Rroxscaffold_4G00284590

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015136
Physical Location & Seq
Forward (+)
6168024 .. 6170780
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284590.1

Sequence Viewer

Length: 831 bp
ATGAAAATGGTGATGGTGATCAGGGCCTTGATGACGATATACTTAGTTTCTTGCTTGGCGAATGTTTTGGATTTGGTTCGGAAGGTGCAGGCGGACACGACGCCGAGGCAGTGCAAGCAGGAGAAGAAGCTTCTCGTGAACGAGTGCAAGGCGCTTATTCTTGGGGCCAATAAGCTCGCTAATTTGATCAATGTCCCTAGCCTCGTTAAGAAGATTGTGGAAGTATCACCACTCCACCATAAGATGGAAGACATGCATGCGTGTACTGATTCTAGGATTGAATTCTCGACTTCACCACTGATAACGAACACTTTTATTAGTGAAGCTCTGGGCCATGACATGATTGGAGACTTTGGGGCCAGAGACCCGTTCCCGGCAGAGATAGCCAGTGAGTTTGGAGACAAAGTACTGGGCTTCCAGAACACAGAGCACAAGATCCTCATCCCAACTGTGTCTGCTTCCTCTCTAGCCCAGCAAGAGTGCACCCCTGTCTCTCCTCTCCAGGCTCCTATGCTTGAAGAGGACGCGCAACAGCTCCTCAGGAAGGTTCTTGGGTGGAGATTGATAGTTGGAGAAGATGGGCTCAAACTACAGTGCTTGTGGAAATTGAGAGATTACCAATGTGGGGTTGAACTCATCAATAGGGTTTATAAGGTTGTTGAAGCTGCAGGGCATTTCCCAAATCTTCACTTGGAGCAACCCAATCAAGTTAGAGCAGAGCTATGGACATCTTCCGTTGGAGGATTGACCATGAATGATTTCATTGTAGCTGCTAAAATAGATGCGATCAAAACATCGGATCTCGTGCCCATGAGAAGAGCTTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

276

Amino Acids

30.74

Weight (kDa)

