RchiOBHm_Chr1g0349511

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010300
Physical Location & Seq
Forward (+)
42599262 .. 42601450
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGAGCAACCTTCACTTACACTCTTCCATTTCCAAAACAATCTTAGCCCTGCGAATCTCTTCCCTTTTCTTTCTCTTTTCTTCACTCAATACCACCCATGGCTCCCCAATCAAAGCTAACATCTTCATCTATGCAGGCTGCTCACAAGAAAAGTACCAACCAAACTCACCCTTTGAAGCTAACCTCAACTCTTTCCTAACCTCAGTTGTTAGCTCATCCTCTCAAGCCTCTTACAACGCCTTTTCTGTCGGAAACGGAACCTCAACATCACCAGAAGGTGCCATTTATGGCTTGTACCAGTGCAGAGGTGACTTAAAGATTTTTGACTGTTCGAAATGCATCGAAAGCGCGGTGAATCAAGTCACTCTAGTCTGTCCCTACTCTTATGGCGCCTCTTTGCAGTTACAAGGTTGTTATGTTAGATACGAGCATGCTGAGTTTTTGGGCAAGCTTGATACAAGTTTGAGGTACAAGAAATGTAGTAAGACTGTACATGAAGATGTTGAGTTCTTTCGGAGAAGAGATGACGTGCTAGCTGACTTGCAAGGGACTAGTGGATTTAGGGTTAGTAGTTCAGGCTTGGTTGAGGGTTTTGCGCAGTGTTTGGGGGATTTGAGCCAAAACGACTGCTCTTCTTGCGTCGCAGATGCTGTGGGAAAGCTGAAGAGTTTGTGTGGGTCAGCAGCTGCGGTAGATGTGTTCTTGGCTCAGTGTTATGCTAGGTACTGGGCTTCTGGCTACTACGATATCTCAGATTCCTCTAACGAAGACCAAGTGGGAAAGACTGTGGCCATTATCGTGGGGGTGGTAGCAAGTCTAGCTATTCTCATTGTTCTTCTATCAGTTTGCCGGAAAACCATGGGTCGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

290

Amino Acids

31.42

Weight (kDa)

