RchiOBHm_Chr2g0149621

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009066
Physical Location & Seq
Forward (+)
67452931 .. 67454415
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51902

Sequence Viewer

Length: 1194 bp
ATGGCTATCATCAATCACTTCTGCTCATGCCTACTACTTATCTTTCTCTTGTTCATGATATCCTTGTTCGGGTTATCGAATGCAGCCGCAACTTATAATGTGATGAAATATGGTGCAAAAGCAGATGGGAAAACAGACTCAACCCAAGCATTTGTCAAGACATGGGCAGCAGCATGCAGCTCTGCTCAGACAGCCACCATGTACGTCCCCAAAGGAAGTTTCTTGCTGAAAGCTGCAGTGTTCAGAGGCCCTTGCAAGAGTAGAATTATCGTTCAGATATACGGAACCCTGATTGCCCCTACTGATTATTGGGCTTTGGGAAACTCCGGTCACTGGATCTTGTTCATTAAGGTCAATAGACTTGAAGTTTTAGGTGGTAGACTCGACGCCAAGGGAGCTGGCTTTTGGGCTTGCAGGAAATCAGGAAAGAGCTGCCCTGTTGGAGCTAGGTCGATAACGTTCAATTGGGCAAATGACGTTGTGATAAGTGGTTTATCATCCGTCAACAGTCAGTTAACTCATCTTGTGATCAACAGCTGCAACAATGTGGCTGTCCGAAACGTTACGCTGTATGCTCCGGATGACAGCCCCAACACTGACGGCATTCATGTGCAGGGCTCAACTGGGGTTACTATCACTGGTGCAACCTTGATGACCGGAGACGATTGTGTATCAATTGGTCCTGGAACCAAAAACCTGTACATGAGTAACATCAGGTGCGGCCCAGGACATGGCGTAAGCATTGGTAGTCTAGGCAGGGCAGTGAACGAAGCTGGGGTCCAAAATGTGACGTTGACGAATGCAGTTTTCACCGGATCGGACAATGGAGTACGAATAAAGTCATGGGCGAGGCCCAGCACTGGGTTTGTGACCAACATTCTCTTCCAGAACATCATGATGAGGAACGTTGAGAACCCCATCATCATCAATCAGAACTATTGCCCACATAACCAAGGTTGCCCTAACCAGAGTTCTGGTGTGAAGATTAGTCAAGTAACATATAAGAATATACAAGGGACGTCGGCAACGGCGGAGGCGGTGACATTTGACTGCAGCCCAAGTAATCCGTGCAGAGGGATTAGATTGCATGACATCAAGCTTACATACCTAAATAAGGTAGCCACATCTTCATGCAATAACATTGGTGGAACCAGCACCGGAGTAGTCATGCCCAGGAGTTGCTTGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

397

Amino Acids

42.27

Weight (kDa)

