RchiOBHm_Chr3g0449611

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002633
Physical Location & Seq
Reverse (-)
1324544 .. 1333735
9192 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3672 bp
ATGGGGACAGAACTCATGAGAGTTTGCATAAAGGAAGACAATGATGAATTTCCATCGGTGCCACCGGGATTTGAGTCATTCACATCTTTCAGCTTAAAAAGGGTGAATGAAAGTGAGAAACAAGATAGTGAAAACCTAATCAGTTGCTGTGCCACTACCAGTACTTCTGAATCACAGTCTGTTCAGATGGAAACTGGTAATACTGTTGCTGAAGTTGCAAAGAGGTCTCAACGGCGGAGGCCATGGATAAACAATAGACGGTATTATAACAATGCTGAGGATGAGTTTGATTATGAGAGCCTTGATCAAAATTTTCTTCTAAGGTCGCTTCTTCCTAAAGGGGTTATACGGGGATGTCCAGAGTGCAGTACCTGCCAAAAGGTATCTGCAAGATGGCATCCAGAGGATGGTCAAAGGCCTGACATCCAGGATGCTCCAATATTCAGACCAACTGAAGAGGAGTTCAAAGATACTTTGAAATACATAGCGAGTATACGCCCAAAAGCTGAACCATATGGTATTTGTCGCATTGTTCCTCCCTCTTCATGGAGACCCCCCTGTCCTCTCAAGGAAAAGAATGTTTGGGAGACTTCGAAGTTTGCTACTCGCGTCCAAAGAGTCGACAAACTTCAGAATCGAGATTCAATGAGAAAGATGCCAAAGGGTCATAATCATACGAGGAAGAAAAGGAGAAGATGCACACGAATGGGGGCTGATTGTCCTGTTGGTGGTAGAGGATCTGGGGATGATGGAATTTGTGAGGCAGAAAGGTTTGGGTTTGAACCTGGTCCAGAGTTTACTTTAGGAGCCTTTGAAAAATATGCTGATGACTTCAAGACCCAATACTTTAGCAAGAATGAACACTTTACTGATATAGGAGGTCACTTGAGTGAAGTCAGAGATTGCTGGGAGCCATCTGTGGAAAATATTGAGGGTGAATATTGGCGGATGGTGGAGAAACCTACGGAAGAAATTGAGGTTCTATATGGGGCTGATTTGGAAACAGGAGTTTTCGGAAGTGGTTTTCCCAAGATATACGGTCAGGATGGTTTTACTTCAGAGGAGCAGTATATAACATCTGGCTGGAACTTGAACAACTTTCCTAGACTGCCTGGATCTGTCCTTTCTTACGAAAGCAGTGATATATCTGGTGTTCTGGTGCCATGGTTATATATAGGGATGTGCTTTTCTTCCTTTTGCTGGCATGTTGAAGATCACCACTTGTACTCTTTAAATTACATGCATTGGGGTGCTCCGAAGTTGTGGTATGGTATCCCTGGTAGTGATGCCTGTAAATTTGAAGAGGTTATGAGAAAGCACCTCCCTGACCTATTTGAAGAACAACCTGACTTGCTTCATAAGCTGGTAACACAGCTCTCCCCCTCTATACTGAAATCTGATGGGGTACCAGTGTACAGATGTTGTCAGAATCCTGGAGAGTTTGTCCTGACCTTTCCTCGAGCTTACCATTCAGGGTTCAACTGTGGTTTCAATTGTGCAGAGGCAGTTAACGTTGCTCCTGTTGATTGGTTGCCACATGGACAGGTTGCTATAGAGCTATACCAAGAGCAGGGACGAAAGACATCCATTTCCCATGATAAATTGTTGCTTGGCGCTGCAAGGGAGGCAGTAAGGGCACATTGGGAGCTCAATTTACTGAAGAAGAACACTTCTGATAACTTAAGATGGAAAAATGTCTGTGGAAAGGATGGGATCTTAGCCAAAGTTCTCAAGGCACGTGTTGAGATGGAGCGTGTGAGGAGGGAATTTCTTTGCAATTCTTCCCAGGCATTGAAGATGGAGAGCAATTTTGATGCCACGAGTGAGAGGGAATGCAGTATATGCTTTTTTGATTTGCATCTGTCAGCAGCTGGCTGTCATCAATGTTCCCCAGATAGATATGCATGCTTGAATCATGCAAAGCAATTCTGTTCGTGTGCTTGGAGCACTAAGTTTTTCCTCTTCCGTTATGACATTGATGAATTAAACATCCTTCTCGAAGCACTGGAAGGAAAGTTGAGTGCAGTATACAGATGGGCTAGACTAGATCTTGGATTGGCCTTGAGTTCTTATATTGGCAAAGACAACATAAAAGTTGGTAAATTATCGTATGCCTCCAAGAGTACTATACTTGAAGAAGTGAGCTCACAGCCACAAAGCAATTGCTTTAAAGATCCACCAGGGAAAGAAATCTCTAAAGATGGTCCTGAAAGGTCAACTGGGAGAGAAGAATCATTTCTTAGCACTGCCAACTCTCTGCAGGTTTGCCAGTTGTCTCAAGAAGACACATCATATGCTTTGAACTCGGCTGAAAGGGAATCTGGGATGAAGATGACTTCAGTTGACAATATTATACTTCTTAGTGATGATGAAAGTGATGAGCCAAAGAAGCTACATTCAGACAGCCCTACAGCAATATCTTCTGCTAACCAGTTAGAACTTCCCAATAGATTGGTGGCTTCTGATGGAAAAGTGAGCCCTTGTAATGTTGAGAAAGCTGCAGTCTTAAATATGCCCGTGACTGATGCAGCTGTGATGGTTGAAAAAGTTATTAGTCCGTTGGCTAGTGAAGAAAAAAAGGATTTTTCATCCCATTCTTATTCTCATATTATGAATGTGAAAGATGAACAGGAGAATGATGGACAATCACGATCCAATTCACCTAACCTTTCTTCCAAATTAGCTTCTGCTGGGGCAGAACATGGACCAGACACTTGCCATATTAGTGAACCCAAGGTTGCTATTTCCAGGAGTGACCCGAAAGATTCACAGCCATGTCACAGTATAAAACCTGAGAACGAGGATAGACATGAAAGGATGGGAAGAAATGCTGATGCAAATATAGTGGATAATGTAAGAACTGCAATGGGAAACCCATCATCCAGCCAGAACAATTTGGACAGATATTATCGCCAGAAGGGTCCCCGTATAGCAAAGGTAGTGCGGAGGATCACCTGCATTGTTGAGCCTCTAGAATTTGGAGTCGTGCTCTCTGGAAAGTCATGGTGTAACAGCCAAGCCATTTTTCCGAAAGGATTTAGGAGCCGGGTTAAGTACATTAGTGTTCTCGATCCTACTGTGAGGTGTTATTATGTCTCAGAAGTACTTGATGCAAGACAGGCCGGACCTCTTTTCATGGTTTCTTTGGATCAGTGTCCAGGTGAGGTTTTCATCCATAATTCAGTTGGTAGATGCTGGGAAATGGTGAGAGAGAGAGTCAATCAAGAAATCTCAAGGCACCATAAGTTGGGAAGACCAAACCTTCCTCCTTTGCAGCCACCTGGGAGTCTTGATGGCTTTGAAATGTTTGGTTTTACTTCACCAGTTATTGTACAGGCCATAGAGGCAATGGATAGGAATCGCGTTTGCTCCGAGTACTGGGACTCTCGTCCCTACTCCCGACCTCAAGTGCAGATACCACAAAAAGCTCAATCTGAAGAAAGTGGTGAAAACTTGAATAAGATAACGACGGGAAGGAATGCTCATGAGGCCGCCGGCATTAATCTCTTGACTAGTGGGGTCGATACAATACTCGGAGGCCTGTTCAAGAAGGCTAACCTAGAAGAATTAAACTCACTGTACAGCATCATAAGTGACAATCAGCAGACTGTCGGTCGAGGCCTAGTGACACGACTTCTTAATGAAGAGATTCAAACTCGTCGTCCAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008198 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0040008 GO:0040009 GO:0040010 GO:0040029 GO:0042054 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045814 GO:0045892 GO:0045927 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1223

Amino Acids

137.76

Weight (kDa)

5.94

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 146 - 179 3.4e-17 jmjN domain
JmjC PF02373 389 - 505 4.7e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 612 - 664 1.7e-16 C5HC2 zinc finger
FYRN PF05964 993 - 1037 5.5e-11 F/Y-rich N-terminus
FYRC PF05965 1042 - 1126 2.1e-20 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AarI CACCTGC 1 cut(s) 2963
AasI GACNNNNNNGTC 1 cut(s) 558
Acc36I ACCTGC 3 cut(s) 380, 2251, 2963
Acc65I GGTACC 1 cut(s) 1405
AccB1I GGYRCC 4 cut(s) 58, 1159, 1405, 3237
AccB7I CCANNNNNTGG 2 cut(s) 407, 3247
AccI GTMKAC 3 cut(s) 493, 621, 2028
AccII CGCG 2 cut(s) 609, 3363
AciI CCGC 4 cut(s) 235, 946, 2944, 3492
AclI AACGTT 1 cut(s) 1512
