroxscaffold_133G00452140 Rroxscaffold_137G00452170 Rroxscaffold_139G00452220 Rroxscaffold_13G00448650 Rroxscaffold_13G00448660 Rroxscaffold_144G00452230 Rroxscaffold_147G00452260 Rroxscaffold_15G00447540 Rroxscaffold_15G00447550 Rroxscaffold_15G00447560 Rroxscaffold_16G00446230 Rroxscaffold_17G00435400 Rroxscaffold_17G00435490 Rroxscaffold_17G00435550 Rroxscaffold_17G00435630 Rroxscaffold_17G00435670 Rroxscaffold_17G00435740 Rroxscaffold_17G00435750 Rroxscaffold_17G00435820 Rroxscaffold_17G00435830 Rroxscaffold_17G00435890 Rroxscaffold_17G00435900 Rroxscaffold_1G00000120 Rroxscaffold_1G00000140 Rroxscaffold_1G00000170 Rroxscaffold_20G00445080 Rroxscaffold_21G00439530 Rroxscaffold_21G00439610 Rroxscaffold_22G00439860 Rroxscaffold_22G00439960 Rroxscaffold_24G00445010 Rroxscaffold_26G00447780 Rroxscaffold_27G00446620 Rroxscaffold_27G00446630 Rroxscaffold_28G00446940 Rroxscaffold_28G00446950 Rroxscaffold_28G00446960 Rroxscaffold_29G00441540 Rroxscaffold_29G00441550 Rroxscaffold_32G00442730 Rroxscaffold_32G00442850 Rroxscaffold_33G00439730 Rroxscaffold_36G00440150 Rroxscaffold_37G00445150 Rroxscaffold_38G00444610 Rroxscaffold_38G00444640 Rroxscaffold_39G00448190 Rroxscaffold_39G00448240 Rroxscaffold_39G00448250 Rroxscaffold_40G00447690 Rroxscaffold_42G00450450 Rroxscaffold_44G00440620 Rroxscaffold_44G00440650 Rroxscaffold_45G00438830 Rroxscaffold_45G00438970 Rroxscaffold_46G00448710 Rroxscaffold_46G00448750 Rroxscaffold_47G00444030 Rroxscaffold_51G00447880 Rroxscaffold_52G00439410 Rroxscaffold_55G00450110 Rroxscaffold_56G00450960 Rroxscaffold_57G00443090 Rroxscaffold_57G00443100 Rroxscaffold_58G00448990 Rroxscaffold_58G00449030 Rroxscaffold_58G00449040 Rroxscaffold_59G00442100 Rroxscaffold_59G00442110 Rroxscaffold_59G00442120 Rroxscaffold_59G00442130 Rroxscaffold_60G00448420 Rroxscaffold_61G00449960 Rroxscaffold_61G00449980 Rroxscaffold_62G00437950 Rroxscaffold_65G00445320 Rroxscaffold_65G00445330 Rroxscaffold_67G00448110 Rroxscaffold_67G00448180 Rroxscaffold_6G00387750 Rroxscaffold_70G00446410 Rroxscaffold_73G00439300 Rroxscaffold_74G00442880 Rroxscaffold_74G00442940 Rroxscaffold_75G00447420 Rroxscaffold_76G00448560 Rroxscaffold_77G00449170 Rroxscaffold_77G00449180 Rroxscaffold_77G00449190 Rroxscaffold_77G00449200 Rroxscaffold_81G00450460 Rroxscaffold_81G00450470 Rroxscaffold_82G00450790 Rroxscaffold_82G00450800 Rroxscaffold_84G00451000 Rroxscaffold_84G00451010 Rroxscaffold_90G00448800 Rroxscaffold_91G00442990 Rroxscaffold_91G00443020 Rroxscaffold_91G00443030 Rroxscaffold_91G00443050 Rroxscaffold_92G00446530 Rroxscaffold_93G00440920 Rroxscaffold_95G00449220 Rroxscaffold_96G00449120 Rroxscaffold_96G00449130 Rroxscaffold_96G00449140 Rroxscaffold_98G00451330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccII CGCG 2 cut(s) 89, 230
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 4 cut(s) 228, 404, 425, 428
AclWI GGATC 3 cut(s) 190, 203, 406
AcsI RAATTY 1 cut(s) 264
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 7 cut(s) 57, 111, 112, 162, 192, 205, 234
AjnI CCWGG 1 cut(s) 394
AjuI GAANNNNNNNTTGG 2 cut(s) 301, 333
Alw26I GTCTC 1 cut(s) 377
AlwI GGATC 3 cut(s) 190, 203, 406
