RchiOBHm_Chr1g0336591

Phosphoglucan phosphatase DSP4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009617
Physical Location & Seq
Forward (+)
28303481 .. 28310991
7511 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGAACTGTCTCCAGAATCTTCCAGGGTCCTCTGTTTTGCCGCTGCGGGGTTTCGTATGCAATCCCAGGAAGCCGTCCTTCTCCTCCTCCTCCTCCTCCGCCACTACTAGTCTGGGAATGATGACTAGTAATACCGATCCACCAAGAAGCATGGCTGTCAAGGCAGTTTCTGGTTCCACTCCCAGTGCTGAGACAAGTGGTGCTGAAGTGAAGGAGGAAAAATCTGAGGTATATAGTAATGTCATGACAGAGGCCATGGGTGCTGTCCTGACCTATAGGCATGAGCTGGGTATGAACTACAACTTCATACGTCCAGATCTGATTGTGGGGTCTTGCCTTCAGACTCCAAAAGATGTTGACAAGCTTCGCAGCATTGGAGTGAAGACTATTTTCTGCTTGCAACAAAATTCAGATTTGGAATATTTTGGGGTCGACATTGGTGCCATTATAGATTATGCCAAAGAATTTGATGACATTGAACACTTGCGTGCTGAAATAAGGGACTTTGATTCATATGATCTACGTCTCCGGCTTCCTGCTGTTGTTAGTAAATTATATAAGGCCATAAACAGAAATGGAGGTGTCACTTATGTACATTGCACTGCTGGACTTGGAAGAGCTCCTGCTACTGCGTTGGCTTACATGTTCTGGGTACAAGGATACAAACTTGAAGATGCGGTCAAATTACTTCTGAGCAAACGTTCATGCTTCCCAAAACTGGAGGCTATAAAAACCGCAACTGCTGATATTCTCACAGGCATGACAAAAAAGCCAGTCACGTTGACATGGGGCGGTAATAATTGCTCTACTGTGGAAATCTCTGGACTTGATATCGGATGGGGTCAGAGGATACCTTTAGAGTTTGATGACAAACAGGGTTCATGGATTCTTAAGAGGGAATTGCCGGAAGGACGATATGAATATAAGTACATCATTGATGGTGAATGGACACACAATAAAAATGAGCTTGTTGCCTCTGCAAACAAGGATGGTCATGTCAACAATTATGTTCAGATTGAGGATGATCCTAACAGTGTTAATGCCATCATAAGGAAGAGATTGATGGGTGATGATCCTGATCTAACAACGCACGAAAGACTTAAAATCAGACAGTTTCTTGAAGCTTATTGCAGTGATGAATGA

Protein Analysis

380

Amino Acids

42.29

Weight (kDa)

