pycom520g00440

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0005060
Physical Location & Seq
Forward (+)
360537 .. 362209
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGATCAGCAAACTGCCATGCTCCGAAGAAGTTGCTTGTGGTGGCTATCACACTTGTGTCTTAACAAGTGGTGGAGAACTGTACAGTTGGGGATCAAATGAAAATGGGTGCCTTGGCATTGGTACGACAGATGTTTTTCACCACCCAGAAAGAGTTCAGGGACCTTTTTTAAAATCCCCGATTGTCAAGGTATCGTGCGGTTGGAAGCATACAGCTGCAATTTCTGAAGGCAATATTTATACATGGGGTTGGGGAGGTTCCCTTGGAACATTTTCCGAAGACGGACATTCTTCTGGAGGACAACTGGGTCATGGAACTGATGTTGACTACATAAAACCTACAATGGTTACGTTTGGGCAGAATGTGAAAGCACTACAAATATCATGTGGATTCAATCACACTGGTGCCATAGTTGAATACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
RCC1

Protein Analysis

142

Amino Acids

14.96

Weight (kDa)

5.71

Isoelectric Point (pI)

27.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 9 - 134 9.4e-29 RCC1-like domain
RCC1_2 PF13540 11 - 37 6.7e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 25 - 74 3.6e-16 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 96 - 137 1.1e-07 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 306
AccB1I GGYRCC 2 cut(s) 108, 404
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 2 cut(s) 83, 124
AgsI TTSAA 2 cut(s) 394, 416
AleI CACNNNNGTG 2 cut(s) 54, 402
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
AlwI GGATC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 215
Asp700I GAANNNNTTC 2 cut(s) 153, 271
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 131
AvaII GGWCC 1 cut(s) 161
BanI GGYRCC 2 cut(s) 108, 404
BbsI GAAGAC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BclI TGATCA 1 cut(s) 3
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 4 cut(s) 110, 162, 259, 406
BmrI ACTGGG 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 112, 262
Bse1I ACTGG 2 cut(s) 309, 406
BseDI CCNNGG 2 cut(s) 112, 262
BseNI ACTGG 2 cut(s) 309, 406
BseXI GCAGC 1 cut(s) 202
BshNI GGYRCC 2 cut(s) 108, 404
BslFI GGGAC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 2 cut(s) 3, 92
BspACI CCGC 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 110, 162, 259, 406
BspPI GGATC 1 cut(s) 100
BspT107I GGYRCC 2 cut(s) 108, 404
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 2 cut(s) 309, 406
BssECI CCNNGG 2 cut(s) 112, 262
BssMI GATC 2 cut(s) 3, 92
BssT1I CCWWGG 2 cut(s) 112, 262
Bst4CI ACNGT 2 cut(s) 81, 86
BstAUI TGTACA 1 cut(s) 81
BstKTI GATC 2 cut(s) 6, 95
BstMBI GATC 2 cut(s) 3, 92
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 2 cut(s) 82, 123
CviAII CATG 4 cut(s) 18, 243, 311, 384
CviJI RGCY 2 cut(s) 45, 215
CviKI_1 RGCY 2 cut(s) 45, 215
CviQI GTAC 2 cut(s) 82, 123
DpnI GATC 2 cut(s) 5, 94
DpnII GATC 2 cut(s) 3, 92
DraI TTTAAA 1 cut(s) 171
DrdI GACNNNNNNGTC 1 cut(s) 306
DseDI GACNNNNNNGTC 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 112, 262
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 112, 262
ErhI CCWWGG 2 cut(s) 112, 262
FaeI CATG 4 cut(s) 21, 246, 314, 387
FaiI YATR 9 cut(s) 19, 210, 240, 244, 312, 332, 385, 410, 424
FaqI GGGAC 1 cut(s) 174
FatI CATG 4 cut(s) 17, 242, 310, 383
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 315
Hin1II CATG 4 cut(s) 21, 246, 314, 387
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 1 cut(s) 390
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 3 cut(s) 25, 226, 277
Hpy188III TCNNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 2 cut(s) 81, 86
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 4 cut(s) 21, 246, 314, 387
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 92
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 5 cut(s) 143, 159, 279, 290, 387
Lsp1109I GCAGC 1 cut(s) 202
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 5, 94
MboI GATC 2 cut(s) 3, 92
MboII GAAGA 3 cut(s) 38, 282, 290
MluCI AATT 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 2 cut(s) 248, 290
MroXI GAANNNNTTC 2 cut(s) 153, 271
MseI TTAA 2 cut(s) 62, 170
MslI CAYNNNNRTG 2 cut(s) 54, 402
MspA1I CMGCKG 1 cut(s) 215
NdeII GATC 2 cut(s) 3, 92
NlaIII CATG 4 cut(s) 21, 246, 314, 387
NlaIV GGNNCC 4 cut(s) 110, 162, 259, 406
OliI CACNNNNGTG 2 cut(s) 54, 402
PdmI GAANNNNTTC 2 cut(s) 153, 271
PfeI GAWTC 1 cut(s) 390
PkrI GCNGC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 161
Psp5II RGGWCCY 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 110, 162, 259, 406
PspPI GGNCC 1 cut(s) 161
PspPPI RGGWCCY 1 cut(s) 161
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 2 cut(s) 83, 124
RsaNI GTAC 2 cut(s) 82, 123
RseI CAYNNNNRTG 2 cut(s) 54, 402
SaqAI TTAA 2 cut(s) 62, 170
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 3, 92
Sau96I GGNCC 1 cut(s) 161
SetI ASST 6 cut(s) 166, 192, 217, 259, 340, 353
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 54, 402
Sse9I AATT 1 cut(s) 219
SsiI CCGC 1 cut(s) 198
SspI AATATT 1 cut(s) 235
StyI CCWWGG 2 cut(s) 112, 262
TaaI ACNGT 2 cut(s) 81, 86
TaiI ACGT 1 cut(s) 353
TasI AATT 1 cut(s) 219
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 2 cut(s) 62, 170
Tru9I TTAA 2 cut(s) 62, 170
TscAI CASTG 1 cut(s) 406
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 161
XmnI GAANNNNTTC 2 cut(s) 153, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.