RchiOBHm_Chr2g0176701

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005131
Physical Location & Seq
Forward (+)
88406696 .. 88408275
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGCAGCTCTAATGTACCAGATATTGTCGTCCTCAGCGCTGGTATCTCTGGGACTCTATCATATGGTGGCCACCACCCGCAACTACCTGAAATCTCCCCAGTCCTATTCCGCTAAGCCCTATCATCCCTTTCCTCTCTCATCTTCTTCCCCTAATCATCGCCTCAGGTACGTCCAGCTGTTCCTCATCACCGCCTGCCTTATCGTCGCCGTCGTCCACCAGTCGGCGGCCTCCTTGGACGCTGACCCACTCCTCAAGGGCCGCACCCCTGTCCACCGCTTCACCTCCCTCCAGTCCGCCGCTGCTCTCTTCCTCTTTCTGCTCCTCACGCTCGCCATCCTCCTCTCCGAGTGCGCCCCTTCTCTGCTCCCCCTCTCCACCGATCTCATCTTCGGCCTCGCCGCCGCGCTCTTCTACATCCACTCCTTCATCTCTCGGGAAGCTGCCTCCGTCCAGACCTCCGATCTGCAGGCCAAGTGCGATTCCGTCTCGGGCCGGGTCTCCGCACTGGCCTCCCTGCTCTGCCTTGTCTTGGCCTGCCAGCCCAAGCTCTTTGTTGCTGATGTGGGTTTGGGCGCTACCATGTCCCTGCAGGGCCTCTGGGTCTTGCAGACCGGGCTCTCCCTTTACGCGGATGCCTTCATCCCTGAGGGCTGCCACAAGCTACTTGATGTCGTCAGCGGGGTTGAGGGTTCAACCAAGTGCGACCTGGATGAGTCCCGCTTCAGGGCAGTTGCTATTTTGGATCTCTTGTTCCTGGTGCACGTCATGTTTGTGTTGCTCATTATGCTGGTCGTCCATGCAGTGGTTGCCAAGTCTGTTGGCATCCGCAAGTTGGCTTCTTACGAGGCCTTGCCTACCGCTGTACCCGACAACAACCACAATCATATTCAGATGAAAGCCTTGAGCGGCACCCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

307

Amino Acids

33.01

Weight (kDa)

