RchiOBHm_Chr6g0295911

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011016
Physical Location & Seq
Reverse (-)
57772632 .. 57775153
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 657 bp
ATGGAGACCCTTTACCAATACTCAATCTGCCAAGAAGAACAAAACCATGCTTTCCCCAATACCCAGTTGCTCTCCTGCGACTGTTTCATCTTTAACCTCCACTTCACTCATCAGCTTCAATTGTACTACCCCCAAGATTTGTTCATACCCTTGAACTCAATCCAAGAATCCTTCTTTGTACCCTGTAACGTGTTTTTCACACATACCCATGACACCATCAACACCATTCTCTCTGGGACAGGTGTCTCCCTGGACTTCATTCAGGCTTTGGTCCCTCAAGTTTACTCCTTTGCTTTGGAGATTGCCACTGAGTTTGAAAATGGGGACCCTGATGAGCCTAAAGCGGTCCCTTTGAATTTGGGTGTTGTGGCTGTCACGCCCTATGATGACCATGACCAGATTGAAAGGGCTATTTGGGAATCTGGGCAGGTTTTTAATCCGGTTCCGGCCACCAGGTCGTCTATTGCGGCATTGAAGAAGGTCAAGGTTGAGAATTTGAGTGTGGTGAATGAATGTAGTATCTGTTTGGAGGAGTTTTCCACTGGGTTGGAGGTTATACGCATGCCCTGCTCCCATGTTTATCATAAGGATTGCATTGTTGAGTGGTTGAAGAGGAGTCACTTGTGCCCGTTGTGCCGGTTTTCGATGCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

218

Amino Acids

24.82

Weight (kDa)

