RchiOBHm_Chr4g0399301

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016126
Physical Location & Seq
Forward (+)
16470832 .. 16474170
3339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGGACTCTCTGCTAGCAAACTACGCCTCTTCCGACGAAGAAGAAGATCAAAAACCCCACCACCACCACCAACTACAACCACCTCCAAACCCTTCTTCTTCTTCTTCTTCTTCTCTTTTCTCTTCTCTACCCAAACCCAAACCCGAACCCGAACCTTCATCCTTCTTTAATTCCCTTCCTCCTCCGAAACAAACCCATTCAAATCCTCCCCACCAAGAAGACCAAGACTCAAAACCCACCTCAAGATCTTCTCTTTTCTCCTCTCTACCCAAACCCAAACCCTCCTCTCTCTTCCAATCCCTTCCCAAACCAAAACTTGAACCACAAGAAGAAGAAGAAGAAGACATAAAACCCCCTTCTTCTGTTTTCTCTTCTCTTCCTCCCCCCAAATCCCAAATCCCTAACAAACCCATCTCCAATTCCTCATCTTCTCCTGCCCAAATCCCCAAAAGGGTCGTCCAATTCAAGCCTCCCATCAACCCCTATTTCGCAAACTCTTCCCATCTCGAAGACGACGACGACGATGAGGAAGAAGAAGAGAGGCGGCGGCGAAAAGCCTCGGAGGCCTCGTCGGCGCAACCTTTGTCCGTGACGTCCTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAATTCCGGTTTAGGTTCCGGTGTAGGTTCCGGTAGGAGAGCCACCGTTGAAACTGAGTCGTGTGGTTCTAAAGTGGAGAGCGATGTGGGTTTGGATCGAAATGAAAATGTAGCAAGTTATGATGATCCCCAATCAAATTTTGATCCAAATGCAGAAGGTTATGGTGGGTATGAGAGTTACCAATATGGTGTTGACCAAAATGTGGATTCTGGGGTTAGTGGCGGTGATGGTTCGAGCTATGGTAGTTACAGTGGTTATGGTGGGTATAATGGTTATGCTGGAAATCATGGGAACGAATGGGCTGATGGGTCGGGGACTGCGGCGGCAATGGGTGTGTCAGGTGATACTGGGGTTAAAGTGAGTGGGAAGAGGAGGAGGAATGATGTCCCAACTGAAATACTTGAGGTTAAGCAGGATGAGTTGATGAAGAATCGGCCGAGGGAGGACCAAGCCAAGGTGACCGGGATAGCTTTTGGGCCTTCTTACCAGCCTGTTTCGACGAAGGGGAAACCGACAAAGCTGCATAAGAGGAAGCATCAGATTGGTTCTCTGTACTTTGATATGAAGCAGAAGGAGATGGAACTTCAGGAGCGGCGTTCAAGAGGCTTCCTTACAAAAGCTGAAACACAAGCCAAGTATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

427

Amino Acids

46.49

Weight (kDa)

5.6

Isoelectric Point (pI)

