RchiOBHm_Chr5g0044211

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014974
Physical Location & Seq
Forward (+)
39463014 .. 39464261
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGGCGGAAGAGCATAGATGCCAGGCGCCGAAGCTGTGCGTGAACAACTGCGGATTCTTCGGGAGCCCAACCACGCAAAACCTGTGCTCCAAATGCTACCGTGATCTGCAGCACAAAGAGCAACAAGCCCTCGCTCTCAATCAAACCTTCATCTCAGCTTCGTCCACCTCTTCTTCTTCTTCGGCTTTCTTCCCTTCGTCGCCGGTCTCGGTCTCGGTCTCGTCGCCGCAGAACGAGGGCGCGGCCGAGGACGCGACGAAGGTAGAGGAGGAGAAGAAGAAGAAGGAGGAAGCGGTGCCCGGGACGGCGGGGAGGCAGGCGAACAGGTGCACGGAGTGCAGGCGGCGCGTGGGGTTGACGGGGTTCAAGTGCAGGTGCGGGATGACGTACTGCGGGACCCACAGGTACCCGGAGAAGCACGAGTGCGGGTTCGACTTCAGGAGCATGGGGAGGGAGCAGATCGCCAAGGCCAATCCGGTCGTCAAGGGCGACAAGCTGCAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

169

Amino Acids

18.59

Weight (kDa)

8.89

Isoelectric Point (pI)