5.81

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-SAK PF22784 105 - 226 5.8e-06 Swiss Army Knife protein, DSP-PTPase phosphatase domain
DSPc PF00782 145 - 237 4.4e-11 Dual specificity phosphatase, catalytic domain
AMPK1_CBM PF16561 258 - 342 6.6e-19 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 440
AccI GTMKAC 1 cut(s) 432
AciI CCGC 6 cut(s) 41, 46, 99, 677, 735, 792
AclI AACGTT 1 cut(s) 700
AclWI GGATC 3 cut(s) 131, 1019, 1067
AcsI RAATTY 2 cut(s) 406, 464
AcuI CTGAAG 2 cut(s) 225, 323
AfaI GTAC 3 cut(s) 594, 654, 929
AfiI CCNNNNNNNGG 3 cut(s) 47, 718, 1050
AflII CTTAAG 1 cut(s) 890
AflIII ACRYGT 1 cut(s) 642
AgsI TTSAA 3 cut(s) 479, 671, 1121
AhlI ACTAGT 2 cut(s) 107, 125
AjnI CCWGG 2 cut(s) 22, 65
AleI CACNNNNGTG 1 cut(s) 486
AluBI AGCT 5 cut(s) 286, 364, 620, 967, 1124
AluI AGCT 5 cut(s) 286, 364, 620, 967, 1124
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 3 cut(s) 14, 185, 530
AlwI GGATC 3 cut(s) 131, 1019, 1067
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 2 cut(s) 252, 561
ApeKI GCWGC 2 cut(s) 43, 369
ApoI RAATTY 2 cut(s) 406, 464
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 953, 1079
AvaII GGWCC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 30, 381
BccI CCATC 5 cut(s) 831, 932, 983, 1052, 1057
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 2 cut(s) 24, 67
BciVI GTATCC 2 cut(s) 653, 843
BcoDI GTCTC 3 cut(s) 14, 185, 530
BcuI ACTAGT 2 cut(s) 107, 125
BfaI CTAG 2 cut(s) 108, 126
BfmI CTRYAG 1 cut(s) 274
BfrI CTTAAG 1 cut(s) 890
BfuI GTATCC 2 cut(s) 653, 843
BglII AGATCT 1 cut(s) 316
BisI GCNGC 3 cut(s) 41, 44, 370
BlsI GCNGC 3 cut(s) 42, 45, 371
Bme1390I CCNGG 2 cut(s) 24, 67
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 28, 175, 442
BmrFI CCNGG 2 cut(s) 24, 67
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 664
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 3 cut(s) 23, 65, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 718, 1050
Bse1I ACTGG 3 cut(s) 183, 723, 773
Bse3DI GCAATG 1 cut(s) 595
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 24, 67
BseDI CCNNGG 3 cut(s) 23, 65, 255
BseGI GGATG 3 cut(s) 842, 994, 1027
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 3 cut(s) 47, 718, 1050
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 3 cut(s) 180, 216, 683
BseNI ACTGG 3 cut(s) 183, 723, 773
BseRI GAGGAG 5 cut(s) 73, 76, 79, 82, 85
BseXI GCAGC 2 cut(s) 30, 381
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 2 cut(s) 254, 563
BshNI GGYRCC 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 2 cut(s) 529, 905
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 47, 718, 1050
BsmAI GTCTC 3 cut(s) 14, 185, 530
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 2 cut(s) 254, 563
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 6 cut(s) 41, 46, 99, 677, 735, 792
BspANI GGCC 2 cut(s) 254, 563
BspCNI CTCAG 3 cut(s) 181, 217, 684
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 3 cut(s) 28, 175, 442
BspPI GGATC 3 cut(s) 131, 1019, 1067
BspQI GCTCTTC 1 cut(s) 610
BspT107I GGYRCC 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 592
BsrI ACTGG 3 cut(s) 183, 723, 773
BssECI CCNNGG 3 cut(s) 23, 65, 255
BssMI GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 24, 67
Bst4CI ACNGT 4 cut(s) 8, 811, 1034, 1113
Bst6I CTCTTC 2 cut(s) 610, 1049
BstAFI CTTAAG 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 2 cut(s) 398, 489
BstDEI CTNAG 3 cut(s) 189, 225, 692
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 842, 994, 1027
BstKTI GATC 6 cut(s) 139, 319, 520, 1027, 1075, 1081
BstMAI GTCTC 3 cut(s) 14, 185, 530
BstMBI GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
BstMWI GCNNNNNNNGC 2 cut(s) 161, 260
BstNI CCWGG 2 cut(s) 24, 67
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 2 cut(s) 22, 65
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 30, 381
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuI GTATCC 2 cut(s) 653, 843
BsuRI GGCC 2 cut(s) 254, 563
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 3 cut(s) 842, 994, 1027
BtsI GCAGTG 2 cut(s) 600, 1138
BtsIMutI CAGTG 4 cut(s) 190, 600, 1039, 1138
Cac8I GCNNGC 2 cut(s) 398, 489
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 27
Csp6I GTAC 3 cut(s) 593, 653, 928
CviQI GTAC 3 cut(s) 593, 653, 928
DdeI CTNAG 3 cut(s) 189, 225, 692
DpnI GATC 6 cut(s) 138, 318, 519, 1026, 1074, 1080
DpnII GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
Eam1104I CTCTTC 2 cut(s) 610, 1049
EarI CTCTTC 2 cut(s) 610, 1049
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 1 cut(s) 832
