RchiOBHm_Chr7g0188051

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008204
Physical Location & Seq
Forward (+)
7722741 .. 7726254
3514 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGGCAGATTCCGAGGACATTCAGCCTCTTGTCTGTGATAATGGTACTGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCCCCAAGGGCTGTCTTCCCCAGTATTGTTGGTCGACCTAGGCACACAGGTGTTATGGTGGGGATGGGACAGAAAGACGCATATGTTGGGGATGAGGCTCAGTCCAAAAGAGGTATTCTCACTTTAAAATACCCTATTGAACATGGGATTGTTAGCAATTGGGACGACATGGAAAAGATCTGGCATCATACTTTCTACAATGAGCTTCGTGTGGCTCCTGAAGAACATCCTGTGCTCCTTACTGAGGCACCCCTCAATCCTAAAGCCAACAGAGAGAAGATGACACAGATCATGTTTGAGACCTTCAATTCCCCTGCTATGTATGTTGCCATTCAAGCAGTTCTCTCCCTCTATGCCAGTGGGCGTACAACAGGTATTGTGCTCGACTCTGGTGATGGAGTCAGTCACACAGTTCCCATCTACGAAGGTTATGCACTTCCACATGCCATTCTGCGGCTTGACCTTGCTGGACGAGATTTAACAGACTCCTTAATGAAGATCCTGACTGAGAGAGGGTATTCTTTTACTACAACTGCTGAACGGGAAATTGTACGTGACATTAAAGAGAAGCTTGCATATGTGGCACTTGATTATGAACAAGAACTTGAGACTGCTGCAAGCAGCTCCACAATTGAGAAGAACTATGAACTACCTGATGGTCAGGTAATTACCATTGGAGCAGAGCGTTTCCGTTGCCCTGAAGTCCTATTTCAACCATCACTAGTTGGAATGGAGGCAGCTGGAATTCACGAGACCACCTATAACTCGATTATGAAGTGTGATGTTGACATCAGGAAAGACTTGTATGGTAATATTGTGCTCAGTGGTGGGTCTACTATGTTCCCAGGAATCGCTGATAGGATGAGCAAGGAGATAACAGCCCTTGCACCAAGCAGTATGAAGATCAAGGTGGTGGCACCTCCTGAGAGAAAGTACAGTGTTTGGATCGGAGGATCCATATTGGCTTCACTAAGTACATTCCAGCAGATGTGGATATCTAAGGCAGAGTACGATGAATCCGGCCCATCCATCGTCCACAGAAAATGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

377

Amino Acids

41.62

Weight (kDa)

