Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

SuperScaffold_420

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

1669606 .. 1682791

Gene Structure 13186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGGTTGCCCAAAGAGGCAGCCCATGGGGGCTCCGATGTCTCTTCCTCTGCCTGGCCGTTCAATTTGCTCTGGAGGCGACCGCCGAGTACTTCAAGCCATTCAACGTCAGCTACGACCACCGAGCTCTAATCATCGACGGCAAGCGCCGCATGCTCGTCTCCGCCGGAATCCACTACCCCCGCGCTACTCCTGAGATGTGGCCTGATCTGATCGCAAAAAGCAAGGAAGGCGGGGTGGATGTGATCCAAACTTATGCATTTTGGAGCGGCCATGAGCCAGTGAGAGGACAGTACAACTTTGAAGGGAGATATGATATCGTCAAGTTTGCAAATTTAGTGGGAGCCAGTGGACTCTATCTCCATCTACGCATAGGGCCTTATGTCTGCGCTGAGTGGAACTTCGGTGGCTTCCCCGTGTGGTTGCGTGACATCCCTGGAATAGAGTTTCGAACAAACAATGCGCTTTTTAAGGAGGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTGATGCAAGAGGAAGAACTATTATCTTGGCAAGGTGGTCCAATTATCATGCTGCAGATTGAAAATGAATATGGAAATATTGAAGGGCAATTTGGTCAGAAAGGGAAAGAATATATTAAGTGGGCTGCTGAGATGGCTTTAGGTCTCGGTGCTGGGGTTCCATGGGTCATGTGCAAGCAAGTTGATGCTCCAGGAAGTATTATAGATGCATGCAATGGCTACTATTGTGATGGTTACAAGCCAAATTCCTATAACAAACCAACAATGTGGACAGAAGATTGGGATGGATGGTATGCATCATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCAGTAGCACGCTTTTACCAACGTGGAGGGAGCTTTCAGAATTATTATATGTATTTTGGTGGAACAAACTTTGGGCGGACTTCTGGAGGCCCGTTTTATATTACTAGTTATGATTATGATGCTCCAATTGATGAATATGGTCTGCTGAGTGAGCCAAAATGGGGACATTTGAAGGATCTACATGCTGCTATAAAGCTCTGCGAACCTGCTCTTGTTGCTGCTGATTCTCCGAATTATATAAAGTTGGGACCAAAACAAGAGGCACACGTGTACCGCGTGAATTCTCACACCGAAGGCCTGAACATTACTTTGTATGGAAGTCAAATCAGTTGCTCCGCATTCCTTGCAAACATTGATGAACATAAAGCAGTTTCTGTAACATTCCTTGGGCAAAAGTATAATCTACCACCATGGTCAGTCAGTATTTTGCCAGACTGCCGGAACGTGGTGTACAACACTGCCAAGGTTGGTGCACAAACTTCCATCAAAACAGTAGAGTTTGATTTGCCCCTTTATTCAGGTATTTCTTCACAGCAACAATTCATTACCAAGAATGATGATTTGTTTATCACCAAATCATGGATGACTGTAAAAGAGCCAGTTGGTGTTTGGAGTGAAAACAATTTTACGGTCCAAGGAATATTGGAGCATTTGAATGTGACAAAAGACCAGTCTGATTACTTATGGCATATAACCAGGATTTTTGTTTCAGAGGATGACATATCTTTTTGGGAGAAAAACAATATTAGCGCAGCAGTCTCAATTGATAGCATGCGCGATGTGTTGCGCGTATTTGTTAATGGCCAGCTTACAGGCAGTGTCATCGGTCACTGGGTCAAGGTGGAGCAGCCTGTTAAGTTTCTGAAGGGATACAATGATTTGGTGTTGTTAACTCAGACAGTGGGCTTGCAGAACTATGGTGCTTTCCTGGAGAAAGATGGGGCAGGATTTAGAGGACAGATTAAACTAACTGGCTTTAAAAATGGTGATATTGACTTTTCAAAGTTGCTATGGACTTACCAGGTTGGGCTTAAGGGCGAGTTTTTGAAAATATACACCATAGAAGAAAATGAAAAGGCAAGTTGGGCTGAGTTATCGCCAGATGATGATCGATCCACATTTATATGGTACAAGGTAAATAATCTCATTCGCATGTGTGAGGCATATATTACCATCTATTTTACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTTGATTTAGGAAGCATGGGTAAGGGCCAGGCTTGGGTCAACGGCCACCATATAGGAAGATATTGGACACTTGTTGCTCCAGAAGATGGATGTCCAGAAATCTGTGATTACCGTGGGGCGTACAACTCCGATAAGTGCTCATTTAATTGTGGGAAGCCTACTCAAACCTTGTACCATGTACCGAGGTCATGGTTACAGTCTTCAAGTAATTTACTTGTTATTTTGGAGGAAACTGGCGGGAATCCATTTGATATTTCAATCAAGTTGCGATCAGCCGGAGTCCTCTGTGCTCAAGTGTCAGAGTCTCACTACCCACCTGTCCAGAAGTGGTTCAATCCAGACTCTGTGGATGAAAAAATTACAGTAAACGATCTGACACCAGAAATGCACTTGCAATGTCAAGATGGGTTTACGATATCTTCCATAGAATTTGCCAGCTATGGAACACCTCAAGGTAGCTGCCAGAAGTTTTCTATGGGAAATTGCCATGCAACCAATTCATCGTCCATTGTCTCCAAGAGTTGCCTGGGAAAGAATAGCTGTTCAGTTGAAATATCAAATATCAGTTTTGGAGGTGACCCATGCCGAGGCGTTGTTAAGACGTTGGCAGTTGAGGCAAGATGCAGGTCATCATCAGATGTTGGTTTGTCTCAGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

911

Amino Acids

102.1

Weight (kDa)

5.6

Isoelectric Point (pI)

39.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	43 - 347	3.5e-115	Glycosyl hydrolases family 35
GHD	PF17834	389 - 440	1.3e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	675 - 761	3.1e-22	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	822 - 899	2.2e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1066, 2688
AccB7I	CCANNNNNTGG	2 cut(s)	1431, 2159
AccBSI	CCGCTC	1 cut(s)	267
AccII	CGCG	4 cut(s)	183, 1128, 1629, 1641
AclWI	GGATC	5 cut(s)	238, 513, 1035, 1959, 2057
AcoI	YGGCCR	4 cut(s)	54, 268, 1654, 2116
AcsI	RAATTY	4 cut(s)	331, 751, 1132, 2501
AcuI	CTGAAG	1 cut(s)	1736
AcvI	CACGTG	1 cut(s)	1120
AfaI	GTAC	8 cut(s)	89, 293, 1124, 1304, 1982, 2195, 2246, 2253
AfiI	CCNNNNNNNGG	8 cut(s)	52, 284, 1013, 1297, 1431, 2107, 2159, 2660
AflII	CTTAAG	1 cut(s)	1883
AflIII	ACRYGT	2 cut(s)	1117, 1119
AhlI	ACTAGT	1 cut(s)	956
AjnI	CCWGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
AjuI	GAANNNNNNNTTGG	2 cut(s)	582, 614
AluBI	AGCT	8 cut(s)	111, 125, 885, 1048, 1660, 2511, 2532, 2613
AluI	AGCT	8 cut(s)	111, 125, 885, 1048, 1660, 2511, 2532, 2613
Alw21I	GWGCWC	4 cut(s)	127, 1327, 2213, 2365
Alw26I	GTCTC	7 cut(s)	44, 163, 656, 1615, 2382, 2590, 2727
Alw44I	GTGCAC	1 cut(s)	1323
AlwI	GGATC	5 cut(s)	238, 513, 1035, 1959, 2057
AlwNI	CAGNNNCTG	2 cut(s)	1226, 2417
ApaLI	GTGCAC	1 cut(s)	1323
ApeKI	GCWGC	9 cut(s)	18, 481, 559, 632, 1037, 1070, 1604, 1699, 2532
ApoI	RAATTY	4 cut(s)	331, 751, 1132, 2501
ArsI	GACNNNNNNTTYG	2 cut(s)	1508, 1540
Asp700I	GAANNNNTTC	1 cut(s)	2402
AspLEI	GCGC	7 cut(s)	147, 185, 389, 463, 1604, 1629, 1641
AspS9I	GGNCC	6 cut(s)	374, 545, 941, 1100, 1483, 2098
AsuHPI	GGTGA	3 cut(s)	1414, 1850, 2660
AsuII	TTCGAA	1 cut(s)	448
AvaII	GGWCC	3 cut(s)	545, 1100, 1483
