Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010089

Physical Location & Seq

Genomic Coordinates Forward (+)

60767819 .. 60775866

Gene Structure 8048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCTCTCACTCTCACACCCACTGCGTTCTTGCTCTACTTGTCTTCGCCTACACTCTGCTCTCCGCTCTCTCCGTCACTCCTCTCTCTTCCCCATTCCGATTTCGCCAAACCCTAATCGCGCCATGTCCTCCAATCGCCCCTCCGCCTTCGACGCTCTCATGTCCGGCGCACGCGCCGCCGCCGCCAAGAAAAAGCCCCAACCCTCTTCCTCTTCCTCCCCCAAGAAGCGCAAAACCCTAAACCCCAGCCCTAGCTCCAATTCCGCGCCCAAGGAAAATCCCCAATTGCTATCCAAACCAGAAGACGCTGCTCCGAGCCCGGCCGCGGAATCCAAACCAGAAATGGAGGAAGAAGGTCAAGACCAGTCTGGCAATGGCGCCGAGCTGGCACCGACGGCGAAGAAAGTGTGCGCGGCGGCCGGAGTGGAGGAGCGGACGGCTCAATTGGCGGGTAGAATTGAGCTGTTGAAGAAGAAGGCCCTTGATTTTGACCCGAAGCTGATGGCGAATTGGGACAAGGGCGAAAGGGTTCCGTTTATGTTTCTTTCTTTGGTTTTCAATTTGATTTCCAAAGAGTCTGGGCGGATTGTGATTACGGATATAGTGTGTAATATGCTTAGGACTGTAATCTACACCACGCCGGAGGATTTGCTCCCGGTGGTTTATCTCTCGGCCAATAAGATTGCTCCGGCACATGAGGGATTGGAGCTTGGCATTGGGGACTCTTCAATTATCAAGGCGCTTTCGCAGGCTTGTGGGAGGAGTGAAAAGGACGTTAAGAAGCGCTATGATGCAGAAGGGGACTTGGGTATTGTTGCACAGAAAAGCCGTTCATCCCAAACGATGATGCGCAAGCCTGAGCCATTGACTGTCAGCAAGGTTTTCAACACATTTCGCACCCTTGCTAAGGAATCTGGGAAGGATAGCCAGGAGAAGAAAAAGAACCATATGAAGGCACTTCTTGTTGCTGCCACTGACTGTGAACCTCTGTATTTGATCCGTTTGCTTCAGTCGAAGTTGCGAATTGGATTGGCAGAGCAGACTCTTTTGGCTGCACTGGGACAAGCTGCAGTCTATACTGAAGAACATTCTAAACCACCTCCTGAAATTCAGTCTCCTTTAGAAGAGGCTGCAAAGATTGTTAAACAAGTGTACTCTCTGCTTCCTGTTTACGATAAGATCATCCCTGCCCTTCTGAGTGGTGGTGTTTGGAATCTTCCAAAAACGTGTAGCTTTACTTTGGGTATCCCAGTTGGACCTATGCTTGCAAAGCCAAGTAAAGGGGTATCTGAGATTGTCCAAAAGTTTCAAGATACAGAATACACCTGTGAATACAAGTATGATGGAGAGCGTGCCCAGATACATTACATGGAGGATGGTATTGTTGAGATATACAGTCGAAATGCTGAACGAAACACCGGAAAGTTCCCTGATGTTGCGATTGCAGTGTCAAGGTTAAAGAAGTCGTCTGTAAAATCATTTGTTTTAGATTGTGAATTGGTTGCCTATGACCGTGCAAATAACAAAATTCTGCCCTTCCAGGTACTTAGCACCCGAGCCCGTAAAAATGTGAGCATGAGCGATATTAAGATTGAGGTCTGCATATTTGCTTTTGATATGTTGTTTCTTAATGGTCGACCACTTACTCAAGAACAGCTCAAAGTTCGCAGAGAGTATCTTTATGACTCTTTTGAGGAAGAAGCTGGAATTTTTCAGTTTGCCACAGCAATAACCTCAAAGGATATTGATGAAATACAAACATTTCTTGATGCCGCCGTTAATTCAAGTTCTGAGGGTTTAATAATCAAAACATTAAGCAAAGATGCCACATACGAGCCTTCGAAGCGGTCACTTAACTGGCTGAAATTGAAGAAAGATTACATGGATAGTTTCGGGGACTCACTGGACTTGGTACCTATTGCTGCTTTCCATGGCCGGGGAAAACGTACAGGTGTCTATGGTGCTTTTCTCCTTGCTTGCTACGATAGCAATAATGAAGAGTTTCAAAGCATATGTAAAATAGGCACTGGATTTTCTGAAGCAGTGCTTGAAGAGCGTTCTGCCAGCCTGCGTGAAACAGTGATACCTAAACCTAAGACATACTATAGCTATGCAGAAACAAGTAATGCAAATACTCCAGATGTATGGTTTGAACCCACTCAGGTTTGGGAGGTGAAAGCTGCTGACTTGACTATTAGTCCTGTGTATCGTGCTGCAGTAGGCATAGTGGATTCTAACAAGGGTATTTCTCTTCGATTTCCACGTCTTGTTCGTGTTAGGGAAGATAAAAAACCAGAGGAAGCTTCATCATCTGAGCAGGTTGCTGATATGTATTCTGCTCAATCCAACAAAAGTTCTAATCACCAAGATGACAAGGAAGATGAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000012 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430

