RchiOBHm_Chr1g0349071

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003190
Physical Location & Seq
Reverse (-)
42098105 .. 42111687
13583 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4848 bp
ATGCCACCTAACACCTCACACCGCAAAACCGGTGAGTGTCTGCCTCTGCCTGAGAAGAAGAGCTTAAACCGGTGCCCCCCCCCCCCTCTCTCTCTCCATTTCTCTCGCCATTTCCGTCTCCCTCTCCACTGGTCCGGTCTCTCCGAAACCAAAACCATGTCGAAACCAAACCCTAAATCCCCTTACAATTCCCGCATAGCTCAGAACCAACTCATCGACTCCCTCACCTCCCACATCTCCCTCTACAATTCCCGCTCCCATTCCTCAGACCCCAAGCCCAACCCTAACCCTAGGTCCTCCATCCTCAAATGGTTCTCTTCTCTCTCCCTCCACCACCGCCAAGCTCACCTCACCGCCGTCGATCCCAAATTCGTCCGCCTCCTCCTCCAAATGCTCCGCAAGCTCCGCACGCACGGCCACGGCTCCTTCATCATCCTCCCCGACCTCCCCTCCGGCGACCTCCCCACGCTCTGCTTCAAGAGGTCCGGCGGGCTTTTGTCTAGGGTTGCCGAGTCGAACGAGTCGGAGAAGATGGTGTTCGAGTCGACTCGGTTGTTTTCGTCGAGGGAGGGAGAGAAGGTCGAGGAGTGTTCGTGTTCGGTGAGGGAGATTGATACGGTGACAGTGAGTGAGGAGTTGGTTGAGAATTTTGATAGGTTTGTGGAGGCTATGGATGATATATCAAATGGGGGCTTCCTGAGAGGTGAAGACAGTGATTTGGGGTCGGATTGGGTGGAATTGAAGTGGTTGAAGGACAAAGGGTATTACAGTATGGAAGCTTTCGTGGCGAACCGGTTAGAGGTTGCTCTGAGGCTTGCTTGGCTGAATTCGAATAATGCAAGGAAAAGAGGGGTGAAGTTGAAAGAGAAGATCAGCGCGGCCGGCGTGGCAGCCAATGTGTTTTGGAGGAAGAAGGGGTGTGTGGACTGGTGGGGGAATTTGGATGATGCGACGCGGAGCAATGTAGTGACTTCAGTGTTGGGGAAAGCAGCAAAACCGTTGATTCATGAGATTCTGAAGGGAACAAGTAGTGGGGTAGAGGATGAAATGTGGCTCGCCAACACAGGAATGGAGCAACCATTGAGGTACAACCATAATGTATCTATGCGCAAGACTGTTCCAAAACTTGTGGCTGACACAGAATTTGGGTCAAGCATTATCCCAGCTTCTCTTTCTGGGAAACCTGCTTCCTTAGCCGATGCTTTTAATAATCTCTTTGTGCTTCAGGATATCATAACGATGATATCATTATGTCGCCATAATGAATATGATAAAGGGAAATTCTTCTATACCACATTGAGTTCAATTTCTACCATTTCTGATTTTATACTAAGAAAAATACGAGGATTTCTCATGGTTCTTTTGCTTGACTGCACAAAACTTGAACTTTTAGCAGAGGGAAATGAAAAATGCTTACCTAAGAAACCCAAAGCAAAGCCTAGTGCTTCTAGCCGTAAAAGTAAGGGGAGGTCCCACAATATGAAAAGGCCAAATCCTGTTCCAGGGTCATGTACAGATGAATTTTTGTGTGAAACATCTGCTAAGGATCTTAGTACATTGGCTTATAAAGAGAAGGCAGATTCAGTGGAGTCCAAGACGACGCATACTATACATCAAGAGAAGGAGATTTCCAGAGAATCGTCATCATCAAAAGATGAAATGGAACATGCACAAGCATTGGCTGTTGGAAAAGTGCAGACAGCTGCGAGGAAGGGTAGGAAAGGAAAAGGCAAAAAGAAAATAACTGGCTTGAGGAGTGTTAATGATATGGAGAAATTTGATAGATCATTCATGGAAGCTTCTTCTTCTTCTTCAGTCATTCTTGACGCAACAAAGTCTGATCAGGTTTCTGGTGATACAGCCTACCAGAACATCGGATGTGATAATTCAGCTAGTTGTAACATCCCTTTGTCAAATTCAATTCCTTCTGGTTCTGCGAATGGACCTCTTAAAGATGAAGATGCCACCAAAAGCATTCAAGAGAATGATGGTATTGGTTCTAGTGCCAGTTTCTGTCATAAGGTTCTGGAAAGTTACCAGTGTTTAAATAATAATACTGAAATCCAAACAAAATGTTCTGGATCAGAAACTGAAGCCTGTAAGGTAGATGGAAACTTGATAAACTCTCCAGTGCCTGAGGTAGATGATAAAGTTTCCCATCATAAAGATATTGACTTGCAGGACAAGCGTGCTGTTAAGTCAGGTGCGAAAGAAGTTTTGCCAGCCAAAGAAATAAGAGCTTCTGATGTAAACGAGGAAGCTGTTCTGTTGCAGGACCAAGAGAGTGGAAATAATATATATCACACTAGGGCTCCAAGTTCTTTTGATTGCCCTCCTTATGAGTGGCCGGCTGTAGCTTGTGCCTATTTTCCACCTGTTAACTCACATCTCCCACCTGCCACCGATCGATTGCATCTGGATGTTGGTCATAACTGGCAGAATCACCGCCAGTCTTTTCTACCCACAATACATCAGGTGAGAAACTCGGCAATTGAAGGTGGGTGTAATCCAGTTCTGACTCGACCATTGCCAATGAGTATAGATTGGCCCCCAATGGTTCGAAGTGCTCGTAGAGTGGCTCCATACCGGACCTGTAATTATGATTCTGGGTTTGTCTCAAAAAAGCCGTGTACTTATCCACAGGGTTTTGCACACACTGTCCAAATAAATGCAACAACCATGGATGATGAAAGGAAGTATTCTTGGGATTGCGCAGACCTACCTGATCCAACAAAGGCATATGAACTAGTGGATGAATGTGACAGTCAATGGATATCTGAGGATGAAGTTGAGGTGCAAGCATTTTCTGGGGCAGATTATAATCAATACTTTGGTGGTGGTGTGATGTACTGGAATCCTTCTGATCATACCGGGACAGTTTTCTCTCGCCCTCCTTCCCTTAGTTCTGATGATAGCTCATGGGCTTGGCGTGAAGCTGACATAAATAGAGCTGTTGATGATATGGTTGCATTTTCTTCTCCGTACAGTACAAATGGTTTGACTTCACCTACTGCTTCCTTCTGTTCTCCTTTTGATCCTTTGGGATCAGGAAACCAGCCTCTTGGATATGTTATGTCAGGAAATGAAGTACCAGGCAAGGTGCTGCATCCCTCATCAACAATGGGAGACACAGTAGTAGATGACGAATCGTCTGGATCTTTGGCTGACGTAACTGGTGATATTGAAGGGAAGACAGGCGATTCATTGCCTTACCCCATGTTGCGGCCAATCATCATATCAAGATCAAGAGACTTTAAGCGCAGCCATGATCGTAAAAGCCCATGTGTTCCTCCTACTATGCGTGATCGACCTAGGATAAAGCGGCCACCGTCGCCGGTGGTGCTTTCTGTCCCACGCGCTCCACGACCACCTCCACCATCTTCTGTGAGTGACTCGAGGAAACACAGGGGATTTCCAACTGTTCGTTCTGGTAGCTCTAGCCCAAGGCACTGGGGTATGAGAGGTTGGTTCCATGATGGAGCTAACTTGGAGGAAGCTTGTTTACGCATGGATGGTGCTGAAGTTGTTTGGCCATTTAGAAATAATATTTCAGGCCCCCCATTGATTCAGCCTCTTCCTGCACCTCTGTTACAGGATCGACTGATTGCTATTTCTCAACTAGCACGTGATCAGGAACATCCGGATGTTGCATTTCCCCTTCAGCCGCCTGAGTTACATAACTGTCCAACACGGAAGGCAAATCTCTCTCTGATGCACAGCCTTGTGCATGATGAGATTGAATTTTTTTGCAAGAAGGTTGCTACAGAGAATATGTCTCGGAAGCCCTACATCAATTGGGCTGTCAAACGAGTTACACGGTCTCTCCAGGTGCTTTGGCCCAGATCCAGGACAAATATATTTGGTTCAGTTGCAAACGGTTTGTCCCTTCCAACTAGTGATGTGGACCTTGTGGTTTCTCTACCTCCTGTTAGGAATTTGGAACCCATTAAAGAAGCTGGGATATTGGAGGGTCGAAATGGTATTAAAGAGACATGCCTTCAGCATGCTGCCAGGTATCTCGTCAATCAGGATTGGGTTAAAAATGATTCTCTTAAGACCGTGGAAAATACAGCTATACCAATTATAATGCTTGTGGTGGAAGTTCCCAATGATCTAATTGCCTCGTCTTCGTCTAACGTGCAATCACCAAAAGAAGAGGCACCACACAGTACTGGTGAACCAGACTGTAATGTGCACTCCAGTGGGGTTATTTTAGAAGAATCTGCTATGCCTAAGTGCCCCCAGATAACTTGTGATGCTACAAAGGATTCAGTATCAATTCGCATTGACATCAGTTTCAAATCTCCATCTCATACAGGATTTCAAACTACACAGCTGGTTAAAGATTTAACTGAGCAGTTTCCAGCTGCTACACCCCTTGCTTTGGTACTGAAACAGTTCTTGGCGGATCGTAGTCTGGATCAGTCTTATTCTGGTGGCTTGAGTTCCTACTGCTTGGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATCATCTTAGCCGACCTATCAACCAAAACTTTGGAAACCTTCTGATGAATTTTCTTTATTTTTTTGGGAATGTTTTTGATCCTCGGCAAATGCGTATTTCCGTACAGGGAAGTGGTGTTTATATAAAGAGGGAAAGAGGTTGCAGCATCGACCCCATACACATTGATGATCCCCTTTATCCGACTAATAATGTCGGACGCAATTGCTTTCGGATACATCAGTGTATCAAGGCGTTTTCAGAAGCTTATTCTATTTTGGAGAATGAGCTTGCTTCTCTTCCTAATGATGATGATGCATCTTCAAGTCCCCCATATAGACTGCTTCCTAAAATTATCCCGAGTATTGATCTAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

1615

Amino Acids

179.1

Weight (kDa)

6.87

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 1267 - 1363 2e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1566, 2820, 4085
AarI CACCTGC 1 cut(s) 2404
Acc16I TGCGCA 2 cut(s) 1109, 2713
Acc36I ACCTGC 2 cut(s) 1192, 2404
AccB1I GGYRCC 2 cut(s) 72, 4159
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 545
AccII CGCG 3 cut(s) 878, 955, 3357
AccIII TCCGGA 1 cut(s) 3640
AcoI YGGCCR 6 cut(s) 415, 879, 2345, 3224, 3323, 3530
AcuI CTGAAG 8 cut(s) 957, 1037, 1208, 1797, 2112, 3540, 3644, 3983
AcvI CACGTG 1 cut(s) 3626
AfiI CCNNNNNNNGG 5 cut(s) 799, 1502, 3222, 3846, 4384
AflII CTTAAG 1 cut(s) 4052
AgeI ACCGGT 3 cut(s) 29, 69, 792
AhdI GACNNNNNGTC 1 cut(s) 3148
AhlI ACTAGT 2 cut(s) 2746, 3893
AjnI CCWGG 5 cut(s) 1501, 3091, 3825, 3845, 4010
AjuI GAANNNNNNNTTGG 4 cut(s) 3409, 3441, 4385, 4417
AleI CACNNNNGTG 1 cut(s) 4200
Alw21I GWGCWC 2 cut(s) 2569, 4197
Alw26I GTCTC 8 cut(s) 122, 143, 2620, 3120, 3243, 3780, 3825, 3983
Alw44I GTGCAC 1 cut(s) 4193
AlwNI CAGNNNCTG 3 cut(s) 2013, 2090, 2135
Ama87I CYCGRG 2 cut(s) 3394, 4828
Aor13HI TCCGGA 1 cut(s) 3640
ApaLI GTGCAC 1 cut(s) 4193
ApeKI GCWGC 8 cut(s) 890, 989, 1703, 3103, 3261, 4007, 4367, 4635
ArsI GACNNNNNNTTYG 4 cut(s) 1127, 1159, 3866, 3898
AsiGI ACCGGT 3 cut(s) 29, 69, 792
Asp700I GAANNNNTTC 3 cut(s) 1283, 2262, 4397
AspA2I CCTAGG 2 cut(s) 290, 3311
AspLEI GCGC 5 cut(s) 878, 1110, 2714, 3261, 3359
AsuC2I CCSGG 1 cut(s) 2872
AsuII TTCGAA 2 cut(s) 830, 2560
AvaI CYCGRG 2 cut(s) 3394, 4828
AvaII GGWCC 8 cut(s) 132, 294, 483, 1470, 1943, 2275, 2589, 3904
AvrII CCTAGG 2 cut(s) 290, 3311
AxyI CCTNAGG 1 cut(s) 2136
BaeGI GKGCMC 3 cut(s) 77, 4197, 4241
BaeI ACNNNNGTAYC 2 cut(s) 4697, 4730
BalI TGGCCA 1 cut(s) 3532
BanI GGYRCC 2 cut(s) 72, 4159
BanII GRGCYC 1 cut(s) 2314
BarI GAAGNNNNNNTAC 2 cut(s) 3486, 3518
BbrPI CACGTG 1 cut(s) 3626
