RchiOBHm_Chr3g0484911

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005324
Physical Location & Seq
Reverse (-)
31404698 .. 31405814
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGAAACAGAACAAGGAAACCATGGCTCCCCAGGACATATCCTCAGGGGAGAATCAACTCCTATTTCTGAAGGTGCCATGCGAGGATGATGATTCTTTGCAGAAGTGGCAGAAGCAACCTGCAGAGTCTGTAAAAGAGATACTTGATGCAGAGGACAACAAGGCTTCTCAGCAACTTGTGGATAAGCATGTAGTGATATCTGAGGTAGTGGATGTAAGTGATGACGACGAAAGTGAAGAACCAGAATCTGAAGAAAAGTACGAGATTCCTATTCCCCAGCTGGGAACCTTAATGTGGCATTATTCCGATCCCCAAGGAAACATACAGGGTCCTTTCTCAATCATTTCCTTAAAGCGCTGGAGTGATTCTGACTACTTTCCTCCAGATTTCAAAATTTGGAAGACAGGTCAGAGTAGTAATGAAGCTGTATTATTGACCAAAATTCTTCGTGGGAATTTTCTAAATAAAAGTGTATACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

160

Amino Acids

18.3

Weight (kDa)

4.44

Isoelectric Point (pI)

64.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 99 - 139 3.9e-13 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 474
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 3 cut(s) 393, 441, 454
AcuI CTGAAG 2 cut(s) 89, 270
AfaI GTAC 1 cut(s) 260
AfeI AGCGCT 1 cut(s) 356
AfiI CCNNNNNNNGG 2 cut(s) 281, 294
AgsI TTSAA 1 cut(s) 391
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 2 cut(s) 280, 425
AluI AGCT 2 cut(s) 280, 425
AlwI GGATC 1 cut(s) 302
AlwNI CAGNNNCTG 2 cut(s) 128, 248
Aor51HI AGCGCT 1 cut(s) 356
ApoI RAATTY 3 cut(s) 393, 441, 454
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 329
AvaII GGWCC 1 cut(s) 329
AxyI CCTNAGG 1 cut(s) 43
BanI GGYRCC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 407
BciT130I CCWGG 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 4 cut(s) 27, 75, 286, 330
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 407
BpmI CTGGAG 2 cut(s) 366, 379
BsaJI CCNNGG 3 cut(s) 21, 30, 313
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 294
Bse21I CCTNAGG 1 cut(s) 43
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 21, 30, 313
BseGI GGATG 2 cut(s) 91, 217
BseLI CCNNNNNNNGG 2 cut(s) 281, 294
BseMII CTCAG 3 cut(s) 57, 182, 192
BseYI CCCAGC 2 cut(s) 276, 280
BshNI GGYRCC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 281, 294
Bsp143I GATC 1 cut(s) 307
Bsp19I CCATGG 1 cut(s) 21
BspCNI CTCAG 3 cut(s) 56, 181, 193
BspLI GGNNCC 4 cut(s) 27, 75, 286, 330
BspMAI CTGCAG 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 21, 30, 313
BssMI GATC 1 cut(s) 307
BssNAI GTATAC 1 cut(s) 475
BssT1I CCWWGG 2 cut(s) 21, 313
Bst1107I GTATAC 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 43, 168, 201
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 2 cut(s) 91, 217
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 407
BstZ17I GTATAC 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 2 cut(s) 91, 217
BveI ACCTGC 1 cut(s) 127
CaiI CAGNNNCTG 2 cut(s) 128, 248
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 259
CviAII CATG 3 cut(s) 22, 78, 188
CviJI RGCY 4 cut(s) 26, 164, 280, 425
CviKI_1 RGCY 4 cut(s) 26, 164, 280, 425
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 3 cut(s) 43, 168, 201
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 21, 313
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 329
Eco47III AGCGCT 1 cut(s) 356
Eco57I CTGAAG 2 cut(s) 89, 270
Eco81I CCTNAGG 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 30
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 21, 313
ErhI CCWWGG 2 cut(s) 21, 313
FaeI CATG 3 cut(s) 25, 81, 191
FaiI YATR 6 cut(s) 23, 38, 79, 189, 323, 475
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FatI CATG 3 cut(s) 21, 77, 187
FblI GTMKAC 1 cut(s) 474
FokI GGATG 2 cut(s) 98, 224
GlaI GCGC 1 cut(s) 356
GsaI CCCAGC 2 cut(s) 280, 284
GsuI CTGGAG 2 cut(s) 366, 379
HaeII RGCGCY 1 cut(s) 358
HhaI GCGC 1 cut(s) 357
Hin1II CATG 3 cut(s) 25, 81, 191
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HinfI GANTC 6 cut(s) 52, 92, 125, 245, 265, 365
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 6 cut(s) 69, 202, 250, 307, 370, 411
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 64
HpyCH4V TGCA 3 cut(s) 100, 122, 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 3 cut(s) 43, 168, 201
Hsp92II CATG 3 cut(s) 25, 81, 191
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 31
LweI GCATC 1 cut(s) 136
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 4 cut(s) 248, 263, 412, 437
MluCI AATT 3 cut(s) 393, 441, 454
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 5 cut(s) 52, 76, 145, 196, 390
MseI TTAA 2 cut(s) 290, 350
MspA1I CMGCKG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 106
NcoI CCATGG 1 cut(s) 21
NdeII GATC 1 cut(s) 307
NlaIII CATG 3 cut(s) 25, 81, 191
NlaIV GGNNCC 4 cut(s) 27, 75, 286, 330
NspI RCATGY 1 cut(s) 191
PfeI GAWTC 5 cut(s) 52, 92, 245, 265, 365
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 30
PspFI CCCAGC 2 cut(s) 276, 280
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 4 cut(s) 27, 75, 286, 330
PspPI GGNCC 1 cut(s) 329
PspPPI RGGWCCY 1 cut(s) 329
PstI CTGCAG 1 cut(s) 124
PstNI CAGNNNCTG 2 cut(s) 128, 248
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 2 cut(s) 290, 350
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 32
SetI ASST 7 cut(s) 75, 121, 207, 282, 290, 409, 427
SfaNI GCATC 1 cut(s) 136
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 1 cut(s) 329
Sse9I AATT 3 cut(s) 393, 441, 454
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 21, 313
TasI AATT 3 cut(s) 393, 441, 454
TfiI GAWTC 5 cut(s) 52, 92, 245, 265, 365
Tru1I TTAA 2 cut(s) 290, 350
Tru9I TTAA 2 cut(s) 290, 350
TspDTI ATGAA 2 cut(s) 17, 435
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 3 cut(s) 393, 441, 454
XceI RCATGY 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.