pycom520g00430

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0005060
Physical Location & Seq
Forward (+)
355803 .. 357840
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGTGGAGGACGATGAGAATGAAATTGGGTGGTACAGGCGGCGGAAGAAGATGGTTGAGCAGTCGGAGTGATTGGGGGAAGGGGAAGAGGATCGCGGCGGTGTGGGGGAACGGGGACTACGGGAGGCTTGGGCTCGGTAGCCTGGACTCTCAGTGGAAGCCTGCCCCTTTGCGTTCGTCTGCCTTTGGCGACCAATCCCCTGAAGCCATAGCTTGCGGCGGCGCTCACACTCTCTTCTTAACGGGCAGCGGCCGCCTTTACGCCACCGGTCTCAATGATTTTGGACAGCTTGGAGTTGGGGATTCTCATGATTTAACTTACACTTGTAAGCCCTTAGAGGTAACTGGAATTGGCAAGGAAATTGTTCGAATTTCGGCCGGTTACTATCACTCTTGTGCTATTACAGAAGATGGAGAATTGTACATGTGGGGGAAGAACTCAAATGGGCAGCTTGGTCTTGGAAAAAGGGCTGCAGGGCTAGTTTCTGTACCAACCAAAGTTGAATGCTTGGCTGGAATCACCATTAAGACAGCAGCTTTGGGATCAGAGCACTCAGTAGCTGTTACTGATGGAGGTGAGTCCTTAAGTTGGGGAGGAGGGTCTGGGAGACTTGGTCATGGCCACGAATCAGGAATTTTAGGAATTCTCAGAAGTACCAGGTGGTATTTGATTTCTTTTATTACACGATTAAATAGAAATTTTTTTTCCCTTTGCTATGTTTCAACTGTGAAGTTCCCACTGCCTACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

251

Amino Acids

26.65

Weight (kDa)