6.91

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 47 - 144 1.9e-20 Salt stress response/antifungal
Stress-antifung PF01657 160 - 242 9.5e-11 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60720 AT3G60720 AT3G60720
fragaria_vesca FvH4_7g11310 FvH4_7g11310
malus_domestica MD02G1208100.v1.1 MD07G1117900.v1.1
prunus_persica Prupe.2G140900_v2.0.a1
pyrus_communis pycom02g17030 pycom07g10630
rosa_chinensis RchiOBHm_Chr1g0349511
rosa_laevigata RLG00000028545
rosa_multiflora Rmu_co8400581.1_g000001 Rmu_sc0002495.1_g000015
rosa_roxburghii Rroxscaffold_4G00305500
rosa_rugosa Rorug01G0204200
rosa_samantha Rh1AG221500 Rh1BG187600 Rh1CG206400 Rh1DG216900
rosa_wichuraiana Rw1G018830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 597
AccB1I GGYRCC 2 cut(s) 278, 389
AccII CGCG 1 cut(s) 350
AciI CCGC 2 cut(s) 350, 689
AcoI YGGCCR 1 cut(s) 789
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 5 cut(s) 155, 296, 470, 492, 725
AgsI TTSAA 1 cut(s) 176
AhlI ACTAGT 1 cut(s) 551
AjiI CACGTC 1 cut(s) 529
AluBI AGCT 8 cut(s) 116, 179, 213, 451, 536, 661, 686, 821
AluI AGCT 8 cut(s) 116, 179, 213, 451, 536, 661, 686, 821
AlwNI CAGNNNCTG 2 cut(s) 650, 686
AoxI GGCC 1 cut(s) 789
ApeKI GCWGC 3 cut(s) 138, 683, 686
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 3 cut(s) 350, 392, 598
AsuHPI GGTGA 4 cut(s) 159, 261, 320, 364
AsuII TTCGAA 1 cut(s) 332
AsuNHI GCTAGC 1 cut(s) 532
BalI TGGCCA 1 cut(s) 791
BanI GGYRCC 2 cut(s) 278, 389
BbsI GAAGAC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 125, 673, 695
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 5 cut(s) 368, 533, 552, 720, 818
BfoI RGCGCY 1 cut(s) 393
BisI GCNGC 3 cut(s) 139, 684, 687
BlsI GCNGC 3 cut(s) 140, 685, 688
BmgBI CACGTC 1 cut(s) 529
BmiI GGNNCC 4 cut(s) 103, 259, 280, 391
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 348, 637
BmtI GCTAGC 1 cut(s) 536
BmuI ACTGGG 1 cut(s) 736
BpiI GAAGAC 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 97, 858
Bse1I ACTGG 2 cut(s) 298, 731
BseDI CCNNGG 2 cut(s) 97, 858
BseGI GGATG 1 cut(s) 215
BseMII CTCAG 4 cut(s) 216, 426, 722, 765
BseNI ACTGG 2 cut(s) 298, 731
BseXI GCAGC 3 cut(s) 125, 673, 695
BsgI GTGCAG 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 350
BshFI GGCC 1 cut(s) 791
BshNI GGYRCC 2 cut(s) 278, 389
BsiSI CCGG 1 cut(s) 850
BslFI GGGAC 2 cut(s) 360, 562
BsmFI GGGAC 2 cut(s) 360, 562
BsnI GGCC 1 cut(s) 791
Bsp119I TTCGAA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 490
Bsp19I CCATGG 2 cut(s) 97, 858
BspACI CCGC 2 cut(s) 350, 689
BspANI GGCC 1 cut(s) 791
BspCNI CTCAG 4 cut(s) 215, 427, 721, 764
BspFNI CGCG 1 cut(s) 350
BspLI GGNNCC 4 cut(s) 103, 259, 280, 391
BspOI GCTAGC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 637
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 2 cut(s) 278, 389
BsrGI TGTACA 1 cut(s) 490
BsrI ACTGG 2 cut(s) 298, 731
BssECI CCNNGG 2 cut(s) 97, 858
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 2 cut(s) 97, 858
Bst4CI ACNGT 3 cut(s) 329, 490, 787
Bst6I CTCTTC 5 cut(s) 28, 64, 514, 637, 659
BstACI GRCGYC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 136, 432, 449, 534
BstDEI CTNAG 5 cut(s) 43, 202, 435, 708, 751
BstDSI CCRYGG 2 cut(s) 97, 858
BstF5I GGATG 1 cut(s) 215
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 3 cut(s) 350, 392, 598
BstMWI GCNNNNNNNGC 3 cut(s) 345, 636, 818
BstNSI RCATGY 1 cut(s) 434
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 3 cut(s) 125, 673, 695
BstV2I GAAGAC 1 cut(s) 774
BstXI CCANNNNNNTGG 1 cut(s) 799
BsuRI GGCC 1 cut(s) 791
BtgI CCRYGG 2 cut(s) 97, 858
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 215
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 3 cut(s) 305, 605, 716
Cac8I GCNNGC 4 cut(s) 136, 432, 449, 534
CaiI CAGNNNCTG 2 cut(s) 650, 686
CfoI GCGC 3 cut(s) 350, 392, 598
CseI GACGC 1 cut(s) 628
Csp6I GTAC 5 cut(s) 154, 295, 469, 491, 724
CviAII CATG 4 cut(s) 98, 431, 494, 859
CviQI GTAC 5 cut(s) 154, 295, 469, 491, 724
DdeI CTNAG 5 cut(s) 43, 202, 435, 708, 751
DinI GGCGCC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 5 cut(s) 28, 64, 514, 637, 659