5.23

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 7.3e-147 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 328, 997
AccI GTMKAC 2 cut(s) 115, 914
AciI CCGC 1 cut(s) 535
AclWI GGATC 4 cut(s) 574, 1029, 1034, 1042
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 2 cut(s) 321, 801
AfaI GTAC 6 cut(s) 46, 448, 633, 1016, 1057, 1091
AfiI CCNNNNNNNGG 2 cut(s) 325, 534
AgsI TTSAA 4 cut(s) 221, 388, 416, 794
AhlI ACTAGT 1 cut(s) 802
AjnI CCWGG 1 cut(s) 925
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 4 cut(s) 286, 652, 705, 821
AluI AGCT 4 cut(s) 286, 652, 705, 821
Alw21I GWGCWC 3 cut(s) 318, 465, 903
Alw26I GTCTC 3 cut(s) 374, 683, 827
AlwI GGATC 4 cut(s) 574, 1029, 1034, 1042
AoxI GGCC 1 cut(s) 1102
ApeKI GCWGC 3 cut(s) 695, 702, 818
ApoI RAATTY 1 cut(s) 825
AspA2I CCTAGG 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 1103
AsuHPI GGTGA 1 cut(s) 485
AvrII CCTAGG 1 cut(s) 119
BamHI GGATCC 1 cut(s) 1034
BanI GGYRCC 2 cut(s) 328, 997
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 3 cut(s) 318, 465, 903
BbvI GCAGC 3 cut(s) 682, 714, 830
BccI CCATC 7 cut(s) 139, 470, 506, 731, 805, 1114, 1118
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciT130I CCWGG 1 cut(s) 927
BcoDI GTCTC 3 cut(s) 374, 683, 827
BcuI ACTAGT 1 cut(s) 802
BfaI CTAG 3 cut(s) 120, 803, 1132
BglI GCCNNNNNGGC 1 cut(s) 89
BglII AGATCT 1 cut(s) 258
BisI GCNGC 4 cut(s) 536, 696, 703, 819
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 4 cut(s) 537, 697, 704, 820
Bme1390I CCNGG 1 cut(s) 927
BmgT120I GGNCC 1 cut(s) 1103
BmiI GGNNCC 4 cut(s) 297, 330, 999, 1036
BmrFI CCNGG 1 cut(s) 927
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 2 cut(s) 70, 274
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 707
BsaAI YACGTR 1 cut(s) 635
BsaI GGTCTC 2 cut(s) 374, 827
BsaJI CCNNGG 4 cut(s) 12, 86, 119, 925
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 534
Bse1I ACTGG 3 cut(s) 52, 102, 438
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 12, 86, 119, 925
BseGI GGATG 5 cut(s) 150, 178, 307, 948, 1106
BseLI CCNNNNNNNGG 2 cut(s) 325, 534
BseMII CTCAG 5 cut(s) 194, 315, 579, 916, 996
BseNI ACTGG 3 cut(s) 52, 102, 438
BseXI GCAGC 3 cut(s) 682, 714, 830
BshFI GGCC 1 cut(s) 1104
BshNI GGYRCC 2 cut(s) 328, 997
BsiHKAI GWGCWC 3 cut(s) 318, 465, 903
BsiSI CCGG 1 cut(s) 1101
BslFI GGGAC 2 cut(s) 162, 257
BslI CCNNNNNNNGG 2 cut(s) 325, 534
BsmAI GTCTC 3 cut(s) 374, 683, 827
BsmFI GGGAC 2 cut(s) 162, 257
BsnI GGCC 1 cut(s) 1104
Bso31I GGTCTC 2 cut(s) 374, 827
Bsp1286I GDGCHC 3 cut(s) 318, 465, 903
Bsp143I GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
BspACI CCGC 1 cut(s) 535
BspANI GGCC 1 cut(s) 1104
BspCNI CTCAG 5 cut(s) 193, 316, 580, 915, 997
BspLI GGNNCC 4 cut(s) 297, 330, 999, 1036
BspPI GGATC 4 cut(s) 574, 1029, 1034, 1042
BspT107I GGYRCC 2 cut(s) 328, 997
BspTNI GGTCTC 2 cut(s) 374, 827
BsrI ACTGG 3 cut(s) 52, 102, 438
BssECI CCNNGG 4 cut(s) 12, 86, 119, 925
BssMI GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 2 cut(s) 86, 119
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 2 cut(s) 493, 1019
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 654, 700
BstDEI CTNAG 7 cut(s) 180, 324, 588, 902, 1005, 1052, 1080
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 5 cut(s) 150, 178, 307, 948, 1106
BstKTI GATC 6 cut(s) 261, 372, 582, 987, 1029, 1037
BstMAI GTCTC 3 cut(s) 374, 683, 827
BstMBI GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 1 cut(s) 927
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 925
BstV1I GCAGC 3 cut(s) 682, 714, 830
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 3 cut(s) 258, 579, 1034
BstYI RGATCY 3 cut(s) 258, 579, 1034
BsuRI GGCC 1 cut(s) 1104
BtsCI GGATG 5 cut(s) 150, 178, 307, 948, 1106
BtsIMutI CAGTG 3 cut(s) 445, 910, 1024
Cac8I GCNNGC 2 cut(s) 654, 700
Cfr13I GGNCC 1 cut(s) 1103
CseI GACGC 1 cut(s) 167
Csp6I GTAC 6 cut(s) 45, 447, 632, 1015, 1056, 1090
CviAII CATG 4 cut(s) 224, 250, 373, 524
CviQI GTAC 6 cut(s) 45, 447, 632, 1015, 1056, 1090
DdeI CTNAG 7 cut(s) 180, 324, 588, 902, 1005, 1052, 1080
DpnI GATC 6 cut(s) 260, 371, 581, 986, 1028, 1036
DpnII GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
DraI TTTAAA 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 86, 119
Eco31I GGTCTC 2 cut(s) 374, 827
Eco32I GATATC 1 cut(s) 1077
Eco57I CTGAAG 2 cut(s) 321, 801
