Rorug01G0375100

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015136
Physical Location & Seq
Reverse (-)
48919685 .. 48923201
3517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0375100.1

Sequence Viewer

Length: 1317 bp
ATGGCCACCGCCGTCTCCACCATCGGAGCTGTCAACAGAGTCTCGCTGAGCTTAAATGGCTCTAGCGGCAGTGCTTCAGTTCCAAGCTCAAGCTTTATGGGAAGCAGCTTGAAGAAAGTGAACTCAAGGTTCCCCAACTCCAAAGTCTCATCCGGGAGCTTCAAGATTGTAGCAGAGGCAGTCGATGAAGACAAGCAGACCGAAAAGGACAAATGGAAAGGCCTTGCCTTTGACACCTCCGATGACCAACAAGACATCACCAGAGGAAAGGGTATGGTTGATACCCTCTTCCAAGCTCCAGTGGGCGACGGAACTCACATGGCCATCATGAGTTCTTATGACTATGTCAGCACTGGACTTCGCACGTACGACATGGACAACATGAAGGATGGTTTCTACATTGCTCCTGCTTTCATGGACAAGCTTGTTGTTCACATCACAAAGAACTTCATGAGCTTGCCTAACATCAAGGTTCCCCTTATCCTTGGTATTTGGGGAGGCAAAGGTCAGGGTAAATCTTTCCAGTGTGAGCTTGTGTTTGCTAAGATGGGAATCAACCCTATCATGATGAGTGCCGGAGAATTGGAAAGTGGAAACGCTGGAGAACCCGCGAAGTTGATCAGGCAAAGGTACCGTGAGGCAGCCGATATCATCAGGAAGGGGAAAATGTGCGCCCTCTTCATCAACGATCTTGATGCAGGAGCTGGTCGTATGGGTGGAACCACCCAATACACTGTGAACAACCAGATGGTTAACGCCACCCTCATGAACATTGCTGATAACCCAACCAATGTCCAGCTCCCCGGTATGTACAACAAGGAGGATAACCCCCGTGTCCCCATCATTGTCACCGGTAACGATTTCTCAACATTGTATGCTCCTCTCATCCGTGATGGGCGTATGGAGAAGTTCTACTGGGCTCCTACAAGAGAAGATCGTATTGGTGTCTGCATTGGTATCTTCAAATCTGACAATGTTGCCCAATCCGATATTGTCAAGCTAGTTGACACCTTCCCCGGCCAATCCATCGATTTCTTTGGTGCCCTTAGGGCCAGAGTTTACGATGATGAAGTGAGGAAGTGGATTTCCGGTGTTGGTGTGGACACCATTGGAAAGAAGCTTGTGAACTCAAAGGAAGGACCCCCGACTTTCGAGCAGCCCAAGATGACAATTGAGAAGCTCCTTGAGTACGGAAACATGCTTGTGCAAGAGCAAGAGAATGTGAAGAGAGTCCAATTGGCCGACCAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAACTCTGATGCCATTGATAGAGGAACTTTCTTCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

438

Amino Acids

47.92

Weight (kDa)

