RchiOBHm_Chr1g0367701

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010937
Physical Location & Seq
Forward (+)
58000096 .. 58004094
3999 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGAACCTCTCCACCACTCCCACTCCACGCGCCACCCCGGCCGACTCGCGCGTCCTGCTCGGCTTGTCCGAACCGGAACTCCAGCAGCTCGCCCTTGACTTCAGTCAGCAAGGCTACAGAGGCAAGCAGCTTCATCATCTCATTTACAAAAGAAAGATTAAAGACATTCAGGATTTCAGTCAAGTGCCTCTGGCATTCAGGAATGAGCTTGAGGAAGCTGGATGGAAGGTTGGGAGGTCACCTATTTACAATACTGTTACTGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACCGATTGATTGAAACTGTTGGCATACCGGTTCAAGACGAGAAGGGTTTGATGCGCCTTACAGCATGTGTCTCATCACAGGTGGGATGCCCTCTGCGTTGCTCTTTTTGCGCAACAGGAAAAGGAGGGTTTTCAAGGAACCTTAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAGATATTCCAGCATAGGGTGACTAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTCAAGTCTGTACTTGAAGCACACCAGTGCTTGAATAAGGATGTGCAAATTGGGCAAAGAATGATGACAATTTCTACTGTGGGGGTTCCAAATACAATTAAAAGGCTGGCATCTCACAAGCTTCAGTCAACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAACTTAGGGAAACAATTGTACCAAGTGCGAAAGCATATCCTCTAAGTGCACTTATGAAGGATTGCAGCGACTATTTCAATGAAACAAATAGGCGCGTTTCCTTTGAGTATGCGCTTTTAGCTGGAGTCAATGATGCAGTAGAGCACGCACGTGAACTTGCAGAGCTACTTCATCAGTGGGGACGAGGTTATCATGTGAACTTGATACCTTTTAATCCAATAGAAGGTTCTGAGTATAAGCGTCCATCCAAGAGAGCTGTCCAGGCATTTGCTGCTGCTCTGGAGTCCGGTAAAATAACTGTTAGCACACGTCAAACAAGGGGCCTTGATGCAGATGCAGCTTGTGGCCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGGTCTCCGAGTCTGACAACTTGCAACCTGAACCTGAAGCAGATGTTGCAGTTGCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

383

Amino Acids

42.48

Weight (kDa)