BaeGI	GKGCMC	1 cut(s)	1327
BaeI	ACNNNNGTAYC	2 cut(s)	1106, 1139
BalI	TGGCCA	1 cut(s)	1656
BanII	GRGCYC	2 cut(s)	33, 127
BbrPI	CACGTG	1 cut(s)	1120
BbsI	GAAGAC	1 cut(s)	2265
Bbv12I	GWGCWC	4 cut(s)	127, 1327, 2213, 2365
BbvI	GCAGC	9 cut(s)	30, 493, 546, 619, 1024, 1057, 1616, 1711, 2519
BceAI	ACGGC	3 cut(s)	41, 154, 2131
BcgI	CGANNNNNNTGC	2 cut(s)	1657, 1691
BciT130I	CCWGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
BciVI	GTATCC	1 cut(s)	1715
BcoDI	GTCTC	7 cut(s)	44, 163, 656, 1615, 2382, 2590, 2727
BcuI	ACTAGT	1 cut(s)	956
BfaI	CTAG	1 cut(s)	957
BfmI	CTRYAG	1 cut(s)	560
BfoI	RGCGCY	1 cut(s)	148
BfrI	CTTAAG	1 cut(s)	1883
BfuAI	ACCTGC	2 cut(s)	1066, 2688
BfuI	GTATCC	1 cut(s)	1715
BglI	GCCNNNNNGGC	2 cut(s)	829, 2055
BglII	AGATCT	1 cut(s)	841
BmcAI	AGTACT	1 cut(s)	89
Bme1390I	CCNGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
Bme18I	GGWCC	3 cut(s)	545, 1100, 1483
BmgT120I	GGNCC	6 cut(s)	374, 545, 941, 1100, 1483, 2098
BmiI	GGNNCC	4 cut(s)	32, 343, 666, 1101
BmrFI	CCNGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
BmrI	ACTGGG	1 cut(s)	1693
BmsI	GCATC	7 cut(s)	468, 501, 682, 703, 812, 961, 2684
BmuI	ACTGGG	1 cut(s)	1693
BpiI	GAAGAC	1 cut(s)	2265
BpmI	CTGGAG	5 cut(s)	92, 681, 957, 1802, 2136
Bpu14I	TTCGAA	1 cut(s)	448
BpuEI	CTTGAG	2 cut(s)	2349, 2508
Bsa29I	ATCGAT	1 cut(s)	1963
BsaAI	YACGTR	1 cut(s)	1120
BsaBI	GATNNNNATC	1 cut(s)	1959
BsaI	GGTCTC	1 cut(s)	656
BsaXI	ACNNNNNCTCC	2 cut(s)	342, 372
Bsc4I	CCNNNNNNNGG	8 cut(s)	52, 284, 1013, 1297, 1431, 2107, 2159, 2660
Bse1I	ACTGG	8 cut(s)	278, 345, 1451, 1522, 1688, 1828, 2066, 2311
Bse3DI	GCAATG	2 cut(s)	727, 2474
Bse8I	GATNNNNATC	1 cut(s)	1959
BseBI	CCWGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
BseCI	ATCGAT	1 cut(s)	1963
BseGI	GGATG	8 cut(s)	244, 429, 796, 800, 1440, 1573, 2168, 2428
BseJI	GATNNNNATC	1 cut(s)	1959
BseLI	CCNNNNNNNGG	8 cut(s)	52, 284, 1013, 1297, 1431, 2107, 2159, 2660
BseMI	GCAATG	2 cut(s)	727, 2474
BseMII	CTCAG	6 cut(s)	183, 381, 627, 989, 1760, 1932
BseNI	ACTGG	8 cut(s)	278, 345, 1451, 1522, 1688, 1828, 2066, 2311
BseRI	GAGGAG	1 cut(s)	488
BseSI	GKGCMC	1 cut(s)	1327
BseXI	GCAGC	9 cut(s)	30, 493, 546, 619, 1024, 1057, 1616, 1711, 2519
BseYI	CCCAGC	1 cut(s)	659
Bsh1236I	CGCG	4 cut(s)	183, 1128, 1629, 1641
Bsh1285I	CGRYCG	1 cut(s)	81
BshVI	ATCGAT	1 cut(s)	1963
BsiEI	CGRYCG	1 cut(s)	81
BsiHKAI	GWGCWC	4 cut(s)	127, 1327, 2213, 2365
BsiSI	CCGG	3 cut(s)	165, 1291, 2349
