Rw1G033800

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015136
Physical Location & Seq
Reverse (-)
61773752 .. 61775277
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033800.1

Sequence Viewer

Length: 630 bp
ATGGCCACCACCAACCTCTCATTCCCTCACTTCTCTCTTCCATGTCACCACCACCACCACCACCACCACCAGCCCAACTTCTTCACTCTTCCGACTCGGGCCCGACCCGGCCCGAGAACCCAGGCTCTGGACCATGACATGTTTGGAGACTTTGGGGCCAGAGACCCGTTCCCGGCAGAGATAGCCAGTGAGTTTGGAGACAAAGTACTGGGCTTCCAGAACACAGAGCACAAGATCCTCATCCCAACTGTGTCTGCTTCCTCTCTAGCCCAGCAAGAGTGCACCCCTGTCTCTCCTCTCCAGGCTCCTATGCTTGAAGAGGACGCGCAACAGCTCCTCAGGAAGGTTCTTGGGTGGAGATTGATAGTTGGAGAAGATGGGCTCAAACTACAGTGCTTGTGGAAATTGAGAGATTACCAATGTGGGGTTGAACTCATCAATAGGGTTTATAAGGTTGTGGAAGCTGCAGGGCATTTCCCAAATCTTCACTTGGAGCAACCCAATCAAGTTAGAGCAGAGCTATGGACATCTTCCGTTGGAGGATTGACCATGAATGATTTCATTGTAGCTGCTAAGATAGATGAGATCAAAACATCAGATCTCGTGCCCGTGAGAAGAGCTTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

209

Amino Acids

23.55

Weight (kDa)