8.15

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 128 - 266 5.1e-31 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 805
AccBSI CCGCTC 1 cut(s) 909
AccII CGCG 2 cut(s) 407, 632
AclWI GGATC 1 cut(s) 753
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 3 cut(s) 17, 170, 867
AfeI AGCGCT 1 cut(s) 39
AfiI CCNNNNNNNGG 8 cut(s) 223, 226, 532, 631, 726, 727, 805, 835
AgsI TTSAA 1 cut(s) 696
AjiI CACGTC 1 cut(s) 766
AjnI CCWGG 3 cut(s) 708, 756, 915
AloI GAACNNNNNNTCC 2 cut(s) 737, 769
AluBI AGCT 5 cut(s) 8, 178, 443, 550, 664
AluI AGCT 5 cut(s) 8, 178, 443, 550, 664
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 2 cut(s) 493, 505
Alw44I GTGCAC 1 cut(s) 761
AlwI GGATC 1 cut(s) 753
Ama87I CYCGRG 2 cut(s) 435, 490
Aor51HI AGCGCT 1 cut(s) 39
ApaLI GTGCAC 1 cut(s) 761
ApeKI GCWGC 4 cut(s) 5, 302, 443, 654
AspLEI GCGC 4 cut(s) 40, 356, 409, 578
AspS9I GGNCC 3 cut(s) 259, 493, 595
AsuC2I CCSGG 2 cut(s) 497, 616
AsuHPI GGTGA 2 cut(s) 181, 274
AvaI CYCGRG 2 cut(s) 435, 490
AxyI CCTNAGG 2 cut(s) 164, 648
BaeGI GKGCMC 1 cut(s) 765
BaeI ACNNNNGTAYC 2 cut(s) 849, 882
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 1 cut(s) 621
Bbv12I GWGCWC 1 cut(s) 765
BbvCI CCTCAGC 1 cut(s) 34
BbvI GCAGC 4 cut(s) 17, 289, 430, 641
BccI CCATC 1 cut(s) 344
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 3 cut(s) 710, 758, 917
BcnI CCSGG 2 cut(s) 497, 616
BcoDI GTCTC 2 cut(s) 493, 505
BfmI CTRYAG 2 cut(s) 467, 590
BfoI RGCGCY 2 cut(s) 41, 579
BlpI GCTNAGC 1 cut(s) 114
Bme1390I CCNGG 5 cut(s) 497, 616, 710, 758, 917
BmeT110I CYCGRG 2 cut(s) 435, 490
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 3 cut(s) 259, 493, 595
BmiI GGNNCC 1 cut(s) 913
BmrFI CCNGG 5 cut(s) 497, 616, 710, 758, 917
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 2 cut(s) 625, 834
BmuI ACTGGG 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 34
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 497, 616
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 2 cut(s) 234, 915
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 8 cut(s) 223, 226, 532, 631, 726, 727, 805, 835
Bse1I ACTGG 4 cut(s) 100, 220, 292, 513
Bse21I CCTNAGG 2 cut(s) 164, 648
BseBI CCWGG 3 cut(s) 710, 758, 917
BseDI CCNNGG 2 cut(s) 234, 915
BseGI GGATG 7 cut(s) 124, 336, 417, 640, 642, 718, 825
BseLI CCNNNNNNNGG 8 cut(s) 223, 226, 532, 631, 726, 727, 805, 835
BseMII CTCAG 3 cut(s) 48, 178, 639
BseNI ACTGG 4 cut(s) 100, 220, 292, 513
BseRI GAGGAG 3 cut(s) 242, 314, 332
BseSI GKGCMC 1 cut(s) 765
BseXI GCAGC 4 cut(s) 17, 289, 430, 641
Bsh1236I CGCG 2 cut(s) 407, 632
BshNI GGYRCC 1 cut(s) 911
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 2 cut(s) 435, 490
BsiSI CCGG 2 cut(s) 496, 615
BslFI GGGAC 3 cut(s) 66, 571, 703
BslI CCNNNNNNNGG 8 cut(s) 223, 226, 532, 631, 726, 727, 805, 835
BsmAI GTCTC 2 cut(s) 493, 505
BsmBI CGTCTC 1 cut(s) 493
BsmFI GGGAC 3 cut(s) 66, 571, 703
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 2 cut(s) 435, 490
