RchiOBHm_Chr2g0145211

Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004126
Physical Location & Seq
Forward (+)
62842838 .. 62845168
2331 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 288 bp
ATGTCTACCGCGGAATCTCCGACTGCTGCTAAGAAAGTGGTCATTCATCTCAGAGCTACTGGCGATGCCCCTATACTGAAGCAAGCCAAATTCAAGATACCTGGAACGGACAAGTTTGCTAAAGTGATTGACTTTTTAAGGAGGCAACTTCACCGGGATACGTTGTTTGTGTATGTCAATAGTGCGTTCTCGCCAAACCCAGATGAATTGGTGATGGATCTGTATAATAATTTTGGTTTTGATGGTAAACTGGTGGTCAATTATGCTTGTTCCATGGCGTGGGGCTAA

Protein Analysis

95

Amino Acids

10.61

Weight (kDa)

9.17

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APG12 PF04110 12 - 95 5.3e-34 Ubiquitin-like autophagy protein Apg12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 279
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 9, 11
AclWI GGATC 1 cut(s) 225
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
AlwI GGATC 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 1 cut(s) 89
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 143, 223
BbvI GCAGC 1 cut(s) 13
BccI CCATC 2 cut(s) 208, 236
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 151
BcnI CCSGG 1 cut(s) 155
BfuI GTATCC 1 cut(s) 151
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 2 cut(s) 102, 155
BmrFI CCNGG 2 cut(s) 102, 155
BmsI GCATC 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 9, 273
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 2 cut(s) 64, 255
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 9, 273
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 2 cut(s) 64, 255
BseXI GCAGC 1 cut(s) 13
Bsh1236I CGCG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 279
Bsp143I GATC 1 cut(s) 217
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 9, 11
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 1 cut(s) 11
BspPI GGATC 1 cut(s) 225
BsrI ACTGG 2 cut(s) 64, 255
BssECI CCNNGG 2 cut(s) 9, 273
BssMI GATC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 30, 50
BstDSI CCRYGG 2 cut(s) 9, 273
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 1 cut(s) 220
BstMBI GATC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 100, 153
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 13
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuI GTATCC 1 cut(s) 151
BtgI CCRYGG 2 cut(s) 9, 273
BtgZI GCGATG 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 84
Cfr42I CCGCGG 1 cut(s) 12
CviAII CATG 1 cut(s) 274
CviJI RGCY 3 cut(s) 56, 86, 285
CviKI_1 RGCY 3 cut(s) 56, 86, 285
DdeI CTNAG 2 cut(s) 30, 50
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 1 cut(s) 277
FaiI YATR 5 cut(s) 74, 174, 225, 264, 275
FatI CATG 1 cut(s) 273
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 27
HapII CCGG 1 cut(s) 154
Hin1II CATG 1 cut(s) 277
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 154
HphI GGTGA 2 cut(s) 143, 223
Hpy166II GTNNAC 2 cut(s) 6, 248
Hpy188I TCNGA 2 cut(s) 21, 53
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 2 cut(s) 6, 248
HpyCH4IV ACGT 1 cut(s) 161
HpyF3I CTNAG 2 cut(s) 30, 50
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 1 cut(s) 277
KspI CCGCGG 1 cut(s) 12
Kzo9I GATC 1 cut(s) 217
LpnPI CCDG 6 cut(s) 45, 87, 114, 167, 213, 236
Lsp1109I GCAGC 1 cut(s) 13
LweI GCATC 1 cut(s) 55
MaeII ACGT 1 cut(s) 161
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MflI RGATCY 1 cut(s) 217
MluCI AATT 4 cut(s) 89, 206, 229, 259
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 1 cut(s) 135
MseI TTAA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 2 cut(s) 102, 155
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 11
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 273
NdeII GATC 1 cut(s) 217
NlaIII CATG 1 cut(s) 277
PfeI GAWTC 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 279
PkrI GCNGC 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 217
SacII CCGCGG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 217
ScrFI CCNGG 2 cut(s) 102, 155
SetI ASST 3 cut(s) 58, 103, 164
SfaNI GCATC 1 cut(s) 55
Sfr303I CCGCGG 1 cut(s) 12
SgrBI CCGCGG 1 cut(s) 12
Sse9I AATT 4 cut(s) 89, 206, 229, 259
SsiI CCGC 2 cut(s) 9, 11
StyD4I CCNGG 2 cut(s) 100, 153
StyI CCWWGG 1 cut(s) 273
TaiI ACGT 1 cut(s) 164
TasI AATT 4 cut(s) 89, 206, 229, 259
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 35, 219
TspGWI ACGGA 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 279
XapI RAATTY 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.