RchiOBHm_Chr5g0054371

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013350
Physical Location & Seq
Reverse (-)
56976073 .. 56978169
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGGAGTTGGGTGTTTTTAGTGCTTCTGTTGCTGCTGGTGCTGGTTTAGTACTACTGGTTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATATGCAAGGAGTTATATCAGCTGGGATGATTTGAAGGTGGATGAGCAGATGGTCACTGGTTTGAACTCTGGAAATGAAGAGCAAAACGGGACACGCGTCATTGTGGTGGACAAGAATGGAGAAGGAAACTCAATGACAGTTCAAGCAGCAGTTGACATGGTTCCACACCATAACACCCAAAGAGTCAAAATTTACATTCTTCCAGGAATTTATAGAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTAAGACTGTAATTACTTGGAACAGTAAGGCATCTGATAAGGACAGCAATGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGCGGTTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGTAAGGGTTTTGGGAGCACAAGATACATTATTGGATGAGAATGGATCGCACTACTTCTACGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGAAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAACTGCTAAGACCTACGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTCATATATTCCTTTTGTCATATTGACAATATAATAACTCCTCTGGGATGGAGTGACTGGAACATGCCACCAAGGCAGAGGACTGTGGAATTCGGAGAATACCAATGCAGAGGCAAAGGAGCAGATAGAAGAGGCAGGGTACCATGGTTGAAATCTTTTAGTCCTGACGAGATAACCCCTTTTCTGGATACCAAATTCATATATGGAGAGCAATGGCTTAGACTATAG

Protein Analysis

366

Amino Acids

40.62

Weight (kDa)

6.01

Isoelectric Point (pI)

