pycom2749g00060

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002749
OG0005757
Physical Location & Seq
Forward (+)
26744 .. 30193
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGCTGTCAAATTGTCTTATATATATATATATTTTTTGGTTTTGGGACGTGATTTTTTGTGTAGTTGTTCGCACGCTTACCGGTCACAGATCCAATTGTATATCTGTGGACTTTCATCCTTTTGGGGAGTTCTTTGCATCTGGCTCCTTAGATACAAATCTCAAAATATGGGATATCAGGAAGAAGGGGTGTATTCACACATACAAAGGCCATACTCGAGGGGTCAATGCTATCAGATTTACCCCAGATGGACGTTGGGTTGTTTCTGGTGGAGAGGACAACACTGTGAAGTTGTGGGACCTGACCGCTGGAAAGCTGCTGCATGACTTCAAGTGTCATGAGGGCCAGGTTCAGTGCATAGACTTTCATCCCCACGAGTTTCTGCTAGCAACAGGTTCAGCAGATAGAACTGTCAAATTTTGGGACCTTGAAACCTTCGAGCTAATTGGCTCTGCTGGACCTGAGACAACTGGAGTGCGTTCTTTGACTTTTAGCCCTGATGGGAGAGCCCTACTCTGTGGATTGCATGAAAGTTTAAAGGTTTTCTCGTGGGAGCCAATAAGGTGCCATGATGCTGTGGAGGTGGGATGGTCTCGATTGTCAGATCTTAATGTTCATGAGGGAAAGCTTCTTGGATGCTCTTACAATCAGAGTTGTGTTGGAGTGTGGGTTGTAGACATCTCGCGCATTGAACCGTATGCAATTGGTAATGCCAGCAGATTGAGTGGGCATTCAGAATCTAAATCATTGTCTGGTGGGAATCTCTCTGTGCTCAATGAGAACGCTGCTAAATCTAGTTTGGGCCGGTTATCTGTTTCACAAAATTCAGATTCTTTAGTGAAGGAAACAAAATCTCTTGGAAGGTTATCAGTTTCTCAGAATTCAGATCTCGTTAAGGAGCCAAAAATTATTGCATCCACAGGAATTGTTCCTGGTGCTCCTCAAAGGATCAGTATGAATTCCAGTCCAAAAACTATTGTGGCTAGTTCAGTGGCAGTTCCTAATGCATCAACCCTGAAGAGGAGTTCTACTAAGACCCATTCAACAGCTAATATTCCAACTTTTAACAAGTCTGATGTCATACCTGTAATTGTACCAAGAACTAGTATGAGGTCAGAGCTGTCAGCTGAGTCTAGAAAAGAGGTTAGCGTTGCTGGAAGACCACTGGCATTTTCTTTGCAGTCAAAGGCAACTGATTTCCGGAAGTTGTCCAATAGCAGAGAAGAAGTGGATAAGCCAGCTGTCTCTTCGGTGTCTGAATCCCCTGCTTCTAAGGCTATTGAATTAAGCAGTGCTGCAGATAAGAATGGCTTCTCAACAGTTTTAAGTTCAACTCAGGGAATACATGCTGAAAAGAACACAAAAGATGATATATATTTTGGGCCTGGGAAGCATGAAACTAGTTCAATGATGGCGCCAACTGCAAGTTACCAGCATGAAAGTTGTATGTATCTAATCTTTAGTAGAATGCTTTCCACTCATCCAATATATTCAATATATCGCCATTTAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

505

Amino Acids

55.23

Weight (kDa)

8.53

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 14 - 179 1e-24 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 14 - 104 5.9e-16 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 14 - 182 1.3e-34 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 15 - 103 1.7e-11 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 16 - 122 1.9e-07 SCAP Beta-propeller
WD40_Prp19 PF24814 21 - 178 1.1e-30 Prp19 WD40 domain
WD40 PF00400 22 - 58 3e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 22 - 113 1.5e-23 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 24 - 70 3.3e-07 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 24 - 110 3.4e-07 TEP-1 second beta-propeller
WDR55 PF24796 24 - 187 9e-17 WDR55
