RchiOBHm_Chr2g0085241

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006173
Physical Location & Seq
Reverse (-)
751657 .. 754279
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1383 bp
ATGGCGGAGTCAGTTGGGATGCCTGAGACTGATATAACGGCGTTAACCGAAGCGCTCAAGTCTCAGCAACAGCTGCTGCAGATGCTTTATGCTGACCTTGATCAGGAAAGAGAAGCTTCATCTACTGCAGCTGATGAAGCTCTATCCATGATACTGCGTTTGCAAGGTGAGAAGTCTGCTGTGAAGATGGAGGCCAACCAATACAAGAGATTGGCAGAGGAAAAGATATGTCATGCTGAGGAAGCTCTAGCGATTTTCGAGGATCTTATTTATCAGAAGGAAATGGAAATTGCTTCTTTGGAGTTTCAGCTTCAGGCTTATAGGTACAAACTGTTGAGCTTGGGTGTCAGTACAGAATTAGGTACCACCGAAAATGTGTATCCGGAAAACTTGTTGCAGCAAAGAGGTGATGGGGAAGCGAGTGGTGTTAGTGGGGCAATTAGAAGAATCAACTCCCTGCCTCCACTTGAGCTCAAGGATTTCCTCAACAAGAAATGCCCTGTAGAAAGAGAGAGTGAAAGTCTACCGAAAGAAAAACCTATGATACCAAAGCCAGAATCTTGTATGAAAAAGGGAGACAAAAAGGTGGACAAGGAAGTTACTTTCCAGGGCATTGATGCAGAGAAGAAATCGAGAAATGCTTCCAATGGCAACCTTAATTCGATCTGGGAGCAGATTAAGAAGTTAGACGACCGAGTGAGAGAGATATCAGATTGTAAAGATTCCGGTGGTATAGGAAAATCTGGAATTTTGATGGGTCCGGGTAGGACATGTTCATTACCTCCAATTCCGAAAGTAATCACAAGCACATCCCATGATACAGAAAGTGAAGATATTATTGCTACCTTTGAAAGAGTTGATCATAATGAGAATGTACAGGAAACGGAGGCAATTGTTAGTACTACATGCTCAACTGGCGTCCATGATGTTTTTGAAGTTCCACAGAGCTATGAATGCAGTCCTAAAACTTTGGACAGTCCTAAATCTTTTGAAGGTGAGAAGAAAGAGTTTGATAAATTGACATTTGGTGTTGAGAGTTCTAAAGCTTTTGAAGTTGAGAAGAAAGAATTTATTGGCAAAGATGATTTGATATGTGATGAGATTAGTGAAGTGTATGTTCAAGATGAGACAGAGAGGGTTAAGAAAAGGTTTCATGGCAAGTTGCTCGAGAATAGAATATTGAGTCGAACAACCACTGTTGACTCAACTGCAAGGGAGAGAGTGGCTGCTGCGGTTACTGAGAATCAAGCCAGATTTCAACAGTTGTGGAGGAGGATAGAGCGGCTTGAGGGAGAGAGAAGTAGCGTAAGGAACGAAATAAGTCATGCAGGAGAAGAGGAGTTGAAGTTTTGTTTAGGGATATACGTGAGCAACTTAACTTAA

Protein Analysis

460

Amino Acids

51.71

Weight (kDa)