6.34

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 103 - 196 1.8e-21 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51110 AT5G51110
fragaria_vesca FvH4_7g26390
malus_domestica MD07G1239200.v1.1
prunus_persica Prupe.2G268000_v2.0.a1 Prupe.2G268000_v2.0.a1
pyrus_communis pycom07g21520
rosa_chinensis RchiOBHm_Chr1g0372941
rosa_laevigata RLG00000026864
rosa_multiflora Rmu_ssc0000425.1_g000015
rosa_roxburghii Rroxscaffold_4G00284590
rosa_rugosa Rorug01G0375100
rosa_samantha Rh1AG383600 Rh1BG347500 Rh1DG378400
rosa_wichuraiana Rw1G033800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AccB7I CCANNNNNTGG 1 cut(s) 127
AccII CGCG 1 cut(s) 326
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 207
AfiI CCNNNNNNNGG 4 cut(s) 127, 172, 343, 424
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 2 cut(s) 317, 431
AjnI CCWGG 2 cut(s) 120, 300
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 5 cut(s) 334, 464, 520, 569, 620
AluI AGCT 5 cut(s) 334, 464, 520, 569, 620
Alw21I GWGCWC 2 cut(s) 231, 284
Alw26I GTCTC 4 cut(s) 141, 156, 192, 295
Alw44I GTGCAC 1 cut(s) 280
AlwI GGATC 1 cut(s) 229
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 2 cut(s) 96, 112
AoxI GGCC 4 cut(s) 3, 99, 109, 156
ApaI GGGCCC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 464, 569
Asp700I GAANNNNTTC 1 cut(s) 557
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 5 cut(s) 99, 100, 110, 130, 156
AsuC2I CCSGG 2 cut(s) 108, 173
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 2 cut(s) 96, 112
AvaII GGWCC 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 338
BaeGI GKGCMC 3 cut(s) 103, 284, 609
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 103, 384
BauI CACGAG 1 cut(s) 602
Bbv12I GWGCWC 2 cut(s) 231, 284
BbvI GCAGC 2 cut(s) 451, 556
BccI CCATC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 122, 302
BcnI CCSGG 2 cut(s) 108, 173
BcoDI GTCTC 4 cut(s) 141, 156, 192, 295
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 389, 465
BglII AGATCT 1 cut(s) 598
BisI GCNGC 2 cut(s) 465, 570
BlsI GCNGC 2 cut(s) 466, 571
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 4 cut(s) 108, 122, 173, 302
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 96, 112
BmgT120I GGNCC 5 cut(s) 99, 100, 110, 130, 156
BmiI GGNNCC 3 cut(s) 101, 157, 306
BmrFI CCNGG 4 cut(s) 108, 122, 173, 302
BmrI ACTGGG 1 cut(s) 218
BmuI ACTGGG 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 108, 173
BsaBI GATNNNNATC 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 172, 343, 424
Bse1I ACTGG 2 cut(s) 186, 213
Bse21I CCTNAGG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 2 cut(s) 122, 302
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 4 cut(s) 127, 172, 343, 424
BseMII CTCAG 1 cut(s) 352
BseNI ACTGG 2 cut(s) 186, 213
BseRI GAGGAG 2 cut(s) 285, 326
BseSI GKGCMC 3 cut(s) 103, 284, 609
BseXI GCAGC 2 cut(s) 451, 556
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 4 cut(s) 5, 101, 111, 158
BsiHKAI GWGCWC 2 cut(s) 231, 284
BsiHKCI CYCGRG 2 cut(s) 96, 112
BsiSI CCGG 2 cut(s) 108, 173
BslI CCNNNNNNNGG 4 cut(s) 127, 172, 343, 424
BsmAI GTCTC 4 cut(s) 141, 156, 192, 295
BsnI GGCC 4 cut(s) 5, 101, 111, 158
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 2 cut(s) 96, 112
Bsp120I GGGCCC 1 cut(s) 99
Bsp1286I GDGCHC 5 cut(s) 103, 231, 284, 384, 609
Bsp143I GATC 3 cut(s) 234, 585, 598
BspANI GGCC 4 cut(s) 5, 101, 111, 158
BspCNI CTCAG 1 cut(s) 351
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 3 cut(s) 101, 157, 306
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 1 cut(s) 610
BspTNI GGTCTC 1 cut(s) 156
BsrI ACTGG 2 cut(s) 186, 213
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 3 cut(s) 234, 585, 598
BssSI CACGAG 1 cut(s) 602
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 2 cut(s) 122, 302
Bst4CI ACNGT 2 cut(s) 250, 393
Bst6I CTCTTC 4 cut(s) 42, 93, 312, 610
BstDEI CTNAG 3 cut(s) 338, 573, 627
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 237, 588, 601
BstMAI GTCTC 4 cut(s) 141, 156, 192, 295
BstMBI GATC 3 cut(s) 234, 585, 598
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 2 cut(s) 122, 302
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 4 cut(s) 106, 120, 171, 300
BstSFI CTRYAG 2 cut(s) 389, 465
BstSLI GKGCMC 3 cut(s) 103, 284, 609
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 2 cut(s) 451, 556
BstX2I RGATCY 2 cut(s) 234, 598
BstYI RGATCY 2 cut(s) 234, 598
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 4 cut(s) 5, 101, 111, 158
BtsCI GGATG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 193, 398
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 5 cut(s) 99, 100, 110, 130, 156
CseI GACGC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 206
CviAII CATG 4 cut(s) 42, 134, 139, 550
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 3 cut(s) 338, 573, 627
DpnI GATC 3 cut(s) 236, 587, 600
DpnII GATC 3 cut(s) 234, 585, 598
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 42, 93, 312, 610
EarI CTCTTC 4 cut(s) 42, 93, 312, 610
Eco24I GRGCYC 2 cut(s) 103, 384
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 338
Eco88I CYCGRG 2 cut(s) 96, 112
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 120, 300
EcoT38I GRGCYC 2 cut(s) 103, 384
FaeI CATG 4 cut(s) 45, 137, 142, 553
FaiI YATR 7 cut(s) 43, 135, 140, 311, 450, 523, 551
FatI CATG 4 cut(s) 41, 133, 138, 549
Fnu4HI GCNGC 2 cut(s) 465, 570
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 2 cut(s) 103, 384
Fsp4HI GCNGC 2 cut(s) 465, 570
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 465, 570
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 4 cut(s) 5, 101, 111, 158
HapII CCGG 2 cut(s) 108, 173
HgaI GACGC 1 cut(s) 332
HhaI GCGC 1 cut(s) 328
Hin1II CATG 4 cut(s) 45, 137, 142, 553
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 1 cut(s) 94
HpaII CCGG 2 cut(s) 108, 173
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 2 cut(s) 93, 598
Hpy188III TCNNGA 3 cut(s) 128, 217, 340
Hpy8I GTNNAC 1 cut(s) 282
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 2 cut(s) 250, 393
HpyCH4V TGCA 2 cut(s) 282, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 3 cut(s) 338, 573, 627
Hsp92II CATG 4 cut(s) 45, 137, 142, 553
HspAI GCGC 1 cut(s) 326
Kzo9I GATC 3 cut(s) 234, 585, 598
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 3 cut(s) 310, 339, 493
Lsp1109I GCAGC 2 cut(s) 451, 556
MaeI CTAG 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 236, 587, 600
MboI GATC 3 cut(s) 234, 585, 598
MboII GAAGA 8 cut(s) 29, 73, 80, 329, 386, 476, 522, 627
MflI RGATCY 2 cut(s) 234, 598
MhlI GDGCHC 5 cut(s) 103, 231, 284, 384, 609
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 404
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 3 cut(s) 116, 349, 517
MnlI CCTC 8 cut(s) 26, 36, 248, 271, 306, 313, 347, 533
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 557
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 108, 173
MspR9I CCNGG 4 cut(s) 108, 122, 173, 302
MvaI CCWGG 2 cut(s) 122, 302
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 2 cut(s) 108, 173
NdeII GATC 3 cut(s) 234, 585, 598
NlaIII CATG 4 cut(s) 45, 137, 142, 553
NlaIV GGNNCC 3 cut(s) 101, 157, 306
NmuCI GTSAC 1 cut(s) 44
NspI RCATGY 1 cut(s) 142
PciI ACATGT 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 557
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 2 cut(s) 466, 571
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PscI ACATGT 1 cut(s) 138
PsiI TTATAA 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 120, 300
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 120, 300
PspN4I GGNNCC 3 cut(s) 101, 157, 306
PspOMI GGGCCC 1 cut(s) 99
PspPI GGNCC 5 cut(s) 99, 100, 110, 130, 156
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 2 cut(s) 234, 598
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 610
SatI GCNGC 2 cut(s) 465, 570
Sau3AI GATC 3 cut(s) 234, 585, 598
Sau96I GGNCC 5 cut(s) 99, 100, 110, 130, 156
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 4 cut(s) 108, 122, 173, 302
SduI GDGCHC 5 cut(s) 103, 231, 284, 384, 609
SetI ASST 8 cut(s) 18, 336, 348, 456, 466, 522, 571, 622
SfcI CTRYAG 2 cut(s) 389, 465
SinI GGWCC 1 cut(s) 130
Sse9I AATT 1 cut(s) 404
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 4 cut(s) 106, 120, 171, 300
TaaI ACNGT 2 cut(s) 250, 393
TasI AATT 1 cut(s) 404
TatI WGTACW 1 cut(s) 205
TscAI CASTG 2 cut(s) 193, 398
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 464, 569
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 2 cut(s) 550, 566
TspGWI ACGGA 1 cut(s) 523
TspRI CASTG 2 cut(s) 193, 398
Van91I CCANNNNNTGG 1 cut(s) 127
VneI GTGCAC 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 341
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 557
XspI CTAG 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.