Beta-prop_WDR5 PF25175 25 - 187 2.6e-37 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 27 - 101 2.6e-07 MABP1/WDR62 second WD40 domain
Beta-prop_Aladin PF25460 28 - 119 1e-06 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 30 - 123 5.5e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SPT8 PF23798 43 - 125 5.4e-06 SPT8 beta propeller
Beta-prop_WDR36-Utp21_1st PF25171 43 - 99 3.2e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 58 - 131 4.4e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 59 - 171 3e-08 MABP1/WDR62 first WD40 domain
WD40 PF00400 64 - 100 6.4e-14 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 66 - 147 9.8e-12 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 66 - 146 6.8e-08 TEP-1 second beta-propeller
EIF3I PF24805 69 - 177 1.2e-16 EIF3I
ANAPC4_WD40 PF12894 71 - 123 1.5e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 73 - 144 2.7e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 73 - 148 8.5e-12 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 75 - 181 3.1e-06 WDR90, 4th beta-propeller
WD40_Gbeta PF25391 81 - 190 4.4e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 92 - 173 2e-11 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 96 - 147 5.4e-07 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 96 - 184 9.3e-07 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 99 - 175 1.9e-14 THOC3 beta-propeller domain
WD40 PF00400 104 - 142 3.2e-10 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 192 2.6e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 118 - 226 1.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 564, 1414
AccI GTMKAC 1 cut(s) 675
AccII CGCG 1 cut(s) 685
AccIII TCCGGA 1 cut(s) 1200
AciI CCGC 1 cut(s) 306
AclWI GGATC 2 cut(s) 84, 956
AcsI RAATTY 4 cut(s) 416, 823, 880, 958
AcuI CTGAAG 1 cut(s) 1037
AcyI GRCGYC 1 cut(s) 1415
AfaI GTAC 1 cut(s) 1095
AfiI CCNNNNNNNGG 1 cut(s) 1020
AgeI ACCGGT 1 cut(s) 80
AgsI TTSAA 8 cut(s) 331, 431, 692, 1044, 1283, 1332, 1407, 1494
AhlI ACTAGT 2 cut(s) 1103, 1400
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 3 cut(s) 345, 931, 1384
AluBI AGCT 7 cut(s) 316, 442, 628, 1049, 1120, 1127, 1241
AluI AGCT 7 cut(s) 316, 442, 628, 1049, 1120, 1127, 1241
Alw21I GWGCWC 2 cut(s) 774, 940
Alw26I GTCTC 3 cut(s) 458, 597, 1249
AlwI GGATC 2 cut(s) 84, 956
Ama87I CYCGRG 1 cut(s) 216
Aor13HI TCCGGA 1 cut(s) 1200
AoxI GGCC 4 cut(s) 208, 343, 802, 1382
ApeKI GCWGC 4 cut(s) 316, 319, 785, 1295
ApoI RAATTY 4 cut(s) 416, 823, 880, 958
AsiGI ACCGGT 1 cut(s) 80
Asp700I GAANNNNTTC 2 cut(s) 1310, 1471
AspLEI GCGC 2 cut(s) 687, 1417
AspS9I GGNCC 6 cut(s) 298, 343, 424, 458, 802, 1382