AclWI GGATC 8 cut(s) 745, 1123, 1721, 2168, 2646, 2957, 3065, 3156
AcsI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2975
AcuI CTGAAG 7 cut(s) 231, 474, 614, 1041, 1679, 2322, 3456
AcvI CACGTG 1 cut(s) 1739
AfiI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3247, 3378
AflII CTTAAG 1 cut(s) 1681
AflIII ACRYGT 1 cut(s) 1738
AhdI GACNNNNNGTC 1 cut(s) 3612
AhlI ACTAGT 1 cut(s) 3512
AjuI GAANNNNNNNTTGG 2 cut(s) 3009, 3041
AleI CACNNNNGTG 1 cut(s) 888
Alw21I GWGCWC 5 cut(s) 1255, 1650, 1949, 2147, 2991
Alw26I GTCTC 5 cut(s) 231, 544, 581, 2280, 3100
AlwI GGATC 8 cut(s) 745, 1123, 1721, 2168, 2646, 2957, 3065, 3156
AlwNI CAGNNNCTG 3 cut(s) 147, 372, 1871
Ama87I CYCGRG 1 cut(s) 1458
AoxI GGCC 8 cut(s) 239, 416, 2058, 3120, 3336, 3489, 3538, 3619
ApeKI GCWGC 5 cut(s) 1616, 1868, 2498, 2528, 3274
ApoI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2975
ArsI GACNNNNNNTTYG 1 cut(s) 3646
AseI ATTAAT 1 cut(s) 3501
Asp700I GAANNNNTTC 2 cut(s) 2235, 3648
Asp718I GGTACC 1 cut(s) 1405
AspLEI GCGC 1 cut(s) 1616
AspS9I GGNCC 5 cut(s) 788, 2204, 2705, 2921, 3125
AsuC2I CCSGG 2 cut(s) 66, 3047
AsuHPI GGTGA 9 cut(s) 115, 947, 1208, 2652, 2944, 3173, 3217, 3312, 3458
AsuII TTCGAA 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 1458
AvaII GGWCC 5 cut(s) 788, 2204, 2705, 2921, 3125
BaeGI GKGCMC 1 cut(s) 1639
BaeI ACNNNNGTAYC 2 cut(s) 1397, 1430
BanI GGYRCC 4 cut(s) 58, 1159, 1405, 3237
BanII GRGCYC 3 cut(s) 1650, 2147, 2480
BauI CACGAG 1 cut(s) 1819
BbrPI CACGTG 1 cut(s) 1739
BbsI GAAGAC 3 cut(s) 42, 2289, 3259
Bbv12I GWGCWC 5 cut(s) 1255, 1650, 1949, 2147, 2991
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 5 cut(s) 1603, 1880, 2485, 2540, 3286
BceAI ACGGC 1 cut(s) 248
BciVI GTATCC 1 cut(s) 1283
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 2 cut(s) 66, 3047
BcoDI GTCTC 5 cut(s) 231, 544, 581, 2280, 3100
BcuI ACTAGT 1 cut(s) 3512
BfaI CTAG 8 cut(s) 1104, 2040, 2045, 2565, 2972, 3513, 3560, 3623
BfmI CTRYAG 4 cut(s) 1551, 2258, 2409, 2499
BfoI RGCGCY 1 cut(s) 1617
BfrI CTTAAG 1 cut(s) 1681
BfuAI ACCTGC 3 cut(s) 380, 2251, 2963
BfuI GTATCC 1 cut(s) 1283
BglI GCCNNNNNGGC 1 cut(s) 3344
BglII AGATCT 1 cut(s) 2047
BisI GCNGC 6 cut(s) 1617, 1869, 2499, 2529, 3275, 3492
BlsI GCNGC 6 cut(s) 1618, 1870, 2500, 2530, 3276, 3493
BmcAI AGTACT 4 cut(s) 163, 2125, 3105, 3377
Bme18I GGWCC 5 cut(s) 788, 2204, 2705, 2921, 3125
BmeRI GACNNNNNGTC 1 cut(s) 3612
BmeT110I CYCGRG 1 cut(s) 1458
BmgT120I GGNCC 5 cut(s) 788, 2204, 2705, 2921, 3125
