RchiOBHm_Chr1g0360191

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008212
Physical Location & Seq
Forward (+)
51949124 .. 51953357
4234 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2004 bp
ATGTCGAAACCGCTCGGAGAATACCTCAAGGAAGAAAAGGAAGCAGAGGAGGCGGCCGCTCGGCCTCCGCCTTCTGCTCATGCGACGTCGCCGGAGATAGACTCGCCGGGATCGGATCCATCGATGAAACTTTCGGATTTCACTGCTGATGACGACTCGGTGTCTTTCGGGCTTCAGCGGCTCAAGGAGATTGCTGAAGCAGCCGAGAAGAAGAAGAAGGCGGAGAAGAATAAGAAGAAGAAGGAGAAGAAGAAGATGAAGAAGCTGCAGGCGGAGAAAGATGTCGGCTCTTCCAAGACTGAAGGTGCGACTCCGAGTGATGAGGATGACTCGACGAAATCGGAAGAAGGTTCTGGAACCTGTGAGAAGAGCGAGAGTGCCGAATTGGCCAAGAAAGATCCATTGTCGCCGGAGAAGTGTACGGGAACGTCCGCGACGCCGCCGTCTACAGGGAAGGCAAAGAAGTCGGTGAAACTGAAACTGCAGAGGCCCGACGAAGCTCCGAAGCAGACGACGTCGCTGCTGATGTCGTATCAAAGTATGAGGAGTCCTGAGTTGAGTGCACTCATCGTGGAATCTTCGAAGGAATCTTCGGCGGCTATGGTGGAGGAGGCCTGGGAGAGACTCAAGAAGTCTTACGTTTACTACAGAGGCAAGCCGGTGGGGACTCTTGCTGCTATGGATCCAACGGCCGAGGCTCTAAACTACAATCAGGTATTTGTGAGAGATTTTGTTCCTACTGGTTTGGCCTGCCTCATGAAGAATCCAGCAGAGCCAGAGATAGTAAAGAACTTTCTCCTAAAGACCCTCCACCTCCAAGGTCGAGAGAAAATGGTTGATAACTTTACACTTGGAGAAGGTGTTCTTCCTGCGAGCTTTAAGGTTTCAAATGATTCAGATCGTGCCAAGGAAACTATAGTTGCAGATTTTGGTGGTAGCGCAATTGGAAGAGTTGCTCCGGTTGATTCTGGATTCTGGTGGATCATATTGCTGTGGTCATATAACAGATGCACTCGTGATCATGCGCTGGTGGAGCTCCCTGCGGTGCAAAATGGAATGAAACTGATACTCAACCTCTGCCTTTCAGATGGCTTTGACACATTTCCAACACTTCTTTGTGCAGATGGCTGCAGTATGATTGACAGGAGGATGGGCATATATGGTTATCCAATTGAGATCCAAGCACTTTTCTACTTTGCATTGAGATGTGCAATGCAGCTGTTGAAGCCGGAGCGTGGTGGTAAGGAATTTCTTGAGAGGATAGATAAGCGCATTATAGCTCTCAGCTTTCACATCCAGAAGTACTACTGGCTTGACTTTACTCATCTCAATAACATATACCGCTACAAAACGGAGGAGTATTCCCATACAGCTGTCAACAAGTTCAATGTGATGCCAGAGTCCATCCCTGATTGGGTTTTTGATTTCATGCCATTGCGAGGAGGGTATTTGATTGGCAATGTAAGCCCAGCTCGCATGGATTTCCGGTGGTTCTTAGTTGGGAACTGCATTGCAATTCTCAGCTGTTTAGCCACACCTGATCAAGCTATAGCCATAATGGATCTGATTGAGGAGCGTTGGGAGGATTTGATCGGTGAGATGCCTCTGAAAATTGCATATCCAGCATTGGAGGGACATGAGTGGAGGACAATCACAGGATATGACCCCAAAAATACACGATGGAGCTACCACAATGGCGGTTCCTGGCCAACACTGCTGTGGCTGCTGGCTGCGGCAAGCATCAAGACTGGGAGGCCTCAGATTGCAAAGAGGGCAATAGAGCTGGCAGAGCAGCGGCTCTCCAAAGACGGGTGGCCTGAGTACTATGATGGTAAGACAGGGAGATACATTGGGAAGCAAGCGAGGAGGTACCAGACATGGAGCATCTCTGGCTATTTGATTGCTAAGAAGATGATAGAGAATCCATCCAATCTTCTATTGGTTTCTCTCGAGGAGGACAAGAAAATTGCCAAGCCAAGGCTCGCCCGTTCTGCTACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

667

Amino Acids

74.84

Weight (kDa)

8.27

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 205 - 642 1.9e-211 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AatII GACGTC 2 cut(s) 89, 518
