RchiOBHm_Chr7g0232091

Protein phosphatase methylesterase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012939
Physical Location & Seq
Reverse (-)
56028914 .. 56036474
7561 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ATGGATTCTTCAAACCTCACTTCCCTTCCCGAGGACACCCCCGAATCCAATCAACCCCCCCACCACCACCAACAGCCGCCACCCCAACCCCGTCCTCTCTCCGCCTTCGCCGCCCGCCCCGATCGCCCAGCCACTCAGAGTAGCTCGCAAAAGTTCGCCGCTTTAGATTGGAAGGACTATTTCGACAAAGAAGATGATATCTCCGTTCCGGGCTCCGACGATGTTTTTCATGTATATATGGCAGGAACAGAGGGTCCGGTTGTGTTTTGCCTACATGGAGGTGGCTACTCAGGGCTTTCATTTGCCTTGTCAGCAAGGCAAATCAAGGGGAAGGCTCGAGTAGTTGCCATGGACTTGAGAGGACATGGAAGGTCGACGAATGAAAATGAGACTGATCTTTCTATTGAGACCATGTGCAATGATGTTGAGGCTGTTCTGAAAGCAATGTATGGAGATTCTCCTCCTGCAATTGTGCTTGTTGGCCACAGTATGGGAGGGTCAGTAGCTGTGCATGTTGCTGCAAGAAAAGCATTGCCTAGCTTGGCTGGGCTCGTTGTTGTAGATGTTGTTGAGGGAACAGCAATGGCTTCGTTGATTCATATGCAAAAAATCCTATCAGGCAGGACGCATCATTTTTCAAGCATTGAAAAAGCGATAGAATCGACTGTAAAGGGAGGCTCTTTGAGAAACATTGAGTCTGCTCGTGTATCAGTACCTGCCACATTAAAGTATGATGATTCAAAGAAATGTTACATTTACAGAGCACGACTCGAAGAAACAGAACAATATTGGAAAAGCTGGTATGAAGGCCTTTCTGAAAAATTTCTTTCATCTCCTGTTCCAAAGCTCCTGCTTTTAGCTGGAACAGACCGACTCGACAGAACTCTGACAATTGGTCAAATGCAAGGCAAGTTCCAAATGGTGGTTGTCAGACATACTGGGCATGCCATACAAGAAGATGTACCTGATGAATTTGCAACGCTAATAATAAATTTCATATCCCGTAACCGTATAGGCCCACATGGAGTTGAGATTCCAGGACTTCGTCGGCCATCCCAGCCCAAACCTTGA

Protein Analysis

356

Amino Acids

38.96

Weight (kDa)