BbsI GAAGAC 3 cut(s) 714, 3197, 4119
Bbv12I GWGCWC 2 cut(s) 2569, 4197
BbvI GCAGC 8 cut(s) 902, 1001, 1690, 3090, 3273, 3994, 4354, 4647
BccI CCATC 9 cut(s) 308, 526, 1982, 2102, 2166, 3385, 3470, 3506, 4315
BceAI ACGGC 5 cut(s) 341, 430, 436, 1437, 2611
BciT130I CCWGG 5 cut(s) 1503, 3093, 3827, 3847, 4012
BciVI GTATCC 1 cut(s) 4698
BclI TGATCA 3 cut(s) 1840, 2863, 3628
BcnI CCSGG 1 cut(s) 2872
BcoDI GTCTC 8 cut(s) 122, 143, 2620, 3120, 3243, 3780, 3825, 3983
BcuI ACTAGT 2 cut(s) 2746, 3893
BfmI CTRYAG 2 cut(s) 2352, 3762
BfrI CTTAAG 1 cut(s) 4052
BfuAI ACCTGC 2 cut(s) 1192, 2404
BfuI GTATCC 1 cut(s) 4698
BglI GCCNNNNNGGC 1 cut(s) 887
BlnI CCTAGG 2 cut(s) 290, 3311
BmcAI AGTACT 1 cut(s) 4171
Bme1390I CCNGG 6 cut(s) 1503, 2872, 3093, 3827, 3847, 4012
Bme18I GGWCC 8 cut(s) 132, 294, 483, 1470, 1943, 2275, 2589, 3904
BmeRI GACNNNNNGTC 1 cut(s) 3148
BmeT110I CYCGRG 2 cut(s) 3394, 4828
BmrFI CCNGG 6 cut(s) 1503, 2872, 3093, 3827, 3847, 4012
BmrI ACTGGG 1 cut(s) 3460
BmuI ACTGGG 1 cut(s) 3460
BpiI GAAGAC 3 cut(s) 714, 3197, 4119
BplI GAGNNNNNCTC 2 cut(s) 1335, 1367
BpmI CTGGAG 4 cut(s) 2112, 3809, 4183, 4466
Bpu10I CCTNAGC 2 cut(s) 1192, 1542
Bpu14I TTCGAA 2 cut(s) 830, 2560
BpuEI CTTGAG 2 cut(s) 1771, 4462
BpuMI CCSGG 1 cut(s) 2872
Bsa29I ATCGAT 1 cut(s) 2407
BsaAI YACGTR 1 cut(s) 3626
BsaBI GATNNNNATC 1 cut(s) 2820
BsaI GGTCTC 2 cut(s) 143, 3825
BsaJI CCNNGG 8 cut(s) 290, 418, 1502, 2679, 3311, 3443, 4059, 4574
BsaWI WCCGGW 6 cut(s) 29, 69, 134, 792, 2586, 3640
Bsc4I CCNNNNNNNGG 5 cut(s) 799, 1502, 3222, 3846, 4384
Bse118I RCCGGY 6 cut(s) 29, 69, 792, 881, 2347, 3334
Bse21I CCTNAGG 1 cut(s) 2136
Bse3DI GCAATG 3 cut(s) 967, 2525, 3203
Bse8I GATNNNNATC 1 cut(s) 2820
BseAI TCCGGA 1 cut(s) 3640
BseBI CCWGG 5 cut(s) 1503, 3093, 3827, 3847, 4012
BseCI ATCGAT 1 cut(s) 2407
BseDI CCNNGG 8 cut(s) 290, 418, 1502, 2679, 3311, 3443, 4059, 4574
BseJI GATNNNNATC 1 cut(s) 2820
BseLI CCNNNNNNNGG 5 cut(s) 799, 1502, 3222, 3846, 4384
BseMI GCAATG 3 cut(s) 967, 2525, 3203
BseMII CTCAG 9 cut(s) 42, 215, 279, 689, 800, 2127, 2769, 3660, 4344
BseRI GAGGAG 5 cut(s) 371, 374, 599, 647, 1768
BseSI GKGCMC 3 cut(s) 77, 4197, 4241
BseX3I CGGCCG 1 cut(s) 879
BseXI GCAGC 8 cut(s) 902, 1001, 1690, 3090, 3273, 3994, 4354, 4647
BseYI CCCAGC 2 cut(s) 1162, 3956
BsgI GTGCAG 3 cut(s) 1357, 1715, 3564
Bsh1236I CGCG 3 cut(s) 878, 955, 3357