Acc65I GGTACC 1 cut(s) 1869
AccB1I GGYRCC 1 cut(s) 1869
AccBSI CCGCTC 2 cut(s) 13, 59
AccI GTMKAC 1 cut(s) 446
AccII CGCG 1 cut(s) 434
AclWI GGATC 9 cut(s) 110, 118, 123, 392, 677, 690, 989, 1171, 1569
AcoI YGGCCR 4 cut(s) 54, 387, 690, 1706
AcsI RAATTY 1 cut(s) 1247
AcuI CTGAAG 3 cut(s) 158, 216, 321
AcyI GRCGYC 3 cut(s) 86, 437, 515
AfaI GTAC 4 cut(s) 421, 1304, 1823, 1871
AfiI CCNNNNNNNGG 6 cut(s) 449, 1235, 1414, 1439, 1809, 1977
AgsI TTSAA 3 cut(s) 888, 1225, 1387
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 614, 1703
AleI CACNNNNGTG 1 cut(s) 1717
AloI GAACNNNNNNTCC 2 cut(s) 846, 878
Alw21I GWGCWC 2 cut(s) 565, 1038
Alw26I GTCTC 1 cut(s) 616
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 9 cut(s) 110, 118, 123, 392, 677, 690, 989, 1171, 1569
Ama87I CYCGRG 1 cut(s) 1949
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 9 cut(s) 200, 265, 520, 674, 1128, 1216, 1723, 1730, 1792
ApoI RAATTY 1 cut(s) 1247
Asp700I GAANNNNTTC 1 cut(s) 861
Asp718I GGTACC 1 cut(s) 1869
AspLEI GCGC 3 cut(s) 941, 1027, 1272
AspS9I GGNCC 1 cut(s) 489
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 481, 1607
AsuII TTCGAA 1 cut(s) 581
AvaI CYCGRG 1 cut(s) 1949
BaeGI GKGCMC 1 cut(s) 565
BalI TGGCCA 2 cut(s) 389, 1708
BamHI GGATCC 2 cut(s) 115, 682
BanI GGYRCC 1 cut(s) 1869
BanII GRGCYC 1 cut(s) 1038
BauI CACGAG 1 cut(s) 1014
Bbv12I GWGCWC 2 cut(s) 565, 1038
BbvI GCAGC 9 cut(s) 212, 252, 507, 661, 1115, 1228, 1710, 1717, 1804
BccI CCATC 8 cut(s) 127, 1082, 1118, 1144, 1412, 1674, 1823, 1933
BceAI ACGGC 2 cut(s) 427, 705
BcgI CGANNNNNNTGC 4 cut(s) 447, 481, 502, 536
BciT130I CCWGG 2 cut(s) 616, 1705
BclI TGATCA 2 cut(s) 1018, 1540
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 616
BfmI CTRYAG 7 cut(s) 266, 447, 482, 646, 915, 1129, 1548
BglI GCCNNNNNGGC 1 cut(s) 386
BmcAI AGTACT 2 cut(s) 1304, 1823
Bme1390I CCNGG 3 cut(s) 108, 616, 1705
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 1949
BmgT120I GGNCC 1 cut(s) 489
BmiI GGNNCC 5 cut(s) 117, 358, 684, 1702, 1871
BmrFI CCNGG 3 cut(s) 108, 616, 1705
BmrI ACTGGG 1 cut(s) 1758
BmsI GCATC 5 cut(s) 998, 1383, 1590, 1749, 1893
BmuI ACTGGG 1 cut(s) 1758
BplI GAGNNNNNCTC 6 cut(s) 9, 41, 86, 118, 314, 346
Bpu14I TTCGAA 1 cut(s) 581
BpuEI CTTGAG 4 cut(s) 11, 167, 611, 1274
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 3 cut(s) 86, 437, 515