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 80, 224
ApoI RAATTY 1 cut(s) 264
AseI ATTAAT 1 cut(s) 323
AspLEI GCGC 2 cut(s) 89, 91
AsuC2I CCSGG 2 cut(s) 142, 462
AsuHPI GGTGA 3 cut(s) 19, 26, 272
AvaI CYCGRG 1 cut(s) 50
BamHI GGATCC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 3 cut(s) 51, 147, 348
BbsI GAAGAC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 92, 236
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 1 cut(s) 396
BcnI CCSGG 2 cut(s) 142, 462
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 1 cut(s) 369
BisI GCNGC 4 cut(s) 81, 225, 228, 428
BlsI GCNGC 4 cut(s) 82, 226, 229, 429
Bme1390I CCNGG 3 cut(s) 142, 396, 462
BmeT110I CYCGRG 1 cut(s) 50
BmiI GGNNCC 4 cut(s) 48, 197, 219, 459
BmrFI CCNGG 3 cut(s) 142, 396, 462
BmsI GCATC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 357
BpuMI CCSGG 2 cut(s) 142, 462
Bsa29I ATCGAT 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 71, 308, 461
BsaWI WCCGGW 3 cut(s) 38, 155, 214
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 111, 112, 162, 192, 205, 234
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 358
BseDI CCNNGG 3 cut(s) 71, 308, 461
BseGI GGATG 3 cut(s) 67, 318, 412
BseLI CCNNNNNNNGG 7 cut(s) 57, 111, 112, 162, 192, 205, 234
BsePI GCGCGC 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 92, 236
Bsh1236I CGCG 2 cut(s) 89, 230
Bsh1285I CGRYCG 1 cut(s) 358
BshNI GGYRCC 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 358
BsiEI CGRYCG 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 5 cut(s) 39, 141, 156, 215, 461
BslI CCNNNNNNNGG 7 cut(s) 57, 111, 112, 162, 192, 205, 234
BsmAI GTCTC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 3 cut(s) 51, 147, 348
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 3 cut(s) 195, 355, 398
BspACI CCGC 4 cut(s) 228, 404, 425, 428
BspDI ATCGAT 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 38
BspFNI CGCG 2 cut(s) 89, 230
BspLI GGNNCC 4 cut(s) 48, 197, 219, 459
BspPI GGATC 3 cut(s) 190, 203, 406
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 71, 308, 461
BssHII GCGCGC 1 cut(s) 87
BssMI GATC 3 cut(s) 195, 355, 398
BssT1I CCWWGG 2 cut(s) 71, 308
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 85, 89, 222
BstENI CCTNNNNNAGG 1 cut(s) 190
BstF5I GGATG 3 cut(s) 67, 318, 412
BstFNI CGCG 2 cut(s) 89, 230
BstHHI GCGC 2 cut(s) 89, 91
BstKTI GATC 3 cut(s) 198, 358, 401
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 3 cut(s) 195, 355, 398
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 3 cut(s) 140, 394, 460
BstUI CGCG 2 cut(s) 89, 230
BstV1I GCAGC 2 cut(s) 92, 236
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 358
BsuTUI ATCGAT 1 cut(s) 358
BtsCI GGATG 3 cut(s) 67, 318, 412
Cac8I GCNNGC 3 cut(s) 85, 89, 222
CfoI GCGC 2 cut(s) 89, 91
ClaI ATCGAT 1 cut(s) 358
CseI GACGC 1 cut(s) 219
Csp6I GTAC 1 cut(s) 172
CviJI RGCY 6 cut(s) 49, 61, 145, 227, 346, 482
CviKI_1 RGCY 6 cut(s) 49, 61, 145, 227, 346, 482
CviQI GTAC 1 cut(s) 172
DpnI GATC 3 cut(s) 197, 357, 400
DpnII GATC 3 cut(s) 195, 355, 398
EciI GGCGGA 2 cut(s) 414, 419
Eco130I CCWWGG 2 cut(s) 71, 308
Eco24I GRGCYC 3 cut(s) 51, 147, 348
Eco88I CYCGRG 1 cut(s) 50