AsuNHI GCTAGC 1 cut(s) 385
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 3 cut(s) 298, 424, 458
BanI GGYRCC 2 cut(s) 564, 1414
BanII GRGCYC 1 cut(s) 511
BauI CACGAG 2 cut(s) 374, 547
BbsI GAAGAC 1 cut(s) 1165
Bbv12I GWGCWC 2 cut(s) 774, 940
BbvI GCAGC 4 cut(s) 303, 306, 772, 1282
BccI CCATC 4 cut(s) 242, 494, 582, 1405
BciT130I CCWGG 3 cut(s) 347, 933, 1386
BcoDI GTCTC 3 cut(s) 458, 597, 1249
BcuI ACTAGT 2 cut(s) 1103, 1400
BfaI CTAG 6 cut(s) 386, 795, 984, 1104, 1134, 1401
BfmI CTRYAG 1 cut(s) 1296
BfoI RGCGCY 1 cut(s) 1418
BglII AGATCT 2 cut(s) 604, 886
BisI GCNGC 4 cut(s) 317, 320, 786, 1296
BlsI GCNGC 4 cut(s) 318, 321, 787, 1297
Bme1390I CCNGG 3 cut(s) 347, 933, 1386
Bme18I GGWCC 3 cut(s) 298, 424, 458
BmeT110I CYCGRG 1 cut(s) 216
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 6 cut(s) 298, 343, 424, 458, 802, 1382
BmiI GGNNCC 7 cut(s) 145, 299, 425, 555, 566, 900, 1416
BmrFI CCNGG 3 cut(s) 347, 933, 1386
BmsI GCATC 5 cut(s) 146, 562, 626, 923, 1016
BmtI GCTAGC 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 1165
BplI GAGNNNNNCTC 2 cut(s) 498, 530
BpmI CTGGAG 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 1415
BsaI GGTCTC 1 cut(s) 597
BsaJI CCNNGG 1 cut(s) 1385
BsaWI WCCGGW 2 cut(s) 80, 1200
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 1 cut(s) 1020
Bse118I RCCGGY 2 cut(s) 80, 804
Bse1I ACTGG 3 cut(s) 475, 963, 1170
Bse8I GATNNNNATC 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 1200
BseBI CCWGG 3 cut(s) 347, 933, 1386
BseDI CCNNGG 1 cut(s) 1385
BseGI GGATG 6 cut(s) 115, 367, 593, 641, 914, 1480
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 1020
BseMII CTCAG 4 cut(s) 453, 890, 1119, 1349
BseNI ACTGG 3 cut(s) 475, 963, 1170
BseRI GAGGAG 2 cut(s) 930, 1036
BseXI GCAGC 4 cut(s) 303, 306, 772, 1282
Bsh1236I CGCG 1 cut(s) 685
BshFI GGCC 4 cut(s) 210, 345, 804, 1384
BshNI GGYRCC 2 cut(s) 564, 1414
BshTI ACCGGT 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 774, 940
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 3 cut(s) 81, 805, 1201
BslFI GGGAC 3 cut(s) 59, 311, 437
BslI CCNNNNNNNGG 1 cut(s) 1020
BsmAI GTCTC 3 cut(s) 458, 597, 1249
BsmFI GGGAC 3 cut(s) 59, 311, 437
BsmI GAATGC 2 cut(s) 730, 1473
BsnI GGCC 4 cut(s) 210, 345, 804, 1384
Bso31I GGTCTC 1 cut(s) 597
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 3 cut(s) 511, 774, 940
Bsp13I TCCGGA 1 cut(s) 1200
Bsp143I GATC 4 cut(s) 89, 604, 886, 948
BspACI CCGC 1 cut(s) 306
BspANI GGCC 4 cut(s) 210, 345, 804, 1384
BspCNI CTCAG 4 cut(s) 454, 889, 1120, 1348
BspEI TCCGGA 1 cut(s) 1200
BspFNI CGCG 1 cut(s) 685
BspHI TCATGA 2 cut(s) 337, 616