BsaJI CCNNGG 5 cut(s) 615, 693, 817, 906, 1976
BsaWI WCCGGW 2 cut(s) 958, 1485
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 6 cut(s) 449, 1235, 1414, 1439, 1809, 1977
Bse118I RCCGGY 1 cut(s) 658
Bse1I ACTGG 3 cut(s) 745, 1313, 1753
Bse3DI GCAATG 4 cut(s) 1218, 1433, 1465, 1509
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 2 cut(s) 616, 1705
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 5 cut(s) 615, 693, 817, 906, 1976
BseGI GGATG 5 cut(s) 331, 1155, 1293, 1404, 1925
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 6 cut(s) 449, 1235, 1414, 1439, 1809, 1977
BseMI GCAATG 4 cut(s) 1218, 1433, 1465, 1509
BseMII CTCAG 5 cut(s) 543, 1297, 1534, 1772, 1809
BseNI ACTGG 3 cut(s) 745, 1313, 1753
BseRI GAGGAG 8 cut(s) 62, 559, 623, 1370, 1455, 1586, 1879, 1967
BseSI GKGCMC 1 cut(s) 565
BseX3I CGGCCG 2 cut(s) 54, 690
BseXI GCAGC 9 cut(s) 212, 252, 507, 661, 1115, 1228, 1710, 1717, 1804
BseYI CCCAGC 1 cut(s) 1468
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 2 cut(s) 57, 693
BshNI GGYRCC 1 cut(s) 1869
BshVI ATCGAT 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 57, 693
BsiHKAI GWGCWC 2 cut(s) 565, 1038
BsiHKCI CYCGRG 1 cut(s) 1949
BsiSI CCGG 7 cut(s) 92, 107, 410, 659, 959, 1229, 1486
BslFI GGGAC 2 cut(s) 679, 1647
BslI CCNNNNNNNGG 6 cut(s) 449, 1235, 1414, 1439, 1809, 1977
BsmAI GTCTC 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 679, 1647
BsoBI CYCGRG 1 cut(s) 1949
Bsp119I TTCGAA 1 cut(s) 581
Bsp1286I GDGCHC 2 cut(s) 565, 1038
BspCNI CTCAG 5 cut(s) 544, 1296, 1533, 1771, 1810
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 434
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 5 cut(s) 117, 358, 684, 1702, 1871
BspMAI CTGCAG 3 cut(s) 270, 486, 1133
BspPI GGATC 9 cut(s) 110, 118, 123, 392, 677, 690, 989, 1171, 1569
BspQI GCTCTTC 2 cut(s) 295, 362
BspT104I TTCGAA 1 cut(s) 581
BspT107I GGYRCC 1 cut(s) 1869
BsrBI CCGCTC 2 cut(s) 13, 59
BsrDI GCAATG 4 cut(s) 1218, 1433, 1465, 1509
BsrFI RCCGGY 1 cut(s) 658
BsrI ACTGG 3 cut(s) 745, 1313, 1753
BssAI RCCGGY 1 cut(s) 658
BssECI CCNNGG 5 cut(s) 615, 693, 817, 906, 1976
BssNI GRCGYC 3 cut(s) 86, 437, 515
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 3 cut(s) 817, 906, 1976
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 2 cut(s) 616, 1705
Bst4CI ACNGT 1 cut(s) 1999
Bst6I CTCTTC 3 cut(s) 295, 362, 943
BstACI GRCGYC 3 cut(s) 86, 437, 515