62.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 12 - 34 1e-12 A20-like zinc finger
zf-AN1 PF01428 110 - 145 7.8e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27580 AT2G27580
fragaria_vesca FvH4_3g24650
malus_domestica MD03G1142300.v1.1 MD11G1162800.v1.1
prunus_persica Prupe.6G127400_v2.0.a1
pyrus_communis pycom03g09700
rosa_chinensis RchiOBHm_Chr5g0044211
rosa_laevigata RLG00000034253
rosa_multiflora Rmu_sc0000776.1_g000081 Rmu_sc0002731.1_g000003
rosa_roxburghii Rroxscaffold_1G00036830
rosa_rugosa Rorug05G0211700
rosa_samantha Rh5BG304100 Rh5DG312800
rosa_wichuraiana Rw5G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 363
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 3 cut(s) 25, 295, 405
AccII CGCG 3 cut(s) 242, 254, 348
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 421
AcyI GRCGYC 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 2 cut(s) 389, 407
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 3 cut(s) 34, 158, 496
AluI AGCT 3 cut(s) 34, 158, 496
Alw21I GWGCWC 2 cut(s) 89, 332
Alw26I GTCTC 3 cut(s) 211, 217, 223
Alw44I GTGCAC 1 cut(s) 328
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 2 cut(s) 243, 468
ApaLI GTGCAC 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 109, 496
Asp718I GGTACC 1 cut(s) 405
AspLEI GCGC 3 cut(s) 28, 242, 348
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 3 cut(s) 300, 301, 410
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 396
BaeGI GKGCMC 2 cut(s) 300, 332
BanI GGYRCC 3 cut(s) 25, 295, 405
BanII GRGCYC 1 cut(s) 68
BauI CACGAG 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 89, 332
BbvI GCAGC 2 cut(s) 121, 483
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 3 cut(s) 300, 301, 410
BcoDI GTCTC 3 cut(s) 211, 217, 223
BfmI CTRYAG 2 cut(s) 107, 497
BfoI RGCGCY 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 363
BglII AGATCT 1 cut(s) 504
BisI GCNGC 5 cut(s) 110, 227, 243, 344, 497
BlsI GCNGC 5 cut(s) 111, 228, 244, 345, 498
Bme1390I CCNGG 4 cut(s) 23, 300, 301, 410
Bme18I GGWCC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 6 cut(s) 27, 65, 297, 397, 398, 407
BmrFI CCNGG 4 cut(s) 23, 300, 301, 410
BmsI GCATC 1 cut(s) 8
BpuMI CCSGG 3 cut(s) 300, 301, 410
BsaHI GRCGYC 1 cut(s) 26
BsaI GGTCTC 3 cut(s) 211, 217, 223
BsaJI CCNNGG 3 cut(s) 246, 299, 465
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse118I RCCGGY 1 cut(s) 202
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 246, 299, 465
BseGI GGATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 2 cut(s) 281, 284
BseSI GKGCMC 2 cut(s) 300, 332
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 121, 483
BsgI GTGCAG 2 cut(s) 358, 391
Bsh1236I CGCG 3 cut(s) 242, 254, 348
Bsh1285I CGRYCG 2 cut(s) 246, 480
BshFI GGCC 2 cut(s) 245, 470
BshNI GGYRCC 3 cut(s) 25, 295, 405
BsiEI CGRYCG 2 cut(s) 246, 480
BsiHKAI GWGCWC 2 cut(s) 89, 332
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 203, 300, 410, 476
BslFI GGGAC 2 cut(s) 316, 409
BsmAI GTCTC 3 cut(s) 211, 217, 223
BsmFI GGGAC 2 cut(s) 316, 409
BsnI GGCC 2 cut(s) 245, 470
Bso31I GGTCTC 3 cut(s) 211, 217, 223
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 4 cut(s) 68, 89, 300, 332
Bsp143I GATC 3 cut(s) 103, 459, 504
BspANI GGCC 2 cut(s) 245, 470
BspCNI CTCAG 1 cut(s) 167
BspFNI CGCG 3 cut(s) 242, 254, 348
BspLI GGNNCC 6 cut(s) 27, 65, 297, 397, 398, 407
BspMAI CTGCAG 2 cut(s) 111, 501
BspMI ACCTGC 1 cut(s) 363
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 3 cut(s) 25, 295, 405
BspTNI GGTCTC 3 cut(s) 211, 217, 223
BsrFI RCCGGY 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 3 cut(s) 246, 299, 465
BssMI GATC 3 cut(s) 103, 459, 504
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 3, 175
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 318, 341
BstDEI CTNAG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 387
BstFNI CGCG 3 cut(s) 242, 254, 348
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 3 cut(s) 28, 242, 348
BstKTI GATC 3 cut(s) 106, 462, 507
BstMAI GTCTC 3 cut(s) 211, 217, 223
BstMBI GATC 3 cut(s) 103, 459, 504
BstMCI CGRYCG 2 cut(s) 246, 480
BstMWI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 336, 345
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 4 cut(s) 21, 298, 299, 408
BstSFI CTRYAG 2 cut(s) 107, 497
BstSLI GKGCMC 2 cut(s) 300, 332
BstUI CGCG 3 cut(s) 242, 254, 348
BstV1I GCAGC 2 cut(s) 121, 483