5.0

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 14 - 104 7.9e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 523
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 3 cut(s) 5, 1232, 1282
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 747, 1067
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 5 cut(s) 326, 352, 364, 876, 901
AfeI AGCGCT 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 770
AgsI TTSAA 7 cut(s) 851, 935, 992, 1052, 1121, 1259, 1345
AjnI CCWGG 1 cut(s) 606
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 20, 66, 570, 1121
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 1166
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 6 cut(s) 73, 76, 128, 397, 1226, 1229
ApoI RAATTY 2 cut(s) 747, 1067
Asp700I GAANNNNTTC 1 cut(s) 640
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 758
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 3 cut(s) 179, 419, 1007
AvaI CYCGRG 1 cut(s) 1166
AvaII GGWCC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
BccI CCATC 3 cut(s) 181, 404, 748
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 2 cut(s) 1147, 1181
BciT130I CCWGG 1 cut(s) 608
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 2 cut(s) 100, 859
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 4 cut(s) 20, 66, 570, 1121
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 3 cut(s) 77, 126, 501
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
BlsI GCNGC 7 cut(s) 75, 78, 130, 399, 1228, 1231, 1284
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 2 cut(s) 608, 762
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 2 cut(s) 364, 759
BmrFI CCNGG 2 cut(s) 608, 762
BmsI GCATC 3 cut(s) 9, 72, 607
Bpu10I CCTNAGC 1 cut(s) 237
BpuEI CTTGAG 4 cut(s) 41, 458, 488, 1307
BpuMI CCSGG 1 cut(s) 762
BsaAI YACGTR 1 cut(s) 1366
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 1 cut(s) 607
BsaWI WCCGGW 2 cut(s) 382, 725
BsaXI ACNNNNNCTCC 2 cut(s) 1331, 1361
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 919
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 2 cut(s) 24, 809
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 4 cut(s) 15, 77, 228, 1230
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 2 cut(s) 1285, 1352
BseXI GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 1166
BsiSI CCGG 3 cut(s) 383, 726, 761
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 4 cut(s) 20, 66, 570, 1121
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 1166
Bsp1286I GDGCHC 1 cut(s) 474
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 874
Bsp143I GATC 4 cut(s) 100, 262, 663, 859
BspACI CCGC 3 cut(s) 5, 1232, 1282
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 4 cut(s) 16, 76, 229, 1231
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 364, 759
BspMAI CTGCAG 2 cut(s) 81, 130
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 1282
BsrGI TGTACA 1 cut(s) 874
BsrI ACTGG 1 cut(s) 919
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 4 cut(s) 100, 262, 663, 859
BssNI GRCGYC 1 cut(s) 918
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 4 cut(s) 333, 977, 1198, 1263
Bst6I CTCTTC 1 cut(s) 1329
BstACI GRCGYC 1 cut(s) 918
BstAPI GCANNNNNTGC 1 cut(s) 73
BstAUI TGTACA 1 cut(s) 874
BstBAI YACGTR 1 cut(s) 1366
BstDEI CTNAG 4 cut(s) 24, 63, 237, 1239
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 24, 809
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 4 cut(s) 103, 265, 666, 862
BstMAI GTCTC 4 cut(s) 20, 66, 570, 1121
BstMBI GATC 4 cut(s) 100, 262, 663, 859
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 2 cut(s) 774, 909
BstSCI CCNGG 2 cut(s) 606, 760
BstSFI CTRYAG 3 cut(s) 77, 126, 501
BstV1I GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 194
BtsCI GGATG 2 cut(s) 24, 809
BtsIMutI CAGTG 1 cut(s) 1194
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 758
CseI GACGC 1 cut(s) 907
Csp6I GTAC 5 cut(s) 325, 351, 363, 875, 900
CspCI CAANNNNNGTGG 2 cut(s) 1247, 1282
CviAII CATG 8 cut(s) 148, 233, 771, 815, 906, 923, 1154, 1325
CviQI GTAC 5 cut(s) 325, 351, 363, 875, 900
DdeI CTNAG 4 cut(s) 24, 63, 237, 1239
DpnI GATC 4 cut(s) 102, 264, 665, 861
DpnII GATC 4 cut(s) 100, 262, 663, 859
Eam1104I CTCTTC 1 cut(s) 1329
EarI CTCTTC 1 cut(s) 1329
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 474
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 758
Eco47III AGCGCT 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 1166
EcoICRI GAGCTC 1 cut(s) 472
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 708
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 8 cut(s) 151, 236, 774, 818, 909, 926, 1157, 1328
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FatI CATG 8 cut(s) 147, 232, 770, 814, 905, 922, 1153, 1324