BmiI GGNNCC 9 cut(s) 60, 808, 912, 1161, 1407, 2922, 2923, 3044, 3239
BmrI ACTGGG 2 cut(s) 2229, 3388
BmuI ACTGGG 2 cut(s) 2229, 3388
BoxI GACNNNNGTC 1 cut(s) 3387
BpiI GAAGAC 3 cut(s) 42, 2289, 3259
BplI GAGNNNNNCTC 2 cut(s) 2973, 3005
BpmI CTGGAG 1 cut(s) 1455
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 593
BpuEI CTTGAG 7 cut(s) 551, 907, 1715, 2083, 2262, 3217, 3390
BpuMI CCSGG 2 cut(s) 66, 3047
BsaAI YACGTR 1 cut(s) 1739
BsaBI GATNNNNATC 2 cut(s) 1857, 3357
BsaI GGTCTC 2 cut(s) 231, 544
BsaJI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2180, 2733, 3281
BsaXI ACNNNNNCTCC 2 cut(s) 1428, 1458
Bsc4I CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3247, 3378
Bse118I RCCGGY 1 cut(s) 3494
Bse1I ACTGG 9 cut(s) 159, 199, 1409, 2010, 2224, 2269, 2431, 3323, 3383
Bse3DI GCAATG 2 cut(s) 2871, 3354
Bse8I GATNNNNATC 2 cut(s) 1857, 3357
BseDI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2180, 2733, 3281
BseJI GATNNNNATC 2 cut(s) 1857, 3357
BseLI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3247, 3378
BseMI GCAATG 2 cut(s) 2871, 3354
BseMII CTCAG 3 cut(s) 267, 2784, 3111
BseNI ACTGG 9 cut(s) 159, 199, 1409, 2010, 2224, 2269, 2431, 3323, 3383
BseRI GAGGAG 3 cut(s) 473, 1076, 1774
BseSI GKGCMC 1 cut(s) 1639
BseXI GCAGC 5 cut(s) 1603, 1880, 2485, 2540, 3286
BseYI CCCAGC 3 cut(s) 906, 2690, 3195
BsgI GTGCAG 4 cut(s) 385, 1518, 2043, 3431
Bsh1236I CGCG 2 cut(s) 609, 3363
Bsh1285I CGRYCG 1 cut(s) 3616
BshFI GGCC 8 cut(s) 241, 418, 2060, 3122, 3338, 3491, 3540, 3621
BshNI GGYRCC 4 cut(s) 58, 1159, 1405, 3237
BsiEI CGRYCG 1 cut(s) 3616
BsiHKAI GWGCWC 5 cut(s) 1255, 1650, 1949, 2147, 2991
BsiHKCI CYCGRG 1 cut(s) 1458
BsiSI CCGG 4 cut(s) 65, 3046, 3123, 3495
BslFI GGGAC 5 cut(s) 19, 1587, 2907, 3375, 3395
BslI CCNNNNNNNGG 7 cut(s) 407, 546, 728, 1200, 1570, 3247, 3378
BsmAI GTCTC 5 cut(s) 231, 544, 581, 2280, 3100
BsmFI GGGAC 5 cut(s) 19, 1587, 2907, 3375, 3395
BsmI GAATGC 2 cut(s) 1838, 3484
BsnI GGCC 8 cut(s) 241, 418, 2060, 3122, 3338, 3491, 3540, 3621
Bso31I GGTCTC 2 cut(s) 231, 544
BsoBI CYCGRG 1 cut(s) 1458
Bsp119I TTCGAA 1 cut(s) 593
Bsp1286I GDGCHC 7 cut(s) 1255, 1639, 1650, 1949, 2147, 2480, 2991
Bsp1407I TGTACA 3 cut(s) 1413, 3331, 3579
Bsp19I CCATGG 2 cut(s) 242, 1163
BspACI CCGC 4 cut(s) 235, 946, 2944, 3492
BspANI GGCC 8 cut(s) 241, 418, 2060, 3122, 3338, 3491, 3540, 3621
BspCNI CTCAG 3 cut(s) 268, 2785, 3110
BspFNI CGCG 2 cut(s) 609, 3363
BspHI TCATGA 2 cut(s) 15, 3484