BslFI	GGGAC	2 cut(s)	1029, 1113
BslI	CCNNNNNNNGG	8 cut(s)	52, 284, 1013, 1297, 1431, 2107, 2159, 2660
BsmAI	GTCTC	7 cut(s)	44, 163, 656, 1615, 2382, 2590, 2727
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	2 cut(s)	1029, 1113
BsmI	GAATGC	1 cut(s)	1190
Bso31I	GGTCTC	1 cut(s)	656
Bsp119I	TTCGAA	1 cut(s)	448
Bsp1286I	GDGCHC	5 cut(s)	33, 127, 1327, 2213, 2365
Bsp1407I	TGTACA	1 cut(s)	1302
Bsp19I	CCATGG	3 cut(s)	23, 668, 1262
BspCNI	CTCAG	6 cut(s)	184, 382, 628, 990, 1759, 1933
BspDI	ATCGAT	1 cut(s)	1963
BspFNI	CGCG	4 cut(s)	183, 1128, 1629, 1641
BspLI	GGNNCC	4 cut(s)	32, 343, 666, 1101
BspMAI	CTGCAG	1 cut(s)	564
BspMI	ACCTGC	2 cut(s)	1066, 2688
BspPI	GGATC	5 cut(s)	238, 513, 1035, 1959, 2057
BspT104I	TTCGAA	1 cut(s)	448
BspTI	CTTAAG	1 cut(s)	1883
BspTNI	GGTCTC	1 cut(s)	656
BsrBI	CCGCTC	1 cut(s)	267
BsrDI	GCAATG	2 cut(s)	727, 2474
BsrGI	TGTACA	1 cut(s)	1302
BsrI	ACTGG	8 cut(s)	278, 345, 1451, 1522, 1688, 1828, 2066, 2311
BssT1I	CCWWGG	6 cut(s)	23, 668, 1237, 1262, 1314, 1486
Bst2UI	CCWGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
Bst4CI	ACNGT	8 cut(s)	291, 1345, 1441, 1483, 1753, 2186, 2271, 2437
Bst6I	CTCTTC	1 cut(s)	47
BstAFI	CTTAAG	1 cut(s)	1883
BstAPI	GCANNNNNTGC	1 cut(s)	1196
BstAUI	TGTACA	1 cut(s)	1302
BstBAI	YACGTR	1 cut(s)	1120
BstBI	TTCGAA	1 cut(s)	448
BstDEI	CTNAG	7 cut(s)	192, 390, 636, 998, 1746, 1941, 2724
BstDSI	CCRYGG	4 cut(s)	23, 668, 1262, 2185
BstEII	GGTNACC	1 cut(s)	2648
BstF5I	GGATG	8 cut(s)	244, 429, 796, 800, 1440, 1573, 2168, 2428
BstFNI	CGCG	4 cut(s)	183, 1128, 1629, 1641
BstH2I	RGCGCY	1 cut(s)	148
BstHHI	GCGC	7 cut(s)	147, 185, 389, 463, 1604, 1629, 1641
BstMAI	GTCTC	7 cut(s)	44, 163, 656, 1615, 2382, 2590, 2727
BstMCI	CGRYCG	1 cut(s)	81
BstNI	CCWGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
BstNSI	RCATGY	5 cut(s)	154, 720, 1037, 1627, 2008
BstPI	GGTNACC	1 cut(s)	2648
BstSCI	CCNGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
BstSFI	CTRYAG	1 cut(s)	560
BstSLI	GKGCMC	1 cut(s)	1327
BstUI	CGCG	4 cut(s)	183, 1128, 1629, 1641
BstV1I	GCAGC	9 cut(s)	30, 493, 546, 619, 1024, 1057, 1616, 1711, 2519
BstV2I	GAAGAC	1 cut(s)	2265
BstX2I	RGATCY	4 cut(s)	505, 841, 1027, 2062
BstXI	CCANNNNNNTGG	1 cut(s)	774
BstYI	RGATCY	4 cut(s)	505, 841, 1027, 2062
Bsu15I	ATCGAT	1 cut(s)	1963
BsuI	GTATCC	1 cut(s)	1715
BsuTUI	ATCGAT	1 cut(s)	1963
BtgI	CCRYGG	4 cut(s)	23, 668, 1262, 2185
BtgZI	GCGATG	1 cut(s)	1644
BtsCI	GGATG	8 cut(s)	244, 429, 796, 800, 1440, 1573, 2168, 2428