Bsh1285I CGRYCG 2 cut(s) 882, 2407
BshNI GGYRCC 2 cut(s) 72, 4159
BshTI ACCGGT 3 cut(s) 29, 69, 792
BshVI ATCGAT 1 cut(s) 2407
BsiEI CGRYCG 2 cut(s) 882, 2407
BsiHKAI GWGCWC 2 cut(s) 2569, 4197
BsiHKCI CYCGRG 2 cut(s) 3394, 4828
BslFI GGGAC 5 cut(s) 1456, 2887, 3335, 3868, 4782
BslI CCNNNNNNNGG 5 cut(s) 799, 1502, 3222, 3846, 4384
BsmAI GTCTC 8 cut(s) 122, 143, 2620, 3120, 3243, 3780, 3825, 3983
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 5 cut(s) 1456, 2887, 3335, 3868, 4782
BsmI GAATGC 1 cut(s) 1974
Bso31I GGTCTC 2 cut(s) 143, 3825
BsoBI CYCGRG 2 cut(s) 3394, 4828
Bsp119I TTCGAA 2 cut(s) 830, 2560
Bsp1286I GDGCHC 5 cut(s) 77, 2314, 2569, 4197, 4241
Bsp13I TCCGGA 1 cut(s) 3640
Bsp1407I TGTACA 1 cut(s) 1511
Bsp19I CCATGG 1 cut(s) 2679
BspCNI CTCAG 9 cut(s) 43, 214, 278, 690, 801, 2128, 2770, 3661, 4345
BspDI ATCGAT 1 cut(s) 2407
BspEI TCCGGA 1 cut(s) 3640
BspFNI CGCG 3 cut(s) 878, 955, 3357
BspHI TCATGA 1 cut(s) 1006
BspMI ACCTGC 2 cut(s) 1192, 2404
BspQI GCTCTTC 1 cut(s) 53
BspT104I TTCGAA 2 cut(s) 830, 2560
BspT107I GGYRCC 2 cut(s) 72, 4159
BspTI CTTAAG 1 cut(s) 4052
BspTNI GGTCTC 2 cut(s) 143, 3825
BsrBI CCGCTC 1 cut(s) 255
BsrDI GCAATG 3 cut(s) 967, 2525, 3203
BsrFI RCCGGY 6 cut(s) 29, 69, 792, 881, 2347, 3334
BsrGI TGTACA 1 cut(s) 1511
BssAI RCCGGY 6 cut(s) 29, 69, 792, 881, 2347, 3334
BssECI CCNNGG 8 cut(s) 290, 418, 1502, 2679, 3311, 3443, 4059, 4574
BssT1I CCWWGG 4 cut(s) 290, 2679, 3311, 3443
Bst2UI CCWGG 5 cut(s) 1503, 3093, 3827, 3847, 4012
Bst6I CTCTTC 5 cut(s) 53, 322, 3579, 4149, 4773
BstAFI CTTAAG 1 cut(s) 4052
BstAUI TGTACA 1 cut(s) 1511
BstBAI YACGTR 1 cut(s) 3626
BstBI TTCGAA 2 cut(s) 830, 2560
BstDSI CCRYGG 3 cut(s) 418, 2679, 4059
BstENI CCTNNNNNAGG 1 cut(s) 1500
BstFNI CGCG 3 cut(s) 878, 955, 3357
BstHHI GCGC 5 cut(s) 878, 1110, 2714, 3261, 3359
BstMAI GTCTC 8 cut(s) 122, 143, 2620, 3120, 3243, 3780, 3825, 3983
BstMCI CGRYCG 2 cut(s) 882, 2407
BstNI CCWGG 5 cut(s) 1503, 3093, 3827, 3847, 4012
BstNSI RCATGY 3 cut(s) 1670, 3996, 4007
BstSCI CCNGG 6 cut(s) 1501, 2870, 3091, 3825, 3845, 4010
BstSFI CTRYAG 2 cut(s) 2352, 3762
BstSLI GKGCMC 3 cut(s) 77, 4197, 4241
BstUI CGCG 3 cut(s) 878, 955, 3357
BstV1I GCAGC 8 cut(s) 902, 1001, 1690, 3090, 3273, 3994, 4354, 4647
BstV2I GAAGAC 3 cut(s) 714, 3197, 4119
BstX2I RGATCY 3 cut(s) 1546, 3155, 3842
BstXI CCANNNNNNTGG 4 cut(s) 2285, 3062, 3450, 4523