8.69

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 126 - 289 3.3e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
Acc16I TGCGCA 1 cut(s) 415
Acc36I ACCTGC 1 cut(s) 460
AccII CGCG 4 cut(s) 30, 49, 51, 807
AcoI YGGCCR 2 cut(s) 39, 1057
AcuI CTGAAG 3 cut(s) 85, 656, 1146
AcvI CACGTG 1 cut(s) 863
AfaI GTAC 2 cut(s) 561, 732
AfiI CCNNNNNNNGG 2 cut(s) 502, 935
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 1019
AgeI ACCGGT 1 cut(s) 331
AgsI TTSAA 6 cut(s) 317, 338, 438, 566, 583, 790
AjiI CACGTC 1 cut(s) 1022
AjnI CCWGG 1 cut(s) 972
AjuI GAANNNNNNNTTGG 4 cut(s) 922, 954, 1073, 1105
AleI CACNNNNGTG 2 cut(s) 574, 861
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
Alw21I GWGCWC 2 cut(s) 763, 858
Alw26I GTCTC 2 cut(s) 379, 1099
Alw44I GTGCAC 1 cut(s) 759
AoxI GGCC 3 cut(s) 39, 1033, 1057
ApaLI GTGCAC 1 cut(s) 759
ApeKI GCWGC 7 cut(s) 85, 127, 263, 777, 983, 986, 1049
AsiGI ACCGGT 1 cut(s) 331
AspLEI GCGC 6 cut(s) 32, 51, 360, 416, 807, 826
AspS9I GGNCC 1 cut(s) 1033
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 3 cut(s) 231, 517, 545
BaeGI GKGCMC 1 cut(s) 763
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BalI TGGCCA 1 cut(s) 1059
BbrPI CACGTG 1 cut(s) 863
Bbv12I GWGCWC 2 cut(s) 763, 858
BbvI GCAGC 7 cut(s) 97, 139, 250, 789, 970, 973, 1061
BccI CCATC 3 cut(s) 216, 263, 964
BciT130I CCWGG 1 cut(s) 974
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 379, 1099
BfmI CTRYAG 1 cut(s) 115
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 460
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 7 cut(s) 86, 128, 264, 778, 984, 987, 1050
BlsI GCNGC 7 cut(s) 87, 129, 265, 779, 985, 988, 1051
Bme1390I CCNGG 2 cut(s) 38, 974
BmgBI CACGTC 1 cut(s) 1022
BmgT120I GGNCC 1 cut(s) 1033
BmiI GGNNCC 3 cut(s) 443, 636, 1034
BmrFI CCNGG 2 cut(s) 38, 974
BmsI GCATC 6 cut(s) 345, 380, 668, 835, 1030, 1036
BoxI GACNNNNGTC 1 cut(s) 102
BpmI CTGGAG 3 cut(s) 65, 855, 1013
BpuEI CTTGAG 2 cut(s) 230, 536
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 863
BsaI GGTCTC 1 cut(s) 1099
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 3 cut(s) 73, 331, 998
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 63, 93
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 935
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 574
BseBI CCWGG 1 cut(s) 974
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 4 cut(s) 227, 395, 595, 956
BseLI CCNNNNNNNGG 2 cut(s) 502, 935
BseMII CTCAG 1 cut(s) 933
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 500
BseSI GKGCMC 1 cut(s) 763
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 7 cut(s) 97, 139, 250, 789, 970, 973, 1061
Bsh1236I CGCG 4 cut(s) 30, 49, 51, 807
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 41, 1035, 1059
BshTI ACCGGT 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 763, 858
BsiSI CCGG 4 cut(s) 38, 74, 332, 999
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 2 cut(s) 502, 935
BsmAI GTCTC 2 cut(s) 379, 1099
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 3 cut(s) 41, 1035, 1059
Bso31I GGTCTC 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 763, 858
BspANI GGCC 3 cut(s) 41, 1035, 1059
BspCNI CTCAG 1 cut(s) 934
