Rh1DG378400

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015136
Physical Location & Seq
Reverse (-)
60067457 .. 60068190
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG378400.1

Sequence Viewer

Length: 579 bp
ATGCTCGGAGACTTTGGGGCCAGAGACCCGTTCCCGGCAGAGATAGCCAGTGAGTTTGGAGACAAAGTACTGGGCTTCCAGAGCACAGAGCACAAGATCCTCATCCCAACTGTGTCTGCTTCCTCTCTAGCCCAGCAAGAGTGCACCCCTGTCTCTCCTCTCCAGGCTCCTATGCTTGAAGAGGACGCTCAACAGCTCCTCAGGAAGGTTCTTGGGTGGAGATTGATAGTTGGAGAAGATGGGCTCAAACTACAGTGCTTGTGGAAATTGAGAGATTACCAATGTGGGGTTGAACTCATCAATAGGGTTTATAAGGTTGTGGAAGCTGCAGGGCATTTCCCAAATCTTCACTTGGAGCAACCCAATCAAGTTAGAGCAGAGCTATGGACATCTTCCGTTGGTAAACTTCTTGCAAACTTTTACGACATGTTCTTGAGGCAGTTGCAAAACCATTTGTCAATGACAATACCAACTTCCATTGTTGCAGGAGGATTGACCATGAATGATTTCATTGTAGCTGCTAAGATAGATGAGATCAAAACATCGGATCTCGTGCCCGTGAGAAGAGCTTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

192

Amino Acids

21.43

Weight (kDa)