BtsI	GCAGTG	2 cut(s)	1308, 1675
BtsIMutI	CAGTG	6 cut(s)	285, 352, 1308, 1675, 1681, 1758
BveI	ACCTGC	2 cut(s)	1066, 2688
CaiI	CAGNNNCTG	2 cut(s)	1226, 2417
CfoI	GCGC	7 cut(s)	147, 185, 389, 463, 1604, 1629, 1641
Cfr13I	GGNCC	6 cut(s)	374, 545, 941, 1100, 1483, 2098
ClaI	ATCGAT	1 cut(s)	1963
CsiI	ACCWGGT	1 cut(s)	1872
Csp6I	GTAC	8 cut(s)	88, 292, 1123, 1303, 1981, 2194, 2245, 2252
CspCI	CAANNNNNGTGG	2 cut(s)	318, 353
CviQI	GTAC	8 cut(s)	88, 292, 1123, 1303, 1981, 2194, 2245, 2252
DdeI	CTNAG	7 cut(s)	192, 390, 636, 998, 1746, 1941, 2724
DraI	TTTAAA	1 cut(s)	1831
EaeI	YGGCCR	4 cut(s)	54, 268, 1654, 2116
Eam1104I	CTCTTC	1 cut(s)	47
EarI	CTCTTC	1 cut(s)	47
EciI	GGCGGA	2 cut(s)	151, 943
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	6 cut(s)	23, 668, 1237, 1262, 1314, 1486
Eco147I	AGGCCT	2 cut(s)	832, 1149
Eco24I	GRGCYC	2 cut(s)	33, 127
Eco31I	GGTCTC	1 cut(s)	656
Eco32I	GATATC	2 cut(s)	316, 2490
Eco47I	GGWCC	3 cut(s)	545, 1100, 1483
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	1 cut(s)	1736
Eco72I	CACGTG	1 cut(s)	1120
Eco91I	GGTNACC	1 cut(s)	2648
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	1 cut(s)	374
EcoO65I	GGTNACC	1 cut(s)	2648
EcoRI	GAATTC	1 cut(s)	1132
EcoRII	CCWGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
EcoRV	GATATC	2 cut(s)	316, 2490
EcoT14I	CCWWGG	6 cut(s)	23, 668, 1237, 1262, 1314, 1486
EcoT22I	ATGCAT	3 cut(s)	259, 718, 805
EcoT38I	GRGCYC	2 cut(s)	33, 127
ErhI	CCWWGG	6 cut(s)	23, 668, 1237, 1262, 1314, 1486
Esp3I	CGTCTC	1 cut(s)	163
FaqI	GGGAC	2 cut(s)	1029, 1113
FauI	CCCGC	3 cut(s)	188, 224, 2303
FokI	GGATG	8 cut(s)	251, 416, 803, 807, 1447, 1580, 2175, 2435
FriOI	GRGCYC	2 cut(s)	33, 127
FspBI	CTAG	1 cut(s)	957
GlaI	GCGC	7 cut(s)	146, 184, 388, 462, 1603, 1628, 1640
GsaI	CCCAGC	1 cut(s)	663
GsuI	CTGGAG	5 cut(s)	92, 681, 957, 1802, 2136
HaeII	RGCGCY	1 cut(s)	148
HapII	CCGG	3 cut(s)	165, 1291, 2349
HhaI	GCGC	7 cut(s)	147, 185, 389, 463, 1604, 1629, 1641
Hin6I	GCGC	7 cut(s)	145, 183, 387, 461, 1602, 1627, 1639
HinP1I	GCGC	7 cut(s)	145, 183, 387, 461, 1602, 1627, 1639
HincII	GTYRAC	2 cut(s)	1743, 2113
HindII	GTYRAC	2 cut(s)	1743, 2113
HinfI	GANTC	7 cut(s)	168, 351, 1076, 2314, 2352, 2375, 2414
HpaI	GTTAAC	1 cut(s)	1743
HpaII	CCGG	3 cut(s)	165, 1291, 2349
HphI	GGTGA	3 cut(s)	1414, 1850, 2660
Hpy166II	GTNNAC	9 cut(s)	350, 777, 1123, 1303, 1325, 1743, 2113, 2440, 2484
Hpy188III	TCNNGA	9 cut(s)	71, 191, 936, 2075, 2153, 2168, 2395, 2411, 2474