BspFNI CGCG 4 cut(s) 30, 49, 51, 807
BspLI GGNNCC 3 cut(s) 443, 636, 1034
BspMI ACCTGC 1 cut(s) 460
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 1099
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 974
Bst4CI ACNGT 5 cut(s) 256, 277, 322, 628, 1012
Bst6I CTCTTC 1 cut(s) 1076
BstAFI CTTAAG 1 cut(s) 446
BstAPI GCANNNNNTGC 2 cut(s) 983, 1136
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 7 cut(s) 90, 125, 657, 695, 699, 858, 1061
BstDEI CTNAG 4 cut(s) 716, 755, 942, 1064
BstEII GGTNACC 1 cut(s) 237
BstF5I GGATG 4 cut(s) 227, 395, 595, 956
BstFNI CGCG 4 cut(s) 30, 49, 51, 807
BstHHI GCGC 6 cut(s) 32, 51, 360, 416, 807, 826
BstMAI GTCTC 2 cut(s) 379, 1099
BstMCI CGRYCG 1 cut(s) 42
BstNI CCWGG 1 cut(s) 974
BstNSI RCATGY 3 cut(s) 372, 701, 1149
BstPAI GACNNNNGTC 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 237
BstSCI CCNGG 2 cut(s) 36, 972
BstSFI CTRYAG 1 cut(s) 115
BstSLI GKGCMC 1 cut(s) 763
BstUI CGCG 4 cut(s) 30, 49, 51, 807
BstV1I GCAGC 7 cut(s) 97, 139, 250, 789, 970, 973, 1061
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 3 cut(s) 41, 1035, 1059
BtrI CACGTC 1 cut(s) 1022
BtsCI GGATG 4 cut(s) 227, 395, 595, 956
BtsIMutI CAGTG 3 cut(s) 273, 581, 893
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 7 cut(s) 90, 125, 657, 695, 699, 858, 1061
CfoI GCGC 6 cut(s) 32, 51, 360, 416, 807, 826
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 1033
CseI GACGC 2 cut(s) 40, 941
Csp6I GTAC 2 cut(s) 560, 731
CspAI ACCGGT 1 cut(s) 331
CviAII CATG 6 cut(s) 369, 455, 523, 698, 905, 1146
CviQI GTAC 2 cut(s) 560, 731
DdeI CTNAG 4 cut(s) 716, 755, 942, 1064
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 2 cut(s) 39, 1057
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 1076
EarI CTCTTC 1 cut(s) 1076
EclXI CGGCCG 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 1099
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 3 cut(s) 85, 656, 1146
Eco72I CACGTG 1 cut(s) 863
Eco91I GGTNACC 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 1033
EcoO65I GGTNACC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 972
FaeI CATG 6 cut(s) 372, 458, 526, 701, 908, 1149
FaqI GGGAC 1 cut(s) 906
FatI CATG 6 cut(s) 368, 454, 522, 697, 904, 1145
Fnu4HI GCNGC 7 cut(s) 86, 128, 264, 778, 984, 987, 1050
FokI GGATG 4 cut(s) 234, 402, 602, 943
Fsp4HI GCNGC 7 cut(s) 86, 128, 264, 778, 984, 987, 1050
FspI TGCGCA 1 cut(s) 415
GlaI GCGC 6 cut(s) 31, 50, 359, 415, 806, 825
GluI GCNGC 7 cut(s) 86, 128, 264, 778, 984, 987, 1050
GsuI CTGGAG 3 cut(s) 65, 855, 1013
HaeIII GGCC 3 cut(s) 41, 1035, 1059
HapII CCGG 4 cut(s) 38, 74, 332, 999
HgaI GACGC 2 cut(s) 40, 941
HhaI GCGC 6 cut(s) 32, 51, 360, 416, 807, 826
Hin1II CATG 6 cut(s) 372, 458, 526, 701, 908, 1149
Hin6I GCGC 6 cut(s) 30, 49, 358, 414, 805, 824
HinP1I GCGC 6 cut(s) 30, 49, 358, 414, 805, 824
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 44, 837, 995, 1084, 1100
HpaII CCGG 4 cut(s) 38, 74, 332, 999
HphI GGTGA 3 cut(s) 231, 517, 545
Hpy166II GTNNAC 5 cut(s) 536, 678, 761, 866, 910
Hpy188I TCNGA 4 cut(s) 70, 943, 1099, 1105
Hpy188III TCNNGA 5 cut(s) 170, 199, 338, 992, 1078
Hpy8I GTNNAC 5 cut(s) 536, 678, 761, 866, 910
HpyAV CCTTC 4 cut(s) 220, 340, 763, 929