5.7

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 164 - 307 7.6e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 310 - 405 7.4e-41 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51110 AT5G51110
fragaria_vesca FvH4_7g26390
malus_domestica MD07G1239200.v1.1
prunus_persica Prupe.2G268000_v2.0.a1 Prupe.2G268000_v2.0.a1
pyrus_communis pycom07g21520
rosa_chinensis RchiOBHm_Chr1g0372941
rosa_laevigata RLG00000026864
rosa_multiflora Rmu_ssc0000425.1_g000015
rosa_roxburghii Rroxscaffold_4G00284590
rosa_rugosa Rorug01G0375100
rosa_samantha Rh1AG383600 Rh1BG347500 Rh1DG378400
rosa_wichuraiana Rw1G033800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 630
AccB1I GGYRCC 2 cut(s) 630, 1040
AccII CGCG 1 cut(s) 611
AciI CCGC 3 cut(s) 9, 66, 609
AcoI YGGCCR 4 cut(s) 3, 321, 1018, 1239
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 5 cut(s) 368, 632, 812, 1190, 1250
AgeI ACCGGT 1 cut(s) 851
AgsI TTSAA 3 cut(s) 112, 163, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 924, 956
Alw26I GTCTC 3 cut(s) 19, 46, 151
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 6 cut(s) 3, 220, 321, 1018, 1050, 1239
ApeKI GCWGC 4 cut(s) 105, 641, 1156, 1262
AsiGI ACCGGT 1 cut(s) 851
Asp718I GGTACC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 1050, 1139
AsuC2I CCSGG 3 cut(s) 154, 804, 1017
AsuHPI GGTGA 3 cut(s) 250, 841, 1283
AvaII GGWCC 1 cut(s) 1139
AxyI CCTNAGG 1 cut(s) 1046
BaeGI GKGCMC 1 cut(s) 1045
BalI TGGCCA 2 cut(s) 5, 323
BanI GGYRCC 2 cut(s) 630, 1040
BanII GRGCYC 1 cut(s) 922
BbsI GAAGAC 1 cut(s) 195
BbvI GCAGC 4 cut(s) 117, 653, 1168, 1249
BccI CCATC 8 cut(s) 29, 332, 383, 541, 742, 848, 887, 1034
BcgI CGANNNNNNTGC 2 cut(s) 677, 711
BclI TGATCA 1 cut(s) 618
BcnI CCSGG 3 cut(s) 154, 804, 1017
BcoDI GTCTC 3 cut(s) 19, 46, 151
BfaI CTAG 2 cut(s) 63, 1001
BglI GCCNNNNNGGC 1 cut(s) 1049
BisI GCNGC 5 cut(s) 67, 106, 642, 1157, 1263
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 5 cut(s) 68, 107, 643, 1158, 1264
BmcAI AGTACT 1 cut(s) 1250
Bme1390I CCNGG 3 cut(s) 154, 804, 1017
Bme18I GGWCC 1 cut(s) 1139
BmgT120I GGNCC 2 cut(s) 1050, 1139
BmiI GGNNCC 7 cut(s) 131, 474, 632, 721, 921, 1042, 1141
BmrFI CCNGG 3 cut(s) 154, 804, 1017
BmrI ACTGGG 1 cut(s) 925
BmsI GCATC 3 cut(s) 685, 1264, 1276
BmuI ACTGGG 1 cut(s) 925
BpiI GAAGAC 1 cut(s) 195
BpmI CTGGAG 2 cut(s) 282, 621
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 4 cut(s) 73, 109, 1205, 1273
BpuMI CCSGG 3 cut(s) 154, 804, 1017
Bsa29I ATCGAT 1 cut(s) 1029
BsaAI YACGTR 1 cut(s) 366
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 484, 802, 1015
BsaWI WCCGGW 2 cut(s) 851, 1088
Bse118I RCCGGY 1 cut(s) 851
Bse1I ACTGG 5 cut(s) 299, 358, 523, 920, 1246
Bse21I CCTNAGG 1 cut(s) 1046
Bse3DI GCAATG 2 cut(s) 399, 771
Bse8I GATNNNNATC 1 cut(s) 551
BseCI ATCGAT 1 cut(s) 1029