Pfam Domains

Protein Families

DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N

Protein Analysis

796

Amino Acids

87.99

Weight (kDa)

8.21

Isoelectric Point (pI)

53.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	178 - 353	3.5e-51	DNA ligase N terminus
DNA_ligase_A_M	PF01068	420 - 624	1.1e-64	ATP dependent DNA ligase domain
DNA_ligase_A_C	PF04679	649 - 763	1.5e-22	ATP dependent DNA ligase C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08130
fragaria_vesca	FvH4_3g30730 FvH4_3g30731 FvH4_3g30740
malus_domestica	MD03G1127000.v1.1 MD11G1147600.v1.1
prunus_persica	Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0056931
rosa_laevigata	RLG00000035140
rosa_multiflora	Rmu_sc0002035.1_g000008
rosa_roxburghii	Rroxscaffold_1G00023260 Rroxscaffold_1G00059860
rosa_rugosa	Rorug05G0304400
rosa_samantha	Rh5AG373800 Rh5BG384800 Rh5CG408100
rosa_wichuraiana	Rw5G035180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	851
Acc36I	ACCTGC	1 cut(s)	2308
Acc65I	GGTACC	1 cut(s)	1912
AccB1I	GGYRCC	3 cut(s)	377, 388, 1912
AccBSI	CCGCTC	2 cut(s)	66, 433
AccI	GTMKAC	1 cut(s)	1636
AccII	CGCG	5 cut(s)	120, 174, 266, 326, 413
AclWI	GGATC	1 cut(s)	991
AcoI	YGGCCR	4 cut(s)	321, 417, 672, 1933
AcsI	RAATTY	3 cut(s)	1107, 1527, 1707
AcuI	CTGAAG	3 cut(s)	992, 1101, 2058
AcyI	GRCGYC	1 cut(s)	378
AfaI	GTAC	4 cut(s)	1154, 1545, 1914, 1948
AfeI	AGCGCT	1 cut(s)	785
AfiI	CCNNNNNNNGG	5 cut(s)	325, 907, 952, 1280, 1936
AflIII	ACRYGT	1 cut(s)	1226
AhdI	GACNNNNNGTC	1 cut(s)	2196
AjiI	CACGTC	1 cut(s)	2264
AjnI	CCWGG	2 cut(s)	927, 1539
Alw26I	GTCTC	1 cut(s)	1119
AlwI	GGATC	1 cut(s)	991
Ama87I	CYCGRG	1 cut(s)	1554
Aor51HI	AGCGCT	1 cut(s)	785
AoxI	GGCC	5 cut(s)	321, 417, 477, 672, 1933
ApeKI	GCWGC	8 cut(s)	308, 968, 1052, 1067, 1130, 1922, 2180, 2213
ApoI	RAATTY	3 cut(s)	1107, 1527, 1707
Asp700I	GAANNNNTTC	2 cut(s)	528, 2001
Asp718I	GGTACC	1 cut(s)	1912
AspS9I	GGNCC	2 cut(s)	478, 1256
AsuC2I	CCSGG	3 cut(s)	320, 656, 1937
AsuHPI	GGTGA	2 cut(s)	2185, 2354
AsuII	TTCGAA	1 cut(s)	1841
AvaI	CYCGRG	1 cut(s)	1554
AvaII	GGWCC	1 cut(s)	1256
BaeGI	GKGCMC	1 cut(s)	1357
BanI	GGYRCC	3 cut(s)	377, 388, 1912
BanII	GRGCYC	2 cut(s)	320, 1561