BstYI RGATCY 3 cut(s) 1546, 3155, 3842
BstZI CGGCCG 1 cut(s) 879
Bsu15I ATCGAT 1 cut(s) 2407
Bsu36I CCTNAGG 1 cut(s) 2136
BsuI GTATCC 1 cut(s) 4698
BsuTUI ATCGAT 1 cut(s) 2407
BtgI CCRYGG 3 cut(s) 418, 2679, 4059
BveI ACCTGC 2 cut(s) 1192, 2404
CaiI CAGNNNCTG 3 cut(s) 2013, 2090, 2135
CciI TCATGA 1 cut(s) 1006
CfoI GCGC 5 cut(s) 878, 1110, 2714, 3261, 3359
Cfr10I RCCGGY 6 cut(s) 29, 69, 792, 881, 2347, 3334
ClaI ATCGAT 1 cut(s) 2407
CseI GACGC 4 cut(s) 961, 1609, 1835, 4698
CspAI ACCGGT 3 cut(s) 29, 69, 792
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
DraI TTTAAA 1 cut(s) 2046
DriI GACNNNNNGTC 1 cut(s) 3148
EaeI YGGCCR 6 cut(s) 415, 879, 2345, 3224, 3323, 3530
EagI CGGCCG 1 cut(s) 879
Eam1104I CTCTTC 5 cut(s) 53, 322, 3579, 4149, 4773
Eam1105I GACNNNNNGTC 1 cut(s) 3148
EarI CTCTTC 5 cut(s) 53, 322, 3579, 4149, 4773
EciI GGCGGA 2 cut(s) 365, 4421
EclXI CGGCCG 1 cut(s) 879
Eco130I CCWWGG 4 cut(s) 290, 2679, 3311, 3443
Eco24I GRGCYC 1 cut(s) 2314
Eco31I GGTCTC 2 cut(s) 143, 3825
Eco32I GATATC 3 cut(s) 1231, 1245, 2775
Eco47I GGWCC 8 cut(s) 132, 294, 483, 1470, 1943, 2275, 2589, 3904
Eco52I CGGCCG 1 cut(s) 879
Eco57I CTGAAG 8 cut(s) 957, 1037, 1208, 1797, 2112, 3540, 3644, 3983
Eco72I CACGTG 1 cut(s) 3626
Eco81I CCTNAGG 1 cut(s) 2136
Eco88I CYCGRG 2 cut(s) 3394, 4828
EcoNI CCTNNNNNAGG 1 cut(s) 1500
EcoO109I RGGNCCY 3 cut(s) 294, 1470, 3554
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 5 cut(s) 1501, 3091, 3825, 3845, 4010
EcoRV GATATC 3 cut(s) 1231, 1245, 2775
EcoT14I CCWWGG 4 cut(s) 290, 2679, 3311, 3443
EcoT22I ATGCAT 1 cut(s) 4789
EcoT38I GRGCYC 1 cut(s) 2314
ErhI CCWWGG 4 cut(s) 290, 2679, 3311, 3443
Esp3I CGTCTC 1 cut(s) 122
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 5 cut(s) 1456, 2887, 3335, 3868, 4782
FauI CCCGC 3 cut(s) 200, 260, 482
FauNDI CATATG 1 cut(s) 2740
FbaI TGATCA 3 cut(s) 1840, 2863, 3628
FblI GTMKAC 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 2314
FspI TGCGCA 2 cut(s) 1109, 2713
GlaI GCGC 5 cut(s) 877, 1109, 2713, 3260, 3358
GsaI CCCAGC 2 cut(s) 1166, 3960
GsuI CTGGAG 4 cut(s) 2112, 3809, 4183, 4466
HgaI GACGC 4 cut(s) 961, 1609, 1835, 4698
HhaI GCGC 5 cut(s) 878, 1110, 2714, 3261, 3359
Hin6I GCGC 5 cut(s) 876, 1108, 2712, 3259, 3357
HinP1I GCGC 5 cut(s) 876, 1108, 2712, 3259, 3357
HincII GTYRAC 2 cut(s) 546, 2380
HindII GTYRAC 2 cut(s) 546, 2380
