RchiOBHm_Chr1g0372941

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015136
Physical Location & Seq
Reverse (-)
61454932 .. 61456061
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59690

Sequence Viewer

Length: 627 bp
ATGGCCACCACCAACCTCTCATTCCCTCACTTCTCTCTTCCATGTCACCACCACCACCACCACCACCACCCCAACTTCTTCACTCTTCCGACTCGGGCCCGACCCGGCCCGAGAACCCAGGCTCTGGAACATGACATGTTTGGAGACTTTGGGGCCAGAGACCCGTTCCCGGCAGAGATAGCCAGTGAGTTTGGAGACAAAGTACTGGGCTTCCAGAACACAGAGCACAAGATCCTCATCCCAACTGTGTCTGCTTCCTCTCTAGCCCAGCAAGAGTGCACCCCTGTCTCTCCTCTCCAGGCTCCTATGCTTGAAGAGGACGCGCAAGAGCTCCTCAGGAAGGTTCTTGGGTGGAGATTGATAGTTGGAGAAGATGGGCTCAAACTACAGTGCTTGTGGAAATTGAGAGATTACCAATGTGGGGTTGAACTCATCAATAGGGTTTATAAGGTTGTGGAAGCTGCAGGGCATTTCCCAAATCTTCACTTGGAGCAACCCAATCAAGTTAGAGCAGAGCTATGGACATCTTCCGTTGGAGGATTGACCATGAATGATTTCATTGTAGCTGCTAAGATAGATGAGATCAAAACATCAGATCTCGTGCCCGTGAGAAGAGCTTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

208

Amino Acids

23.44

Weight (kDa)