66.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19270
fragaria_vesca FvH4_4g07150
malus_domestica MD13G1239600.v1.1
prunus_persica Prupe.1G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399301
rosa_laevigata RLG00000009275
rosa_multiflora Rmu_sc0003135.1_g000009 Rmu_sc0016554.1_g000003
rosa_rugosa Rorug04G0016400
rosa_samantha Rh4AG094100 Rh4BG091500 Rh4CG102300 Rh4DG086200 Rh7DG334700
rosa_wichuraiana Rw4G007720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 838
AccBSI CCGCTC 1 cut(s) 1228
AciI CCGC 6 cut(s) 544, 547, 858, 956, 959, 1228
AclWI GGATC 3 cut(s) 738, 755, 773
AcoI YGGCCR 1 cut(s) 1070
AcsI RAATTY 2 cut(s) 637, 772
AcuI CTGAAG 1 cut(s) 1205
AcyI GRCGYC 2 cut(s) 593, 617
AfaI GTAC 1 cut(s) 1190
AfiI CCNNNNNNNGG 3 cut(s) 451, 838, 1090
AgsI TTSAA 5 cut(s) 201, 320, 466, 686, 1236
AjnI CCWGG 1 cut(s) 620
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AluBI AGCT 5 cut(s) 608, 873, 1106, 1156, 1256
AluI AGCT 5 cut(s) 608, 873, 1106, 1156, 1256
AlwI GGATC 3 cut(s) 738, 755, 773
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 3 cut(s) 564, 1070, 1112
ApeKI GCWGC 1 cut(s) 1156
ApoI RAATTY 2 cut(s) 637, 772
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AspLEI GCGC 2 cut(s) 577, 619
AspS9I GGNCC 2 cut(s) 1081, 1112
AsuC2I CCSGG 1 cut(s) 1099
AsuHPI GGTGA 3 cut(s) 872, 989, 1105
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 628
AvaII GGWCC 1 cut(s) 1081
BaeI ACNNNNGTAYC 2 cut(s) 972, 1005
BanI GGYRCC 1 cut(s) 616
BbsI GAAGAC 3 cut(s) 225, 348, 516
BbvI GCAGC 1 cut(s) 1143
BccI CCATC 7 cut(s) 419, 482, 510, 857, 935, 1207, 1272
BcgI CGANNNNNNTGC 2 cut(s) 1138, 1172
BciT130I CCWGG 1 cut(s) 622
BcnI CCSGG 1 cut(s) 1099
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 1 cut(s) 620
BglI GCCNNNNNGGC 2 cut(s) 563, 572
BglII AGATCT 1 cut(s) 245
BisI GCNGC 6 cut(s) 545, 548, 957, 960, 1157, 1229
BlsI GCNGC 6 cut(s) 546, 549, 958, 961, 1158, 1230
Bme1390I CCNGG 2 cut(s) 622, 1099
Bme18I GGWCC 1 cut(s) 1081
BmeT110I CYCGRG 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 1081, 1112
BmiI GGNNCC 3 cut(s) 618, 652, 664
BmrFI CCNGG 2 cut(s) 622, 1099
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 1 cut(s) 1180
BmtI GCTAGC 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 3 cut(s) 225, 348, 516
BpuEI CTTGAG 2 cut(s) 226, 1058
BpuMI CCSGG 1 cut(s) 1099
BsaHI GRCGYC 2 cut(s) 593, 617
BsaJI CCNNGG 4 cut(s) 558, 620, 1073, 1089
BsaWI WCCGGW 3 cut(s) 641, 653, 665
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 3 cut(s) 451, 838, 1090
Bse1I ACTGG 1 cut(s) 988
Bse3DI GCAATG 1 cut(s) 969
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 4 cut(s) 558, 620, 1073, 1089
BseGI GGATG 3 cut(s) 158, 1057, 1283
BseLI CCNNNNNNNGG 3 cut(s) 451, 838, 1090
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 5 cut(s) 171, 250, 274, 1021, 1024
BseX3I CGGCCG 1 cut(s) 1070
BseXI GCAGC 1 cut(s) 1143
Bsh1285I CGRYCG 1 cut(s) 1073
BshFI GGCC 3 cut(s) 566, 1072, 1114
BshNI GGYRCC 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 1073
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 5 cut(s) 614, 642, 654, 666, 1098
BslFI GGGAC 2 cut(s) 964, 1007
BslI CCNNNNNNNGG 3 cut(s) 451, 838, 1090
BsmFI GGGAC 2 cut(s) 964, 1007
BsnI GGCC 3 cut(s) 566, 1072, 1114