BarI	GAAGNNNNNNTAC	2 cut(s)	1862, 1894
BbsI	GAAGAC	2 cut(s)	35, 309
BbvI	GCAGC	8 cut(s)	295, 955, 1039, 1054, 1117, 1909, 2167, 2200
BccI	CCATC	3 cut(s)	496, 1337, 1370
BceAI	ACGGC	4 cut(s)	411, 453, 813, 1760
BciT130I	CCWGG	2 cut(s)	929, 1541
BciVI	GTATCC	1 cut(s)	1256
BcnI	CCSGG	3 cut(s)	320, 656, 1937
BcoDI	GTCTC	1 cut(s)	1119
BfaI	CTAG	2 cut(s)	252, 2389
BfmI	CTRYAG	3 cut(s)	1068, 2104, 2214
BfoI	RGCGCY	3 cut(s)	381, 743, 787
BfuAI	ACCTGC	1 cut(s)	2308
BfuI	GTATCC	1 cut(s)	1256
BglI	GCCNNNNNGGC	1 cut(s)	386
Bme1390I	CCNGG	5 cut(s)	320, 656, 929, 1541, 1937
Bme18I	GGWCC	1 cut(s)	1256
BmeRI	GACNNNNNGTC	1 cut(s)	2196
BmeT110I	CYCGRG	1 cut(s)	1554
BmgBI	CACGTC	1 cut(s)	2264
BmgT120I	GGNCC	2 cut(s)	478, 1256
BmiI	GGNNCC	4 cut(s)	379, 390, 531, 1914
BmrFI	CCNGG	5 cut(s)	320, 656, 929, 1541, 1937
BmrI	ACTGGG	2 cut(s)	1067, 1244
BmsI	GCATC	4 cut(s)	781, 837, 1759, 1813
BmuI	ACTGGG	2 cut(s)	1067, 1244
BpiI	GAAGAC	2 cut(s)	35, 309
BpmI	CTGGAG	1 cut(s)	2121
Bpu10I	CCTNAGC	3 cut(s)	617, 858, 906
Bpu14I	TTCGAA	1 cut(s)	1841
BpuEI	CTTGAG	1 cut(s)	1632
BpuMI	CCSGG	3 cut(s)	320, 656, 1937
BsaHI	GRCGYC	1 cut(s)	378
BsaJI	CCNNGG	4 cut(s)	270, 324, 1930, 1936
BsaWI	WCCGGW	1 cut(s)	1418
Bsc4I	CCNNNNNNNGG	5 cut(s)	325, 907, 952, 1280, 1936
Bse1I	ACTGG	6 cut(s)	364, 1062, 1250, 1862, 1908, 2032
Bse3DI	GCAATG	1 cut(s)	379
BseBI	CCWGG	2 cut(s)	929, 1541
BseDI	CCNNGG	4 cut(s)	270, 324, 1930, 1936
BseGI	GGATG	3 cut(s)	833, 1182, 1381
BseLI	CCNNNNNNNGG	5 cut(s)	325, 907, 952, 1280, 1936
BseMI	GCAATG	1 cut(s)	379
BseMII	CTCAG	6 cut(s)	849, 1187, 1281, 1782, 2174, 2304
BseNI	ACTGG	6 cut(s)	364, 1062, 1250, 1862, 1908, 2032
BseRI	GAGGAG	3 cut(s)	70, 443, 775
BseSI	GKGCMC	1 cut(s)	1357
BseX3I	CGGCCG	2 cut(s)	321, 417
BseXI	GCAGC	8 cut(s)	295, 955, 1039, 1054, 1117, 1909, 2167, 2200
BseYI	CCCAGC	1 cut(s)	245
BsgI	GTGCAG	1 cut(s)	1038
Bsh1236I	CGCG	5 cut(s)	120, 174, 266, 326, 413
Bsh1285I	CGRYCG	2 cut(s)	324, 420
BshFI	GGCC	5 cut(s)	323, 419, 479, 674, 1935
BshNI	GGYRCC	3 cut(s)	377, 388, 1912
BsiEI	CGRYCG	2 cut(s)	324, 420
BsiHKCI	CYCGRG	1 cut(s)	1554
BsiSI	CCGG	8 cut(s)	165, 320, 420, 641, 656, 689, 1419, 1936
BslFI	GGGAC	5 cut(s)	527, 734, 815, 1074, 1910