BspLI GGNNCC 9 cut(s) 60, 808, 912, 1161, 1407, 2922, 2923, 3044, 3239
BspMAI CTGCAG 2 cut(s) 2262, 2503
BspMI ACCTGC 3 cut(s) 380, 2251, 2963
BspPI GGATC 8 cut(s) 745, 1123, 1721, 2168, 2646, 2957, 3065, 3156
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 4 cut(s) 58, 1159, 1405, 3237
BspTI CTTAAG 1 cut(s) 1681
BspTNI GGTCTC 2 cut(s) 231, 544
BsrDI GCAATG 2 cut(s) 2871, 3354
BsrFI RCCGGY 1 cut(s) 3494
BsrGI TGTACA 3 cut(s) 1413, 3331, 3579
BsrI ACTGG 9 cut(s) 159, 199, 1409, 2010, 2224, 2269, 2431, 3323, 3383
BssAI RCCGGY 1 cut(s) 3494
BssECI CCNNGG 7 cut(s) 242, 1163, 1276, 1785, 2180, 2733, 3281
BssNAI GTATAC 2 cut(s) 494, 2029
BssSI CACGAG 1 cut(s) 1819
BssT1I CCWWGG 3 cut(s) 242, 1163, 2733
Bst1107I GTATAC 2 cut(s) 494, 2029
Bst2BI CACGAG 1 cut(s) 1819
Bst4CI ACNGT 9 cut(s) 177, 205, 261, 1040, 1484, 2783, 3079, 3579, 3610
Bst6I CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3639
BstAFI CTTAAG 1 cut(s) 1681
BstAPI GCANNNNNTGC 2 cut(s) 372, 1842
BstAUI TGTACA 3 cut(s) 1413, 3331, 3579
BstBAI YACGTR 1 cut(s) 1739
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 4 cut(s) 1202, 1873, 1906, 3496
BstDEI CTNAG 8 cut(s) 276, 320, 1717, 1950, 2240, 2360, 2793, 3097
BstDSI CCRYGG 2 cut(s) 242, 1163
BstFNI CGCG 2 cut(s) 609, 3363
BstH2I RGCGCY 1 cut(s) 1617
BstHHI GCGC 1 cut(s) 1616
BstMAI GTCTC 5 cut(s) 231, 544, 581, 2280, 3100
BstMCI CGRYCG 1 cut(s) 3616
BstNSI RCATGY 3 cut(s) 1208, 1243, 1908
BstPAI GACNNNNGTC 1 cut(s) 3387
BstSFI CTRYAG 4 cut(s) 1551, 2258, 2409, 2499
BstSLI GKGCMC 1 cut(s) 1639
BstUI CGCG 2 cut(s) 609, 3363
BstV1I GCAGC 5 cut(s) 1603, 1880, 2485, 2540, 3286
BstV2I GAAGAC 3 cut(s) 42, 2289, 3259
BstX2I RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2173
BstXI CCANNNNNNTGG 1 cut(s) 2452
BstYI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2173
BstZ17I GTATAC 2 cut(s) 494, 2029
BsuI GTATCC 1 cut(s) 1283
BsuRI GGCC 8 cut(s) 241, 418, 2060, 3122, 3338, 3491, 3540, 3621
BtgI CCRYGG 2 cut(s) 242, 1163
BtsI GCAGTG 2 cut(s) 1144, 2244
BtsIMutI CAGTG 6 cut(s) 1144, 1416, 2003, 2244, 3158, 3575
BveI ACCTGC 3 cut(s) 380, 2251, 2963
Cac8I GCNNGC 4 cut(s) 1202, 1873, 1906, 3496
CaiI CAGNNNCTG 3 cut(s) 147, 372, 1871
CciI TCATGA 2 cut(s) 15, 3484
CfoI GCGC 1 cut(s) 1616
Cfr10I RCCGGY 1 cut(s) 3494
Cfr13I GGNCC 5 cut(s) 788, 2204, 2705, 2921, 3125
CseI GACGC 1 cut(s) 598
CsiI ACCWGGT 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 2826, 2861
DdeI CTNAG 8 cut(s) 276, 320, 1717, 1950, 2240, 2360, 2793, 3097