6.21

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 102 - 195 2.2e-21 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51110 AT5G51110
fragaria_vesca FvH4_7g26390
malus_domestica MD07G1239200.v1.1
prunus_persica Prupe.2G268000_v2.0.a1 Prupe.2G268000_v2.0.a1
pyrus_communis pycom07g21520
rosa_chinensis RchiOBHm_Chr1g0372941
rosa_laevigata RLG00000026864
rosa_multiflora Rmu_ssc0000425.1_g000015
rosa_roxburghii Rroxscaffold_4G00284590
rosa_rugosa Rorug01G0375100
rosa_samantha Rh1AG383600 Rh1BG347500 Rh1DG378400
rosa_wichuraiana Rw1G033800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 124
AccII CGCG 1 cut(s) 323
AclWI GGATC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 4 cut(s) 124, 169, 340, 421
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 314, 428
AjnI CCWGG 2 cut(s) 117, 297
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 5 cut(s) 331, 461, 517, 566, 617
AluI AGCT 5 cut(s) 331, 461, 517, 566, 617
Alw21I GWGCWC 3 cut(s) 228, 281, 333
Alw26I GTCTC 4 cut(s) 138, 153, 189, 292
Alw44I GTGCAC 1 cut(s) 277
AlwI GGATC 1 cut(s) 226
AlwNI CAGNNNCTG 1 cut(s) 124
Ama87I CYCGRG 2 cut(s) 93, 109
AoxI GGCC 4 cut(s) 3, 96, 106, 153
ApaI GGGCCC 1 cut(s) 100
ApaLI GTGCAC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 461, 566
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 4 cut(s) 96, 97, 107, 153
AsuC2I CCSGG 2 cut(s) 105, 170
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 2 cut(s) 93, 109
AxyI CCTNAGG 1 cut(s) 335
BaeGI GKGCMC 3 cut(s) 100, 281, 606
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 100, 333, 381
BauI CACGAG 1 cut(s) 599
Bbv12I GWGCWC 3 cut(s) 228, 281, 333
BbvI GCAGC 2 cut(s) 448, 553
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 2 cut(s) 119, 299
BcnI CCSGG 2 cut(s) 105, 170
BcoDI GTCTC 4 cut(s) 138, 153, 189, 292
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 386, 462
BglII AGATCT 1 cut(s) 595
BisI GCNGC 2 cut(s) 462, 567
BlsI GCNGC 2 cut(s) 463, 568
BmcAI AGTACT 1 cut(s) 204
Bme1390I CCNGG 4 cut(s) 105, 119, 170, 299
BmeT110I CYCGRG 2 cut(s) 93, 109
BmgT120I GGNCC 4 cut(s) 96, 97, 107, 153
BmiI GGNNCC 3 cut(s) 98, 154, 303
BmrFI CCNGG 4 cut(s) 105, 119, 170, 299
BmrI ACTGGG 1 cut(s) 215
BmuI ACTGGG 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 105, 170
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 169, 340, 421
Bse1I ACTGG 2 cut(s) 183, 210
Bse21I CCTNAGG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 2 cut(s) 119, 299
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 237
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 4 cut(s) 124, 169, 340, 421
BseMII CTCAG 1 cut(s) 349
BseNI ACTGG 2 cut(s) 183, 210
BseRI GAGGAG 2 cut(s) 282, 323
BseSI GKGCMC 3 cut(s) 100, 281, 606
BseXI GCAGC 2 cut(s) 448, 553
BseYI CCCAGC 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 4 cut(s) 5, 98, 108, 155
BsiHKAI GWGCWC 3 cut(s) 228, 281, 333
BsiHKCI CYCGRG 2 cut(s) 93, 109
BsiSI CCGG 2 cut(s) 105, 170
BslI CCNNNNNNNGG 4 cut(s) 124, 169, 340, 421
BsmAI GTCTC 4 cut(s) 138, 153, 189, 292
BsnI GGCC 4 cut(s) 5, 98, 108, 155
Bso31I GGTCTC 1 cut(s) 153
BsoBI CYCGRG 2 cut(s) 93, 109
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 6 cut(s) 100, 228, 281, 333, 381, 606
Bsp143I GATC 3 cut(s) 231, 582, 595
BspANI GGCC 4 cut(s) 5, 98, 108, 155
BspCNI CTCAG 1 cut(s) 348
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 98, 154, 303
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 1 cut(s) 226
BspQI GCTCTTC 1 cut(s) 607
BspTNI GGTCTC 1 cut(s) 153
BsrI ACTGG 2 cut(s) 183, 210
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 3 cut(s) 231, 582, 595
BssSI CACGAG 1 cut(s) 599
Bst2BI CACGAG 1 cut(s) 599
Bst2UI CCWGG 2 cut(s) 119, 299
Bst4CI ACNGT 2 cut(s) 247, 390
Bst6I CTCTTC 4 cut(s) 42, 90, 309, 607
BstDEI CTNAG 3 cut(s) 335, 570, 624
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 1 cut(s) 323
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 3 cut(s) 234, 585, 598
BstMAI GTCTC 4 cut(s) 138, 153, 189, 292
BstMBI GATC 3 cut(s) 231, 582, 595
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 2 cut(s) 119, 299
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 4 cut(s) 103, 117, 168, 297
BstSFI CTRYAG 2 cut(s) 386, 462
BstSLI GKGCMC 3 cut(s) 100, 281, 606
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 448, 553
BstX2I RGATCY 2 cut(s) 231, 595
BstYI RGATCY 2 cut(s) 231, 595