BslI	CCNNNNNNNGG	5 cut(s)	325, 907, 952, 1280, 1936
BsmAI	GTCTC	1 cut(s)	1119
BsmFI	GGGAC	5 cut(s)	527, 734, 815, 1074, 1910
BsnI	GGCC	5 cut(s)	323, 419, 479, 674, 1935
BsoBI	CYCGRG	1 cut(s)	1554
Bsp119I	TTCGAA	1 cut(s)	1841
Bsp1286I	GDGCHC	3 cut(s)	320, 1357, 1561
Bsp143I	GATC	2 cut(s)	996, 1179
Bsp19I	CCATGG	1 cut(s)	1930
BspANI	GGCC	5 cut(s)	323, 419, 479, 674, 1935
BspCNI	CTCAG	6 cut(s)	850, 1188, 1282, 1783, 2173, 2305
BspFNI	CGCG	5 cut(s)	120, 174, 266, 326, 413
BspLI	GGNNCC	4 cut(s)	379, 390, 531, 1914
BspMAI	CTGCAG	2 cut(s)	1072, 2218
BspMI	ACCTGC	1 cut(s)	2308
BspPI	GGATC	1 cut(s)	991
BspQI	GCTCTTC	1 cut(s)	2046
BspT104I	TTCGAA	1 cut(s)	1841
BspT107I	GGYRCC	3 cut(s)	377, 388, 1912
BsrBI	CCGCTC	2 cut(s)	66, 433
BsrDI	GCAATG	1 cut(s)	379
BsrI	ACTGG	6 cut(s)	364, 1062, 1250, 1862, 1908, 2032
BssECI	CCNNGG	4 cut(s)	270, 324, 1930, 1936
BssMI	GATC	2 cut(s)	996, 1179
BssNI	GRCGYC	1 cut(s)	378
BssT1I	CCWWGG	2 cut(s)	270, 1930
Bst2UI	CCWGG	2 cut(s)	929, 1541
Bst4CI	ACNGT	6 cut(s)	625, 871, 980, 1397, 1514, 2080
Bst6I	CTCTTC	8 cut(s)	92, 211, 217, 730, 1119, 1992, 2046, 2256
BstACI	GRCGYC	1 cut(s)	378
BstBI	TTCGAA	1 cut(s)	1841
BstC8I	GCNNGC	9 cut(s)	172, 387, 750, 854, 1266, 1353, 1978, 2065, 2069
BstDSI	CCRYGG	2 cut(s)	324, 1930
BstENI	CCTNNNNNAGG	1 cut(s)	905
BstF5I	GGATG	3 cut(s)	833, 1182, 1381
BstFNI	CGCG	5 cut(s)	120, 174, 266, 326, 413
BstH2I	RGCGCY	3 cut(s)	381, 743, 787
BstKTI	GATC	2 cut(s)	999, 1182
BstMAI	GTCTC	1 cut(s)	1119
BstMBI	GATC	2 cut(s)	996, 1179
BstMCI	CGRYCG	2 cut(s)	324, 420
BstNI	CCWGG	2 cut(s)	929, 1541
BstSCI	CCNGG	5 cut(s)	318, 654, 927, 1539, 1935
BstSFI	CTRYAG	3 cut(s)	1068, 2104, 2214
BstSLI	GKGCMC	1 cut(s)	1357
BstUI	CGCG	5 cut(s)	120, 174, 266, 326, 413
BstV1I	GCAGC	8 cut(s)	295, 955, 1039, 1054, 1117, 1909, 2167, 2200
BstV2I	GAAGAC	2 cut(s)	35, 309
BstXI	CCANNNNNNTGG	1 cut(s)	2145
BstZI	CGGCCG	2 cut(s)	321, 417
BsuI	GTATCC	1 cut(s)	1256
BsuRI	GGCC	5 cut(s)	323, 419, 479, 674, 1935
BtgI	CCRYGG	2 cut(s)	324, 1930
BtrI	CACGTC	1 cut(s)	2264
BtsCI	GGATG	3 cut(s)	833, 1182, 1381
BtsI	GCAGTG	3 cut(s)	20, 1452, 2049
BtsIMutI	CAGTG	8 cut(s)	20, 972, 1055, 1452, 1901, 2025, 2049, 2085