DraI TTTAAA 2 cut(s) 1233, 2170
DrdI GACNNNNNNGTC 1 cut(s) 558
DriI GACNNNNNGTC 1 cut(s) 3612
DseDI GACNNNNNNGTC 1 cut(s) 558
Eam1104I CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3639
Eam1105I GACNNNNNGTC 1 cut(s) 3612
EarI CTCTTC 5 cut(s) 450, 547, 1296, 1967, 3639
EciI GGCGGA 2 cut(s) 250, 961
Ecl136II GAGCTC 2 cut(s) 1648, 2145
Eco130I CCWWGG 3 cut(s) 242, 1163, 2733
Eco147I AGGCCT 3 cut(s) 418, 3540, 3621
Eco24I GRGCYC 3 cut(s) 1650, 2147, 2480
Eco31I GGTCTC 2 cut(s) 231, 544
Eco47I GGWCC 5 cut(s) 788, 2204, 2705, 2921, 3125
Eco53kI GAGCTC 2 cut(s) 1648, 2145
Eco57I CTGAAG 7 cut(s) 231, 474, 614, 1041, 1679, 2322, 3456
Eco72I CACGTG 1 cut(s) 1739
Eco88I CYCGRG 1 cut(s) 1458
EcoICRI GAGCTC 2 cut(s) 1648, 2145
EcoO109I RGGNCCY 1 cut(s) 2921
EcoT14I CCWWGG 3 cut(s) 242, 1163, 2733
EcoT22I ATGCAT 2 cut(s) 1245, 1906
EcoT38I GRGCYC 3 cut(s) 1650, 2147, 2480
ErhI CCWWGG 3 cut(s) 242, 1163, 2733
FaqI GGGAC 5 cut(s) 19, 1587, 2907, 3375, 3395
FauNDI CATATG 2 cut(s) 514, 2293
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 3 cut(s) 493, 621, 2028
Fnu4HI GCNGC 6 cut(s) 1617, 1869, 2499, 2529, 3275, 3492
FriOI GRGCYC 3 cut(s) 1650, 2147, 2480
Fsp4HI GCNGC 6 cut(s) 1617, 1869, 2499, 2529, 3275, 3492
FspBI CTAG 8 cut(s) 1104, 2040, 2045, 2565, 2972, 3513, 3560, 3623
GlaI GCGC 1 cut(s) 1615
GluI GCNGC 6 cut(s) 1617, 1869, 2499, 2529, 3275, 3492
GsaI CCCAGC 3 cut(s) 910, 2694, 3199
GsuI CTGGAG 1 cut(s) 1455
HaeII RGCGCY 1 cut(s) 1617
HaeIII GGCC 8 cut(s) 241, 418, 2060, 3122, 3338, 3491, 3540, 3621
HapII CCGG 4 cut(s) 65, 3046, 3123, 3495
HgaI GACGC 1 cut(s) 598
HhaI GCGC 1 cut(s) 1616
Hin6I GCGC 1 cut(s) 1614
HinP1I GCGC 1 cut(s) 1614
HincII GTYRAC 4 cut(s) 622, 1510, 2217, 2344
HindII GTYRAC 4 cut(s) 622, 1510, 2217, 2344
HpaI GTTAAC 1 cut(s) 1510
HpaII CCGG 4 cut(s) 65, 3046, 3123, 3495
HphI GGTGA 9 cut(s) 115, 947, 1208, 2652, 2944, 3173, 3217, 3312, 3458
Hpy166II GTNNAC 9 cut(s) 494, 622, 798, 1414, 1510, 2029, 2217, 2344, 2729
Hpy8I GTNNAC 9 cut(s) 494, 622, 798, 1414, 1510, 2029, 2217, 2344, 2729
Hpy99I CGWCG 2 cut(s) 3472, 3663
HpyAV CCTTC 6 cut(s) 2003, 2003, 2911, 3272, 3468, 3544
HpyCH4III ACNGT 9 cut(s) 177, 205, 261, 1040, 1484, 2783, 3079, 3579, 3610
HpyCH4IV ACGT 3 cut(s) 1512, 1738, 3668
HpyF3I CTNAG 8 cut(s) 276, 320, 1717, 1950, 2240, 2360, 2793, 3097
HpySE526I ACGT 3 cut(s) 1512, 1738, 3668
HspAI GCGC 1 cut(s) 1614
KflI GGGWCCC 1 cut(s) 2921
KpnI GGTACC 1 cut(s) 1409