BspLI GGNNCC 7 cut(s) 145, 299, 425, 555, 566, 900, 1416
BspMAI CTGCAG 1 cut(s) 1300
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 2 cut(s) 84, 956
BspT107I GGYRCC 2 cut(s) 564, 1414
BspTNI GGTCTC 1 cut(s) 597
BsrFI RCCGGY 2 cut(s) 80, 804
BsrI ACTGG 3 cut(s) 475, 963, 1170
BssAI RCCGGY 2 cut(s) 80, 804
BssECI CCNNGG 1 cut(s) 1385
BssMI GATC 4 cut(s) 89, 604, 886, 948
BssNI GRCGYC 1 cut(s) 1415
BssSI CACGAG 2 cut(s) 374, 547
Bst2BI CACGAG 2 cut(s) 374, 547
Bst2UI CCWGG 3 cut(s) 347, 933, 1386
Bst4CI ACNGT 4 cut(s) 286, 412, 696, 1321
Bst6I CTCTTC 2 cut(s) 1013, 1252
BstACI GRCGYC 1 cut(s) 1415
BstC8I GCNNGC 4 cut(s) 74, 387, 715, 1239
BstDEI CTNAG 7 cut(s) 148, 462, 876, 1032, 1128, 1272, 1335
BstF5I GGATG 6 cut(s) 115, 367, 593, 641, 914, 1480
BstFNI CGCG 1 cut(s) 685
BstH2I RGCGCY 1 cut(s) 1418
BstHHI GCGC 2 cut(s) 687, 1417
BstKTI GATC 4 cut(s) 92, 607, 889, 951
BstMAI GTCTC 3 cut(s) 458, 597, 1249
BstMBI GATC 4 cut(s) 89, 604, 886, 948
BstMWI GCNNNNNNNGC 3 cut(s) 1274, 1390, 1421
BstNI CCWGG 3 cut(s) 347, 933, 1386
BstNSI RCATGY 1 cut(s) 1349
BstSCI CCNGG 3 cut(s) 345, 931, 1384
BstSFI CTRYAG 1 cut(s) 1296
BstUI CGCG 1 cut(s) 685
BstV1I GCAGC 4 cut(s) 303, 306, 772, 1282
BstV2I GAAGAC 1 cut(s) 1165
BstX2I RGATCY 3 cut(s) 89, 604, 886
BstYI RGATCY 3 cut(s) 89, 604, 886
BsuRI GGCC 4 cut(s) 210, 345, 804, 1384
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 6 cut(s) 115, 367, 593, 641, 914, 1480
BtsI GCAGTG 1 cut(s) 1297
BtsIMutI CAGTG 5 cut(s) 282, 359, 996, 1163, 1297
Cac8I GCNNGC 4 cut(s) 74, 387, 715, 1239
CciI TCATGA 2 cut(s) 337, 616
CfoI GCGC 2 cut(s) 687, 1417
Cfr10I RCCGGY 2 cut(s) 80, 804
Cfr13I GGNCC 6 cut(s) 298, 343, 424, 458, 802, 1382
Csp6I GTAC 1 cut(s) 1094
CspAI ACCGGT 1 cut(s) 80
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 8 cut(s) 323, 338, 527, 569, 617, 1346, 1394, 1436
CviQI GTAC 1 cut(s) 1094
DdeI CTNAG 7 cut(s) 148, 462, 876, 1032, 1128, 1272, 1335
DinI GGCGCC 1 cut(s) 1416
DpnI GATC 4 cut(s) 91, 606, 888, 950
DpnII GATC 4 cut(s) 89, 604, 886, 948
DraI TTTAAA 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 1013, 1252
EarI CTCTTC 2 cut(s) 1013, 1252
Eco24I GRGCYC 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 3 cut(s) 298, 424, 458
Eco57I CTGAAG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 298, 424
EcoRI GAATTC 2 cut(s) 880, 958
EcoRII CCWGG 3 cut(s) 345, 931, 1384
EcoRV GATATC 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 511
EgeI GGCGCC 1 cut(s) 1416
EheI GGCGCC 1 cut(s) 1416
FaeI CATG 8 cut(s) 326, 341, 530, 572, 620, 1349, 1397, 1439
FalI AAGNNNNNCTT 2 cut(s) 1295, 1327