BveI	ACCTGC	1 cut(s)	2308
Cac8I	GCNNGC	9 cut(s)	172, 387, 750, 854, 1266, 1353, 1978, 2065, 2069
Cfr13I	GGNCC	2 cut(s)	478, 1256
Cfr42I	CCGCGG	1 cut(s)	327
CseI	GACGC	2 cut(s)	161, 314
Csp6I	GTAC	4 cut(s)	1153, 1544, 1913, 1947
CviAII	CATG	7 cut(s)	124, 160, 695, 1369, 1576, 1882, 1931
CviQI	GTAC	4 cut(s)	1153, 1544, 1913, 1947
DinI	GGCGCC	1 cut(s)	379
DpnI	GATC	2 cut(s)	998, 1181
DpnII	GATC	2 cut(s)	996, 1179
DriI	GACNNNNNGTC	1 cut(s)	2196
EaeI	YGGCCR	4 cut(s)	321, 417, 672, 1933
EagI	CGGCCG	2 cut(s)	321, 417
Eam1104I	CTCTTC	8 cut(s)	92, 211, 217, 730, 1119, 1992, 2046, 2256
Eam1105I	GACNNNNNGTC	1 cut(s)	2196
EarI	CTCTTC	8 cut(s)	92, 211, 217, 730, 1119, 1992, 2046, 2256
EciI	GGCGGA	2 cut(s)	133, 598
EclXI	CGGCCG	2 cut(s)	321, 417
Eco130I	CCWWGG	2 cut(s)	270, 1930
Eco24I	GRGCYC	2 cut(s)	320, 1561
Eco47I	GGWCC	1 cut(s)	1256
Eco47III	AGCGCT	1 cut(s)	785
Eco52I	CGGCCG	2 cut(s)	321, 417
Eco57I	CTGAAG	3 cut(s)	992, 1101, 2058
Eco88I	CYCGRG	1 cut(s)	1554
EcoNI	CCTNNNNNAGG	1 cut(s)	905
EcoO109I	RGGNCCY	1 cut(s)	478
EcoRII	CCWGG	2 cut(s)	927, 1539
EcoT14I	CCWWGG	2 cut(s)	270, 1930
EcoT38I	GRGCYC	2 cut(s)	320, 1561
EgeI	GGCGCC	1 cut(s)	379
EheI	GGCGCC	1 cut(s)	379
ErhI	CCWWGG	2 cut(s)	270, 1930
FaeI	CATG	7 cut(s)	127, 163, 698, 1372, 1579, 1885, 1934
FalI	AAGNNNNNCTT	2 cut(s)	2031, 2063
FaqI	GGGAC	5 cut(s)	527, 734, 815, 1074, 1910
FatI	CATG	7 cut(s)	123, 159, 694, 1368, 1575, 1881, 1930
FauI	CCCGC	1 cut(s)	442
FauNDI	CATATG	2 cut(s)	948, 2012
FblI	GTMKAC	1 cut(s)	1636
FokI	GGATG	3 cut(s)	820, 1169, 1388
FriOI	GRGCYC	2 cut(s)	320, 1561
FspBI	CTAG	2 cut(s)	252, 2389
FspI	TGCGCA	1 cut(s)	851
GsaI	CCCAGC	1 cut(s)	249
GsuI	CTGGAG	1 cut(s)	2121
HaeII	RGCGCY	3 cut(s)	381, 743, 787
HaeIII	GGCC	5 cut(s)	323, 419, 479, 674, 1935
HapII	CCGG	8 cut(s)	165, 320, 420, 641, 656, 689, 1419, 1936
HgaI	GACGC	2 cut(s)	161, 314
Hin1I	GRCGYC	1 cut(s)	378
Hin1II	CATG	7 cut(s)	127, 163, 698, 1372, 1579, 1885, 1934
HincII	GTYRAC	1 cut(s)	1637
HindII	GTYRAC	1 cut(s)	1637
HindIII	AAGCTT	1 cut(s)	2301
HinfI	GANTC	9 cut(s)	329, 575, 722, 911, 1042, 1213, 1685, 1898, 2231
HpaII	CCGG	8 cut(s)	165, 320, 420, 641, 656, 689, 1419, 1936
HphI	GGTGA	2 cut(s)	2185, 2354