Bsp1286I GDGCHC 2 cut(s) 621, 765
Bsp143I GATC 3 cut(s) 382, 463, 745
Bsp1720I GCTNAGC 1 cut(s) 114
BspCNI CTCAG 3 cut(s) 47, 177, 640
BspFNI CGCG 2 cut(s) 407, 632
BspLI GGNNCC 1 cut(s) 913
BspMAI CTGCAG 2 cut(s) 471, 594
BspPI GGATC 1 cut(s) 753
BspQI GCTCTTC 1 cut(s) 416
BspT107I GGYRCC 1 cut(s) 911
BspTNI GGTCTC 1 cut(s) 505
BsrBI CCGCTC 1 cut(s) 909
BsrI ACTGG 4 cut(s) 100, 220, 292, 513
BssECI CCNNGG 2 cut(s) 234, 915
BssMI GATC 3 cut(s) 382, 463, 745
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 3 cut(s) 710, 758, 917
Bst6I CTCTTC 2 cut(s) 314, 416
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 5 cut(s) 196, 333, 471, 538, 542
BstDEI CTNAG 5 cut(s) 34, 114, 164, 648, 921
BstF5I GGATG 7 cut(s) 124, 336, 417, 640, 642, 718, 825
BstFNI CGCG 2 cut(s) 407, 632
BstH2I RGCGCY 2 cut(s) 41, 579
BstHHI GCGC 4 cut(s) 40, 356, 409, 578
BstKTI GATC 3 cut(s) 385, 466, 748
BstMAI GTCTC 2 cut(s) 493, 505
BstMBI GATC 3 cut(s) 382, 463, 745
BstMWI GCNNNNNNNGC 4 cut(s) 328, 616, 787, 809
BstNI CCWGG 3 cut(s) 710, 758, 917
BstSCI CCNGG 5 cut(s) 495, 614, 708, 756, 915
BstSFI CTRYAG 2 cut(s) 467, 590
BstSLI GKGCMC 1 cut(s) 765
BstUI CGCG 2 cut(s) 407, 632
BstV1I GCAGC 4 cut(s) 17, 289, 430, 641
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu36I CCTNAGG 2 cut(s) 164, 648
BtgZI GCGATG 1 cut(s) 143
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 7 cut(s) 124, 336, 417, 640, 642, 718, 825
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 2 cut(s) 506, 810
Cac8I GCNNGC 5 cut(s) 196, 333, 471, 538, 542
CfoI GCGC 4 cut(s) 40, 356, 409, 578
Cfr13I GGNCC 3 cut(s) 259, 493, 595
CseI GACGC 1 cut(s) 248
Csp6I GTAC 3 cut(s) 16, 169, 866
CviAII CATG 3 cut(s) 583, 769, 800
CviQI GTAC 3 cut(s) 16, 169, 866
DdeI CTNAG 5 cut(s) 34, 114, 164, 648, 921
DpnI GATC 3 cut(s) 384, 465, 747
DpnII GATC 3 cut(s) 382, 463, 745
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 314, 416
EarI CTCTTC 2 cut(s) 314, 416
EciI GGCGGA 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 234
Eco147I AGGCCT 1 cut(s) 851
Eco24I GRGCYC 1 cut(s) 621
Eco31I GGTCTC 1 cut(s) 505
Eco47III AGCGCT 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 709
Eco81I CCTNAGG 2 cut(s) 164, 648
Eco88I CYCGRG 2 cut(s) 435, 490
EcoO109I RGGNCCY 1 cut(s) 595
EcoRII CCWGG 3 cut(s) 708, 756, 915
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 493
FaeI CATG 3 cut(s) 586, 772, 803
FaiI YATR 7 cut(s) 63, 65, 584, 770, 788, 801, 888
FaqI GGGAC 3 cut(s) 66, 571, 703
FatI CATG 3 cut(s) 582, 768, 799
FauI CCCGC 3 cut(s) 86, 674, 728
FauNDI CATATG 1 cut(s) 63
FokI GGATG 7 cut(s) 111, 323, 404, 629, 647, 725, 812
FriOI GRGCYC 1 cut(s) 621
GlaI GCGC 4 cut(s) 39, 355, 408, 577
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 41, 579
HapII CCGG 2 cut(s) 496, 615
HgaI GACGC 1 cut(s) 248
HhaI GCGC 4 cut(s) 40, 356, 409, 578
Hin1II CATG 3 cut(s) 586, 772, 803
Hin6I GCGC 4 cut(s) 38, 354, 407, 576
HinP1I GCGC 4 cut(s) 38, 354, 407, 576
HinfI GANTC 3 cut(s) 54, 482, 716