6.66

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 170 - 263 4.8e-21 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51110 AT5G51110
fragaria_vesca FvH4_7g26390
malus_domestica MD07G1239200.v1.1
prunus_persica Prupe.2G268000_v2.0.a1 Prupe.2G268000_v2.0.a1
pyrus_communis pycom07g21520
rosa_chinensis RchiOBHm_Chr1g0372941
rosa_laevigata RLG00000026864
rosa_multiflora Rmu_ssc0000425.1_g000015
rosa_roxburghii Rroxscaffold_4G00284590
rosa_rugosa Rorug01G0375100
rosa_samantha Rh1AG383600 Rh1BG347500 Rh1DG378400
rosa_wichuraiana Rw1G033800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AccB7I CCANNNNNTGG 1 cut(s) 244
AccII CGCG 1 cut(s) 527
AciI CCGC 1 cut(s) 92
AclWI GGATC 2 cut(s) 430, 807
AcsI RAATTY 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 2 cut(s) 265, 408
AfiI CCNNNNNNNGG 4 cut(s) 244, 373, 544, 625
AgsI TTSAA 4 cut(s) 281, 518, 632, 662
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 8 cut(s) 130, 175, 326, 535, 665, 721, 770, 821
AluI AGCT 8 cut(s) 130, 175, 326, 535, 665, 721, 770, 821
Alw21I GWGCWC 2 cut(s) 432, 485
Alw26I GTCTC 4 cut(s) 342, 357, 393, 496
Alw44I GTGCAC 1 cut(s) 481
AlwI GGATC 2 cut(s) 430, 807
AoxI GGCC 4 cut(s) 24, 165, 331, 357
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 2 cut(s) 665, 770
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 758
AspLEI GCGC 2 cut(s) 154, 529
AspS9I GGNCC 4 cut(s) 24, 165, 331, 357
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 4 cut(s) 22, 28, 219, 285
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 2 cut(s) 485, 810
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 2 cut(s) 134, 803
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 432, 485
BbvI GCAGC 2 cut(s) 652, 757
BccI CCATC 3 cut(s) 7, 238, 572
BciT130I CCWGG 1 cut(s) 503
BclI TGATCA 2 cut(s) 18, 186
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 4 cut(s) 342, 357, 393, 496
BfaI CTAG 3 cut(s) 198, 273, 467
BfmI CTRYAG 2 cut(s) 590, 666
BfoI RGCGCY 1 cut(s) 155
BisI GCNGC 2 cut(s) 666, 771
BlsI GCNGC 2 cut(s) 667, 772
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 2 cut(s) 374, 503
BmgT120I GGNCC 4 cut(s) 24, 165, 331, 357
BmiI GGNNCC 3 cut(s) 166, 358, 507
BmrFI CCNGG 2 cut(s) 374, 503
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 772
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 374
BsaBI GATNNNNATC 2 cut(s) 17, 440
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 373, 544, 625
Bse1I ACTGG 2 cut(s) 387, 414
Bse21I CCTNAGG 1 cut(s) 539
Bse8I GATNNNNATC 2 cut(s) 17, 440
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 1 cut(s) 441
BseJI GATNNNNATC 2 cut(s) 17, 440
BseLI CCNNNNNNNGG 4 cut(s) 244, 373, 544, 625
BseMII CTCAG 1 cut(s) 553
BseNI ACTGG 2 cut(s) 387, 414
BseRI GAGGAG 2 cut(s) 486, 527
BseSI GKGCMC 2 cut(s) 485, 810
BseXI GCAGC 2 cut(s) 652, 757
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 4 cut(s) 26, 167, 333, 359
BsiHKAI GWGCWC 2 cut(s) 432, 485
BsiSI CCGG 1 cut(s) 374
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 4 cut(s) 244, 373, 544, 625
BsmAI GTCTC 4 cut(s) 342, 357, 393, 496
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 26, 167, 333, 359
Bso31I GGTCTC 1 cut(s) 357
Bsp1286I GDGCHC 4 cut(s) 432, 485, 585, 810
Bsp143I GATC 5 cut(s) 18, 186, 435, 786, 799
BspACI CCGC 1 cut(s) 92
BspANI GGCC 4 cut(s) 26, 167, 333, 359
BspCNI CTCAG 1 cut(s) 552
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 3 cut(s) 166, 358, 507
BspMAI CTGCAG 1 cut(s) 670
BspPI GGATC 2 cut(s) 430, 807
BspQI GCTCTTC 1 cut(s) 811
BspTNI GGTCTC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 387, 414
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 5 cut(s) 18, 186, 435, 786, 799
BssNI GRCGYC 1 cut(s) 101
BssSI CACGAG 2 cut(s) 134, 803
Bst2BI CACGAG 2 cut(s) 134, 803
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 451, 594
Bst6I CTCTTC 2 cut(s) 513, 811
BstACI GRCGYC 1 cut(s) 101
BstC8I GCNNGC 4 cut(s) 90, 116, 177, 258
BstDEI CTNAG 3 cut(s) 43, 539, 828
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 441
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 2 cut(s) 154, 529
BstKTI GATC 5 cut(s) 21, 189, 438, 789, 802
BstMAI GTCTC 4 cut(s) 342, 357, 393, 496
BstMBI GATC 5 cut(s) 18, 186, 435, 786, 799
BstMWI GCNNNNNNNGC 2 cut(s) 115, 383
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 2 cut(s) 256, 260
BstSCI CCNGG 2 cut(s) 372, 501
BstSFI CTRYAG 2 cut(s) 590, 666
BstSLI GKGCMC 2 cut(s) 485, 810
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 652, 757
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 2 cut(s) 435, 799
BstYI RGATCY 2 cut(s) 435, 799
Bsu36I CCTNAGG 1 cut(s) 539