FbaI TGATCA 2 cut(s) 100, 859
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
FokI GGATG 2 cut(s) 31, 796
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 54
GluI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 383, 726, 761
HgaI GACGC 1 cut(s) 907
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 8 cut(s) 151, 236, 774, 818, 909, 926, 1157, 1328
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 2 cut(s) 45, 1201
HindII GTYRAC 2 cut(s) 45, 1201
HindIII AAGCTT 2 cut(s) 114, 1044
HinfI GANTC 7 cut(s) 8, 447, 557, 722, 1183, 1202, 1243
HpaI GTTAAC 1 cut(s) 45
HpaII CCGG 3 cut(s) 383, 726, 761
HphI GGTGA 3 cut(s) 179, 419, 1007
Hpy166II GTNNAC 4 cut(s) 45, 524, 589, 1201
Hpy188I TCNGA 3 cut(s) 276, 712, 792
Hpy188III TCNNGA 6 cut(s) 104, 383, 633, 744, 1121, 1168
Hpy8I GTNNAC 4 cut(s) 45, 524, 589, 1201
HpyAV CCTTC 2 cut(s) 271, 986
HpyCH4III ACNGT 4 cut(s) 333, 977, 1198, 1263
HpyCH4IV ACGT 1 cut(s) 1365
HpyCH4V TGCA 8 cut(s) 79, 128, 163, 397, 620, 957, 1211, 1328
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
HpyF3I CTNAG 4 cut(s) 24, 63, 237, 1239
HpySE526I ACGT 1 cut(s) 1365
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 8 cut(s) 151, 236, 774, 818, 909, 926, 1157, 1328
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 382
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 2 cut(s) 100, 859
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 100, 262, 663, 859
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
LweI GCATC 3 cut(s) 9, 72, 607
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 1365
MaeIII GTNAC 2 cut(s) 598, 1234
MalI GATC 4 cut(s) 102, 264, 665, 861
MbiI CCGCTC 1 cut(s) 1282
MboI GATC 4 cut(s) 100, 262, 663, 859
MboII GAAGA 7 cut(s) 196, 456, 637, 842, 1012, 1072, 1346
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 9 cut(s) 288, 356, 438, 658, 747, 786, 891, 1016, 1067
MlyI GAGTC 3 cut(s) 17, 1192, 1196
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 6 cut(s) 44, 657, 678, 1140, 1376, 1381
MspA1I CMGCKG 2 cut(s) 73, 131
MspI CCGG 3 cut(s) 383, 726, 761
MspR9I CCNGG 2 cut(s) 608, 762
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
NciI CCSGG 1 cut(s) 762
NdeII GATC 4 cut(s) 100, 262, 663, 859
NlaIII CATG 8 cut(s) 151, 236, 774, 818, 909, 926, 1157, 1328
NlaIV GGNNCC 2 cut(s) 364, 759
NspI RCATGY 2 cut(s) 774, 909
PaeR7I CTCGAG 1 cut(s) 1166
PciI ACATGT 1 cut(s) 770
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 4 cut(s) 447, 557, 722, 1243
PkrI GCNGC 7 cut(s) 75, 78, 130, 399, 1228, 1231, 1284
PleI GAGTC 3 cut(s) 16, 1191, 1196
PpsI GAGTC 3 cut(s) 16, 1191, 1196
Ppu21I YACGTR 1 cut(s) 1366
PscI ACATGT 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 364, 759
PspPI GGNCC 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
PstI CTGCAG 2 cut(s) 81, 130
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 2 cut(s) 73, 131
RsaI GTAC 5 cut(s) 326, 352, 364, 876, 901
RsaNI GTAC 5 cut(s) 325, 351, 363, 875, 900
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 6 cut(s) 44, 657, 678, 1140, 1376, 1381
SatI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
Sau3AI GATC 4 cut(s) 100, 262, 663, 859
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 3 cut(s) 17, 1192, 1196
ScrFI CCNGG 2 cut(s) 608, 762
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 3 cut(s) 9, 72, 607
SfcI CTRYAG 3 cut(s) 77, 126, 501
Sfr274I CTCGAG 1 cut(s) 1166
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 1166
SmlI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
SmoI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
Sse9I AATT 9 cut(s) 288, 356, 438, 658, 747, 786, 891, 1016, 1067
SsiI CCGC 3 cut(s) 5, 1232, 1282
SspI AATATT 1 cut(s) 1179
SspMI CTAG 1 cut(s) 248
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 2 cut(s) 606, 760
TaaI ACNGT 4 cut(s) 333, 977, 1198, 1263
TaiI ACGT 1 cut(s) 1368
TaqI TCGA 5 cut(s) 258, 632, 662, 1167, 1186
TaqII GACCGA 1 cut(s) 708
TasI AATT 9 cut(s) 288, 356, 438, 658, 747, 786, 891, 1016, 1067
TatI WGTACW 3 cut(s) 350, 874, 899
TauI GCSGC 1 cut(s) 1285
TfiI GAWTC 4 cut(s) 447, 557, 722, 1243
Tru1I TTAA 6 cut(s) 44, 657, 678, 1140, 1376, 1381
Tru9I TTAA 6 cut(s) 44, 657, 678, 1140, 1376, 1381
TscAI CASTG 1 cut(s) 1201
TseI GCWGC 6 cut(s) 73, 76, 128, 397, 1226, 1229
TspDTI ATGAA 6 cut(s) 108, 150, 581, 765, 966, 1142
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 1 cut(s) 1201
VpaK11BI GGWCC 1 cut(s) 758
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 2 cut(s) 747, 1067
XceI RCATGY 2 cut(s) 774, 909
XhoI CTCGAG 1 cut(s) 1166
XmiI GTMKAC 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 640
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.