27.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 360 1e-65 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1012
AccB1I GGYRCC 1 cut(s) 1012
AccII CGCG 1 cut(s) 198
AciI CCGC 1 cut(s) 521
AclWI GGATC 2 cut(s) 509, 649
AcsI RAATTY 4 cut(s) 291, 309, 962, 1067
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 3 cut(s) 51, 452, 1014
AfiI CCNNNNNNNGG 1 cut(s) 1057
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 7 cut(s) 136, 166, 245, 440, 671, 870, 1024
AjnI CCWGG 3 cut(s) 304, 532, 714
AluBI AGCT 4 cut(s) 123, 764, 854, 863
AluI AGCT 4 cut(s) 123, 764, 854, 863
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 2 cut(s) 509, 649
AlwNI CAGNNNCTG 2 cut(s) 464, 533
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 32, 248, 761
ApoI RAATTY 4 cut(s) 291, 309, 962, 1067
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp718I GGTACC 1 cut(s) 1012
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 771
AvaII GGWCC 1 cut(s) 367
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BanI GGYRCC 1 cut(s) 1012
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 3 cut(s) 19, 260, 773
BccI CCATC 2 cut(s) 145, 915
BcgI CGANNNNNNTGC 4 cut(s) 500, 534, 805, 839
BciT130I CCWGG 3 cut(s) 306, 534, 716
BciVI GTATCC 1 cut(s) 1054
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 3 cut(s) 465, 592, 1097
BfuI GTATCC 1 cut(s) 1054
BglI GCCNNNNNGGC 2 cut(s) 476, 946
BisI GCNGC 3 cut(s) 33, 249, 762
BlsI GCNGC 3 cut(s) 34, 250, 763
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 306, 534, 716
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 3 cut(s) 264, 368, 1014
BmrFI CCNGG 3 cut(s) 306, 534, 716
BmsI GCATC 1 cut(s) 422
BoxI GACNNNNGTC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 555, 852
BsaJI CCNNGG 2 cut(s) 944, 1016
Bsc4I CCNNNNNNNGG 1 cut(s) 1057
Bse1I ACTGG 4 cut(s) 60, 163, 835, 935
Bse3DI GCAATG 2 cut(s) 436, 1091
BseBI CCWGG 3 cut(s) 306, 534, 716
BseDI CCNNGG 2 cut(s) 944, 1016
BseGI GGATG 4 cut(s) 133, 148, 637, 926
BseLI CCNNNNNNNGG 1 cut(s) 1057
BseMI GCAATG 2 cut(s) 436, 1091
BseNI ACTGG 4 cut(s) 60, 163, 835, 935
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 3 cut(s) 19, 260, 773
BseYI CCCAGC 2 cut(s) 123, 572
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 616
BslFI GGGAC 2 cut(s) 205, 380
BslI CCNNNNNNNGG 1 cut(s) 1057
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 2 cut(s) 205, 380
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 2 cut(s) 501, 641
Bsp19I CCATGG 1 cut(s) 1016
BspACI CCGC 1 cut(s) 521
BspANI GGCC 1 cut(s) 584
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 264, 368, 1014
BspPI GGATC 2 cut(s) 509, 649
BspQI GCTCTTC 2 cut(s) 174, 844
BspT107I GGYRCC 1 cut(s) 1012
BsrDI GCAATG 2 cut(s) 436, 1091
BsrI ACTGG 4 cut(s) 60, 163, 835, 935
BssECI CCNNGG 2 cut(s) 944, 1016
BssMI GATC 2 cut(s) 501, 641
BssT1I CCWWGG 2 cut(s) 944, 1016
Bst2UI CCWGG 3 cut(s) 306, 534, 716
Bst4CI ACNGT 7 cut(s) 241, 391, 407, 466, 518, 722, 958
Bst6I CTCTTC 3 cut(s) 174, 844, 997
BstDEI CTNAG 4 cut(s) 384, 563, 741, 1091
BstDSI CCRYGG 1 cut(s) 1016
BstF5I GGATG 4 cut(s) 133, 148, 637, 926
BstFNI CGCG 1 cut(s) 198
BstKTI GATC 2 cut(s) 504, 644
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 2 cut(s) 501, 641
BstMWI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
BstNI CCWGG 3 cut(s) 306, 534, 716
BstNSI RCATGY 1 cut(s) 940
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 304, 532, 714
BstSFI CTRYAG 3 cut(s) 465, 592, 1097
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 19, 260, 773
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 1 cut(s) 584
BtgI CCRYGG 1 cut(s) 1016
BtsCI GGATG 4 cut(s) 133, 148, 637, 926
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 3 cut(s) 156, 523, 534
CaiI CAGNNNCTG 2 cut(s) 464, 533
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 1 cut(s) 187
Csp6I GTAC 3 cut(s) 50, 451, 1013
CviAII CATG 4 cut(s) 259, 586, 937, 1017
CviQI GTAC 3 cut(s) 50, 451, 1013
DdeI CTNAG 4 cut(s) 384, 563, 741, 1091
DpnI GATC 2 cut(s) 503, 643
DpnII GATC 2 cut(s) 501, 641
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 174, 844, 997
EarI CTCTTC 3 cut(s) 174, 844, 997
Eco130I CCWWGG 2 cut(s) 944, 1016
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 367