BsuRI GGCC 4 cut(s) 26, 167, 333, 359
BtsCI GGATG 1 cut(s) 441
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 4 cut(s) 116, 296, 394, 599
Cac8I GCNNGC 4 cut(s) 90, 116, 177, 258
CfoI GCGC 2 cut(s) 154, 529
Cfr13I GGNCC 4 cut(s) 24, 165, 331, 357
CseI GACGC 2 cut(s) 109, 533
Csp6I GTAC 2 cut(s) 264, 407
CviAII CATG 6 cut(s) 253, 257, 335, 340, 751, 811
CviQI GTAC 2 cut(s) 264, 407
DdeI CTNAG 3 cut(s) 43, 539, 828
DpnI GATC 5 cut(s) 20, 188, 437, 788, 801
DpnII GATC 5 cut(s) 18, 186, 435, 786, 799
Eam1104I CTCTTC 2 cut(s) 513, 811
EarI CTCTTC 2 cut(s) 513, 811
EciI GGCGGA 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 357
Eco81I CCTNAGG 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 585
FaeI CATG 6 cut(s) 256, 260, 338, 343, 754, 814
FaqI GGGAC 1 cut(s) 179
FatI CATG 6 cut(s) 252, 256, 334, 339, 750, 810
FbaI TGATCA 2 cut(s) 18, 186
Fnu4HI GCNGC 2 cut(s) 666, 771
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 2 cut(s) 666, 771
FspBI CTAG 3 cut(s) 198, 273, 467
GlaI GCGC 2 cut(s) 153, 528
GluI GCNGC 2 cut(s) 666, 771
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 4 cut(s) 26, 167, 333, 359
HapII CCGG 1 cut(s) 374
HgaI GACGC 2 cut(s) 109, 533
HhaI GCGC 2 cut(s) 154, 529
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 6 cut(s) 256, 260, 338, 343, 754, 814
Hin6I GCGC 2 cut(s) 152, 527
HinP1I GCGC 2 cut(s) 152, 527
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 374
HphI GGTGA 4 cut(s) 22, 28, 219, 285
Hpy166II GTNNAC 3 cut(s) 139, 264, 483
Hpy188I TCNGA 2 cut(s) 81, 799
Hpy188III TCNNGA 4 cut(s) 136, 286, 418, 541
Hpy8I GTNNAC 3 cut(s) 139, 264, 483
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 76, 538
HpyCH4III ACNGT 2 cut(s) 451, 594
HpyCH4V TGCA 6 cut(s) 88, 114, 147, 256, 483, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 383
HpyF3I CTNAG 3 cut(s) 43, 539, 828
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 6 cut(s) 256, 260, 338, 343, 754, 814
HspAI GCGC 2 cut(s) 152, 527
Ksp22I TGATCA 2 cut(s) 18, 186
Kzo9I GATC 5 cut(s) 18, 186, 435, 786, 799
LguI GCTCTTC 1 cut(s) 811
LmnI GCTCC 3 cut(s) 511, 540, 694
Lsp1109I GCAGC 2 cut(s) 652, 757
LweI GCATC 1 cut(s) 772
MaeI CTAG 3 cut(s) 198, 273, 467
MalI GATC 5 cut(s) 20, 188, 437, 788, 801
MboI GATC 5 cut(s) 18, 186, 435, 786, 799
MboII GAAGA 8 cut(s) 136, 223, 260, 530, 587, 677, 723, 828
MflI RGATCY 2 cut(s) 435, 799
MhlI GDGCHC 4 cut(s) 432, 485, 585, 810
MluCI AATT 3 cut(s) 181, 281, 605
MmeI TCCRAC 2 cut(s) 550, 718
MnlI CCTC 8 cut(s) 99, 212, 449, 472, 507, 514, 548, 734
Mph1103I ATGCAT 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 758
MseI TTAA 1 cut(s) 207
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 2 cut(s) 374, 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 2 cut(s) 115, 383
NciI CCSGG 1 cut(s) 374
NdeII GATC 5 cut(s) 18, 186, 435, 786, 799
NlaIII CATG 6 cut(s) 256, 260, 338, 343, 754, 814
NlaIV GGNNCC 3 cut(s) 166, 358, 507
NmeAIII GCCGAG 1 cut(s) 129
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 2 cut(s) 256, 260
PaeI GCATGC 1 cut(s) 260
PciSI GCTCTTC 1 cut(s) 811
PdmI GAANNNNTTC 1 cut(s) 758
PfeI GAWTC 1 cut(s) 269
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 2 cut(s) 667, 772
PsiI TTATAA 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 501
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 3 cut(s) 166, 358, 507
PspPI GGNCC 4 cut(s) 24, 165, 331, 357
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 2 cut(s) 435, 799
RsaI GTAC 2 cut(s) 265, 408
RsaNI GTAC 2 cut(s) 264, 407
SapI GCTCTTC 1 cut(s) 811
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 2 cut(s) 666, 771
Sau3AI GATC 5 cut(s) 18, 186, 435, 786, 799
Sau96I GGNCC 4 cut(s) 24, 165, 331, 357
ScaI AGTACT 1 cut(s) 408
ScrFI CCNGG 2 cut(s) 374, 503
SduI GDGCHC 4 cut(s) 432, 485, 585, 810
SfaNI GCATC 1 cut(s) 772
SfcI CTRYAG 2 cut(s) 590, 666
SphI GCATGC 1 cut(s) 260
Sse9I AATT 3 cut(s) 181, 281, 605
SsiI CCGC 1 cut(s) 92
SspMI CTAG 3 cut(s) 198, 273, 467
StyD4I CCNGG 2 cut(s) 372, 501
TaaI ACNGT 2 cut(s) 451, 594
TaqI TCGA 1 cut(s) 287
TasI AATT 3 cut(s) 181, 281, 605
TatI WGTACW 2 cut(s) 263, 406
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 4 cut(s) 116, 303, 394, 599
TseI GCWGC 2 cut(s) 665, 770
TspDTI ATGAA 3 cut(s) 17, 751, 767
TspGWI ACGGA 1 cut(s) 724
TspRI CASTG 4 cut(s) 116, 303, 394, 599
Van91I CCANNNNNTGG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 481
XagI CCTNNNNNAGG 1 cut(s) 542
XapI RAATTY 1 cut(s) 281
XceI RCATGY 2 cut(s) 256, 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 758
XspI CTAG 3 cut(s) 198, 273, 467
ZrmI AGTACT 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.