HpyCH4III ACNGT 5 cut(s) 256, 277, 322, 628, 1012
HpyCH4IV ACGT 2 cut(s) 862, 1021
HpyF3I CTNAG 4 cut(s) 716, 755, 942, 1064
HpySE526I ACGT 2 cut(s) 862, 1021
Hsp92II CATG 6 cut(s) 372, 458, 526, 701, 908, 1149
HspAI GCGC 6 cut(s) 30, 49, 358, 414, 805, 824
LmnI GCTCC 1 cut(s) 706
Lsp1109I GCAGC 7 cut(s) 97, 139, 250, 789, 970, 973, 1061
LweI GCATC 6 cut(s) 345, 380, 668, 835, 1030, 1036
MaeII ACGT 2 cut(s) 862, 1021
MaeIII GTNAC 3 cut(s) 237, 256, 505
MboII GAAGA 1 cut(s) 1093
MfeI CAATTG 1 cut(s) 726
MhlI GDGCHC 2 cut(s) 763, 858
MlsI TGGCCA 1 cut(s) 1059
MluCI AATT 5 cut(s) 459, 597, 618, 645, 726
MluNI TGGCCA 1 cut(s) 1059
MlyI GAGTC 5 cut(s) 38, 846, 1004, 1093, 1109
MmeI TCCRAC 1 cut(s) 276
Mox20I TGGCCA 1 cut(s) 1059
MscI TGGCCA 1 cut(s) 1059
MseI TTAA 5 cut(s) 159, 279, 447, 648, 924
MslI CAYNNNNRTG 3 cut(s) 527, 574, 861
Msp20I TGGCCA 1 cut(s) 1059
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 4 cut(s) 38, 74, 332, 999
MspR9I CCNGG 2 cut(s) 38, 974
MunI CAATTG 1 cut(s) 726
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 1 cut(s) 974
MvnI CGCG 4 cut(s) 30, 49, 51, 807
NciI CCSGG 1 cut(s) 38
NlaIII CATG 6 cut(s) 372, 458, 526, 701, 908, 1149
NlaIV GGNNCC 3 cut(s) 443, 636, 1034
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 237, 505
NsbI TGCGCA 1 cut(s) 415
NspI RCATGY 3 cut(s) 372, 701, 1149
OliI CACNNNNGTG 2 cut(s) 574, 861
PaeI GCATGC 1 cut(s) 701
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 331
PkrI GCNGC 7 cut(s) 87, 129, 265, 779, 985, 988, 1051
PleI GAGTC 5 cut(s) 38, 845, 1003, 1092, 1108
PmaCI CACGTG 1 cut(s) 863
PmlI CACGTG 1 cut(s) 863
PpsI GAGTC 5 cut(s) 38, 845, 1003, 1092, 1108
Ppu21I YACGTR 1 cut(s) 863
PshAI GACNNNNGTC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 972
PspCI CACGTG 1 cut(s) 863
PspEI GGTNACC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 972
PspN4I GGNNCC 3 cut(s) 443, 636, 1034
PspPI GGNCC 1 cut(s) 1033
RsaI GTAC 2 cut(s) 561, 732
RsaNI GTAC 2 cut(s) 560, 731
RseI CAYNNNNRTG 3 cut(s) 527, 574, 861
SaqAI TTAA 5 cut(s) 159, 279, 447, 648, 924
SatI GCNGC 7 cut(s) 86, 128, 264, 778, 984, 987, 1050
Sau96I GGNCC 1 cut(s) 1033
SchI GAGTC 5 cut(s) 38, 846, 1004, 1093, 1109
ScrFI CCNGG 2 cut(s) 38, 974
SduI GDGCHC 2 cut(s) 763, 858
SfaNI GCATC 6 cut(s) 345, 380, 668, 835, 1030, 1036
SfcI CTRYAG 1 cut(s) 115
SmiMI CAYNNNNRTG 3 cut(s) 527, 574, 861
SmlI CTYRAG 3 cut(s) 209, 446, 551
SmoI CTYRAG 3 cut(s) 209, 446, 551
SphI GCATGC 1 cut(s) 701
Sse9I AATT 5 cut(s) 459, 597, 618, 645, 726
StyD4I CCNGG 2 cut(s) 36, 972
TaaI ACNGT 5 cut(s) 256, 277, 322, 628, 1012
TaiI ACGT 2 cut(s) 865, 1024
TasI AATT 5 cut(s) 459, 597, 618, 645, 726
TatI WGTACW 1 cut(s) 559
Tru1I TTAA 5 cut(s) 159, 279, 447, 648, 924
Tru9I TTAA 5 cut(s) 159, 279, 447, 648, 924
TscAI CASTG 3 cut(s) 280, 581, 893
TseFI GTSAC 2 cut(s) 237, 505
TseI GCWGC 7 cut(s) 85, 127, 263, 777, 983, 986, 1049
Tsp45I GTSAC 2 cut(s) 237, 505
TspDTI ATGAA 8 cut(s) 17, 122, 471, 511, 560, 782, 807, 872
TspRI CASTG 3 cut(s) 280, 581, 893
Vha464I CTTAAG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 759
XceI RCATGY 3 cut(s) 372, 701, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.