BseDI CCNNGG 3 cut(s) 484, 802, 1015
BseGI GGATG 3 cut(s) 149, 394, 885
BseJI GATNNNNATC 1 cut(s) 551
BseMI GCAATG 2 cut(s) 399, 771
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 5 cut(s) 299, 358, 523, 920, 1246
BseRI GAGGAG 1 cut(s) 870
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 4 cut(s) 117, 653, 1168, 1249
Bsh1236I CGCG 1 cut(s) 611
BshFI GGCC 6 cut(s) 5, 222, 323, 1020, 1052, 1241
BshNI GGYRCC 2 cut(s) 630, 1040
BshTI ACCGGT 1 cut(s) 851
BshVI ATCGAT 1 cut(s) 1029
BsiSI CCGG 6 cut(s) 153, 576, 804, 852, 1017, 1089
BsiWI CGTACG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 821
BsmAI GTCTC 3 cut(s) 19, 46, 151
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 821
BsnI GGCC 6 cut(s) 5, 222, 323, 1020, 1052, 1241
Bsp1286I GDGCHC 2 cut(s) 922, 1045
Bsp1407I TGTACA 1 cut(s) 810
Bsp143I GATC 3 cut(s) 618, 688, 934
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 3 cut(s) 9, 66, 609
BspANI GGCC 6 cut(s) 5, 222, 323, 1020, 1052, 1241
BspCNI CTCAG 1 cut(s) 39
BspDI ATCGAT 1 cut(s) 1029
BspFNI CGCG 1 cut(s) 611
BspHI TCATGA 4 cut(s) 327, 450, 564, 765
BspLI GGNNCC 7 cut(s) 131, 474, 632, 721, 921, 1042, 1141
BspT107I GGYRCC 2 cut(s) 630, 1040
BsrDI GCAATG 2 cut(s) 399, 771
BsrFI RCCGGY 1 cut(s) 851
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 5 cut(s) 299, 358, 523, 920, 1246
BssAI RCCGGY 1 cut(s) 851
BssECI CCNNGG 3 cut(s) 484, 802, 1015
BssMI GATC 3 cut(s) 618, 688, 934
BssT1I CCWWGG 1 cut(s) 484
Bst4CI ACNGT 2 cut(s) 635, 736
Bst6I CTCTTC 3 cut(s) 293, 683, 1220
BstAUI TGTACA 1 cut(s) 810
BstBAI YACGTR 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 3 cut(s) 47, 543, 1046
BstF5I GGATG 3 cut(s) 149, 394, 885
BstFNI CGCG 1 cut(s) 611
BstHHI GCGC 1 cut(s) 674
BstKTI GATC 3 cut(s) 621, 691, 937
BstMAI GTCTC 3 cut(s) 19, 46, 151
BstMBI GATC 3 cut(s) 618, 688, 934
BstMWI GCNNNNNNNGC 3 cut(s) 57, 66, 1049
BstNSI RCATGY 1 cut(s) 1201
BstSCI CCNGG 3 cut(s) 152, 802, 1015
BstSLI GKGCMC 1 cut(s) 1045
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 4 cut(s) 117, 653, 1168, 1249
BstV2I GAAGAC 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 1029
Bsu36I CCTNAGG 1 cut(s) 1046
BsuRI GGCC 6 cut(s) 5, 222, 323, 1020, 1052, 1241
BsuTUI ATCGAT 1 cut(s) 1029
BtsCI GGATG 3 cut(s) 149, 394, 885
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 5 cut(s) 76, 306, 351, 530, 732
Cac8I GCNNGC 1 cut(s) 458
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 4 cut(s) 327, 450, 564, 765
CfoI GCGC 1 cut(s) 674
Cfr10I RCCGGY 1 cut(s) 851
Cfr13I GGNCC 2 cut(s) 1050, 1139
ClaI ATCGAT 1 cut(s) 1029
Csp6I GTAC 5 cut(s) 367, 631, 811, 1189, 1249
CspAI ACCGGT 1 cut(s) 851
CviAII CATG 9 cut(s) 319, 328, 373, 382, 415, 451, 565, 766, 1198
CviQI GTAC 5 cut(s) 367, 631, 811, 1189, 1249
DdeI CTNAG 3 cut(s) 47, 543, 1046
DpnI GATC 3 cut(s) 620, 690, 936