Hpy166II	GTNNAC	4 cut(s)	983, 1153, 1171, 1637
Hpy188I	TCNGA	7 cut(s)	99, 315, 1197, 1291, 1792, 2038, 2314
Hpy188III	TCNNGA	6 cut(s)	359, 1103, 1310, 1649, 1766, 2138
Hpy8I	GTNNAC	4 cut(s)	983, 1153, 1171, 1637
Hpy99I	CGWCG	2 cut(s)	155, 397
HpyAV	CCTTC	9 cut(s)	157, 347, 469, 791, 913, 946, 1202, 1546, 1848
HpyCH4III	ACNGT	6 cut(s)	625, 871, 980, 1397, 1514, 2080
HpyCH4IV	ACGT	4 cut(s)	774, 1226, 1945, 2263
HpySE526I	ACGT	4 cut(s)	774, 1226, 1945, 2263
Hsp92I	GRCGYC	1 cut(s)	378
Hsp92II	CATG	7 cut(s)	127, 163, 698, 1372, 1579, 1885, 1934
KasI	GGCGCC	1 cut(s)	377
KpnI	GGTACC	1 cut(s)	1916
KspI	CCGCGG	1 cut(s)	327
Kzo9I	GATC	2 cut(s)	996, 1179
LguI	GCTCTTC	1 cut(s)	2046
LmnI	GCTCC	6 cut(s)	260, 316, 430, 657, 691, 706
Lsp1109I	GCAGC	8 cut(s)	295, 955, 1039, 1054, 1117, 1909, 2167, 2200
LweI	GCATC	4 cut(s)	781, 837, 1759, 1813
MaeI	CTAG	2 cut(s)	252, 2389
MaeII	ACGT	4 cut(s)	774, 1226, 1945, 2263
MaeIII	GTNAC	2 cut(s)	74, 1848
MalI	GATC	2 cut(s)	998, 1181
MbiI	CCGCTC	2 cut(s)	66, 433
MboI	GATC	2 cut(s)	996, 1179
MfeI	CAATTG	2 cut(s)	284, 443
MhlI	GDGCHC	3 cut(s)	320, 1357, 1561
Mly113I	GGCGCC	1 cut(s)	378
MlyI	GAGTC	5 cut(s)	584, 716, 1036, 1679, 1892
MmeI	TCCRAC	2 cut(s)	1234, 2370
MroXI	GAANNNNTTC	2 cut(s)	528, 2001
MseI	TTAA	9 cut(s)	777, 1143, 1457, 1587, 1629, 1779, 1799, 1814, 1854
MslI	CAYNNNNRTG	1 cut(s)	2367
MspA1I	CMGCKG	1 cut(s)	326
MspI	CCGG	8 cut(s)	165, 320, 420, 641, 656, 689, 1419, 1936
MspR9I	CCNGG	5 cut(s)	320, 656, 929, 1541, 1937
MunI	CAATTG	2 cut(s)	284, 443
MvaI	CCWGG	2 cut(s)	929, 1541
MvnI	CGCG	5 cut(s)	120, 174, 266, 326, 413
NarI	GGCGCC	1 cut(s)	378
NciI	CCSGG	3 cut(s)	320, 656, 1937
NcoI	CCATGG	1 cut(s)	1930
NdeI	CATATG	2 cut(s)	948, 2012
NdeII	GATC	2 cut(s)	996, 1179
NlaIII	CATG	7 cut(s)	127, 163, 698, 1372, 1579, 1885, 1934
NlaIV	GGNNCC	4 cut(s)	379, 390, 531, 1914
NmeAIII	GCCGAG	2 cut(s)	406, 650
NmuCI	GTSAC	2 cut(s)	74, 1848
NsbI	TGCGCA	1 cut(s)	851
NspV	TTCGAA	1 cut(s)	1841
PciSI	GCTCTTC	1 cut(s)	2046
PcsI	WCGNNNNNNNCGW	2 cut(s)	2260, 2269
PdmI	GAANNNNTTC	2 cut(s)	528, 2001
PfeI	GAWTC	4 cut(s)	329, 911, 1213, 2231
PleI	GAGTC	5 cut(s)	583, 716, 1036, 1679, 1892
PluTI	GGCGCC	1 cut(s)	381