6.54

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 86 - 320 7.5e-19 alpha/beta hydrolase fold
Hydrolase_4 PF12146 87 - 187 6.1e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 88 - 327 1.4e-26 Alpha/beta hydrolase family
Thioesterase PF00975 88 - 175 1.2e-07 Thioesterase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10050 AT4G10050
fragaria_vesca FvH4_5g33360 FvH4_5g33360
malus_domestica MD08G1213000.v1.1 MD15G1395500.v1.1
prunus_persica Prupe.1G544300_v2.0.a1 Prupe.1G544300_v2.0.a1 Prupe.1G544300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0232091
rosa_laevigata RLG00000001392
rosa_multiflora Rmu_sc0000616.1_g000007 Rmu_sc0004618.1_g000006
rosa_roxburghii Rroxscaffold_3G00228820
rosa_rugosa Rorug07G0269000
rosa_samantha Rh7BG397600 Rh7CG443900
rosa_wichuraiana Rw7G035030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 724
AccB7I CCANNNNNTGG 2 cut(s) 490, 922
AccI GTMKAC 1 cut(s) 374
AciI CCGC 5 cut(s) 77, 102, 111, 115, 159
AcoI YGGCCR 2 cut(s) 481, 1049
AcsI RAATTY 3 cut(s) 821, 971, 991
AfaI GTAC 2 cut(s) 714, 963
AfiI CCNNNNNNNGG 3 cut(s) 31, 490, 922
AgsI TTSAA 4 cut(s) 12, 639, 647, 741
AhdI GACNNNNNGTC 1 cut(s) 894
AjnI CCWGG 1 cut(s) 1036
AloI GAACNNNNNNTCC 2 cut(s) 238, 270
AluBI AGCT 6 cut(s) 144, 506, 540, 798, 847, 860
AluI AGCT 6 cut(s) 144, 506, 540, 798, 847, 860
Alw21I GWGCWC 1 cut(s) 766
Alw26I GTCTC 2 cut(s) 383, 401
AlwNI CAGNNNCTG 2 cut(s) 506, 716
Ama87I CYCGRG 2 cut(s) 29, 336
AoxI GGCC 4 cut(s) 481, 808, 1015, 1049
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 3 cut(s) 821, 971, 991
Asp700I GAANNNNTTC 1 cut(s) 822
AspS9I GGNCC 2 cut(s) 254, 1016
AsuC2I CCSGG 1 cut(s) 210
AvaI CYCGRG 2 cut(s) 29, 336
AvaII GGWCC 1 cut(s) 254
BalI TGGCCA 1 cut(s) 483
BanII GRGCYC 2 cut(s) 215, 552
BauI CACGAG 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 1 cut(s) 505
BccI CCATC 1 cut(s) 1060
BcgI CGANNNNNNTGC 2 cut(s) 570, 604
BciT130I CCWGG 1 cut(s) 1038
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 2 cut(s) 383, 401
BfaI CTAG 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 724
BisI GCNGC 4 cut(s) 77, 111, 159, 519
BlsI GCNGC 4 cut(s) 78, 112, 160, 520
Bme1390I CCNGG 2 cut(s) 210, 1038
Bme18I GGWCC 1 cut(s) 254
BmeRI GACNNNNNGTC 1 cut(s) 894
BmeT110I CYCGRG 2 cut(s) 29, 336
BmgT120I GGNCC 2 cut(s) 254, 1016
BmiI GGNNCC 2 cut(s) 214, 255
BmrFI CCNGG 2 cut(s) 210, 1038
BmrI ACTGGG 1 cut(s) 948
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 948
BplI GAGNNNNNCTC 2 cut(s) 753, 785
BpuEI CTTGAG 1 cut(s) 376
BpuMI CCSGG 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 30, 348
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 490, 922
Bse1I ACTGG 1 cut(s) 943
Bse3DI GCAATG 4 cut(s) 424, 450, 530, 588
BseBI CCWGG 1 cut(s) 1038
BseDI CCNNGG 2 cut(s) 30, 348
BseGI GGATG 1 cut(s) 1052
BseLI CCNNNNNNNGG 3 cut(s) 31, 490, 922
BseMI GCAATG 4 cut(s) 424, 450, 530, 588
BseMII CTCAG 2 cut(s) 149, 303
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 1 cut(s) 505
BseYI CCCAGC 3 cut(s) 127, 545, 1056
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 4 cut(s) 483, 810, 1017, 1051
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 766
BsiHKCI CYCGRG 2 cut(s) 29, 336
BsiSI CCGG 2 cut(s) 209, 257
BslI CCNNNNNNNGG 3 cut(s) 31, 490, 922
BsmAI GTCTC 2 cut(s) 383, 401