BstBI TTCGAA 1 cut(s) 581
BstDEI CTNAG 7 cut(s) 552, 1283, 1495, 1520, 1758, 1818, 1905
BstF5I GGATG 5 cut(s) 331, 1155, 1293, 1404, 1925
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 3 cut(s) 941, 1027, 1272
BstMAI GTCTC 1 cut(s) 616
BstMCI CGRYCG 2 cut(s) 57, 693
BstNI CCWGG 2 cut(s) 616, 1705
BstSCI CCNGG 3 cut(s) 106, 614, 1703
BstSFI CTRYAG 7 cut(s) 266, 447, 482, 646, 915, 1129, 1548
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 9 cut(s) 212, 252, 507, 661, 1115, 1228, 1710, 1717, 1804
BstX2I RGATCY 5 cut(s) 115, 397, 682, 1176, 1561
BstYI RGATCY 5 cut(s) 115, 397, 682, 1176, 1561
BstZI CGGCCG 2 cut(s) 54, 690
Bsu15I ATCGAT 1 cut(s) 122
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 5 cut(s) 331, 1155, 1293, 1404, 1925
BtsI GCAGTG 2 cut(s) 141, 1712
BtsIMutI CAGTG 2 cut(s) 141, 1712
CciI TCATGA 1 cut(s) 756
CciNI GCGGCCGC 1 cut(s) 54
CfoI GCGC 3 cut(s) 941, 1027, 1272
Cfr10I RCCGGY 1 cut(s) 658
Cfr13I GGNCC 1 cut(s) 489
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 1 cut(s) 445
Csp6I GTAC 4 cut(s) 420, 1303, 1822, 1870
CviAII CATG 7 cut(s) 80, 757, 1022, 1429, 1477, 1637, 1878
CviQI GTAC 4 cut(s) 420, 1303, 1822, 1870
DdeI CTNAG 7 cut(s) 552, 1283, 1495, 1520, 1758, 1818, 1905
DrdI GACNNNNNNGTC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 4 cut(s) 54, 387, 690, 1706
EagI CGGCCG 2 cut(s) 54, 690
Eam1104I CTCTTC 3 cut(s) 295, 362, 943
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 3 cut(s) 295, 362, 943
EciI GGCGGA 3 cut(s) 57, 236, 287
Ecl136II GAGCTC 1 cut(s) 1036
EclXI CGGCCG 2 cut(s) 54, 690
Eco130I CCWWGG 3 cut(s) 817, 906, 1976
Eco147I AGGCCT 2 cut(s) 614, 1756
Eco24I GRGCYC 1 cut(s) 1038
Eco52I CGGCCG 2 cut(s) 54, 690
Eco53kI GAGCTC 1 cut(s) 1036
Eco57I CTGAAG 3 cut(s) 158, 216, 321
Eco88I CYCGRG 1 cut(s) 1949
EcoICRI GAGCTC 1 cut(s) 1036
EcoRII CCWGG 2 cut(s) 614, 1703
EcoT14I CCWWGG 3 cut(s) 817, 906, 1976
EcoT38I GRGCYC 1 cut(s) 1038
ErhI CCWWGG 3 cut(s) 817, 906, 1976
FaeI CATG 7 cut(s) 83, 760, 1025, 1432, 1480, 1640, 1881
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 2 cut(s) 679, 1647
FatI CATG 7 cut(s) 79, 756, 1021, 1428, 1476, 1636, 1877
FbaI TGATCA 2 cut(s) 1018, 1540
FblI GTMKAC 1 cut(s) 446
FokI GGATG 5 cut(s) 338, 1162, 1280, 1391, 1912
FriOI GRGCYC 1 cut(s) 1038
GlaI GCGC 3 cut(s) 940, 1026, 1271
GsaI CCCAGC 1 cut(s) 1472
HapII CCGG 7 cut(s) 92, 107, 410, 659, 959, 1229, 1486