Hpy8I	GTNNAC	9 cut(s)	350, 777, 1123, 1303, 1325, 1743, 2113, 2440, 2484
Hpy99I	CGWCG	1 cut(s)	140
HpyAV	CCTTC	6 cut(s)	221, 296, 584, 1018, 1139, 1711
HpyCH4III	ACNGT	8 cut(s)	291, 1345, 1441, 1483, 1753, 2186, 2271, 2437
HpyCH4IV	ACGT	5 cut(s)	105, 874, 1119, 1296, 2675
HpyF3I	CTNAG	7 cut(s)	192, 390, 636, 998, 1746, 1941, 2724
HpySE526I	ACGT	5 cut(s)	105, 874, 1119, 1296, 2675
HspAI	GCGC	7 cut(s)	145, 183, 387, 461, 1602, 1627, 1639
KspAI	GTTAAC	1 cut(s)	1743
Lsp1109I	GCAGC	9 cut(s)	30, 493, 546, 619, 1024, 1057, 1616, 1711, 2519
LweI	GCATC	7 cut(s)	468, 501, 682, 703, 812, 961, 2684
MabI	ACCWGGT	1 cut(s)	1872
MaeI	CTAG	1 cut(s)	957
MaeII	ACGT	5 cut(s)	105, 874, 1119, 1296, 2675
MaeIII	GTNAC	7 cut(s)	425, 740, 1228, 1510, 1679, 2265, 2648
MbiI	CCGCTC	1 cut(s)	267
MfeI	CAATTG	2 cut(s)	978, 1614
MflI	RGATCY	4 cut(s)	505, 841, 1027, 2062
MhlI	GDGCHC	5 cut(s)	33, 127, 1327, 2213, 2365
MlsI	TGGCCA	1 cut(s)	1656
MluNI	TGGCCA	1 cut(s)	1656
MlyI	GAGTC	4 cut(s)	345, 2361, 2384, 2408
Mox20I	TGGCCA	1 cut(s)	1656
Mph1103I	ATGCAT	3 cut(s)	259, 718, 805
MroXI	GAANNNNTTC	1 cut(s)	2402
MscI	TGGCCA	1 cut(s)	1656
MslI	CAYNNNNRTG	3 cut(s)	1506, 1975, 2003
Msp20I	TGGCCA	1 cut(s)	1656
MspA1I	CMGCKG	1 cut(s)	484
MspCI	CTTAAG	1 cut(s)	1883
MspI	CCGG	3 cut(s)	165, 1291, 2349
MspR9I	CCNGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
MunI	CAATTG	2 cut(s)	978, 1614
Mva1269I	GAATGC	1 cut(s)	1190
MvaI	CCWGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
MvnI	CGCG	4 cut(s)	183, 1128, 1629, 1641
NcoI	CCATGG	3 cut(s)	23, 668, 1262
NlaIV	GGNNCC	4 cut(s)	32, 343, 666, 1101
NmeAIII	GCCGAG	2 cut(s)	109, 2684
NmuCI	GTSAC	4 cut(s)	425, 1510, 1679, 2648
NsiI	ATGCAT	3 cut(s)	259, 718, 805
NspI	RCATGY	5 cut(s)	154, 720, 1037, 1627, 2008
NspV	TTCGAA	1 cut(s)	448
PaeI	GCATGC	3 cut(s)	154, 720, 1627
PceI	AGGCCT	2 cut(s)	832, 1149
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 1125
PctI	GAATGC	1 cut(s)	1190
PdmI	GAANNNNTTC	1 cut(s)	2402
PfeI	GAWTC	3 cut(s)	168, 1076, 2314
PflMI	CCANNNNNTGG	2 cut(s)	1431, 2159
PfoI	TCCNGGA	2 cut(s)	697, 1779
PleI	GAGTC	4 cut(s)	345, 2360, 2383, 2408
PmaCI	CACGTG	1 cut(s)	1120
PmlI	CACGTG	1 cut(s)	1120
PpsI	GAGTC	4 cut(s)	345, 2360, 2383, 2408
Ppu21I	YACGTR	1 cut(s)	1120
Psp124BI	GAGCTC	1 cut(s)	127
Psp6I	CCWGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
PspCI	CACGTG	1 cut(s)	1120
PspEI	GGTNACC	1 cut(s)	2648