KroI GCCGGC 1 cut(s) 3494
KroNI GCCGGC 1 cut(s) 3496
Ksp22I TGATCA 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 1510
Lsp1109I GCAGC 5 cut(s) 1603, 1880, 2485, 2540, 3286
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 8 cut(s) 1104, 2040, 2045, 2565, 2972, 3513, 3560, 3623
MaeII ACGT 3 cut(s) 1512, 1738, 3668
MaeIII GTNAC 8 cut(s) 881, 1366, 2518, 2753, 2777, 3008, 3593, 3625
MfeI CAATTG 2 cut(s) 1492, 2161
MflI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2173
MhlI GDGCHC 7 cut(s) 1255, 1639, 1650, 1949, 2147, 2480, 2991
MlyI GAGTC 6 cut(s) 83, 627, 2991, 3225, 3295, 3377
Mph1103I ATGCAT 2 cut(s) 1245, 1906
MroNI GCCGGC 1 cut(s) 3494
MroXI GAANNNNTTC 2 cut(s) 2235, 3648
MslI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2724, 2773
MspA1I CMGCKG 2 cut(s) 1871, 2531
MspCI CTTAAG 1 cut(s) 1681
MspI CCGG 4 cut(s) 65, 3046, 3123, 3495
MunI CAATTG 2 cut(s) 1492, 2161
Mva1269I GAATGC 2 cut(s) 1838, 3484
MvnI CGCG 2 cut(s) 609, 3363
NaeI GCCGGC 1 cut(s) 3496
NciI CCSGG 2 cut(s) 66, 3047
NcoI CCATGG 2 cut(s) 242, 1163
NdeI CATATG 2 cut(s) 514, 2293
NgoMIV GCCGGC 1 cut(s) 3494
NlaIV GGNNCC 9 cut(s) 60, 808, 912, 1161, 1407, 2922, 2923, 3044, 3239
NmeAIII GCCGAG 1 cut(s) 2285
NmuCI GTSAC 6 cut(s) 881, 2518, 2753, 2777, 3593, 3625
NsiI ATGCAT 2 cut(s) 1245, 1906
NspI RCATGY 3 cut(s) 1208, 1243, 1908
NspV TTCGAA 1 cut(s) 593
OliI CACNNNNGTG 1 cut(s) 888
PaeI GCATGC 1 cut(s) 1908
PaeR7I CTCGAG 1 cut(s) 1458
PagI TCATGA 2 cut(s) 15, 3484
PaqCI CACCTGC 1 cut(s) 2963
PceI AGGCCT 3 cut(s) 418, 3540, 3621
PctI GAATGC 2 cut(s) 1838, 3484
PdiI GCCGGC 1 cut(s) 3496
PdmI GAANNNNTTC 2 cut(s) 2235, 3648
PflMI CCANNNNNTGG 2 cut(s) 407, 3247
PfoI TCCNGGA 3 cut(s) 426, 1432, 2747
PkrI GCNGC 6 cut(s) 1618, 1870, 2500, 2530, 3276, 3493
PleI GAGTC 6 cut(s) 82, 626, 2990, 3224, 3294, 3377
PmaCI CACGTG 1 cut(s) 1739
PmlI CACGTG 1 cut(s) 1739
PpsI GAGTC 6 cut(s) 82, 626, 2990, 3224, 3294, 3377
Ppu21I YACGTR 1 cut(s) 1739
PpuMI RGGWCCY 1 cut(s) 2921
PshAI GACNNNNGTC 1 cut(s) 3387
PshBI ATTAAT 1 cut(s) 3501
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 2 cut(s) 1650, 2147
Psp1406I AACGTT 1 cut(s) 1512
Psp5II RGGWCCY 1 cut(s) 2921
PspCI CACGTG 1 cut(s) 1739
PspFI CCCAGC 3 cut(s) 906, 2690, 3195
PspN4I GGNNCC 9 cut(s) 60, 808, 912, 1161, 1407, 2922, 2923, 3044, 3239
PspPI GGNCC 5 cut(s) 788, 2204, 2705, 2921, 3125
PspPPI RGGWCCY 1 cut(s) 2921
PspXI VCTCGAGB 1 cut(s) 1458
PstI CTGCAG 2 cut(s) 2262, 2503
