RchiOBHm_Chr2g0148721

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010612
Physical Location & Seq
Forward (+)
66483259 .. 66485092
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51825

Sequence Viewer

Length: 462 bp
ATGACATTTTCACCCCCAAACAAATTATTAAAGTACAAAATTCTCTCTCTCCTCCTACCGGACTCTATCTCAGACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACCTGCTTCTCCTCGTTGGAACGTCGGAGCCCAAGAATTCGATCCAGGGCGGTGAAGATTTCACTGCAGAAGTCGTCGCCTGGCAAGCCGATGACGGTGACGAAACCTTTCCGGCGGATTTTGGGACAACTCAGATCTGTTTCTCGATCTGAATCTTGGCCGGGTCTTCCTTGGGTAACCCGACGGCGACGAAGACTCAAGGTGGTTGAGGTTGAGGTCGGCAGAGGTGCGGTGGAGGAAGAAGCCACAGGAGACGAGCTCATTGGCCCAAAGCTTGAAGACCTGCCTGTCCGATATCGAGTTCAAGATTTGCCGGTCCGATTCGTCCCCAGTCATTCTGATCTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.25

Weight (kDa)

10.7

Isoelectric Point (pI)

77.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
AasI GACNNNNNNGTC 1 cut(s) 398
Acc36I ACCTGC 2 cut(s) 122, 402
AciI CCGC 3 cut(s) 161, 226, 341
AclWI GGATC 1 cut(s) 147
AcoI YGGCCR 1 cut(s) 269
AcsI RAATTY 2 cut(s) 39, 147
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 2 cut(s) 389, 416
AjnI CCWGG 2 cut(s) 155, 190
AluBI AGCT 2 cut(s) 370, 385
AluI AGCT 2 cut(s) 370, 385
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 1 cut(s) 147
AoxI GGCC 2 cut(s) 269, 376
ApoI RAATTY 2 cut(s) 39, 147
Asp700I GAANNNNTTC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 377, 427
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 4 cut(s) 3, 103, 175, 220
AvaII GGWCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 143, 372
BbsI GAAGAC 3 cut(s) 269, 310, 396
Bbv12I GWGCWC 1 cut(s) 372
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 2 cut(s) 157, 192
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 357
BfmI CTRYAG 1 cut(s) 176
BfuAI ACCTGC 2 cut(s) 122, 402
BglII AGATCT 1 cut(s) 245
Bme1390I CCNGG 3 cut(s) 157, 192, 273
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 377, 427
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 3 cut(s) 157, 192, 273
BmrI ACTGGG 1 cut(s) 435
BmuI ACTGGG 1 cut(s) 435
BpiI GAAGAC 3 cut(s) 269, 310, 396
BplI GAGNNNNNCTC 2 cut(s) 354, 386
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 156, 281
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 441
Bse8I GATNNNNATC 1 cut(s) 262
BseBI CCWGG 2 cut(s) 157, 192
BseDI CCNNGG 2 cut(s) 156, 281
BseJI GATNNNNATC 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 2 cut(s) 84, 256
BseNI ACTGG 1 cut(s) 441
BseRI GAGGAG 2 cut(s) 41, 112
BshFI GGCC 2 cut(s) 271, 378
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 4 cut(s) 59, 223, 272, 425
BslFI GGGAC 2 cut(s) 249, 422
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 249, 422
BsnI GGCC 2 cut(s) 271, 378
Bsp1286I GDGCHC 2 cut(s) 143, 372
Bsp143I GATC 4 cut(s) 152, 245, 257, 451
BspACI CCGC 3 cut(s) 161, 226, 341
BspANI GGCC 2 cut(s) 271, 378
BspCNI CTCAG 2 cut(s) 83, 255
BspLI GGNNCC 1 cut(s) 140
BspMAI CTGCAG 1 cut(s) 180
BspMI ACCTGC 2 cut(s) 122, 402
BspPI GGATC 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 441
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 2 cut(s) 156, 281
BssMI GATC 4 cut(s) 152, 245, 257, 451
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 157, 192
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 70, 242
BstEII GGTNACC 1 cut(s) 286
BstKTI GATC 4 cut(s) 155, 248, 260, 454
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 4 cut(s) 152, 245, 257, 451
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 2 cut(s) 157, 192
BstPI GGTNACC 1 cut(s) 286
BstSCI CCNGG 3 cut(s) 155, 190, 271
BstSFI CTRYAG 1 cut(s) 176
BstV2I GAAGAC 3 cut(s) 269, 310, 396
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 2 cut(s) 271, 378