9.33

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 33 - 95 1.6e-08 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 59 - 382 3e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 96
AatII GACGTC 1 cut(s) 1022
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 1 cut(s) 379
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 4 cut(s) 87, 720, 1031, 1037
AclI AACGTT 3 cut(s) 458, 561, 906
AclWI GGATC 2 cut(s) 344, 823
AcyI GRCGYC 2 cut(s) 387, 1019
AfaI GTAC 3 cut(s) 203, 701, 831
AfiI CCNNNNNNNGG 7 cut(s) 69, 333, 731, 860, 861, 1073, 1114
AgsI TTSAA 2 cut(s) 365, 463
AjnI CCWGG 3 cut(s) 682, 724, 1172
AjuI GAANNNNNNNTTGG 4 cut(s) 203, 235, 866, 898
AluBI AGCT 8 cut(s) 180, 233, 398, 432, 446, 537, 773, 1099
AluI AGCT 8 cut(s) 180, 233, 398, 432, 446, 537, 773, 1099
Alw26I GTCTC 1 cut(s) 654
AlwI GGATC 2 cut(s) 344, 823
Aor13HI TCCGGA 1 cut(s) 577
AoxI GGCC 3 cut(s) 247, 721, 851
ApeKI GCWGC 8 cut(s) 83, 167, 170, 177, 233, 432, 537, 1053
ArsI GACNNNNNNTTYG 2 cut(s) 762, 794
AspS9I GGNCC 5 cut(s) 248, 680, 722, 778, 852
AsuHPI GGTGA 2 cut(s) 802, 1051
AvaII GGWCC 2 cut(s) 680, 778
BanII GRGCYC 1 cut(s) 620
BbvI GCAGC 8 cut(s) 95, 179, 182, 189, 220, 419, 524, 1065
BccI CCATC 2 cut(s) 119, 926
BceAI ACGGC 2 cut(s) 616, 1044
BciT130I CCWGG 3 cut(s) 684, 726, 1174
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 654
BfaI CTAG 2 cut(s) 447, 752
BfmI CTRYAG 2 cut(s) 234, 1051
Bme1390I CCNGG 3 cut(s) 684, 726, 1174
Bme18I GGWCC 2 cut(s) 680, 778
BmgT120I GGNCC 5 cut(s) 248, 680, 722, 778, 852
BmiI GGNNCC 4 cut(s) 286, 688, 779, 1150
BmrFI CCNGG 3 cut(s) 684, 726, 1174
BmrI ACTGGG 2 cut(s) 633, 870
BmuI ACTGGG 2 cut(s) 633, 870
BsaHI GRCGYC 2 cut(s) 387, 1019
BsaJI CCNNGG 4 cut(s) 390, 724, 952, 1172
BsaWI WCCGGW 5 cut(s) 326, 577, 656, 812, 1157
Bsc4I CCNNNNNNNGG 7 cut(s) 69, 333, 731, 860, 861, 1073, 1114
Bse1I ACTGG 4 cut(s) 338, 628, 643, 865
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 3 cut(s) 684, 726, 1174
BseDI CCNNGG 4 cut(s) 390, 724, 952, 1172
BseGI GGATG 2 cut(s) 497, 586
BseLI CCNNNNNNNGG 7 cut(s) 69, 333, 731, 860, 861, 1073, 1114
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 4 cut(s) 338, 628, 643, 865
BseXI GCAGC 8 cut(s) 95, 179, 182, 189, 220, 419, 524, 1065
BseYI CCCAGC 2 cut(s) 773, 854
BsgI GTGCAG 2 cut(s) 632, 1090
BshFI GGCC 3 cut(s) 249, 723, 853
BsiSI CCGG 5 cut(s) 327, 578, 657, 813, 1158
BslFI GGGAC 2 cut(s) 191, 1030
BslI CCNNNNNNNGG 7 cut(s) 69, 333, 731, 860, 861, 1073, 1114
BsmAI GTCTC 1 cut(s) 654
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 2 cut(s) 191, 1030
BsmI GAATGC 3 cut(s) 85, 603, 805
BsnI GGCC 3 cut(s) 249, 723, 853
Bsp1286I GDGCHC 1 cut(s) 620
Bsp13I TCCGGA 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 3 cut(s) 336, 528, 815
BspACI CCGC 4 cut(s) 87, 720, 1031, 1037
BspANI GGCC 3 cut(s) 249, 723, 853
BspCNI CTCAG 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 577
BspHI TCATGA 2 cut(s) 54, 894
BspLI GGNNCC 4 cut(s) 286, 688, 779, 1150
BspMAI CTGCAG 2 cut(s) 238, 1055
BspPI GGATC 2 cut(s) 344, 823
BsrGI TGTACA 1 cut(s) 699
BsrI ACTGG 4 cut(s) 338, 628, 643, 865