DpnII GATC 3 cut(s) 618, 688, 934
EaeI YGGCCR 4 cut(s) 3, 321, 1018, 1239
Eam1104I CTCTTC 3 cut(s) 293, 683, 1220
EarI CTCTTC 3 cut(s) 293, 683, 1220
Eco130I CCWWGG 1 cut(s) 484
Eco147I AGGCCT 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 922
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 1139
Eco57I CTGAAG 1 cut(s) 60
Eco81I CCTNAGG 1 cut(s) 1046
EcoO109I RGGNCCY 1 cut(s) 1139
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 484
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 9 cut(s) 322, 331, 376, 385, 418, 454, 568, 769, 1201
FaqI GGGAC 1 cut(s) 821
FatI CATG 9 cut(s) 318, 327, 372, 381, 414, 450, 564, 765, 1197
FauI CCCGC 1 cut(s) 616
FbaI TGATCA 1 cut(s) 618
Fnu4HI GCNGC 5 cut(s) 67, 106, 642, 1157, 1263
FokI GGATG 3 cut(s) 136, 401, 872
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 5 cut(s) 67, 106, 642, 1157, 1263
FspBI CTAG 2 cut(s) 63, 1001
GlaI GCGC 1 cut(s) 673
GluI GCNGC 5 cut(s) 67, 106, 642, 1157, 1263
GsuI CTGGAG 2 cut(s) 282, 621
HaeIII GGCC 6 cut(s) 5, 222, 323, 1020, 1052, 1241
HapII CCGG 6 cut(s) 153, 576, 804, 852, 1017, 1089
HhaI GCGC 1 cut(s) 674
Hin1II CATG 9 cut(s) 322, 331, 376, 385, 418, 454, 568, 769, 1201
Hin6I GCGC 1 cut(s) 672
HinP1I GCGC 1 cut(s) 672
HincII GTYRAC 3 cut(s) 34, 754, 1006
HindII GTYRAC 3 cut(s) 34, 754, 1006
HindIII AAGCTT 3 cut(s) 91, 422, 1118
HinfI GANTC 3 cut(s) 39, 552, 1230
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 6 cut(s) 153, 576, 804, 852, 1017, 1089
HphI GGTGA 3 cut(s) 250, 841, 1283
Hpy166II GTNNAC 9 cut(s) 34, 121, 433, 739, 754, 1006, 1060, 1102, 1126
Hpy188I TCNGA 5 cut(s) 26, 241, 970, 988, 1285
Hpy188III TCNNGA 7 cut(s) 163, 328, 451, 565, 655, 692, 766
Hpy8I GTNNAC 9 cut(s) 34, 121, 433, 739, 754, 1006, 1060, 1102, 1126
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 379, 652, 1021, 1130
HpyCH4III ACNGT 2 cut(s) 635, 736
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 3 cut(s) 698, 951, 1207
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 66, 1049
HpyF3I CTNAG 3 cut(s) 47, 543, 1046
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 9 cut(s) 322, 331, 376, 385, 418, 454, 568, 769, 1201
HspAI GCGC 1 cut(s) 672
KpnI GGTACC 1 cut(s) 634
Ksp22I TGATCA 1 cut(s) 618
KspAI GTTAAC 1 cut(s) 754
Kzo9I GATC 3 cut(s) 618, 688, 934
LmnI GCTCC 9 cut(s) 26, 156, 301, 409, 701, 804, 883, 925, 1185
Lsp1109I GCAGC 4 cut(s) 117, 653, 1168, 1249
LweI GCATC 3 cut(s) 685, 1264, 1276
MaeI CTAG 2 cut(s) 63, 1001
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 2 cut(s) 847, 854
MalI GATC 3 cut(s) 620, 690, 936
MboI GATC 3 cut(s) 618, 688, 934
MboII GAAGA 8 cut(s) 124, 200, 280, 670, 944, 952, 1237, 1300
MfeI CAATTG 2 cut(s) 1170, 1235
MhlI GDGCHC 2 cut(s) 922, 1045
MlsI TGGCCA 2 cut(s) 5, 323
MluCI AATT 3 cut(s) 581, 1170, 1235