4.79

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 168 - 213 4.7e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 170 - 213 9.4e-16 Ring finger domain
zf-RING_11 PF17123 171 - 199 1.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 171 - 212 2.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 171 - 213 8e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 172 - 210 4.5e-08 RING-type zinc-finger
zf-C3HC4 PF00097 172 - 212 7.2e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18760 AT1G18770 AT1G18780 AT1G21960 AT2G29840 AT3G13228
fragaria_vesca FvH4_2g27040
malus_domestica MD08G1162100.v1.1
prunus_persica Prupe.1G495200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295911
rosa_laevigata RLG00000011757
rosa_multiflora Rmu_sc0003139.1_g000008
rosa_roxburghii Rroxscaffold_7G00172060
rosa_rugosa Rorug06G0255000 Rorug06G0255000
rosa_samantha Rh6AG368800 Rh6BG376500 Rh6CG382700 Rh6DG369300
rosa_wichuraiana Rw6G032160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 418
AciI CCGC 2 cut(s) 344, 467
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 2 cut(s) 355, 493
AfaI GTAC 2 cut(s) 125, 180
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 7 cut(s) 119, 154, 317, 355, 404, 475, 610
AjnI CCWGG 2 cut(s) 249, 452
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 250
AoxI GGCC 1 cut(s) 447
ApoI RAATTY 2 cut(s) 355, 493
AspS9I GGNCC 3 cut(s) 271, 325, 346
AsuHPI GGTGA 1 cut(s) 517
AvaII GGWCC 3 cut(s) 271, 325, 346
BaeGI GKGCMC 1 cut(s) 629
BccI CCATC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 251, 454
BcoDI GTCTC 1 cut(s) 250
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 251, 454
Bme18I GGWCC 3 cut(s) 271, 325, 346
BmgT120I GGNCC 3 cut(s) 271, 325, 346
BmiI GGNNCC 5 cut(s) 273, 326, 327, 348, 444
BmrFI CCNGG 2 cut(s) 251, 454
BmrI ACTGGG 2 cut(s) 58, 552
BmsI GCATC 1 cut(s) 636
BmuI ACTGGG 2 cut(s) 58, 552
BoxI GACNNNNGTC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 2 cut(s) 64, 547
BseBI CCWGG 2 cut(s) 251, 454
BseDI CCNNGG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 64, 547
BseRI GAGGAG 2 cut(s) 545, 628
BseSI GKGCMC 1 cut(s) 629
BshFI GGCC 1 cut(s) 449
BsiSI CCGG 3 cut(s) 440, 446, 637
BslFI GGGAC 4 cut(s) 250, 257, 332, 338
BsmAI GTCTC 1 cut(s) 250
BsmFI GGGAC 4 cut(s) 250, 257, 332, 338
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 629
BspACI CCGC 2 cut(s) 344, 467
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 5 cut(s) 273, 326, 327, 348, 444
BspMI ACCTGC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 636
BsrI ACTGG 2 cut(s) 64, 547
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 1 cut(s) 249
Bst2UI CCWGG 2 cut(s) 251, 454
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 567
BstC8I GCNNGC 1 cut(s) 563
BstDEI CTNAG 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 250
BstMWI GCNNNNNNNGC 2 cut(s) 567, 633
BstNI CCWGG 2 cut(s) 251, 454
BstNSI RCATGY 1 cut(s) 565
BstPAI GACNNNNGTC 1 cut(s) 242
BstSCI CCNGG 2 cut(s) 249, 452
BstSLI GKGCMC 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 547
BsuRI GGCC 1 cut(s) 449
BtsIMutI CAGTG 2 cut(s) 306, 540
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 636
Cfr13I GGNCC 3 cut(s) 271, 325, 346
CsiI ACCWGGT 1 cut(s) 452
Csp6I GTAC 2 cut(s) 124, 179
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 5 cut(s) 47, 209, 392, 562, 575
CviJI RGCY 6 cut(s) 115, 266, 337, 371, 410, 449
CviKI_1 RGCY 6 cut(s) 115, 266, 337, 371, 410, 449
CviQI GTAC 2 cut(s) 124, 179
DdeI CTNAG 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Eco47I GGWCC 3 cut(s) 271, 325, 346
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 2 cut(s) 249, 452
FaeI CATG 5 cut(s) 50, 212, 395, 565, 578
FaqI GGGAC 4 cut(s) 250, 257, 332, 338
FatI CATG 5 cut(s) 46, 208, 391, 561, 574
Fnu4HI GCNGC 1 cut(s) 468
Fsp4HI GCNGC 1 cut(s) 468
GluI GCNGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 449
HapII CCGG 3 cut(s) 440, 446, 637
Hin1II CATG 5 cut(s) 50, 212, 395, 565, 578
HinfI GANTC 3 cut(s) 167, 419, 616
HpaII CCGG 3 cut(s) 440, 446, 637
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 2 cut(s) 181, 472
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 567, 633
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 5 cut(s) 50, 212, 395, 565, 578
KflI GGGWCCC 1 cut(s) 325
LmnI GCTCC 1 cut(s) 575
LweI GCATC 1 cut(s) 636
MabI ACCWGGT 1 cut(s) 452
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 185, 373, 617
MboII GAAGA 3 cut(s) 47, 487, 622
MfeI CAATTG 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 3 cut(s) 119, 355, 493
MlyI GAGTC 1 cut(s) 625
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 5 cut(s) 107, 285, 523, 544, 606
MseI TTAA 2 cut(s) 93, 435
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 3 cut(s) 440, 446, 637
MspR9I CCNGG 2 cut(s) 251, 454
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 2 cut(s) 251, 454
MwoI GCNNNNNNNGC 2 cut(s) 567, 633
NlaIII CATG 5 cut(s) 50, 212, 395, 565, 578
NlaIV GGNNCC 5 cut(s) 273, 326, 327, 348, 444
NmuCI GTSAC 2 cut(s) 373, 617
NspI RCATGY 1 cut(s) 565
PaeI GCATGC 1 cut(s) 565
PfeI GAWTC 2 cut(s) 167, 419
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 1 cut(s) 624
PpsI GAGTC 1 cut(s) 624
PpuMI RGGWCCY 1 cut(s) 325
PshAI GACNNNNGTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 249, 452
PspGI CCWGG 2 cut(s) 249, 452
PspN4I GGNNCC 5 cut(s) 273, 326, 327, 348, 444
PspPI GGNCC 3 cut(s) 271, 325, 346
PspPPI RGGWCCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 125, 180
RsaNI GTAC 2 cut(s) 124, 179
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 2 cut(s) 93, 435
SatI GCNGC 1 cut(s) 468
Sau96I GGNCC 3 cut(s) 271, 325, 346
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 2 cut(s) 251, 454
SduI GDGCHC 1 cut(s) 629
SetI ASST 9 cut(s) 99, 117, 192, 244, 432, 458, 483, 489, 555
SexAI ACCWGGT 1 cut(s) 452
SfaNI GCATC 1 cut(s) 636
SinI GGWCC 3 cut(s) 271, 325, 346
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
SphI GCATGC 1 cut(s) 565
Sse9I AATT 3 cut(s) 119, 355, 493
SsiI CCGC 2 cut(s) 344, 467
StyD4I CCNGG 2 cut(s) 249, 452
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 192
TaqI TCGA 1 cut(s) 644
TasI AATT 3 cut(s) 119, 355, 493
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 2 cut(s) 167, 419
Tru1I TTAA 2 cut(s) 93, 435
Tru9I TTAA 2 cut(s) 93, 435
TscAI CASTG 2 cut(s) 313, 547
TseFI GTSAC 2 cut(s) 373, 617
Tsp45I GTSAC 2 cut(s) 373, 617
TspDTI ATGAA 4 cut(s) 76, 133, 247, 525
TspRI CASTG 2 cut(s) 313, 547
VpaK11BI GGWCC 3 cut(s) 271, 325, 346
XapI RAATTY 2 cut(s) 355, 493
XceI RCATGY 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.