FaqI GGGAC 3 cut(s) 59, 311, 437
FatI CATG 8 cut(s) 322, 337, 526, 568, 616, 1345, 1393, 1435
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 4 cut(s) 317, 320, 786, 1296
FokI GGATG 6 cut(s) 102, 354, 600, 648, 901, 1467
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 4 cut(s) 317, 320, 786, 1296
FspBI CTAG 6 cut(s) 386, 795, 984, 1104, 1134, 1401
GlaI GCGC 2 cut(s) 686, 1416
GluI GCNGC 4 cut(s) 317, 320, 786, 1296
GsuI CTGGAG 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 1418
HaeIII GGCC 4 cut(s) 210, 345, 804, 1384
HapII CCGG 3 cut(s) 81, 805, 1201
HhaI GCGC 2 cut(s) 687, 1417
Hin1I GRCGYC 1 cut(s) 1415
Hin1II CATG 8 cut(s) 326, 341, 530, 572, 620, 1349, 1397, 1439
Hin6I GCGC 2 cut(s) 685, 1415
HinP1I GCGC 2 cut(s) 685, 1415
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 5 cut(s) 737, 760, 830, 1130, 1259
HpaII CCGG 3 cut(s) 81, 805, 1201
Hpy166II GTNNAC 2 cut(s) 109, 676
Hpy188III TCNNGA 6 cut(s) 178, 338, 594, 617, 1134, 1201
Hpy8I GTNNAC 2 cut(s) 109, 676
HpyAV CCTTC 4 cut(s) 178, 445, 835, 855
HpyCH4III ACNGT 4 cut(s) 286, 412, 696, 1321
HpyCH4IV ACGT 2 cut(s) 48, 253
HpyF10VI GCNNNNNNNGC 3 cut(s) 1274, 1390, 1421
HpyF3I CTNAG 7 cut(s) 148, 462, 876, 1032, 1128, 1272, 1335
HpySE526I ACGT 2 cut(s) 48, 253
Hsp92I GRCGYC 1 cut(s) 1415
Hsp92II CATG 8 cut(s) 326, 341, 530, 572, 620, 1349, 1397, 1439
HspAI GCGC 2 cut(s) 685, 1415
KasI GGCGCC 1 cut(s) 1414
Kpn2I TCCGGA 1 cut(s) 1200
Kzo9I GATC 4 cut(s) 89, 604, 886, 948
LmnI GCTCC 4 cut(s) 149, 553, 898, 943
Lsp1109I GCAGC 4 cut(s) 303, 306, 772, 1282
LweI GCATC 5 cut(s) 146, 562, 626, 923, 1016
MaeI CTAG 6 cut(s) 386, 795, 984, 1104, 1134, 1401
MaeII ACGT 2 cut(s) 48, 253
MaeIII GTNAC 2 cut(s) 83, 1427
MalI GATC 4 cut(s) 91, 606, 888, 950
MboI GATC 4 cut(s) 89, 604, 886, 948
MboII GAAGA 5 cut(s) 193, 1030, 1170, 1235, 1239
MfeI CAATTG 2 cut(s) 94, 702
MflI RGATCY 3 cut(s) 89, 604, 886
MhlI GDGCHC 3 cut(s) 511, 774, 940
Mly113I GGCGCC 1 cut(s) 1415
MlyI GAGTC 1 cut(s) 1139
MmeI TCCRAC 2 cut(s) 640, 1082
MnlI CCTC 9 cut(s) 212, 268, 334, 574, 613, 951, 1014, 1104, 1135
Mph1103I ATGCAT 1 cut(s) 1009
MroI TCCGGA 1 cut(s) 1200
MroXI GAANNNNTTC 2 cut(s) 1310, 1471
MseI TTAA 6 cut(s) 536, 609, 894, 1065, 1286, 1325
MspA1I CMGCKG 3 cut(s) 308, 1127, 1241
MspI CCGG 3 cut(s) 81, 805, 1201
MspR9I CCNGG 3 cut(s) 347, 933, 1386
MunI CAATTG 2 cut(s) 94, 702
Mva1269I GAATGC 2 cut(s) 730, 1473
MvaI CCWGG 3 cut(s) 347, 933, 1386
MvnI CGCG 1 cut(s) 685
MwoI GCNNNNNNNGC 3 cut(s) 1274, 1390, 1421
NarI GGCGCC 1 cut(s) 1415
NdeII GATC 4 cut(s) 89, 604, 886, 948