BsnI GGCC 4 cut(s) 483, 810, 1017, 1051
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 2 cut(s) 29, 336
Bsp1286I GDGCHC 3 cut(s) 215, 552, 766
Bsp143I GATC 2 cut(s) 121, 394
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 5 cut(s) 77, 102, 111, 115, 159
BspANI GGCC 4 cut(s) 483, 810, 1017, 1051
BspCNI CTCAG 2 cut(s) 148, 302
BspLI GGNNCC 2 cut(s) 214, 255
BspMI ACCTGC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 4 cut(s) 424, 450, 530, 588
BsrI ACTGG 1 cut(s) 943
BssECI CCNNGG 2 cut(s) 30, 348
BssMI GATC 2 cut(s) 121, 394
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 1038
Bst4CI ACNGT 3 cut(s) 488, 667, 1010
BstC8I GCNNGC 3 cut(s) 115, 146, 945
BstDEI CTNAG 2 cut(s) 135, 289
BstDSI CCRYGG 1 cut(s) 348
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 1 cut(s) 1052
BstKTI GATC 2 cut(s) 124, 397
BstMAI GTCTC 2 cut(s) 383, 401
BstMBI GATC 2 cut(s) 121, 394
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 5 cut(s) 110, 123, 311, 527, 1057
BstNI CCWGG 1 cut(s) 1038
BstNSI RCATGY 2 cut(s) 515, 947
BstSCI CCNGG 2 cut(s) 208, 1036
BstV1I GCAGC 1 cut(s) 505
BsuRI GGCC 4 cut(s) 483, 810, 1017, 1051
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 1052
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 3 cut(s) 115, 146, 945
CaiI CAGNNNCTG 2 cut(s) 506, 716
Cfr13I GGNCC 2 cut(s) 254, 1016
CseI GACGC 1 cut(s) 634
Csp6I GTAC 2 cut(s) 713, 962
CviAII CATG 8 cut(s) 230, 275, 349, 365, 412, 512, 944, 1022
CviQI GTAC 2 cut(s) 713, 962
DdeI CTNAG 2 cut(s) 135, 289
DpnI GATC 2 cut(s) 123, 396
DpnII GATC 2 cut(s) 121, 394
DriI GACNNNNNGTC 1 cut(s) 894
EaeI YGGCCR 2 cut(s) 481, 1049
Eam1105I GACNNNNNGTC 1 cut(s) 894
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 2 cut(s) 215, 552
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 254
Eco88I CYCGRG 2 cut(s) 29, 336
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 1036
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 2 cut(s) 215, 552
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 8 cut(s) 233, 278, 352, 368, 415, 515, 947, 1025
FatI CATG 8 cut(s) 229, 274, 348, 364, 411, 511, 943, 1021
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 1 cut(s) 600
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 4 cut(s) 77, 111, 159, 519
FokI GGATG 1 cut(s) 1039
FriOI GRGCYC 2 cut(s) 215, 552
Fsp4HI GCNGC 4 cut(s) 77, 111, 159, 519
FspBI CTAG 1 cut(s) 537
GluI GCNGC 4 cut(s) 77, 111, 159, 519
GsaI CCCAGC 3 cut(s) 131, 549, 1060
HaeIII GGCC 4 cut(s) 483, 810, 1017, 1051
HapII CCGG 2 cut(s) 209, 257
HgaI GACGC 1 cut(s) 634
Hin1II CATG 8 cut(s) 233, 278, 352, 368, 415, 515, 947, 1025
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaII CCGG 2 cut(s) 209, 257
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 6 cut(s) 138, 217, 438, 817, 888, 932
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 1 cut(s) 375
Hpy99I CGWCG 3 cut(s) 221, 379, 1050
HpyAV CCTTC 6 cut(s) 35, 115, 166, 325, 363, 800
HpyCH4III ACNGT 3 cut(s) 488, 667, 1010
HpyCH4V TGCA 7 cut(s) 417, 467, 511, 521, 604, 904, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 123, 311, 527, 1057
HpyF3I CTNAG 2 cut(s) 135, 289
Hsp92II CATG 8 cut(s) 233, 278, 352, 368, 415, 515, 947, 1025
Kzo9I GATC 2 cut(s) 121, 394
LmnI GCTCC 2 cut(s) 218, 852