BsoBI CYCGRG 1 cut(s) 628
Bsp143I GATC 5 cut(s) 46, 245, 730, 760, 778
BspACI CCGC 6 cut(s) 544, 547, 858, 956, 959, 1228
BspANI GGCC 3 cut(s) 566, 1072, 1114
BspCNI CTCAG 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 618, 652, 664
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 3 cut(s) 738, 755, 773
BspT107I GGYRCC 1 cut(s) 616
BsrBI CCGCTC 1 cut(s) 1228
BsrDI GCAATG 1 cut(s) 969
BsrI ACTGG 1 cut(s) 988
BssECI CCNNGG 4 cut(s) 558, 620, 1073, 1089
BssMI GATC 5 cut(s) 46, 245, 730, 760, 778
BssNI GRCGYC 2 cut(s) 593, 617
BssT1I CCWWGG 1 cut(s) 1089
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 2 cut(s) 682, 887
Bst6I CTCTTC 8 cut(s) 34, 127, 296, 376, 381, 502, 531, 998
BstACI GRCGYC 2 cut(s) 593, 617
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 690
BstEII GGTNACC 1 cut(s) 1093
BstF5I GGATG 3 cut(s) 158, 1057, 1283
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 2 cut(s) 577, 619
BstKTI GATC 5 cut(s) 49, 248, 733, 763, 781
BstMBI GATC 5 cut(s) 46, 245, 730, 760, 778
BstMCI CGRYCG 1 cut(s) 1073
BstMWI GCNNNNNNNGC 4 cut(s) 23, 563, 572, 614
BstNI CCWGG 1 cut(s) 622
BstPI GGTNACC 1 cut(s) 1093
BstSCI CCNGG 2 cut(s) 620, 1097
BstV1I GCAGC 1 cut(s) 1143
BstV2I GAAGAC 3 cut(s) 225, 348, 516
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BstZI CGGCCG 1 cut(s) 1070
BsuRI GGCC 3 cut(s) 566, 1072, 1114
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 3 cut(s) 158, 1057, 1283
BtsIMutI CAGTG 1 cut(s) 892
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 577, 619
Cfr13I GGNCC 2 cut(s) 1081, 1112
Csp6I GTAC 1 cut(s) 1189
CviAII CATG 1 cut(s) 923
CviQI GTAC 1 cut(s) 1189
DdeI CTNAG 1 cut(s) 690
DinI GGCGCC 1 cut(s) 618
DpnI GATC 5 cut(s) 48, 247, 732, 762, 780
DpnII GATC 5 cut(s) 46, 245, 730, 760, 778
EaeI YGGCCR 1 cut(s) 1070
EagI CGGCCG 1 cut(s) 1070
Eam1104I CTCTTC 8 cut(s) 34, 127, 296, 376, 381, 502, 531, 998
EarI CTCTTC 8 cut(s) 34, 127, 296, 376, 381, 502, 531, 998
EclXI CGGCCG 1 cut(s) 1070
Eco130I CCWWGG 1 cut(s) 1089
Eco147I AGGCCT 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 1081
Eco52I CGGCCG 1 cut(s) 1070
Eco57I CTGAAG 1 cut(s) 1205
Eco88I CYCGRG 1 cut(s) 628
Eco91I GGTNACC 1 cut(s) 1093
EcoO65I GGTNACC 1 cut(s) 1093
EcoRI GAATTC 1 cut(s) 637
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 1089
EgeI GGCGCC 1 cut(s) 618
EheI GGCGCC 1 cut(s) 618
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 1 cut(s) 926
FaqI GGGAC 2 cut(s) 964, 1007
FatI CATG 1 cut(s) 922
Fnu4HI GCNGC 6 cut(s) 545, 548, 957, 960, 1157, 1229
FokI GGATG 2 cut(s) 145, 1064
Fsp4HI GCNGC 6 cut(s) 545, 548, 957, 960, 1157, 1229
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 576, 618
GluI GCNGC 6 cut(s) 545, 548, 957, 960, 1157, 1229
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 3 cut(s) 566, 1072, 1114
HapII CCGG 5 cut(s) 614, 642, 654, 666, 1098
HhaI GCGC 2 cut(s) 577, 619
Hin1I GRCGYC 2 cut(s) 593, 617
Hin1II CATG 1 cut(s) 926
Hin6I GCGC 2 cut(s) 575, 617
HinP1I GCGC 2 cut(s) 575, 617
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HinfI GANTC 5 cut(s) 5, 227, 692, 842, 1066
HpaII CCGG 5 cut(s) 614, 642, 654, 666, 1098
HphI GGTGA 3 cut(s) 872, 989, 1105
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 4 cut(s) 34, 186, 562, 1176