HindIII AAGCTT 4 cut(s) 777, 1797, 3495, 4734
HpaI GTTAAC 1 cut(s) 2380
Hpy166II GTNNAC 9 cut(s) 546, 925, 2251, 2380, 2631, 3503, 3904, 4178, 4195
Hpy8I GTNNAC 9 cut(s) 546, 925, 2251, 2380, 2631, 3503, 3904, 4178, 4195
Hpy99I CGWCG 5 cut(s) 362, 565, 955, 1603, 3334
HpyCH4IV ACGT 3 cut(s) 3168, 3625, 4137
HpySE526I ACGT 3 cut(s) 3168, 3625, 4137
HspAI GCGC 5 cut(s) 876, 1108, 2712, 3259, 3357
Kpn2I TCCGGA 1 cut(s) 3640
KroI GCCGGC 2 cut(s) 881, 2347
KroNI GCCGGC 2 cut(s) 883, 2349
Ksp22I TGATCA 3 cut(s) 1840, 2863, 3628
KspAI GTTAAC 1 cut(s) 2380
LguI GCTCTTC 1 cut(s) 53
Lsp1109I GCAGC 8 cut(s) 902, 1001, 1690, 3090, 3273, 3994, 4354, 4647
MaeII ACGT 3 cut(s) 3168, 3625, 4137
MbiI CCGCTC 1 cut(s) 255
MfeI CAATTG 3 cut(s) 2490, 3793, 4693
MflI RGATCY 3 cut(s) 1546, 3155, 3842
MhlI GDGCHC 5 cut(s) 77, 2314, 2569, 4197, 4241
MlsI TGGCCA 1 cut(s) 3532
MluNI TGGCCA 1 cut(s) 3532
MlyI GAGTC 8 cut(s) 212, 521, 530, 541, 551, 1598, 2512, 3386
MmeI TCCRAC 9 cut(s) 504, 705, 1666, 2753, 3440, 3710, 3914, 4666, 4697
Mox20I TGGCCA 1 cut(s) 3532
Mph1103I ATGCAT 1 cut(s) 4789
MroI TCCGGA 1 cut(s) 3640
MroNI GCCGGC 2 cut(s) 881, 2347
MroXI GAANNNNTTC 3 cut(s) 1283, 2262, 4397
MscI TGGCCA 1 cut(s) 3532
MslI CAYNNNNRTG 6 cut(s) 1067, 1238, 2418, 3642, 4200, 4656
Msp20I TGGCCA 1 cut(s) 3532
MspA1I CMGCKG 3 cut(s) 1703, 4336, 4367
MspCI CTTAAG 1 cut(s) 4052
MspR9I CCNGG 6 cut(s) 1503, 2872, 3093, 3827, 3847, 4012
MunI CAATTG 3 cut(s) 2490, 3793, 4693
Mva1269I GAATGC 1 cut(s) 1974
MvaI CCWGG 5 cut(s) 1503, 3093, 3827, 3847, 4012
MvnI CGCG 3 cut(s) 878, 955, 3357
NaeI GCCGGC 2 cut(s) 883, 2349
NciI CCSGG 1 cut(s) 2872
NcoI CCATGG 1 cut(s) 2679
NdeI CATATG 1 cut(s) 2740
NgoMIV GCCGGC 2 cut(s) 881, 2347
NmeAIII GCCGAG 3 cut(s) 535, 2465, 4555
NmuCI GTSAC 4 cut(s) 619, 967, 2759, 3389
NsbI TGCGCA 2 cut(s) 1109, 2713
NsiI ATGCAT 1 cut(s) 4789
NspI RCATGY 3 cut(s) 1670, 3996, 4007
NspV TTCGAA 2 cut(s) 830, 2560
OliI CACNNNNGTG 1 cut(s) 4200
PaeI GCATGC 1 cut(s) 4007
PaeR7I CTCGAG 1 cut(s) 3394
PagI TCATGA 1 cut(s) 1006
PaqCI CACCTGC 1 cut(s) 2404
PciSI GCTCTTC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 4 cut(s) 112, 2566, 3361, 4582
PctI GAATGC 1 cut(s) 1974
PdiI GCCGGC 2 cut(s) 883, 2349
PdmI GAANNNNTTC 3 cut(s) 1283, 2262, 4397
PfoI TCCNGGA 1 cut(s) 3845
PinAI ACCGGT 3 cut(s) 29, 69, 792