HgaI GACGC 1 cut(s) 445
HhaI GCGC 3 cut(s) 941, 1027, 1272
Hin1I GRCGYC 3 cut(s) 86, 437, 515
Hin1II CATG 7 cut(s) 83, 760, 1025, 1432, 1480, 1640, 1881
Hin6I GCGC 3 cut(s) 939, 1025, 1270
HinP1I GCGC 3 cut(s) 939, 1025, 1270
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HpaII CCGG 7 cut(s) 92, 107, 410, 659, 959, 1229, 1486
HphI GGTGA 2 cut(s) 481, 1607
Hpy166II GTNNAC 5 cut(s) 420, 447, 563, 643, 1378
Hpy8I GTNNAC 5 cut(s) 420, 447, 563, 643, 1378
Hpy99I CGWCG 7 cut(s) 88, 91, 337, 439, 497, 517, 520
HpyAV CCTTC 8 cut(s) 81, 211, 235, 296, 341, 448, 577, 851
HpyCH4III ACNGT 1 cut(s) 1999
HpyCH4IV ACGT 4 cut(s) 86, 428, 515, 639
HpyF3I CTNAG 7 cut(s) 552, 1283, 1495, 1520, 1758, 1818, 1905
HpySE526I ACGT 4 cut(s) 86, 428, 515, 639
Hsp92I GRCGYC 3 cut(s) 86, 437, 515
Hsp92II CATG 7 cut(s) 83, 760, 1025, 1432, 1480, 1640, 1881
HspAI GCGC 3 cut(s) 939, 1025, 1270
KpnI GGTACC 1 cut(s) 1873
Ksp22I TGATCA 2 cut(s) 1018, 1540
LguI GCTCTTC 2 cut(s) 295, 362
LmnI GCTCC 8 cut(s) 505, 961, 1033, 1041, 1231, 1573, 1683, 1881
Lsp1109I GCAGC 9 cut(s) 212, 252, 507, 661, 1115, 1228, 1710, 1717, 1804
LweI GCATC 5 cut(s) 998, 1383, 1590, 1749, 1893
MaeII ACGT 4 cut(s) 86, 428, 515, 639
MbiI CCGCTC 2 cut(s) 13, 59
MfeI CAATTG 2 cut(s) 942, 1170
MflI RGATCY 5 cut(s) 115, 397, 682, 1176, 1561
MhlI GDGCHC 2 cut(s) 565, 1038
MlsI TGGCCA 2 cut(s) 389, 1708
MluCI AATT 7 cut(s) 383, 942, 1170, 1247, 1515, 1611, 1965
MluNI TGGCCA 2 cut(s) 389, 1708
MlyI GAGTC 8 cut(s) 95, 149, 304, 323, 556, 618, 661, 1409
MmeI TCCRAC 2 cut(s) 710, 1130
Mox20I TGGCCA 2 cut(s) 389, 1708
MroXI GAANNNNTTC 1 cut(s) 861
MscI TGGCCA 2 cut(s) 389, 1708
MseI TTAA 2 cut(s) 879, 2002
MslI CAYNNNNRTG 2 cut(s) 1204, 1717
Msp20I TGGCCA 2 cut(s) 389, 1708
MspA1I CMGCKG 5 cut(s) 178, 1219, 1373, 1524, 1795
MspI CCGG 7 cut(s) 92, 107, 410, 659, 959, 1229, 1486
MspR9I CCNGG 3 cut(s) 108, 616, 1705
MunI CAATTG 2 cut(s) 942, 1170
MvaI CCWGG 2 cut(s) 616, 1705
MvnI CGCG 1 cut(s) 434
NciI CCSGG 1 cut(s) 108
NlaIII CATG 7 cut(s) 83, 760, 1025, 1432, 1480, 1640, 1881
NlaIV GGNNCC 5 cut(s) 117, 358, 684, 1702, 1871
NmeAIII GCCGAG 3 cut(s) 40, 229, 718
NotI GCGGCCGC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 581
OliI CACNNNNGTG 1 cut(s) 1717
PaeR7I CTCGAG 1 cut(s) 1949
PagI TCATGA 1 cut(s) 756
PceI AGGCCT 2 cut(s) 614, 1756
PciSI GCTCTTC 2 cut(s) 295, 362