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 8 cut(s) 326, 341, 530, 572, 620, 1349, 1397, 1439
NlaIV GGNNCC 7 cut(s) 145, 299, 425, 555, 566, 900, 1416
NmuCI GTSAC 1 cut(s) 83
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 1 cut(s) 1349
PaeR7I CTCGAG 1 cut(s) 216
PagI TCATGA 2 cut(s) 337, 616
PctI GAATGC 2 cut(s) 730, 1473
PdmI GAANNNNTTC 2 cut(s) 1310, 1471
PfeI GAWTC 4 cut(s) 737, 760, 830, 1259
PinAI ACCGGT 1 cut(s) 80
PkrI GCNGC 4 cut(s) 318, 321, 787, 1297
PleI GAGTC 1 cut(s) 1138
PluTI GGCGCC 1 cut(s) 1418
PpsI GAGTC 1 cut(s) 1138
PpuMI RGGWCCY 2 cut(s) 298, 424
Psp5II RGGWCCY 2 cut(s) 298, 424
Psp6I CCWGG 3 cut(s) 345, 931, 1384
PspGI CCWGG 3 cut(s) 345, 931, 1384
PspN4I GGNNCC 7 cut(s) 145, 299, 425, 555, 566, 900, 1416
PspPI GGNCC 6 cut(s) 298, 343, 424, 458, 802, 1382
PspPPI RGGWCCY 2 cut(s) 298, 424
PspXI VCTCGAGB 1 cut(s) 216
PstI CTGCAG 1 cut(s) 1300
PsuI RGATCY 3 cut(s) 89, 604, 886
PvuII CAGCTG 2 cut(s) 1127, 1241
RsaI GTAC 1 cut(s) 1095
RsaNI GTAC 1 cut(s) 1094
SaqAI TTAA 6 cut(s) 536, 609, 894, 1065, 1286, 1325
SatI GCNGC 4 cut(s) 317, 320, 786, 1296
Sau3AI GATC 4 cut(s) 89, 604, 886, 948
Sau96I GGNCC 6 cut(s) 298, 343, 424, 458, 802, 1382
SchI GAGTC 1 cut(s) 1139
ScrFI CCNGG 3 cut(s) 347, 933, 1386
SduI GDGCHC 3 cut(s) 511, 774, 940
SfaNI GCATC 5 cut(s) 146, 562, 626, 923, 1016
SfcI CTRYAG 1 cut(s) 1296
SfoI GGCGCC 1 cut(s) 1416
Sfr274I CTCGAG 1 cut(s) 216
SinI GGWCC 3 cut(s) 298, 424, 458
SlaI CTCGAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
SpeI ACTAGT 2 cut(s) 1103, 1400
SsiI CCGC 1 cut(s) 306
SspDI GGCGCC 1 cut(s) 1414
SspI AATATT 1 cut(s) 1054
SspMI CTAG 6 cut(s) 386, 795, 984, 1104, 1134, 1401
StyD4I CCNGG 3 cut(s) 345, 931, 1384
TaaI ACNGT 4 cut(s) 286, 412, 696, 1321
TaiI ACGT 2 cut(s) 51, 256
TaqI TCGA 3 cut(s) 217, 438, 595
TfiI GAWTC 4 cut(s) 737, 760, 830, 1259
Tru1I TTAA 6 cut(s) 536, 609, 894, 1065, 1286, 1325
Tru9I TTAA 6 cut(s) 536, 609, 894, 1065, 1286, 1325
TscAI CASTG 5 cut(s) 289, 359, 996, 1170, 1297
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 4 cut(s) 316, 319, 785, 1295
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 7 cut(s) 104, 356, 543, 605, 971, 1410, 1452
TspRI CASTG 5 cut(s) 289, 359, 996, 1170, 1297
VpaK11BI GGWCC 3 cut(s) 298, 424, 458
XapI RAATTY 4 cut(s) 416, 823, 880, 958
XbaI TCTAGA 1 cut(s) 1133
XceI RCATGY 1 cut(s) 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XhoI CTCGAG 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 2 cut(s) 1310, 1471
XspI CTAG 6 cut(s) 386, 795, 984, 1104, 1134, 1401
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.