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 825
EcoRII CCWGG 1 cut(s) 925
EcoRV GATATC 1 cut(s) 1077
EcoT14I CCWWGG 2 cut(s) 86, 119
ErhI CCWWGG 2 cut(s) 86, 119
FaeI CATG 4 cut(s) 227, 253, 376, 527
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 2 cut(s) 162, 257
FatI CATG 4 cut(s) 223, 249, 372, 523
FauNDI CATATG 2 cut(s) 163, 658
FblI GTMKAC 2 cut(s) 115, 914
Fnu4HI GCNGC 4 cut(s) 536, 696, 703, 819
FokI GGATG 5 cut(s) 157, 185, 294, 955, 1093
Fsp4HI GCNGC 4 cut(s) 536, 696, 703, 819
FspBI CTAG 3 cut(s) 120, 803, 1132
GluI GCNGC 4 cut(s) 536, 696, 703, 819
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 1 cut(s) 1101
HgaI GACGC 1 cut(s) 167
Hin1II CATG 4 cut(s) 227, 253, 376, 527
HincII GTYRAC 2 cut(s) 116, 868
HindII GTYRAC 2 cut(s) 116, 868
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 6 cut(s) 8, 467, 480, 566, 930, 1097
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 4 cut(s) 116, 868, 915, 1117
Hpy188I TCNGA 2 cut(s) 13, 1031
Hpy188III TCNNGA 5 cut(s) 299, 583, 830, 874, 1004
Hpy8I GTNNAC 4 cut(s) 116, 868, 915, 1117
HpyAV CCTTC 2 cut(s) 394, 500
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4IV ACGT 1 cut(s) 634
HpyCH4V TGCA 4 cut(s) 515, 656, 698, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 7 cut(s) 180, 324, 588, 902, 1005, 1052, 1080
HpySE526I ACGT 1 cut(s) 634
Hsp92II CATG 4 cut(s) 227, 253, 376, 527
Kzo9I GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
LmnI GCTCC 4 cut(s) 301, 321, 710, 758
Lsp1109I GCAGC 3 cut(s) 682, 714, 830
LweI GCATC 2 cut(s) 70, 274
MaeI CTAG 3 cut(s) 120, 803, 1132
MaeII ACGT 1 cut(s) 634
MaeIII GTNAC 2 cut(s) 485, 635
MalI GATC 6 cut(s) 260, 371, 581, 986, 1028, 1036
MboI GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
MboII GAAGA 6 cut(s) 88, 314, 370, 589, 730, 994
MfeI CAATTG 2 cut(s) 238, 711
MflI RGATCY 3 cut(s) 258, 579, 1034
MhlI GDGCHC 3 cut(s) 318, 465, 903
MluCI AATT 6 cut(s) 238, 388, 627, 711, 747, 825
MlyI GAGTC 3 cut(s) 461, 489, 560
MmeI TCCRAC 1 cut(s) 787
MseI TTAA 5 cut(s) 57, 206, 560, 572, 642
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 1 cut(s) 927
MunI CAATTG 2 cut(s) 238, 711
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NdeI CATATG 2 cut(s) 163, 658
NdeII GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
NlaIII CATG 4 cut(s) 227, 253, 376, 527
NlaIV GGNNCC 4 cut(s) 297, 330, 999, 1036
NmuCI GTSAC 2 cut(s) 485, 635
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 1 cut(s) 129
PfeI GAWTC 3 cut(s) 8, 930, 1097
PkrI GCNGC 4 cut(s) 537, 697, 704, 820
PleI GAGTC 3 cut(s) 461, 488, 560
PpsI GAGTC 3 cut(s) 461, 488, 560
Ppu21I YACGTR 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 4 cut(s) 297, 330, 999, 1036
PspPI GGNCC 1 cut(s) 1103
PsuI RGATCY 3 cut(s) 258, 579, 1034
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 6 cut(s) 46, 448, 633, 1016, 1057, 1091
RsaNI GTAC 6 cut(s) 45, 447, 632, 1015, 1056, 1090
RseI CAYNNNNRTG 1 cut(s) 129
SalI GTCGAC 1 cut(s) 114
SaqAI TTAA 5 cut(s) 57, 206, 560, 572, 642
SatI GCNGC 4 cut(s) 536, 696, 703, 819
Sau3AI GATC 6 cut(s) 258, 369, 579, 984, 1026, 1034
Sau96I GGNCC 1 cut(s) 1103
SchI GAGTC 3 cut(s) 461, 489, 560
ScrFI CCNGG 1 cut(s) 927
SduI GDGCHC 3 cut(s) 318, 465, 903
SfaNI GCATC 2 cut(s) 70, 274
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
SpeI ACTAGT 1 cut(s) 802
Sse9I AATT 6 cut(s) 238, 388, 627, 711, 747, 825
SsiI CCGC 1 cut(s) 535
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 120, 803, 1132
StyD4I CCNGG 1 cut(s) 925
StyI CCWWGG 2 cut(s) 86, 119
TaaI ACNGT 2 cut(s) 493, 1019
TaiI ACGT 1 cut(s) 637
TaqI TCGA 3 cut(s) 115, 465, 848
TasI AATT 6 cut(s) 238, 388, 627, 711, 747, 825
TatI WGTACW 2 cut(s) 1014, 1055
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 3 cut(s) 8, 930, 1097
Tru1I TTAA 5 cut(s) 57, 206, 560, 572, 642
Tru9I TTAA 5 cut(s) 57, 206, 560, 572, 642
TscAI CASTG 3 cut(s) 445, 910, 1024
TseFI GTSAC 2 cut(s) 485, 635
TseI GCWGC 3 cut(s) 695, 702, 818
Tsp45I GTSAC 2 cut(s) 485, 635
TspDTI ATGAA 6 cut(s) 590, 690, 741, 869, 995, 1110
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 3 cut(s) 445, 910, 1024
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 825
XceI RCATGY 1 cut(s) 527
XmaJI CCTAGG 1 cut(s) 119
XmiI GTMKAC 2 cut(s) 115, 914
XspI CTAG 3 cut(s) 120, 803, 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.