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 7 cut(s) 575, 587, 763, 893, 965, 972, 1921
PflFI GACNNNGTC 1 cut(s) 514
PleI GAGTC 8 cut(s) 95, 149, 304, 323, 555, 618, 661, 1408
PpsI GAGTC 8 cut(s) 95, 149, 304, 323, 555, 618, 661, 1408
Psp124BI GAGCTC 1 cut(s) 1038
Psp6I CCWGG 2 cut(s) 614, 1703
PspFI CCCAGC 1 cut(s) 1468
PspGI CCWGG 2 cut(s) 614, 1703
PspN4I GGNNCC 5 cut(s) 117, 358, 684, 1702, 1871
PspPI GGNCC 1 cut(s) 489
PstI CTGCAG 3 cut(s) 270, 486, 1133
PsuI RGATCY 5 cut(s) 115, 397, 682, 1176, 1561
PsyI GACNNNGTC 1 cut(s) 514
PvuII CAGCTG 3 cut(s) 1219, 1373, 1524
RsaI GTAC 4 cut(s) 421, 1304, 1823, 1871
RsaNI GTAC 4 cut(s) 420, 1303, 1822, 1870
RseI CAYNNNNRTG 2 cut(s) 1204, 1717
SacI GAGCTC 1 cut(s) 1038
SapI GCTCTTC 2 cut(s) 295, 362
SaqAI TTAA 2 cut(s) 879, 2002
Sau96I GGNCC 1 cut(s) 489
ScaI AGTACT 2 cut(s) 1304, 1823
SchI GAGTC 8 cut(s) 95, 149, 304, 323, 556, 618, 661, 1409
ScrFI CCNGG 3 cut(s) 108, 616, 1705
SduI GDGCHC 2 cut(s) 565, 1038
SfaNI GCATC 5 cut(s) 998, 1383, 1590, 1749, 1893
SfcI CTRYAG 7 cut(s) 266, 447, 482, 646, 915, 1129, 1548
Sfr274I CTCGAG 1 cut(s) 1949
SfuI TTCGAA 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 1949
SmiMI CAYNNNNRTG 2 cut(s) 1204, 1717
SmlI CTYRAG 5 cut(s) 26, 182, 626, 1253, 1949
SmoI CTYRAG 5 cut(s) 26, 182, 626, 1253, 1949
Sse9I AATT 7 cut(s) 383, 942, 1170, 1247, 1515, 1611, 1965
SseBI AGGCCT 2 cut(s) 614, 1756
SstI GAGCTC 1 cut(s) 1038
StuI AGGCCT 2 cut(s) 614, 1756
StyD4I CCNGG 3 cut(s) 106, 614, 1703
StyI CCWWGG 3 cut(s) 817, 906, 1976
TaaI ACNGT 1 cut(s) 1999
TaiI ACGT 4 cut(s) 89, 431, 518, 642
TaqI TCGA 6 cut(s) 5, 122, 332, 581, 823, 1950
TasI AATT 7 cut(s) 383, 942, 1170, 1247, 1515, 1611, 1965
TatI WGTACW 2 cut(s) 1302, 1821
TauI GCSGC 7 cut(s) 56, 59, 181, 442, 599, 1736, 1798
TfiI GAWTC 7 cut(s) 575, 587, 763, 893, 965, 972, 1921
Tru1I TTAA 2 cut(s) 879, 2002
Tru9I TTAA 2 cut(s) 879, 2002
TscAI CASTG 2 cut(s) 148, 1719
TseI GCWGC 9 cut(s) 200, 265, 520, 674, 1128, 1216, 1723, 1730, 1792
TspDTI ATGAA 5 cut(s) 140, 272, 773, 1073, 1417
TspGWI ACGGA 1 cut(s) 1367
TspRI CASTG 2 cut(s) 148, 1719
Tth111I GACNNNGTC 1 cut(s) 514
VneI GTGCAC 1 cut(s) 561
XapI RAATTY 1 cut(s) 1247
XcmI CCANNNNNNNNNTGG 2 cut(s) 1716, 1936
XhoI CTCGAG 1 cut(s) 1949
XmiI GTMKAC 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 861
ZraI GACGTC 2 cut(s) 87, 516
ZrmI AGTACT 2 cut(s) 1304, 1823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.