EarI CTCTTC 5 cut(s) 28, 64, 514, 637, 659
Eco130I CCWWGG 2 cut(s) 97, 858
Eco32I GATATC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 683
EcoRV GATATC 1 cut(s) 748
EcoT14I CCWWGG 2 cut(s) 97, 858
EcoT22I ATGCAT 1 cut(s) 341
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 2 cut(s) 97, 858
FaeI CATG 4 cut(s) 101, 434, 497, 862
FaiI YATR 9 cut(s) 99, 132, 288, 387, 417, 432, 495, 717, 860
FaqI GGGAC 2 cut(s) 360, 562
FatI CATG 4 cut(s) 97, 430, 493, 858
Fnu4HI GCNGC 3 cut(s) 139, 684, 687
FokI GGATG 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 139, 684, 687
FspBI CTAG 5 cut(s) 368, 533, 552, 720, 818
FspI TGCGCA 1 cut(s) 597
GlaI GCGC 3 cut(s) 349, 391, 597
GluI GCNGC 3 cut(s) 139, 684, 687
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 1 cut(s) 791
HapII CCGG 1 cut(s) 850
HgaI GACGC 1 cut(s) 628
HhaI GCGC 3 cut(s) 350, 392, 598
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 4 cut(s) 101, 434, 497, 862
Hin6I GCGC 3 cut(s) 348, 390, 596
HinP1I GCGC 3 cut(s) 348, 390, 596
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 3 cut(s) 54, 355, 755
HpaII CCGG 1 cut(s) 850
HphI GGTGA 4 cut(s) 159, 261, 320, 364
Hpy188I TCNGA 3 cut(s) 251, 516, 754
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 2 cut(s) 20, 269
HpyCH4III ACNGT 3 cut(s) 329, 490, 787
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 5 cut(s) 134, 303, 339, 400, 544
HpyF10VI GCNNNNNNNGC 3 cut(s) 345, 636, 818
HpyF3I CTNAG 5 cut(s) 43, 202, 435, 708, 751
HpySE526I ACGT 1 cut(s) 528
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 4 cut(s) 101, 434, 497, 862
HspAI GCGC 3 cut(s) 348, 390, 596
KasI GGCGCC 1 cut(s) 389
LguI GCTCTTC 1 cut(s) 637
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 8 cut(s) 62, 120, 285, 311, 561, 712, 720, 863
Lsp1109I GCAGC 3 cut(s) 125, 673, 695
LweI GCATC 2 cut(s) 348, 637
MaeI CTAG 5 cut(s) 368, 533, 552, 720, 818
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 3 cut(s) 308, 361, 402
MlsI TGGCCA 1 cut(s) 791
MluNI TGGCCA 1 cut(s) 791
Mly113I GGCGCC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 229
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 58
MscI TGGCCA 1 cut(s) 791
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 2 cut(s) 498, 797
Msp20I TGGCCA 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 1 cut(s) 850
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 3 cut(s) 345, 636, 818
NarI GGCGCC 1 cut(s) 390
NcoI CCATGG 2 cut(s) 97, 858
NheI GCTAGC 1 cut(s) 532
NlaIII CATG 4 cut(s) 101, 434, 497, 862
NlaIV GGNNCC 4 cut(s) 103, 259, 280, 391
NmuCI GTSAC 2 cut(s) 308, 361
NsbI TGCGCA 1 cut(s) 597
NsiI ATGCAT 1 cut(s) 341
NspI RCATGY 1 cut(s) 434
NspV TTCGAA 1 cut(s) 332
PaeI GCATGC 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 54, 355, 755
PkrI GCNGC 3 cut(s) 140, 685, 688
PluTI GGCGCC 1 cut(s) 393
PspN4I GGNNCC 4 cut(s) 103, 259, 280, 391
PstNI CAGNNNCTG 2 cut(s) 650, 686
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 5 cut(s) 155, 296, 470, 492, 725
RsaNI GTAC 5 cut(s) 154, 295, 469, 491, 724
RseI CAYNNNNRTG 2 cut(s) 498, 797
SapI GCTCTTC 1 cut(s) 637
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 3 cut(s) 139, 684, 687
SfaNI GCATC 2 cut(s) 348, 637
SfoI GGCGCC 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 498, 797
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
SpeI ACTAGT 1 cut(s) 551
SphI GCATGC 1 cut(s) 434
SsiI CCGC 2 cut(s) 350, 689
SspDI GGCGCC 1 cut(s) 389
SspMI CTAG 5 cut(s) 368, 533, 552, 720, 818
StyI CCWWGG 2 cut(s) 97, 858
TaaI ACNGT 3 cut(s) 329, 490, 787
TaiI ACGT 1 cut(s) 531
TaqI TCGA 2 cut(s) 332, 342
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 3 cut(s) 54, 355, 755
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 3 cut(s) 305, 605, 716
TseFI GTSAC 2 cut(s) 308, 361
TseI GCWGC 3 cut(s) 138, 683, 686
Tsp45I GTSAC 2 cut(s) 308, 361
TspDTI ATGAA 2 cut(s) 115, 510
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 3 cut(s) 305, 605, 716
XceI RCATGY 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 5 cut(s) 368, 533, 552, 720, 818
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.