9.46

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 34 - 207 7.4e-44 RCC1-like domain
RCC1 PF00415 35 - 80 3.2e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 69 - 96 1.3e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 83 - 134 2.6e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 121 - 150 2.9e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 137 - 188 3.2e-13 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 95
AciI CCGC 8 cut(s) 39, 42, 95, 98, 216, 219, 249, 253
AclWI GGATC 2 cut(s) 98, 550
AcoI YGGCCR 3 cut(s) 250, 375, 619
AcsI RAATTY 4 cut(s) 369, 633, 642, 697
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 4 cut(s) 34, 422, 489, 655
AfiI CCNNNNNNNGG 1 cut(s) 588
AflII CTTAAG 1 cut(s) 583
AflIII ACRYGT 1 cut(s) 423
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 2 cut(s) 503, 723
AjnI CCWGG 2 cut(s) 141, 656
AleI CACNNNNGTG 1 cut(s) 393
AluBI AGCT 5 cut(s) 212, 289, 451, 536, 560
AluI AGCT 5 cut(s) 212, 289, 451, 536, 560
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 2 cut(s) 275, 601
AlwI GGATC 2 cut(s) 98, 550
AlwNI CAGNNNCTG 1 cut(s) 560
AoxI GGCC 3 cut(s) 250, 375, 619
ApeKI GCWGC 4 cut(s) 246, 448, 470, 533
ApoI RAATTY 4 cut(s) 369, 633, 642, 697
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
AsiGI ACCGGT 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 511, 587
AsuII TTCGAA 1 cut(s) 367
BalI TGGCCA 1 cut(s) 621
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 4 cut(s) 258, 457, 460, 545
BccI CCATC 3 cut(s) 45, 404, 563
BciT130I CCWGG 2 cut(s) 143, 658
BcoDI GTCTC 2 cut(s) 275, 601
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 583
Bme1390I CCNGG 2 cut(s) 143, 658
BmrFI CCNGG 2 cut(s) 143, 658
Bpu14I TTCGAA 1 cut(s) 367
BsaI GGTCTC 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 2 cut(s) 266, 377
Bse1I ACTGG 1 cut(s) 349
BseBI CCWGG 2 cut(s) 143, 658
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 164, 567, 661
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 1 cut(s) 609
BseX3I CGGCCG 2 cut(s) 250, 375
BseXI GCAGC 4 cut(s) 258, 457, 460, 545
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 2 cut(s) 253, 378
BshFI GGCC 3 cut(s) 252, 377, 621
BshTI ACCGGT 1 cut(s) 266
BsiEI CGRYCG 2 cut(s) 253, 378
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 2 cut(s) 267, 378
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 2 cut(s) 275, 601
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 3 cut(s) 252, 377, 621
Bso31I GGTCTC 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 135, 552
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 90, 542
BspACI CCGC 8 cut(s) 39, 42, 95, 98, 216, 219, 249, 253
BspANI GGCC 3 cut(s) 252, 377, 621
BspCNI CTCAG 3 cut(s) 163, 566, 660
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 307
BspMAI CTGCAG 1 cut(s) 475
BspPI GGATC 2 cut(s) 98, 550
BspT104I TTCGAA 1 cut(s) 367
BspTI CTTAAG 1 cut(s) 583
BspTNI GGTCTC 1 cut(s) 275
BsrFI RCCGGY 2 cut(s) 266, 377
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 2 cut(s) 266, 377
BssMI GATC 2 cut(s) 90, 542
Bst2UI CCWGG 2 cut(s) 143, 658
Bst4CI ACNGT 1 cut(s) 727
Bst6I CTCTTC 2 cut(s) 80, 239
BstAFI CTTAAG 1 cut(s) 583
BstAUI TGTACA 1 cut(s) 420
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 162, 214
BstDEI CTNAG 4 cut(s) 150, 334, 553, 647
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 2 cut(s) 93, 545
BstMAI GTCTC 2 cut(s) 275, 601
BstMBI GATC 2 cut(s) 90, 542
BstMCI CGRYCG 2 cut(s) 253, 378
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 2 cut(s) 143, 658
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 2 cut(s) 141, 656
BstSFI CTRYAG 1 cut(s) 471
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 258, 457, 460, 545
BstZI CGGCCG 2 cut(s) 250, 375
BsuRI GGCC 3 cut(s) 252, 377, 621
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 2 cut(s) 158, 737
Cac8I GCNNGC 2 cut(s) 162, 214
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 307
CciNI GCGGCCGC 1 cut(s) 250
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 2 cut(s) 266, 377
CsiI ACCWGGT 1 cut(s) 656
Csp6I GTAC 4 cut(s) 33, 421, 488, 654
CspAI ACCGGT 1 cut(s) 266
CviAII CATG 3 cut(s) 308, 424, 617