BssECI CCNNGG 4 cut(s) 390, 724, 952, 1172
BssMI GATC 3 cut(s) 336, 528, 815
BssNI GRCGYC 2 cut(s) 387, 1019
BssT1I CCWWGG 2 cut(s) 390, 952
Bst2UI CCWGG 3 cut(s) 684, 726, 1174
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 887
BstACI GRCGYC 2 cut(s) 387, 1019
BstAUI TGTACA 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 175, 400, 412
BstDEI CTNAG 1 cut(s) 186
BstENI CCTNNNNNAGG 1 cut(s) 1112
BstF5I GGATG 2 cut(s) 497, 586
BstKTI GATC 3 cut(s) 339, 531, 818
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 3 cut(s) 336, 528, 815
BstMWI GCNNNNNNNGC 2 cut(s) 191, 395
BstNI CCWGG 3 cut(s) 684, 726, 1174
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 3 cut(s) 682, 724, 1172
BstSFI CTRYAG 2 cut(s) 234, 1051
BstV1I GCAGC 8 cut(s) 95, 179, 182, 189, 220, 419, 524, 1065
BstX2I RGATCY 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 974
BstYI RGATCY 1 cut(s) 336
BsuRI GGCC 3 cut(s) 249, 723, 853
BtsCI GGATG 2 cut(s) 497, 586
BtsI GCAGTG 2 cut(s) 243, 768
BtsIMutI CAGTG 6 cut(s) 243, 331, 594, 636, 768, 858
Cac8I GCNNGC 3 cut(s) 175, 400, 412
CciI TCATGA 2 cut(s) 54, 894
Cfr13I GGNCC 5 cut(s) 248, 680, 722, 778, 852
CseI GACGC 1 cut(s) 395
Csp6I GTAC 3 cut(s) 202, 700, 830
CviQI GTAC 3 cut(s) 202, 700, 830
DdeI CTNAG 1 cut(s) 186
DpnI GATC 3 cut(s) 338, 530, 817
DpnII GATC 3 cut(s) 336, 528, 815
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
EciI GGCGGA 1 cut(s) 1046
Eco130I CCWWGG 2 cut(s) 390, 952
Eco24I GRGCYC 1 cut(s) 620
Eco32I GATATC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 680, 778
EcoNI CCTNNNNNAGG 1 cut(s) 1112
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 3 cut(s) 682, 724, 1172
EcoRV GATATC 1 cut(s) 60
EcoT14I CCWWGG 2 cut(s) 390, 952
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 2 cut(s) 390, 952
Esp3I CGTCTC 1 cut(s) 654
FaqI GGGAC 2 cut(s) 191, 1030
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 379
FokI GGATG 2 cut(s) 484, 593
FriOI GRGCYC 1 cut(s) 620
FspBI CTAG 2 cut(s) 447, 752
GsaI CCCAGC 2 cut(s) 777, 858
HaeIII GGCC 3 cut(s) 249, 723, 853
HapII CCGG 5 cut(s) 327, 578, 657, 813, 1158
HgaI GACGC 1 cut(s) 395
Hin1I GRCGYC 2 cut(s) 387, 1019
HincII GTYRAC 3 cut(s) 505, 516, 795
HindII GTYRAC 3 cut(s) 505, 516, 795
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 2 cut(s) 137, 381
HpaI GTTAAC 1 cut(s) 516
HpaII CCGG 5 cut(s) 327, 578, 657, 813, 1158
HphI GGTGA 2 cut(s) 802, 1051
Hpy166II GTNNAC 5 cut(s) 380, 505, 516, 766, 795
Hpy188I TCNGA 6 cut(s) 189, 245, 276, 557, 820, 933
Hpy188III TCNNGA 6 cut(s) 55, 157, 423, 578, 886, 895
Hpy8I GTNNAC 5 cut(s) 380, 505, 516, 766, 795
Hpy99I CGWCG 2 cut(s) 389, 1024
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 7 cut(s) 204, 458, 477, 561, 791, 906, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 395
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 7 cut(s) 204, 458, 477, 561, 791, 906, 1019
Hsp92I GRCGYC 2 cut(s) 387, 1019
Kpn2I TCCGGA 1 cut(s) 577
Ksp22I TGATCA 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 516
Kzo9I GATC 3 cut(s) 336, 528, 815
LmnI GCTCC 3 cut(s) 395, 443, 580