5.57

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 57 - 138 2.6e-13 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51110 AT5G51110
fragaria_vesca FvH4_7g26390
malus_domestica MD07G1239200.v1.1
prunus_persica Prupe.2G268000_v2.0.a1 Prupe.2G268000_v2.0.a1
pyrus_communis pycom07g21520
rosa_chinensis RchiOBHm_Chr1g0372941
rosa_laevigata RLG00000026864
rosa_multiflora Rmu_ssc0000425.1_g000015
rosa_roxburghii Rroxscaffold_4G00284590
rosa_rugosa Rorug01G0375100
rosa_samantha Rh1AG383600 Rh1BG347500 Rh1DG378400
rosa_wichuraiana Rw1G033800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
AclWI GGATC 2 cut(s) 91, 555
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 3 cut(s) 34, 205, 286
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 2 cut(s) 179, 293
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 196, 326, 382, 518, 569
AluI AGCT 5 cut(s) 196, 326, 382, 518, 569
Alw21I GWGCWC 3 cut(s) 86, 93, 146
Alw26I GTCTC 4 cut(s) 3, 18, 54, 157
Alw44I GTGCAC 1 cut(s) 142
AlwI GGATC 2 cut(s) 91, 555
AoxI GGCC 1 cut(s) 18
ApaLI GTGCAC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 326, 518
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 200
BaeGI GKGCMC 2 cut(s) 146, 558
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 551
Bbv12I GWGCWC 3 cut(s) 86, 93, 146
BbvI GCAGC 2 cut(s) 313, 505
BccI CCATC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 4 cut(s) 3, 18, 54, 157
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 2 cut(s) 251, 327
BisI GCNGC 2 cut(s) 327, 519
BlsI GCNGC 2 cut(s) 328, 520
BmcAI AGTACT 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 35, 164
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 19, 168
BmrFI CCNGG 2 cut(s) 35, 164
BmrI ACTGGG 1 cut(s) 80
BmuI ACTGGG 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 18
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 205, 286
Bse1I ACTGG 2 cut(s) 48, 75
Bse21I CCTNAGG 1 cut(s) 200
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 102
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 3 cut(s) 34, 205, 286
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 2 cut(s) 48, 75
BseRI GAGGAG 2 cut(s) 147, 188
BseSI GKGCMC 2 cut(s) 146, 558
BseXI GCAGC 2 cut(s) 313, 505
BseYI CCCAGC 1 cut(s) 132
BshFI GGCC 1 cut(s) 20
BsiHKAI GWGCWC 3 cut(s) 86, 93, 146
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 3 cut(s) 34, 205, 286
BsmAI GTCTC 4 cut(s) 3, 18, 54, 157
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 18
Bsp1286I GDGCHC 5 cut(s) 86, 93, 146, 246, 558
Bsp143I GATC 3 cut(s) 96, 534, 547
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 19, 168
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 2 cut(s) 91, 555
BspQI GCTCTTC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 18
BsrI ACTGG 2 cut(s) 48, 75
BssMI GATC 3 cut(s) 96, 534, 547
BssSI CACGAG 1 cut(s) 551
Bst2BI CACGAG 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 112, 255
Bst6I CTCTTC 2 cut(s) 174, 559
BstDEI CTNAG 3 cut(s) 200, 522, 576
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 102
BstKTI GATC 3 cut(s) 99, 537, 550
BstMAI GTCTC 4 cut(s) 3, 18, 54, 157
BstMBI GATC 3 cut(s) 96, 534, 547
BstMWI GCNNNNNNNGC 2 cut(s) 44, 81
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 33, 162
BstSFI CTRYAG 2 cut(s) 251, 327
BstSLI GKGCMC 2 cut(s) 146, 558
BstV1I GCAGC 2 cut(s) 313, 505
BstX2I RGATCY 2 cut(s) 96, 547
BstYI RGATCY 2 cut(s) 96, 547
Bsu36I CCTNAGG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 55, 260
Cfr13I GGNCC 1 cut(s) 18
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 68
CviAII CATG 2 cut(s) 427, 499
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 3 cut(s) 200, 522, 576
DpnI GATC 3 cut(s) 98, 536, 549
DpnII GATC 3 cut(s) 96, 534, 547
Eam1104I CTCTTC 2 cut(s) 174, 559
EarI CTCTTC 2 cut(s) 174, 559
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 18
Eco81I CCTNAGG 1 cut(s) 200
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 2 cut(s) 430, 502
FaiI YATR 5 cut(s) 173, 312, 385, 428, 500
FatI CATG 2 cut(s) 426, 498
Fnu4HI GCNGC 2 cut(s) 327, 519
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 2 cut(s) 327, 519
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 327, 519
GsaI CCCAGC 1 cut(s) 136
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 430, 502
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 2 cut(s) 144, 404
Hpy188I TCNGA 2 cut(s) 8, 547
Hpy188III TCNNGA 3 cut(s) 79, 202, 433
Hpy8I GTNNAC 2 cut(s) 144, 404
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 2 cut(s) 112, 255
HpyCH4V TGCA 5 cut(s) 144, 329, 413, 445, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 81
HpyF3I CTNAG 3 cut(s) 200, 522, 576
Hsp92II CATG 2 cut(s) 430, 502
Kzo9I GATC 3 cut(s) 96, 534, 547
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 3 cut(s) 172, 201, 355
Lsp1109I GCAGC 2 cut(s) 313, 505
MaeI CTAG 1 cut(s) 128
MalI GATC 3 cut(s) 98, 536, 549
MboI GATC 3 cut(s) 96, 534, 547
MboII GAAGA 5 cut(s) 191, 248, 338, 384, 576
MflI RGATCY 2 cut(s) 96, 547
MhlI GDGCHC 5 cut(s) 86, 93, 146, 246, 558
MluCI AATT 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 7 cut(s) 110, 133, 168, 175, 209, 429, 482
MroXI GAANNNNTTC 1 cut(s) 506
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 35, 164
MvaI CCWGG 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 44, 81
NciI CCSGG 1 cut(s) 35
NdeII GATC 3 cut(s) 96, 534, 547
NlaIII CATG 2 cut(s) 430, 502
NlaIV GGNNCC 2 cut(s) 19, 168
NspI RCATGY 1 cut(s) 430
PciI ACATGT 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 506
PkrI GCNGC 2 cut(s) 328, 520
PscI ACATGT 1 cut(s) 426
PsiI TTATAA 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 19, 168
PspPI GGNCC 1 cut(s) 18
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 2 cut(s) 96, 547
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 559
SatI GCNGC 2 cut(s) 327, 519
Sau3AI GATC 3 cut(s) 96, 534, 547
Sau96I GGNCC 1 cut(s) 18
ScaI AGTACT 1 cut(s) 69
ScrFI CCNGG 2 cut(s) 35, 164
SduI GDGCHC 5 cut(s) 86, 93, 146, 246, 558
SetI ASST 7 cut(s) 198, 210, 318, 328, 384, 520, 571
SfcI CTRYAG 2 cut(s) 251, 327
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 1 cut(s) 266
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 33, 162
TaaI ACNGT 2 cut(s) 112, 255
TasI AATT 1 cut(s) 266
TatI WGTACW 1 cut(s) 67
TscAI CASTG 2 cut(s) 55, 260
TseI GCWGC 2 cut(s) 326, 518
TspDTI ATGAA 2 cut(s) 499, 515
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 2 cut(s) 55, 260
VneI GTGCAC 1 cut(s) 142
XagI CCTNNNNNAGG 1 cut(s) 203
XceI RCATGY 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.