Hpy188III TCNNGA 4 cut(s) 243, 506, 1223, 1236
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 6 cut(s) 38, 518, 521, 524, 574, 1138
HpyCH4III ACNGT 2 cut(s) 682, 887
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 2 cut(s) 788, 1159
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 563, 572, 614
HpyF3I CTNAG 1 cut(s) 690
HpySE526I ACGT 1 cut(s) 593
Hsp92I GRCGYC 2 cut(s) 593, 617
Hsp92II CATG 1 cut(s) 926
HspAI GCGC 2 cut(s) 575, 617
KasI GGCGCC 1 cut(s) 616
Kzo9I GATC 5 cut(s) 46, 245, 730, 760, 778
LmnI GCTCC 1 cut(s) 1225
Lsp1109I GCAGC 1 cut(s) 1143
LweI GCATC 1 cut(s) 1180
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 4 cut(s) 589, 812, 881, 1093
MalI GATC 5 cut(s) 48, 247, 732, 762, 780
MbiI CCGCTC 1 cut(s) 1228
MboI GATC 5 cut(s) 46, 245, 730, 760, 778
MflI RGATCY 1 cut(s) 245
MluCI AATT 5 cut(s) 169, 418, 461, 637, 772
Mly113I GGCGCC 1 cut(s) 617
MlyI GAGTC 2 cut(s) 221, 701
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 3 cut(s) 168, 990, 1044
MspI CCGG 5 cut(s) 614, 642, 654, 666, 1098
MspR9I CCNGG 2 cut(s) 622, 1099
MvaI CCWGG 1 cut(s) 622
MwoI GCNNNNNNNGC 4 cut(s) 23, 563, 572, 614
NarI GGCGCC 1 cut(s) 617
NciI CCSGG 1 cut(s) 1099
NdeII GATC 5 cut(s) 46, 245, 730, 760, 778
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 926
NlaIV GGNNCC 3 cut(s) 618, 652, 664
NmeAIII GCCGAG 1 cut(s) 1098
NmuCI GTSAC 2 cut(s) 589, 1093
PceI AGGCCT 1 cut(s) 566
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 513, 519, 566
PfeI GAWTC 2 cut(s) 842, 1066
PflMI CCANNNNNTGG 1 cut(s) 838
PkrI GCNGC 6 cut(s) 546, 549, 958, 961, 1158, 1230
PleI GAGTC 2 cut(s) 221, 700
PluTI GGCGCC 1 cut(s) 620
PpsI GAGTC 2 cut(s) 221, 700
Psp6I CCWGG 1 cut(s) 620
PspEI GGTNACC 1 cut(s) 1093
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 3 cut(s) 618, 652, 664
PspPI GGNCC 2 cut(s) 1081, 1112
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 1 cut(s) 1190
RsaNI GTAC 1 cut(s) 1189
SaqAI TTAA 3 cut(s) 168, 990, 1044
SatI GCNGC 6 cut(s) 545, 548, 957, 960, 1157, 1229
Sau3AI GATC 5 cut(s) 46, 245, 730, 760, 778
Sau96I GGNCC 2 cut(s) 1081, 1112
SchI GAGTC 2 cut(s) 221, 701
ScrFI CCNGG 2 cut(s) 622, 1099
SfaNI GCATC 1 cut(s) 1180
SfoI GGCGCC 1 cut(s) 618
SinI GGWCC 1 cut(s) 1081
SmlI CTYRAG 2 cut(s) 241, 1037
SmoI CTYRAG 2 cut(s) 241, 1037
Sse9I AATT 5 cut(s) 169, 418, 461, 637, 772
SseBI AGGCCT 1 cut(s) 566
SsiI CCGC 6 cut(s) 544, 547, 858, 956, 959, 1228
SspDI GGCGCC 1 cut(s) 616
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 620, 1097
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 2 cut(s) 682, 887
TaiI ACGT 1 cut(s) 596
TaqI TCGA 4 cut(s) 507, 733, 869, 1133
TasI AATT 5 cut(s) 169, 418, 461, 637, 772
TatI WGTACW 1 cut(s) 1188
TauI GCSGC 5 cut(s) 547, 550, 959, 962, 1231
TfiI GAWTC 2 cut(s) 842, 1066
Tru1I TTAA 3 cut(s) 168, 990, 1044
Tru9I TTAA 3 cut(s) 168, 990, 1044
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 2 cut(s) 589, 1093
TseI GCWGC 1 cut(s) 1156
Tsp45I GTSAC 2 cut(s) 589, 1093
TspDTI ATGAA 4 cut(s) 147, 753, 1076, 1214
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 1 cut(s) 892
Van91I CCANNNNNTGG 1 cut(s) 838
VpaK11BI GGWCC 1 cut(s) 1081
XapI RAATTY 2 cut(s) 637, 772
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.