Lsp1109I GCAGC 8 cut(s) 95, 179, 182, 189, 220, 419, 524, 1065
MaeI CTAG 2 cut(s) 447, 752
MaeII ACGT 7 cut(s) 204, 458, 477, 561, 791, 906, 1019
MaeIII GTNAC 8 cut(s) 329, 562, 628, 707, 787, 868, 994, 1039
MalI GATC 3 cut(s) 338, 530, 817
MboI GATC 3 cut(s) 336, 528, 815
MboII GAAGA 3 cut(s) 874, 994, 1119
MfeI CAATTG 2 cut(s) 463, 675
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 3 cut(s) 264, 463, 675
MlyI GAGTC 2 cut(s) 131, 375
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 5 cut(s) 239, 843, 894, 1027, 1067
MroI TCCGGA 1 cut(s) 577
MseI TTAA 2 cut(s) 348, 515
MslI CAYNNNNRTG 3 cut(s) 608, 896, 1129
MspA1I CMGCKG 1 cut(s) 537
MspI CCGG 5 cut(s) 327, 578, 657, 813, 1158
MspR9I CCNGG 3 cut(s) 684, 726, 1174
MunI CAATTG 2 cut(s) 463, 675
Mva1269I GAATGC 3 cut(s) 85, 603, 805
MvaI CCWGG 3 cut(s) 684, 726, 1174
MwoI GCNNNNNNNGC 2 cut(s) 191, 395
NdeII GATC 3 cut(s) 336, 528, 815
NlaIV GGNNCC 4 cut(s) 286, 688, 779, 1150
NmuCI GTSAC 4 cut(s) 329, 787, 868, 1039
NspI RCATGY 1 cut(s) 177
PaeI GCATGC 1 cut(s) 177
PagI TCATGA 2 cut(s) 54, 894
PctI GAATGC 3 cut(s) 85, 603, 805
PflMI CCANNNNNTGG 1 cut(s) 731
PfoI TCCNGGA 1 cut(s) 682
PleI GAGTC 2 cut(s) 131, 375
PpsI GAGTC 2 cut(s) 131, 375
PsiI TTATAA 1 cut(s) 96
Psp1406I AACGTT 3 cut(s) 458, 561, 906
Psp6I CCWGG 3 cut(s) 682, 724, 1172
PspFI CCCAGC 2 cut(s) 773, 854
PspGI CCWGG 3 cut(s) 682, 724, 1172
PspN4I GGNNCC 4 cut(s) 286, 688, 779, 1150
PspPI GGNCC 5 cut(s) 248, 680, 722, 778, 852
PstI CTGCAG 2 cut(s) 238, 1055
PsuI RGATCY 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 537
RsaI GTAC 3 cut(s) 203, 701, 831
RsaNI GTAC 3 cut(s) 202, 700, 830
RseI CAYNNNNRTG 3 cut(s) 608, 896, 1129
SaqAI TTAA 2 cut(s) 348, 515
Sau3AI GATC 3 cut(s) 336, 528, 815
Sau96I GGNCC 5 cut(s) 248, 680, 722, 778, 852
SchI GAGTC 2 cut(s) 131, 375
ScrFI CCNGG 3 cut(s) 684, 726, 1174
SduI GDGCHC 1 cut(s) 620
SfcI CTRYAG 2 cut(s) 234, 1051
SinI GGWCC 2 cut(s) 680, 778
SmiMI CAYNNNNRTG 3 cut(s) 608, 896, 1129
SmlI CTYRAG 1 cut(s) 1183
SmoI CTYRAG 1 cut(s) 1183
SphI GCATGC 1 cut(s) 177
Sse9I AATT 3 cut(s) 264, 463, 675
SsiI CCGC 4 cut(s) 87, 720, 1031, 1037
SspMI CTAG 2 cut(s) 447, 752
StyD4I CCNGG 3 cut(s) 682, 724, 1172
StyI CCWWGG 2 cut(s) 390, 952
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 7 cut(s) 207, 461, 480, 564, 794, 909, 1022
TaqI TCGA 3 cut(s) 77, 384, 452
TasI AATT 3 cut(s) 264, 463, 675
TatI WGTACW 1 cut(s) 699
TauI GCSGC 2 cut(s) 89, 723
Tru1I TTAA 2 cut(s) 348, 515
Tru9I TTAA 2 cut(s) 348, 515
TscAI CASTG 6 cut(s) 243, 338, 601, 643, 768, 865
TseFI GTSAC 4 cut(s) 329, 787, 868, 1039
TseI GCWGC 8 cut(s) 83, 167, 170, 177, 233, 432, 537, 1053
Tsp45I GTSAC 4 cut(s) 329, 787, 868, 1039
TspDTI ATGAA 5 cut(s) 43, 119, 334, 596, 1119
TspGWI ACGGA 3 cut(s) 297, 490, 1056
TspRI CASTG 6 cut(s) 243, 338, 601, 643, 768, 865
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 2 cut(s) 680, 778
XagI CCTNNNNNAGG 1 cut(s) 1112
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 379
XspI CTAG 2 cut(s) 447, 752
ZraI GACGTC 1 cut(s) 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.