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 245, 470
BtsCI GGATG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 363
Cac8I GCNNGC 2 cut(s) 318, 341
CfoI GCGC 3 cut(s) 28, 242, 348
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 396
Cfr9I CCCGGG 1 cut(s) 299
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 388, 406
CviAII CATG 1 cut(s) 445
CviJI RGCY 8 cut(s) 34, 66, 128, 158, 185, 245, 470, 496
CviKI_1 RGCY 8 cut(s) 34, 66, 128, 158, 185, 245, 470, 496
CviQI GTAC 2 cut(s) 388, 406
DdeI CTNAG 1 cut(s) 154
DinI GGCGCC 1 cut(s) 27
DpnI GATC 3 cut(s) 105, 461, 506
DpnII GATC 3 cut(s) 103, 459, 504
DraIII CACNNNGTG 1 cut(s) 336
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 3, 175
EarI CTCTTC 2 cut(s) 3, 175
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 68
Eco31I GGTCTC 3 cut(s) 211, 217, 223
Eco47I GGWCC 1 cut(s) 396
Eco52I CGGCCG 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 68
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 448
FaiI YATR 2 cut(s) 15, 446
FaqI GGGAC 2 cut(s) 316, 409
FatI CATG 1 cut(s) 444
FauI CCCGC 4 cut(s) 301, 371, 386, 419
Fnu4HI GCNGC 5 cut(s) 110, 227, 243, 344, 497
FokI GGATG 1 cut(s) 394
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 5 cut(s) 110, 227, 243, 344, 497
GlaI GCGC 3 cut(s) 27, 241, 347
GluI GCNGC 5 cut(s) 110, 227, 243, 344, 497
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 245, 470
HapII CCGG 4 cut(s) 203, 300, 410, 476
HgaI GACGC 1 cut(s) 260
HhaI GCGC 3 cut(s) 28, 242, 348
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 1 cut(s) 448
Hin6I GCGC 3 cut(s) 26, 240, 346
HinP1I GCGC 3 cut(s) 26, 240, 346
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 1 cut(s) 54
HpaII CCGG 4 cut(s) 203, 300, 410, 476
Hpy166II GTNNAC 4 cut(s) 43, 165, 330, 357
Hpy188I TCNGA 1 cut(s) 509
Hpy188III TCNNGA 2 cut(s) 61, 439
Hpy8I GTNNAC 4 cut(s) 43, 165, 330, 357
Hpy99I CGWCG 3 cut(s) 202, 226, 259
HpyAV CCTTC 4 cut(s) 157, 204, 253, 277
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 5 cut(s) 109, 330, 339, 372, 499
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 336, 345
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 1 cut(s) 448
HspAI GCGC 3 cut(s) 26, 240, 346
KasI GGCGCC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 396
KpnI GGTACC 1 cut(s) 409
Kzo9I GATC 3 cut(s) 103, 459, 504
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 4 cut(s) 63, 92, 441, 454
Lsp1109I GCAGC 2 cut(s) 121, 483
LweI GCATC 1 cut(s) 8
MaeII ACGT 1 cut(s) 386
MalI GATC 3 cut(s) 105, 461, 506
MboI GATC 3 cut(s) 103, 459, 504
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 4 cut(s) 68, 89, 300, 332
Mly113I GGCGCC 1 cut(s) 26
MnlI CCTC 9 cut(s) 140, 178, 229, 241, 259, 262, 280, 306, 444
MspI CCGG 4 cut(s) 203, 300, 410, 476
MspR9I CCNGG 4 cut(s) 23, 300, 301, 410
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 3 cut(s) 242, 254, 348
MwoI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 336, 345
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 3 cut(s) 300, 301, 410
NdeII GATC 3 cut(s) 103, 459, 504
NlaIII CATG 1 cut(s) 448
NlaIV GGNNCC 6 cut(s) 27, 65, 297, 397, 398, 407
NmeAIII GCCGAG 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 5 cut(s) 111, 228, 244, 345, 498
PluTI GGCGCC 1 cut(s) 29
PpuMI RGGWCCY 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 6 cut(s) 27, 65, 297, 397, 398, 407
PspPI GGNCC 1 cut(s) 396
PspPPI RGGWCCY 1 cut(s) 396
PstI CTGCAG 2 cut(s) 111, 501
PsuI RGATCY 1 cut(s) 504
RsaI GTAC 2 cut(s) 389, 407
RsaNI GTAC 2 cut(s) 388, 406
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 5 cut(s) 110, 227, 243, 344, 497
Sau3AI GATC 3 cut(s) 103, 459, 504
Sau96I GGNCC 1 cut(s) 396
ScrFI CCNGG 4 cut(s) 23, 300, 301, 410
SduI GDGCHC 4 cut(s) 68, 89, 300, 332
SfaNI GCATC 1 cut(s) 8
SfcI CTRYAG 2 cut(s) 107, 497
SfoI GGCGCC 1 cut(s) 27
SinI GGWCC 1 cut(s) 396
SmaI CCCGGG 1 cut(s) 301
SspDI GGCGCC 1 cut(s) 25
StyD4I CCNGG 4 cut(s) 21, 298, 299, 408
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 389
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 2 cut(s) 199, 205
TauI GCSGC 3 cut(s) 229, 245, 346
TfiI GAWTC 1 cut(s) 54
TseI GCWGC 2 cut(s) 109, 496
TspDTI ATGAA 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 347
TspMI CCCGGG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 396
XmaI CCCGGG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.