MluNI TGGCCA 2 cut(s) 5, 323
MlyI GAGTC 2 cut(s) 48, 1239
Mox20I TGGCCA 2 cut(s) 5, 323
MscI TGGCCA 2 cut(s) 5, 323
MseI TTAA 2 cut(s) 53, 753
MslI CAYNNNNRTG 2 cut(s) 764, 1202
Msp20I TGGCCA 2 cut(s) 5, 323
MspI CCGG 6 cut(s) 153, 576, 804, 852, 1017, 1089
MspR9I CCNGG 3 cut(s) 154, 804, 1017
MunI CAATTG 2 cut(s) 1170, 1235
MvnI CGCG 1 cut(s) 611
MwoI GCNNNNNNNGC 3 cut(s) 57, 66, 1049
NciI CCSGG 3 cut(s) 154, 804, 1017
NdeII GATC 3 cut(s) 618, 688, 934
NlaIII CATG 9 cut(s) 322, 331, 376, 385, 418, 454, 568, 769, 1201
NlaIV GGNNCC 7 cut(s) 131, 474, 632, 721, 921, 1042, 1141
NmuCI GTSAC 1 cut(s) 847
NspI RCATGY 1 cut(s) 1201
PagI TCATGA 4 cut(s) 327, 450, 564, 765
PceI AGGCCT 1 cut(s) 222
PfeI GAWTC 1 cut(s) 552
Pfl23II CGTACG 1 cut(s) 366
PflFI GACNNNGTC 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 851
PkrI GCNGC 5 cut(s) 68, 107, 643, 1158, 1264
PleI GAGTC 2 cut(s) 47, 1238
PpsI GAGTC 2 cut(s) 47, 1238
Ppu21I YACGTR 1 cut(s) 366
PpuMI RGGWCCY 1 cut(s) 1139
Psp5II RGGWCCY 1 cut(s) 1139
PspLI CGTACG 1 cut(s) 366
PspN4I GGNNCC 7 cut(s) 131, 474, 632, 721, 921, 1042, 1141
PspPI GGNCC 2 cut(s) 1050, 1139
PspPPI RGGWCCY 1 cut(s) 1139
PstNI CAGNNNCTG 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 344
RsaI GTAC 5 cut(s) 368, 632, 812, 1190, 1250
RsaNI GTAC 5 cut(s) 367, 631, 811, 1189, 1249
RseI CAYNNNNRTG 2 cut(s) 764, 1202
SaqAI TTAA 2 cut(s) 53, 753
SatI GCNGC 5 cut(s) 67, 106, 642, 1157, 1263
Sau3AI GATC 3 cut(s) 618, 688, 934
Sau96I GGNCC 2 cut(s) 1050, 1139
ScaI AGTACT 1 cut(s) 1250
SchI GAGTC 2 cut(s) 48, 1239
ScrFI CCNGG 3 cut(s) 154, 804, 1017
SduI GDGCHC 2 cut(s) 922, 1045
SfaNI GCATC 3 cut(s) 685, 1264, 1276
SinI GGWCC 1 cut(s) 1139
SmiMI CAYNNNNRTG 2 cut(s) 764, 1202
SmlI CTYRAG 4 cut(s) 88, 124, 1184, 1252
SmoI CTYRAG 4 cut(s) 88, 124, 1184, 1252
Sse9I AATT 3 cut(s) 581, 1170, 1235
SseBI AGGCCT 1 cut(s) 222
SsiI CCGC 3 cut(s) 9, 66, 609
SspMI CTAG 2 cut(s) 63, 1001
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 152, 802, 1015
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 635, 736
TaiI ACGT 1 cut(s) 368
TaqI TCGA 3 cut(s) 183, 1029, 1152
TaqII GACCGA 1 cut(s) 215
TasI AATT 3 cut(s) 581, 1170, 1235
TatI WGTACW 2 cut(s) 810, 1248
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 552
Tru1I TTAA 2 cut(s) 53, 753
Tru9I TTAA 2 cut(s) 53, 753
TscAI CASTG 5 cut(s) 76, 306, 358, 530, 739
TseFI GTSAC 1 cut(s) 847
TseI GCWGC 4 cut(s) 105, 641, 1156, 1262
Tsp45I GTSAC 1 cut(s) 847
TspDTI ATGAA 7 cut(s) 201, 398, 403, 439, 670, 782, 1083
TspGWI ACGGA 3 cut(s) 324, 878, 1206
TspRI CASTG 5 cut(s) 76, 306, 358, 530, 739
Tth111I GACNNNGTC 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 1139
XceI RCATGY 1 cut(s) 1201
XspI CTAG 2 cut(s) 63, 1001
ZrmI AGTACT 1 cut(s) 1250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.