CviQI GTAC 4 cut(s) 33, 421, 488, 654
DdeI CTNAG 4 cut(s) 150, 334, 553, 647
DpnI GATC 2 cut(s) 92, 544
DpnII GATC 2 cut(s) 90, 542
EaeI YGGCCR 3 cut(s) 250, 375, 619
EagI CGGCCG 2 cut(s) 250, 375
Eam1104I CTCTTC 2 cut(s) 80, 239
EarI CTCTTC 2 cut(s) 80, 239
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 2 cut(s) 250, 375
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 275
Eco52I CGGCCG 2 cut(s) 250, 375
Eco57I CTGAAG 1 cut(s) 222
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 2 cut(s) 141, 656
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 3 cut(s) 311, 427, 620
FaiI YATR 5 cut(s) 209, 309, 425, 618, 717
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 307, 423, 616
FriOI GRGCYC 1 cut(s) 135
FspBI CTAG 1 cut(s) 479
GlaI GCGC 1 cut(s) 223
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 252, 377, 621
HapII CCGG 2 cut(s) 267, 378
HhaI GCGC 1 cut(s) 224
Hin1II CATG 3 cut(s) 311, 427, 620
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HinfI GANTC 5 cut(s) 146, 302, 516, 578, 626
HpaII CCGG 2 cut(s) 267, 378
HphI GGTGA 2 cut(s) 511, 587
Hpy188I TCNGA 4 cut(s) 66, 547, 650, 752
Hpy188III TCNNGA 2 cut(s) 308, 630
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 1 cut(s) 727
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 4 cut(s) 150, 334, 553, 647
Hsp92II CATG 3 cut(s) 311, 427, 620
HspAI GCGC 1 cut(s) 222
Kzo9I GATC 2 cut(s) 90, 542
Lsp1109I GCAGC 4 cut(s) 258, 457, 460, 545
MabI ACCWGGT 1 cut(s) 656
MaeI CTAG 1 cut(s) 479
MaeIII GTNAC 3 cut(s) 340, 380, 562
MalI GATC 2 cut(s) 92, 544
MboI GATC 2 cut(s) 90, 542
MboII GAAGA 6 cut(s) 57, 60, 97, 226, 419, 445
MhlI GDGCHC 2 cut(s) 135, 552
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 8 cut(s) 23, 348, 360, 369, 416, 633, 642, 697
MluNI TGGCCA 1 cut(s) 621
MlyI GAGTC 2 cut(s) 140, 587
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 6 cut(s) 81, 117, 331, 566, 587, 590
Mox20I TGGCCA 1 cut(s) 621
MroXI GAANNNNTTC 1 cut(s) 363
MscI TGGCCA 1 cut(s) 621
MseI TTAA 5 cut(s) 239, 314, 525, 584, 689
MslI CAYNNNNRTG 1 cut(s) 393
Msp20I TGGCCA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 2 cut(s) 267, 378
MspR9I CCNGG 2 cut(s) 143, 658
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 2 cut(s) 143, 658
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 2 cut(s) 90, 542
NlaIII CATG 3 cut(s) 311, 427, 620
NotI GCGGCCGC 1 cut(s) 250
NspI RCATGY 1 cut(s) 427
NspV TTCGAA 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 393
PagI TCATGA 1 cut(s) 307
PciI ACATGT 1 cut(s) 423
PctI GAATGC 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 3 cut(s) 302, 516, 626
PflFI GACNNNGTC 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 266
PleI GAGTC 2 cut(s) 140, 586
PpsI GAGTC 2 cut(s) 140, 586
PscI ACATGT 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 141, 656
PspGI CCWGG 2 cut(s) 141, 656
PsrI GAACNNNNNNTAC 2 cut(s) 101, 133
PstI CTGCAG 1 cut(s) 475
PstNI CAGNNNCTG 1 cut(s) 560
PsyI GACNNNGTC 1 cut(s) 612
RsaI GTAC 4 cut(s) 34, 422, 489, 655
RsaNI GTAC 4 cut(s) 33, 421, 488, 654
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 5 cut(s) 239, 314, 525, 584, 689
Sau3AI GATC 2 cut(s) 90, 542
SchI GAGTC 2 cut(s) 140, 587
ScrFI CCNGG 2 cut(s) 143, 658
SduI GDGCHC 2 cut(s) 135, 552
SetI ASST 8 cut(s) 214, 291, 342, 453, 538, 562, 577, 662
SexAI ACCWGGT 1 cut(s) 656
SfcI CTRYAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 8 cut(s) 23, 348, 360, 369, 416, 633, 642, 697
SsiI CCGC 8 cut(s) 39, 42, 95, 98, 216, 219, 249, 253
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 141, 656
TaaI ACNGT 1 cut(s) 727
TaqI TCGA 1 cut(s) 367
TasI AATT 8 cut(s) 23, 348, 360, 369, 416, 633, 642, 697
TatI WGTACW 1 cut(s) 420
TauI GCSGC 6 cut(s) 42, 98, 219, 222, 252, 255
TfiI GAWTC 3 cut(s) 302, 516, 626
Tru1I TTAA 5 cut(s) 239, 314, 525, 584, 689
Tru9I TTAA 5 cut(s) 239, 314, 525, 584, 689
TscAI CASTG 2 cut(s) 158, 744
TseI GCWGC 4 cut(s) 246, 448, 470, 533
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 2 cut(s) 158, 744
Tth111I GACNNNGTC 1 cut(s) 612
Vha464I CTTAAG 1 cut(s) 583
XapI RAATTY 4 cut(s) 369, 633, 642, 697
XceI RCATGY 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.