PstNI CAGNNNCTG 3 cut(s) 147, 372, 1871
PsuI RGATCY 5 cut(s) 737, 1115, 1713, 2047, 2173
PvuII CAGCTG 2 cut(s) 1871, 2531
RseI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2724, 2773
SacI GAGCTC 2 cut(s) 1650, 2147
SalI GTCGAC 1 cut(s) 620
SatI GCNGC 6 cut(s) 1617, 1869, 2499, 2529, 3275, 3492
Sau96I GGNCC 5 cut(s) 788, 2204, 2705, 2921, 3125
ScaI AGTACT 4 cut(s) 163, 2125, 3105, 3377
SchI GAGTC 6 cut(s) 83, 627, 2991, 3225, 3295, 3377
SduI GDGCHC 7 cut(s) 1255, 1639, 1650, 1949, 2147, 2480, 2991
SexAI ACCWGGT 1 cut(s) 784
SfcI CTRYAG 4 cut(s) 1551, 2258, 2409, 2499
Sfr274I CTCGAG 1 cut(s) 1458
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 5 cut(s) 788, 2204, 2705, 2921, 3125
SlaI CTCGAG 1 cut(s) 1458
SmiMI CAYNNNNRTG 5 cut(s) 704, 888, 1248, 2724, 2773
SmlI CTYRAG 9 cut(s) 566, 886, 1458, 1681, 1730, 2062, 2277, 3232, 3405
SmoI CTYRAG 9 cut(s) 566, 886, 1458, 1681, 1730, 2062, 2277, 3232, 3405
SpeI ACTAGT 1 cut(s) 3512
SphI GCATGC 1 cut(s) 1908
SseBI AGGCCT 3 cut(s) 418, 3540, 3621
SsiI CCGC 4 cut(s) 235, 946, 2944, 3492
SspI AATATT 4 cut(s) 441, 928, 941, 2350
SspMI CTAG 8 cut(s) 1104, 2040, 2045, 2565, 2972, 3513, 3560, 3623
SstI GAGCTC 2 cut(s) 1650, 2147
StuI AGGCCT 3 cut(s) 418, 3540, 3621
StyI CCWWGG 3 cut(s) 242, 1163, 2733
TaaI ACNGT 9 cut(s) 177, 205, 261, 1040, 1484, 2783, 3079, 3579, 3610
TaiI ACGT 3 cut(s) 1515, 1741, 3671
TaqI TCGA 8 cut(s) 593, 621, 637, 1459, 1998, 3069, 3522, 3616
TaqII GACCGA 1 cut(s) 3602
TatI WGTACW 9 cut(s) 161, 1224, 1413, 2123, 3054, 3103, 3331, 3375, 3579
TauI GCSGC 1 cut(s) 3494
TscAI CASTG 6 cut(s) 1144, 1416, 2010, 2251, 3158, 3582
TseFI GTSAC 6 cut(s) 881, 2518, 2753, 2777, 3593, 3625
TseI GCWGC 5 cut(s) 1616, 1868, 2498, 2528, 3274
Tsp45I GTSAC 6 cut(s) 881, 2518, 2753, 2777, 3593, 3625
TspGWI ACGGA 3 cut(s) 980, 1955, 2547
TspRI CASTG 6 cut(s) 1144, 1416, 2010, 2251, 3158, 3582
Van91I CCANNNNNTGG 2 cut(s) 407, 3247
Vha464I CTTAAG 1 cut(s) 1681
VpaK11BI GGWCC 5 cut(s) 788, 2204, 2705, 2921, 3125
VspI ATTAAT 1 cut(s) 3501
XapI RAATTY 6 cut(s) 47, 310, 753, 1295, 1766, 2975
XbaI TCTAGA 1 cut(s) 2971
XceI RCATGY 3 cut(s) 1208, 1243, 1908
XcmI CCANNNNNNNNNTGG 3 cut(s) 2452, 3182, 3346
XhoI CTCGAG 1 cut(s) 1458
XmiI GTMKAC 3 cut(s) 493, 621, 2028
XmnI GAANNNNTTC 2 cut(s) 2235, 3648
XspI CTAG 8 cut(s) 1104, 2040, 2045, 2565, 2972, 3513, 3560, 3623
ZrmI AGTACT 4 cut(s) 163, 2125, 3105, 3377
Zsp2I ATGCAT 2 cut(s) 1245, 1906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.