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 3 cut(s) 304, 532, 714
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 944, 1016
ErhI CCWWGG 2 cut(s) 944, 1016
FaeI CATG 4 cut(s) 262, 589, 940, 1020
FaqI GGGAC 2 cut(s) 205, 380
FatI CATG 4 cut(s) 258, 585, 936, 1016
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 33, 249, 762
FokI GGATG 4 cut(s) 140, 155, 644, 933
Fsp4HI GCNGC 3 cut(s) 33, 249, 762
FspBI CTAG 1 cut(s) 443
GluI GCNGC 3 cut(s) 33, 249, 762
GsaI CCCAGC 2 cut(s) 127, 576
GsuI CTGGAG 2 cut(s) 555, 852
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 1 cut(s) 187
Hin1II CATG 4 cut(s) 262, 589, 940, 1020
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 5 cut(s) 285, 373, 659, 776, 873
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 4 cut(s) 211, 256, 682, 811
Hpy188I TCNGA 3 cut(s) 418, 827, 968
Hpy188III TCNNGA 6 cut(s) 171, 511, 808, 870, 1037, 1058
Hpy8I GTNNAC 4 cut(s) 211, 256, 682, 811
HpyAV CCTTC 3 cut(s) 130, 218, 316
HpyCH4III ACNGT 7 cut(s) 241, 391, 407, 466, 518, 722, 958
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 7 cut(s) 107, 494, 527, 550, 761, 816, 981
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
HpyF3I CTNAG 4 cut(s) 384, 563, 741, 1091
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 4 cut(s) 262, 589, 940, 1020
KpnI GGTACC 1 cut(s) 1016
Kzo9I GATC 2 cut(s) 501, 641
LguI GCTCTTC 2 cut(s) 174, 844
LmnI GCTCC 4 cut(s) 611, 752, 860, 992
Lsp1109I GCAGC 3 cut(s) 19, 260, 773
LweI GCATC 1 cut(s) 422
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 3 cut(s) 154, 705, 926
MalI GATC 2 cut(s) 503, 643
MboI GATC 2 cut(s) 501, 641
MboII GAAGA 5 cut(s) 191, 293, 800, 861, 1014
MfeI CAATTG 1 cut(s) 756
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 7 cut(s) 291, 309, 336, 393, 756, 962, 1067
MluI ACGCGT 1 cut(s) 196
MlyI GAGTC 3 cut(s) 294, 770, 882
MnlI CCTC 6 cut(s) 469, 530, 924, 945, 977, 998
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 123
MspR9I CCNGG 3 cut(s) 306, 534, 716
MunI CAATTG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 3 cut(s) 306, 534, 716
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
NcoI CCATGG 1 cut(s) 1016
NdeI CATATG 1 cut(s) 103
NdeII GATC 2 cut(s) 501, 641
NlaIII CATG 4 cut(s) 262, 589, 940, 1020
NlaIV GGNNCC 3 cut(s) 264, 368, 1014
NmuCI GTSAC 3 cut(s) 154, 705, 926
NspI RCATGY 1 cut(s) 940
PciSI GCTCTTC 2 cut(s) 174, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 373, 659
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 3 cut(s) 34, 250, 763
PleI GAGTC 3 cut(s) 293, 770, 881
PpsI GAGTC 3 cut(s) 293, 770, 881
PshAI GACNNNNGTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 304, 532, 714
PspFI CCCAGC 2 cut(s) 123, 572
PspGI CCWGG 3 cut(s) 304, 532, 714
PspN4I GGNNCC 3 cut(s) 264, 368, 1014
PspPI GGNCC 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 464, 533
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 3 cut(s) 51, 452, 1014
RsaNI GTAC 3 cut(s) 50, 451, 1013
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 174, 844
SatI GCNGC 3 cut(s) 33, 249, 762
Sau3AI GATC 2 cut(s) 501, 641
Sau96I GGNCC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 3 cut(s) 294, 770, 882
ScrFI CCNGG 3 cut(s) 306, 534, 716
SduI GDGCHC 1 cut(s) 616
SetI ASST 9 cut(s) 125, 141, 327, 452, 707, 749, 766, 856, 865
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 3 cut(s) 465, 592, 1097
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 7 cut(s) 291, 309, 336, 393, 756, 962, 1067
SsiI CCGC 1 cut(s) 521
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 304, 532, 714
StyI CCWWGG 2 cut(s) 944, 1016
TaaI ACNGT 7 cut(s) 241, 391, 407, 466, 518, 722, 958
TaiI ACGT 1 cut(s) 452
TaqI TCGA 2 cut(s) 376, 510
TasI AATT 7 cut(s) 291, 309, 336, 393, 756, 962, 1067
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 2 cut(s) 373, 659
TscAI CASTG 3 cut(s) 163, 523, 534
TseFI GTSAC 3 cut(s) 154, 705, 926
TseI GCWGC 3 cut(s) 32, 248, 761
Tsp45I GTSAC 3 cut(s) 154, 705, 926
TspDTI ATGAA 4 cut(s) 192, 676, 801, 1060
TspGWI ACGGA 1 cut(s) 765
TspRI CASTG 3 cut(s) 163, 523, 534
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 4 cut(s) 291, 309, 962, 1067
XceI RCATGY 1 cut(s) 940
XspI CTAG 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.