Bsu36I CCTNAGG 1 cut(s) 335
BsuRI GGCC 4 cut(s) 5, 98, 108, 155
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 190, 395
CaiI CAGNNNCTG 1 cut(s) 124
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 4 cut(s) 96, 97, 107, 153
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 203
CviAII CATG 4 cut(s) 42, 131, 136, 547
CviQI GTAC 1 cut(s) 203
DdeI CTNAG 3 cut(s) 335, 570, 624
DpnI GATC 3 cut(s) 233, 584, 597
DpnII GATC 3 cut(s) 231, 582, 595
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 42, 90, 309, 607
EarI CTCTTC 4 cut(s) 42, 90, 309, 607
Ecl136II GAGCTC 1 cut(s) 331
Eco24I GRGCYC 3 cut(s) 100, 333, 381
Eco31I GGTCTC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 331
Eco81I CCTNAGG 1 cut(s) 335
Eco88I CYCGRG 2 cut(s) 93, 109
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoRII CCWGG 2 cut(s) 117, 297
EcoT38I GRGCYC 3 cut(s) 100, 333, 381
FaeI CATG 4 cut(s) 45, 134, 139, 550
FaiI YATR 7 cut(s) 43, 132, 137, 308, 447, 520, 548
FatI CATG 4 cut(s) 41, 130, 135, 546
Fnu4HI GCNGC 2 cut(s) 462, 567
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 3 cut(s) 100, 333, 381
Fsp4HI GCNGC 2 cut(s) 462, 567
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 324
GluI GCNGC 2 cut(s) 462, 567
GsaI CCCAGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 4 cut(s) 5, 98, 108, 155
HapII CCGG 2 cut(s) 105, 170
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 325
Hin1II CATG 4 cut(s) 45, 134, 139, 550
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HinfI GANTC 1 cut(s) 91
HpaII CCGG 2 cut(s) 105, 170
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 2 cut(s) 90, 595
Hpy188III TCNNGA 3 cut(s) 125, 214, 337
Hpy8I GTNNAC 1 cut(s) 279
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 2 cut(s) 247, 390
HpyCH4V TGCA 2 cut(s) 279, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 3 cut(s) 335, 570, 624
Hsp92II CATG 4 cut(s) 45, 134, 139, 550
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 3 cut(s) 231, 582, 595
LguI GCTCTTC 1 cut(s) 607
LmnI GCTCC 3 cut(s) 307, 336, 490
Lsp1109I GCAGC 2 cut(s) 448, 553
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 233, 584, 597
MboI GATC 3 cut(s) 231, 582, 595
MboII GAAGA 8 cut(s) 29, 70, 77, 326, 383, 473, 519, 624
MflI RGATCY 2 cut(s) 231, 595
MhlI GDGCHC 6 cut(s) 100, 228, 281, 333, 381, 606
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 85
MmeI TCCRAC 3 cut(s) 113, 346, 514
MnlI CCTC 8 cut(s) 26, 36, 245, 268, 303, 310, 344, 530
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 105, 170
MspR9I CCNGG 4 cut(s) 105, 119, 170, 299
MvaI CCWGG 2 cut(s) 119, 299
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 2 cut(s) 105, 170
NdeII GATC 3 cut(s) 231, 582, 595
NlaIII CATG 4 cut(s) 45, 134, 139, 550
NlaIV GGNNCC 3 cut(s) 98, 154, 303
NmuCI GTSAC 1 cut(s) 44
NspI RCATGY 1 cut(s) 139
PciI ACATGT 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 2 cut(s) 463, 568
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PscI ACATGT 1 cut(s) 135
PsiI TTATAA 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 117, 297
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 2 cut(s) 117, 297
PspN4I GGNNCC 3 cut(s) 98, 154, 303
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 4 cut(s) 96, 97, 107, 153
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 231, 595
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 607
SatI GCNGC 2 cut(s) 462, 567
Sau3AI GATC 3 cut(s) 231, 582, 595
Sau96I GGNCC 4 cut(s) 96, 97, 107, 153
ScaI AGTACT 1 cut(s) 204
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 4 cut(s) 105, 119, 170, 299
SduI GDGCHC 6 cut(s) 100, 228, 281, 333, 381, 606
SetI ASST 8 cut(s) 18, 333, 345, 453, 463, 519, 568, 619
SfcI CTRYAG 2 cut(s) 386, 462
Sse9I AATT 1 cut(s) 401
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 4 cut(s) 103, 117, 168, 297
TaaI ACNGT 2 cut(s) 247, 390
TasI AATT 1 cut(s) 401
TatI WGTACW 1 cut(s) 202
TscAI CASTG 2 cut(s) 190, 395
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 461, 566
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 547, 563
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 2 cut(s) 190, 395
Van91I CCANNNNNTGG 1 cut(s) 124
VneI GTGCAC 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 338
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 263
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.