PpsI	GAGTC	5 cut(s)	583, 716, 1036, 1679, 1892
Psp6I	CCWGG	2 cut(s)	927, 1539
PspFI	CCCAGC	1 cut(s)	245
PspGI	CCWGG	2 cut(s)	927, 1539
PspN4I	GGNNCC	4 cut(s)	379, 390, 531, 1914
PspPI	GGNCC	2 cut(s)	478, 1256
PstI	CTGCAG	2 cut(s)	1072, 2218
RsaI	GTAC	4 cut(s)	1154, 1545, 1914, 1948
RsaNI	GTAC	4 cut(s)	1153, 1544, 1913, 1947
RseI	CAYNNNNRTG	1 cut(s)	2367
SacII	CCGCGG	1 cut(s)	327
SalI	GTCGAC	1 cut(s)	1635
SapI	GCTCTTC	1 cut(s)	2046
SaqAI	TTAA	9 cut(s)	777, 1143, 1457, 1587, 1629, 1779, 1799, 1814, 1854
Sau3AI	GATC	2 cut(s)	996, 1179
Sau96I	GGNCC	2 cut(s)	478, 1256
SchI	GAGTC	5 cut(s)	584, 716, 1036, 1679, 1892
ScrFI	CCNGG	5 cut(s)	320, 656, 929, 1541, 1937
SduI	GDGCHC	3 cut(s)	320, 1357, 1561
SfaNI	GCATC	4 cut(s)	781, 837, 1759, 1813
SfcI	CTRYAG	3 cut(s)	1068, 2104, 2214
SfoI	GGCGCC	1 cut(s)	379
Sfr303I	CCGCGG	1 cut(s)	327
SfuI	TTCGAA	1 cut(s)	1841
SgrBI	CCGCGG	1 cut(s)	327
SinI	GGWCC	1 cut(s)	1256
SmiMI	CAYNNNNRTG	1 cut(s)	2367
SmlI	CTYRAG	1 cut(s)	1647
SmoI	CTYRAG	1 cut(s)	1647
SspDI	GGCGCC	1 cut(s)	377
SspMI	CTAG	2 cut(s)	252, 2389
StyD4I	CCNGG	5 cut(s)	318, 654, 927, 1539, 1935
StyI	CCWWGG	2 cut(s)	270, 1930
TaaI	ACNGT	6 cut(s)	625, 871, 980, 1397, 1514, 2080
TaiI	ACGT	4 cut(s)	777, 1229, 1948, 2266
TaqI	TCGA	6 cut(s)	150, 1013, 1399, 1636, 1841, 2254
TatI	WGTACW	1 cut(s)	1152
TauI	GCSGC	7 cut(s)	179, 182, 185, 326, 416, 419, 1775
TfiI	GAWTC	4 cut(s)	329, 911, 1213, 2231
Tru1I	TTAA	9 cut(s)	777, 1143, 1457, 1587, 1629, 1779, 1799, 1814, 1854
Tru9I	TTAA	9 cut(s)	777, 1143, 1457, 1587, 1629, 1779, 1799, 1814, 1854
TscAI	CASTG	8 cut(s)	27, 979, 1062, 1452, 1908, 2032, 2049, 2085
TseFI	GTSAC	2 cut(s)	74, 1848
TseI	GCWGC	8 cut(s)	308, 968, 1052, 1067, 1130, 1922, 2180, 2213
Tsp45I	GTSAC	2 cut(s)	74, 1848
TspDTI	ATGAA	5 cut(s)	822, 965, 1764, 2010, 2295
TspGWI	ACGGA	4 cut(s)	62, 522, 611, 989
TspRI	CASTG	8 cut(s)	27, 979, 1062, 1452, 1908, 2032, 2049, 2085
VpaK11BI	GGWCC	1 cut(s)	1256
XagI	CCTNNNNNAGG	1 cut(s)	905
XapI	RAATTY	3 cut(s)	1107, 1527, 1707
XcmI	CCANNNNNNNNNTGG	2 cut(s)	340, 371
XmiI	GTMKAC	1 cut(s)	1636
XmnI	GAANNNNTTC	2 cut(s)	528, 2001
XspI	CTAG	2 cut(s)	252, 2389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.