EcoNI CCTNNNNNAGG 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 71, 308
EcoT38I GRGCYC 3 cut(s) 51, 147, 348
ErhI CCWWGG 2 cut(s) 71, 308
FaiI YATR 6 cut(s) 150, 251, 278, 381, 437, 471
Fnu4HI GCNGC 4 cut(s) 81, 225, 228, 428
FokI GGATG 3 cut(s) 54, 325, 419
FriOI GRGCYC 3 cut(s) 51, 147, 348
Fsp4HI GCNGC 4 cut(s) 81, 225, 228, 428
FspBI CTAG 1 cut(s) 369
GlaI GCGC 2 cut(s) 88, 90
GluI GCNGC 4 cut(s) 81, 225, 228, 428
HapII CCGG 5 cut(s) 39, 141, 156, 215, 461
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 89, 91
Hin6I GCGC 2 cut(s) 87, 89
HinP1I GCGC 2 cut(s) 87, 89
HinfI GANTC 3 cut(s) 35, 152, 421
HpaII CCGG 5 cut(s) 39, 141, 156, 215, 461
HphI GGTGA 3 cut(s) 19, 26, 272
Hpy188III TCNNGA 3 cut(s) 39, 105, 328
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HspAI GCGC 2 cut(s) 87, 89
Kpn2I TCCGGA 1 cut(s) 38
Kzo9I GATC 3 cut(s) 195, 355, 398
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 9 cut(s) 52, 69, 154, 169, 228, 234, 381, 408, 474
Lsp1109I GCAGC 2 cut(s) 92, 236
LweI GCATC 1 cut(s) 377
MaeI CTAG 1 cut(s) 369
MaeIII GTNAC 4 cut(s) 7, 14, 121, 409
MalI GATC 3 cut(s) 197, 357, 400
MboI GATC 3 cut(s) 195, 355, 398
MboII GAAGA 1 cut(s) 362
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 3 cut(s) 51, 147, 348
MluCI AATT 4 cut(s) 23, 93, 160, 264
MlyI GAGTC 2 cut(s) 161, 415
MnlI CCTC 4 cut(s) 37, 109, 186, 198
MroI TCCGGA 1 cut(s) 38
MseI TTAA 3 cut(s) 188, 323, 490
MspI CCGG 5 cut(s) 39, 141, 156, 215, 461
MspR9I CCNGG 3 cut(s) 142, 396, 462
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 396
MvnI CGCG 2 cut(s) 89, 230
MwoI GCNNNNNNNGC 1 cut(s) 86
NciI CCSGG 2 cut(s) 142, 462
NdeII GATC 3 cut(s) 195, 355, 398
NlaIV GGNNCC 4 cut(s) 48, 197, 219, 459
NmuCI GTSAC 3 cut(s) 7, 14, 121
PauI GCGCGC 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 4 cut(s) 82, 226, 229, 429
Ple19I CGATCG 1 cut(s) 358
PleI GAGTC 2 cut(s) 160, 415
PpsI GAGTC 2 cut(s) 160, 415
PshBI ATTAAT 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 4 cut(s) 48, 197, 219, 459
PsuI RGATCY 1 cut(s) 195
PteI GCGCGC 1 cut(s) 87
PvuI CGATCG 1 cut(s) 358
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SaqAI TTAA 3 cut(s) 188, 323, 490
SatI GCNGC 4 cut(s) 81, 225, 228, 428
Sau3AI GATC 3 cut(s) 195, 355, 398
SchI GAGTC 2 cut(s) 161, 415
ScrFI CCNGG 3 cut(s) 142, 396, 462
SduI GDGCHC 3 cut(s) 51, 147, 348
SetI ASST 3 cut(s) 120, 262, 435
SfaNI GCATC 1 cut(s) 377
Sse9I AATT 4 cut(s) 23, 93, 160, 264
SsiI CCGC 4 cut(s) 228, 404, 425, 428
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 3 cut(s) 140, 394, 460
StyI CCWWGG 2 cut(s) 71, 308
TaaI ACNGT 1 cut(s) 365
TaqI TCGA 3 cut(s) 33, 348, 358
TasI AATT 4 cut(s) 23, 93, 160, 264
TatI WGTACW 1 cut(s) 171
TauI GCSGC 2 cut(s) 230, 430
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 3 cut(s) 188, 323, 490
Tru9I TTAA 3 cut(s) 188, 323, 490
TseFI GTSAC 3 cut(s) 7, 14, 121
TseI GCWGC 2 cut(s) 80, 224
Tsp45I GTSAC 3 cut(s) 7, 14, 121
TspDTI ATGAA 3 cut(s) 238, 293, 309
TspGWI ACGGA 1 cut(s) 33
VspI ATTAAT 1 cut(s) 323
XagI CCTNNNNNAGG 1 cut(s) 190
XapI RAATTY 1 cut(s) 264
XspI CTAG 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.