Ple19I CGATCG 1 cut(s) 2407
PleI GAGTC 8 cut(s) 212, 520, 529, 541, 550, 1597, 2512, 3386
PmaCI CACGTG 1 cut(s) 3626
PmlI CACGTG 1 cut(s) 3626
PpsI GAGTC 8 cut(s) 212, 520, 529, 541, 550, 1597, 2512, 3386
Ppu21I YACGTR 1 cut(s) 3626
PpuMI RGGWCCY 2 cut(s) 294, 1470
PsiI TTATAA 3 cut(s) 1566, 2820, 4085
Psp5II RGGWCCY 2 cut(s) 294, 1470
Psp6I CCWGG 5 cut(s) 1501, 3091, 3825, 3845, 4010
PspCI CACGTG 1 cut(s) 3626
PspFI CCCAGC 2 cut(s) 1162, 3956
PspGI CCWGG 5 cut(s) 1501, 3091, 3825, 3845, 4010
PspPPI RGGWCCY 2 cut(s) 294, 1470
PspXI VCTCGAGB 1 cut(s) 3394
PstNI CAGNNNCTG 3 cut(s) 2013, 2090, 2135
PsuI RGATCY 3 cut(s) 1546, 3155, 3842
PvuI CGATCG 1 cut(s) 2407
PvuII CAGCTG 3 cut(s) 1703, 4336, 4367
RseI CAYNNNNRTG 6 cut(s) 1067, 1238, 2418, 3642, 4200, 4656
SalI GTCGAC 1 cut(s) 544
SapI GCTCTTC 1 cut(s) 53
ScaI AGTACT 1 cut(s) 4171
SchI GAGTC 8 cut(s) 212, 521, 530, 541, 551, 1598, 2512, 3386
ScrFI CCNGG 6 cut(s) 1503, 2872, 3093, 3827, 3847, 4012
SduI GDGCHC 5 cut(s) 77, 2314, 2569, 4197, 4241
SfcI CTRYAG 2 cut(s) 2352, 3762
Sfr274I CTCGAG 1 cut(s) 3394
SfuI TTCGAA 2 cut(s) 830, 2560
SgrAI CRCCGGYG 1 cut(s) 3334
SinI GGWCC 8 cut(s) 132, 294, 483, 1470, 1943, 2275, 2589, 3904
SlaI CTCGAG 1 cut(s) 3394
SmiMI CAYNNNNRTG 6 cut(s) 1067, 1238, 2418, 3642, 4200, 4656
SmlI CTYRAG 4 cut(s) 1750, 3394, 4052, 4441
SmoI CTYRAG 4 cut(s) 1750, 3394, 4052, 4441
SpeI ACTAGT 2 cut(s) 2746, 3893
SphI GCATGC 1 cut(s) 4007
SspI AATATT 1 cut(s) 3547
StyD4I CCNGG 6 cut(s) 1501, 2870, 3091, 3825, 3845, 4010
StyI CCWWGG 4 cut(s) 290, 2679, 3311, 3443
TaiI ACGT 3 cut(s) 3171, 3628, 4140
TatI WGTACW 6 cut(s) 1511, 1553, 2630, 2847, 2987, 4169
TauI GCSGC 4 cut(s) 881, 3226, 3325, 3667
TseFI GTSAC 4 cut(s) 619, 967, 2759, 3389
TseI GCWGC 8 cut(s) 890, 989, 1703, 3103, 3261, 4007, 4367, 4635
Tsp45I GTSAC 4 cut(s) 619, 967, 2759, 3389
TspGWI ACGGA 4 cut(s) 104, 2970, 3706, 4582
Vha464I CTTAAG 1 cut(s) 4052
VneI GTGCAC 1 cut(s) 4193
VpaK11BI GGWCC 8 cut(s) 132, 294, 483, 1470, 1943, 2275, 2589, 3904
XagI CCTNNNNNAGG 1 cut(s) 1500
XceI RCATGY 3 cut(s) 1670, 3996, 4007
XcmI CCANNNNNNNNNTGG 1 cut(s) 1066
XhoI CTCGAG 1 cut(s) 3394
XmaJI CCTAGG 2 cut(s) 290, 3311
XmiI GTMKAC 1 cut(s) 545
XmnI GAANNNNTTC 3 cut(s) 1283, 2262, 4397
ZrmI AGTACT 1 cut(s) 4171
Zsp2I ATGCAT 1 cut(s) 4789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.