PspFI	CCCAGC	1 cut(s)	659
PspGI	CCWGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
PspN4I	GGNNCC	4 cut(s)	32, 343, 666, 1101
PspPI	GGNCC	6 cut(s)	374, 545, 941, 1100, 1483, 2098
PsrI	GAACNNNNNNTAC	2 cut(s)	1286, 1318
PstI	CTGCAG	1 cut(s)	564
PstNI	CAGNNNCTG	2 cut(s)	1226, 2417
PsuI	RGATCY	4 cut(s)	505, 841, 1027, 2062
RsaI	GTAC	8 cut(s)	89, 293, 1124, 1304, 1982, 2195, 2246, 2253
RsaNI	GTAC	8 cut(s)	88, 292, 1123, 1303, 1981, 2194, 2245, 2252
RseI	CAYNNNNRTG	3 cut(s)	1506, 1975, 2003
SacI	GAGCTC	1 cut(s)	127
Sau96I	GGNCC	6 cut(s)	374, 545, 941, 1100, 1483, 2098
ScaI	AGTACT	1 cut(s)	89
SchI	GAGTC	4 cut(s)	345, 2361, 2384, 2408
ScrFI	CCNGG	8 cut(s)	53, 435, 699, 1549, 1781, 1874, 2102, 2600
SduI	GDGCHC	5 cut(s)	33, 127, 1327, 2213, 2365
SexAI	ACCWGGT	1 cut(s)	1872
SfaNI	GCATC	7 cut(s)	468, 501, 682, 703, 812, 961, 2684
SfcI	CTRYAG	1 cut(s)	560
SfuI	TTCGAA	1 cut(s)	448
SinI	GGWCC	3 cut(s)	545, 1100, 1483
SmiMI	CAYNNNNRTG	3 cut(s)	1506, 1975, 2003
SmlI	CTYRAG	3 cut(s)	1883, 2364, 2523
SmoI	CTYRAG	3 cut(s)	1883, 2364, 2523
SpeI	ACTAGT	1 cut(s)	956
SphI	GCATGC	3 cut(s)	154, 720, 1627
SseBI	AGGCCT	2 cut(s)	832, 1149
SspI	AATATT	3 cut(s)	586, 1494, 1597
SspMI	CTAG	1 cut(s)	957
SstI	GAGCTC	1 cut(s)	127
StuI	AGGCCT	2 cut(s)	832, 1149
StyD4I	CCNGG	8 cut(s)	51, 433, 697, 1547, 1779, 1872, 2100, 2598
StyI	CCWWGG	6 cut(s)	23, 668, 1237, 1262, 1314, 1486
TaaI	ACNGT	8 cut(s)	291, 1345, 1441, 1483, 1753, 2186, 2271, 2437
TaiI	ACGT	5 cut(s)	108, 877, 1122, 1299, 2678
TaqI	TCGA	3 cut(s)	135, 448, 1963
TaqII	GACCGA	1 cut(s)	1667
TatI	WGTACW	3 cut(s)	87, 291, 1302
TauI	GCSGC	2 cut(s)	150, 270
TfiI	GAWTC	3 cut(s)	168, 1076, 2314
TscAI	CASTG	6 cut(s)	285, 352, 1315, 1675, 1688, 1758
TseFI	GTSAC	4 cut(s)	425, 1510, 1679, 2648
TseI	GCWGC	9 cut(s)	18, 481, 559, 632, 1037, 1070, 1604, 1699, 2532
Tsp45I	GTSAC	4 cut(s)	425, 1510, 1679, 2648
TspDTI	ATGAA	7 cut(s)	588, 999, 1224, 1384, 1938, 2439, 2562
TspRI	CASTG	6 cut(s)	285, 352, 1315, 1675, 1688, 1758
Van91I	CCANNNNNTGG	2 cut(s)	1431, 2159
Vha464I	CTTAAG	1 cut(s)	1883
VneI	GTGCAC	1 cut(s)	1323
VpaK11BI	GGWCC	3 cut(s)	545, 1100, 1483
XapI	RAATTY	4 cut(s)	331, 751, 1132, 2501
XceI	RCATGY	5 cut(s)	154, 720, 1037, 1627, 2008
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1458, 2596
XmnI	GAANNNNTTC	1 cut(s)	2402
XspI	CTAG	1 cut(s)	957
ZrmI	AGTACT	1 cut(s)	89
Zsp2I	ATGCAT	3 cut(s)	259, 718, 805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.