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 1 cut(s) 637
MaeI CTAG 1 cut(s) 537
MaeIII GTNAC 2 cut(s) 749, 1004
MalI GATC 2 cut(s) 123, 396
MboI GATC 2 cut(s) 121, 394
MboII GAAGA 3 cut(s) 203, 785, 968
MfeI CAATTG 2 cut(s) 468, 891
MhlI GDGCHC 3 cut(s) 215, 552, 766
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 5 cut(s) 468, 821, 891, 971, 991
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 3 cut(s) 704, 762, 867
MmeI TCCRAC 1 cut(s) 240
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 822
MscI TGGCCA 1 cut(s) 483
MseI TTAA 1 cut(s) 725
MslI CAYNNNNRTG 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 483
MspI CCGG 2 cut(s) 209, 257
MspR9I CCNGG 2 cut(s) 210, 1038
MunI CAATTG 2 cut(s) 468, 891
MvaI CCWGG 1 cut(s) 1038
MwoI GCNNNNNNNGC 5 cut(s) 110, 123, 311, 527, 1057
NciI CCSGG 1 cut(s) 210
NcoI CCATGG 1 cut(s) 348
NdeI CATATG 1 cut(s) 600
NdeII GATC 2 cut(s) 121, 394
NlaIII CATG 8 cut(s) 233, 278, 352, 368, 415, 515, 947, 1025
NlaIV GGNNCC 2 cut(s) 214, 255
NspI RCATGY 2 cut(s) 515, 947
PaeI GCATGC 1 cut(s) 947
PaeR7I CTCGAG 1 cut(s) 336
PceI AGGCCT 1 cut(s) 810
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 7 cut(s) 5, 44, 455, 595, 659, 737, 1033
PflFI GACNNNGTC 1 cut(s) 1044
PflMI CCANNNNNTGG 2 cut(s) 490, 922
PfoI TCCNGGA 1 cut(s) 1036
PkrI GCNGC 4 cut(s) 78, 112, 160, 520
Ple19I CGATCG 1 cut(s) 124
PleI GAGTC 3 cut(s) 703, 762, 867
PpsI GAGTC 3 cut(s) 703, 762, 867
Psp6I CCWGG 1 cut(s) 1036
PspFI CCCAGC 3 cut(s) 127, 545, 1056
PspGI CCWGG 1 cut(s) 1036
PspN4I GGNNCC 2 cut(s) 214, 255
PspPI GGNCC 2 cut(s) 254, 1016
PspXI VCTCGAGB 1 cut(s) 336
PstNI CAGNNNCTG 2 cut(s) 506, 716
PsyI GACNNNGTC 1 cut(s) 1044
PvuI CGATCG 1 cut(s) 124
RsaI GTAC 2 cut(s) 714, 963
RsaNI GTAC 2 cut(s) 713, 962
RseI CAYNNNNRTG 1 cut(s) 279
SalI GTCGAC 1 cut(s) 373
SaqAI TTAA 1 cut(s) 725
SatI GCNGC 4 cut(s) 77, 111, 159, 519
Sau3AI GATC 2 cut(s) 121, 394
Sau96I GGNCC 2 cut(s) 254, 1016
SchI GAGTC 3 cut(s) 704, 762, 867
ScrFI CCNGG 2 cut(s) 210, 1038
SduI GDGCHC 3 cut(s) 215, 552, 766
SfaNI GCATC 1 cut(s) 637
Sfr274I CTCGAG 1 cut(s) 336
SinI GGWCC 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 2 cut(s) 336, 355
SmoI CTYRAG 2 cut(s) 336, 355
SphI GCATGC 1 cut(s) 947
Sse9I AATT 5 cut(s) 468, 821, 891, 971, 991
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 5 cut(s) 77, 102, 111, 115, 159
SspI AATATT 1 cut(s) 788
SspMI CTAG 1 cut(s) 537
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 2 cut(s) 208, 1036
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 3 cut(s) 488, 667, 1010
TaqI TCGA 6 cut(s) 183, 337, 374, 662, 771, 876
TaqII GACCGA 1 cut(s) 885
TasI AATT 5 cut(s) 468, 821, 891, 971, 991
TauI GCSGC 3 cut(s) 79, 113, 161
TfiI GAWTC 7 cut(s) 5, 44, 455, 595, 659, 737, 1033
Tru1I TTAA 1 cut(s) 725
Tru9I TTAA 1 cut(s) 725
TseI GCWGC 1 cut(s) 518
TspDTI ATGAA 8 cut(s) 218, 288, 396, 587, 819, 819, 984, 985
TspGWI ACGGA 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 1044
Van91I CCANNNNNTGG 2 cut(s) 490, 922
VpaK11BI GGWCC 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 29
XapI RAATTY 3 cut(s) 821, 971, 991
XceI RCATGY 2 cut(s) 515, 947
XhoI CTCGAG 1 cut(s) 336
XmiI GTMKAC 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 822
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.