HpaII CCGG 2 cut(s) 496, 615
HphI GGTGA 2 cut(s) 181, 274
Hpy166II GTNNAC 3 cut(s) 217, 274, 763
Hpy188I TCNGA 3 cut(s) 349, 463, 894
Hpy188III TCNNGA 2 cut(s) 437, 454
Hpy8I GTNNAC 3 cut(s) 217, 274, 763
Hpy99I CGWCG 2 cut(s) 209, 215
HpyAV CCTTC 3 cut(s) 369, 436, 649
HpyCH4IV ACGT 2 cut(s) 171, 765
HpyCH4V TGCA 5 cut(s) 469, 592, 610, 763, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 328, 616, 787, 809
HpyF3I CTNAG 5 cut(s) 34, 114, 164, 648, 921
HpySE526I ACGT 2 cut(s) 171, 765
Hsp92II CATG 3 cut(s) 586, 772, 803
HspAI GCGC 4 cut(s) 38, 354, 407, 576
Kzo9I GATC 3 cut(s) 382, 463, 745
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 2 cut(s) 327, 372
Lsp1109I GCAGC 4 cut(s) 17, 289, 430, 641
LweI GCATC 2 cut(s) 625, 834
MaeII ACGT 2 cut(s) 171, 765
MalI GATC 3 cut(s) 384, 465, 747
MbiI CCGCTC 1 cut(s) 909
MboI GATC 3 cut(s) 382, 463, 745
MboII GAAGA 5 cut(s) 135, 138, 301, 382, 403
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 2 cut(s) 621, 765
MlsI TGGCCA 1 cut(s) 71
MluNI TGGCCA 1 cut(s) 71
MlyI GAGTC 2 cut(s) 48, 725
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MslI CAYNNNNRTG 1 cut(s) 773
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 4 cut(s) 178, 302, 681, 863
MspI CCGG 2 cut(s) 496, 615
MspR9I CCNGG 5 cut(s) 497, 616, 710, 758, 917
MvaI CCWGG 3 cut(s) 710, 758, 917
MvnI CGCG 2 cut(s) 407, 632
MwoI GCNNNNNNNGC 4 cut(s) 328, 616, 787, 809
NciI CCSGG 2 cut(s) 497, 616
NdeI CATATG 1 cut(s) 63
NdeII GATC 3 cut(s) 382, 463, 745
NlaIII CATG 3 cut(s) 586, 772, 803
NlaIV GGNNCC 1 cut(s) 913
PceI AGGCCT 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 1 cut(s) 482
PflMI CCANNNNNTGG 1 cut(s) 805
PleI GAGTC 2 cut(s) 48, 724
PpsI GAGTC 2 cut(s) 48, 724
Psp6I CCWGG 3 cut(s) 708, 756, 915
PspGI CCWGG 3 cut(s) 708, 756, 915
PspN4I GGNNCC 1 cut(s) 913
PspPI GGNCC 3 cut(s) 259, 493, 595
PstI CTGCAG 2 cut(s) 471, 594
PsuI RGATCY 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 3 cut(s) 17, 170, 867
RsaNI GTAC 3 cut(s) 16, 169, 866
RseI CAYNNNNRTG 1 cut(s) 773
SapI GCTCTTC 1 cut(s) 416
Sau3AI GATC 3 cut(s) 382, 463, 745
Sau96I GGNCC 3 cut(s) 259, 493, 595
SbfI CCTGCAGG 1 cut(s) 594
SchI GAGTC 2 cut(s) 48, 725
ScrFI CCNGG 5 cut(s) 497, 616, 710, 758, 917
SdaI CCTGCAGG 1 cut(s) 594
SduI GDGCHC 2 cut(s) 621, 765
SfaNI GCATC 2 cut(s) 625, 834
SfcI CTRYAG 2 cut(s) 467, 590
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 2 cut(s) 254, 904
SmoI CTYRAG 2 cut(s) 254, 904
Sse8387I CCTGCAGG 1 cut(s) 594
SseBI AGGCCT 1 cut(s) 851
StuI AGGCCT 1 cut(s) 851
StyD4I CCNGG 5 cut(s) 495, 614, 708, 756, 915
StyI CCWWGG 1 cut(s) 234
TaiI ACGT 2 cut(s) 174, 768
TauI GCSGC 6 cut(s) 230, 264, 302, 404, 407, 912
TfiI GAWTC 1 cut(s) 482
TscAI CASTG 2 cut(s) 513, 810
TseI GCWGC 4 cut(s) 5, 302, 443, 654
TspDTI ATGAA 3 cut(s) 418, 631, 911
TspGWI ACGGA 2 cut(s) 439, 475
TspRI CASTG 2 cut(s) 513, 810
Van91I CCANNNNNTGG 1 cut(s) 805
VneI GTGCAC 1 cut(s) 761
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.