Eco47I GGWCC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 2 cut(s) 225, 323
EcoICRI GAGCTC 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 22, 65
EcoRV GATATC 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 530
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 515
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 514
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 41, 44, 370
FokI GGATG 3 cut(s) 849, 1001, 1034
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 3 cut(s) 41, 44, 370
FspBI CTAG 2 cut(s) 108, 126
GluI GCNGC 3 cut(s) 41, 44, 370
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 2 cut(s) 254, 563
HapII CCGG 2 cut(s) 529, 905
HincII GTYRAC 4 cut(s) 358, 433, 783, 1000
HindII GTYRAC 4 cut(s) 358, 433, 783, 1000
HindIII AAGCTT 2 cut(s) 362, 1122
HinfI GANTC 4 cut(s) 16, 343, 509, 886
HpaII CCGG 2 cut(s) 529, 905
HphI GGTGA 2 cut(s) 953, 1079
Hpy166II GTNNAC 4 cut(s) 358, 433, 783, 1000
Hpy188I TCNGA 9 cut(s) 226, 321, 342, 412, 693, 836, 846, 1014, 1109
Hpy188III TCNNGA 7 cut(s) 13, 244, 268, 314, 822, 1076, 1118
Hpy8I GTNNAC 4 cut(s) 358, 433, 783, 1000
HpyAV CCTTC 4 cut(s) 88, 205, 347, 902
HpyCH4III ACNGT 4 cut(s) 8, 811, 1034, 1113
HpyCH4IV ACGT 4 cut(s) 310, 523, 700, 779
HpyCH4V TGCA 5 cut(s) 60, 400, 600, 980, 1131
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 260
HpyF3I CTNAG 3 cut(s) 189, 225, 692
HpySE526I ACGT 4 cut(s) 310, 523, 700, 779
Kzo9I GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 2 cut(s) 30, 381
LweI GCATC 1 cut(s) 664
MaeI CTAG 2 cut(s) 108, 126
MaeII ACGT 4 cut(s) 310, 523, 700, 779
MaeIII GTNAC 2 cut(s) 583, 775
MalI GATC 6 cut(s) 138, 318, 519, 1026, 1074, 1080
MboI GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
MboII GAAGA 5 cut(s) 11, 394, 627, 683, 1066
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 7 cut(s) 406, 464, 551, 683, 799, 899, 1003
MlyI GAGTC 1 cut(s) 337
MseI TTAA 3 cut(s) 891, 1038, 1101
MslI CAYNNNNRTG 3 cut(s) 377, 486, 758
MspA1I CMGCKG 1 cut(s) 43
MspCI CTTAAG 1 cut(s) 890
MspI CCGG 2 cut(s) 529, 905
MspR9I CCNGG 2 cut(s) 24, 67
MvaI CCWGG 2 cut(s) 24, 67
MwoI GCNNNNNNNGC 2 cut(s) 161, 260
NcoI CCATGG 1 cut(s) 255
NdeI CATATG 1 cut(s) 514
NdeII GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
NlaIV GGNNCC 3 cut(s) 28, 175, 442
NmuCI GTSAC 2 cut(s) 583, 775
NspI RCATGY 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 486
PagI TCATGA 1 cut(s) 243
PciI ACATGT 1 cut(s) 642
PciSI GCTCTTC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 16, 509, 886
PkrI GCNGC 3 cut(s) 42, 45, 371
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 27
PscI ACATGT 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 622
Psp1406I AACGTT 1 cut(s) 700
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 22, 65
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 22, 65
PspN4I GGNNCC 3 cut(s) 28, 175, 442
PspPI GGNCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 27
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 3 cut(s) 594, 654, 929
RsaNI GTAC 3 cut(s) 593, 653, 928
RseI CAYNNNNRTG 3 cut(s) 377, 486, 758
SacI GAGCTC 1 cut(s) 622
SalI GTCGAC 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 610
SaqAI TTAA 3 cut(s) 891, 1038, 1101
SatI GCNGC 3 cut(s) 41, 44, 370
Sau3AI GATC 6 cut(s) 136, 316, 517, 1024, 1072, 1078
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 2 cut(s) 24, 67
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 1 cut(s) 27
SmiMI CAYNNNNRTG 3 cut(s) 377, 486, 758
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
SpeI ACTAGT 2 cut(s) 107, 125
Sse9I AATT 7 cut(s) 406, 464, 551, 683, 799, 899, 1003
SsiI CCGC 6 cut(s) 41, 46, 99, 677, 735, 792
SspI AATATT 1 cut(s) 422
SspMI CTAG 2 cut(s) 108, 126
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 22, 65
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 4 cut(s) 8, 811, 1034, 1113
TaiI ACGT 4 cut(s) 313, 526, 703, 782
TaqI TCGA 1 cut(s) 432
TasI AATT 7 cut(s) 406, 464, 551, 683, 799, 899, 1003
TatI WGTACW 2 cut(s) 592, 927
TauI GCSGC 1 cut(s) 43
TfiI GAWTC 3 cut(s) 16, 509, 886
Tru1I TTAA 3 cut(s) 891, 1038, 1101
Tru9I TTAA 3 cut(s) 891, 1038, 1101
TscAI CASTG 4 cut(s) 190, 607, 1039, 1138
TseFI GTSAC 2 cut(s) 583, 775
TseI GCWGC 2 cut(s) 43, 369
Tsp45I GTSAC 2 cut(s) 583, 775
TspDTI ATGAA 7 cut(s) 17, 295, 308, 501, 693, 870, 933
TspRI CASTG 4 cut(s) 190, 607, 1039, 1138
Vha464I CTTAAG 1 cut(s) 890
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 406, 464
XceI RCATGY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 108, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.