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 2 cut(s) 122, 402
Cac8I GCNNGC 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 377, 427
CpoI CGGWCCG 1 cut(s) 427
Csp6I GTAC 1 cut(s) 34
CspI CGGWCCG 1 cut(s) 427
CviAII CATG 1 cut(s) 459
CviJI RGCY 7 cut(s) 141, 199, 271, 356, 370, 378, 385
CviKI_1 RGCY 7 cut(s) 141, 199, 271, 356, 370, 378, 385
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 70, 242
DpnI GATC 4 cut(s) 154, 247, 259, 453
DpnII GATC 4 cut(s) 152, 245, 257, 451
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 269
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 2 cut(s) 143, 372
Eco32I GATATC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 370
Eco91I GGTNACC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 155, 190
EcoRV GATATC 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 281
EcoT38I GRGCYC 2 cut(s) 143, 372
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 1 cut(s) 462
FaiI YATR 1 cut(s) 460
FaqI GGGAC 2 cut(s) 249, 422
FatI CATG 1 cut(s) 458
FriOI GRGCYC 2 cut(s) 143, 372
HaeIII GGCC 2 cut(s) 271, 378
HapII CCGG 4 cut(s) 59, 223, 272, 425
Hin1II CATG 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 5 cut(s) 62, 74, 263, 306, 432
HpaII CCGG 4 cut(s) 59, 223, 272, 425
HphI GGTGA 4 cut(s) 3, 103, 175, 220
Hpy188I TCNGA 7 cut(s) 73, 138, 245, 262, 404, 431, 451
Hpy188III TCNNGA 2 cut(s) 255, 416
Hpy99I CGWCG 4 cut(s) 138, 190, 297, 303
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 2 cut(s) 70, 242
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 1 cut(s) 462
Kzo9I GATC 4 cut(s) 152, 245, 257, 451
LmnI GCTCC 1 cut(s) 138
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 208, 286
MalI GATC 4 cut(s) 154, 247, 259, 453
MboI GATC 4 cut(s) 152, 245, 257, 451
MboII GAAGA 5 cut(s) 178, 269, 315, 362, 401
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 2 cut(s) 143, 372
MluCI AATT 3 cut(s) 23, 39, 147
MlyI GAGTC 3 cut(s) 56, 68, 300
MmeI TCCRAC 2 cut(s) 108, 116
MnlI CCTC 6 cut(s) 62, 133, 313, 319, 329, 340
MroXI GAANNNNTTC 1 cut(s) 218
MseI TTAA 1 cut(s) 29
MspI CCGG 4 cut(s) 59, 223, 272, 425
MspR9I CCNGG 3 cut(s) 157, 192, 273
MvaI CCWGG 2 cut(s) 157, 192
MwoI GCNNNNNNNGC 1 cut(s) 196
NciI CCSGG 1 cut(s) 273
NdeII GATC 4 cut(s) 152, 245, 257, 451
NlaIII CATG 1 cut(s) 462
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 208
PaqCI CACCTGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 263, 432
PleI GAGTC 3 cut(s) 56, 68, 300
PpsI GAGTC 3 cut(s) 56, 68, 300
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 155, 190
PspEI GGTNACC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 155, 190
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 377, 427
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
Rsr2I CGGWCCG 1 cut(s) 427
RsrII CGGWCCG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 1 cut(s) 29
Sau3AI GATC 4 cut(s) 152, 245, 257, 451
Sau96I GGNCC 2 cut(s) 377, 427
SchI GAGTC 3 cut(s) 56, 68, 300
ScrFI CCNGG 3 cut(s) 157, 192, 273
SduI GDGCHC 2 cut(s) 143, 372
SfcI CTRYAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 3 cut(s) 23, 39, 147
SsiI CCGC 3 cut(s) 161, 226, 341
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 3 cut(s) 155, 190, 271
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 136
TaqI TCGA 3 cut(s) 151, 256, 409
TasI AATT 3 cut(s) 23, 39, 147
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 2 cut(s) 263, 432
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 208
Tsp45I GTSAC 1 cut(s) 208
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 2 cut(s) 39, 147
XmnI GAANNNNTTC 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.