Rw7G041010

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009066
Physical Location & Seq
Reverse (-)
63972648 .. 63974279
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041010.1

Sequence Viewer

Length: 1137 bp
ATGGCTATCATCAATCACTGCGCGTACCTATTACTTATCTCTCTCTTCTTCATAATATCCTTGTTCGGGTTATCCAATGCAGCCACAAATTATCATGTGATGAAATATGACACCCAAGCATTTGTCAAGACATGGGCAGCAGCATGCAGCTCTGCTCAGAAAGCCACCATGCATGTACCTGCAGTGTTCAGAGGCCCTTGCAAGAGTAGAATTAACGTTCAGATATACGGAACCTTGATTGCCCCGACTGATTATTGGGCTCTGGGAAACTCCGGCTATTGGATCTTATTTATTAAGGTCAATAGACTTGCATTTTTCGGTGGTACACTCGACGCCAAGGGAGCTGGCATTTGGGCTTGCAGGAAATCCGGAAAGAGCAGCCCTGTTGGAGCTAGGTCGATAACGTTCAATTGGGCAAATGACGTTGTGATTAGTGGTTTAGTATCCATCAACAGTCAGCAAACTCATCTTGTGATCAACAGCTGCAACAATGTGGTGTTCCGAAACGTAAAGCTGGTTGCTCCGGATGACAGCCCCAACACTGATGGCATTCGAGTGCAGATCTCAACTGGTGTTACTATCACTGGCGCAACCTTGCAAACCGGAGACGATTGTGAATCCAACCTCTACATGAGTAACATCAACATTGGTAGTCTAGGCAACGCAGTGAACGAAGCTGGGGTCCAAAATGTGACGTTGACGAATGCAATTTTCACTGGATCGGACAATGGAGTACGAATAAAGTCATGGGCCAGGCCCAGCAATAGTTTTGTGATTCTGGAAGAACATGATGATGAGGAACGAGAACCCCATCATCATCGATCAGAACTACTGCCCAAAAAAACGAAGATTTGTCAAGTGACATACAAGAATATACAAGGGACGTCGGCAAAGGCGGAGGCAGTAACATTTGATTGCAGCCCAAGTAATCCGTGCAGAGGGATTAGATTGCAAGACATCAAGCTTACATACATGAATCCGGTAGCCACATCTTCATGCAAGAACATTCGTGGAAGCACCACCGGAGTATACTCATGCCGGTACTCCGGTGGTACCACGGTTGATATTTTCCAATTCTTTGAAACCTTAATTAGTTCCCTTTTCCTTCATTTTCCAGGTTGGTTTTTGATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

378

Amino Acids

41.35

Weight (kDa)

8.7

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 49 - 251 4.3e-34 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 280 - 337 5.3e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 887
Acc36I ACCTGC 1 cut(s) 187
Acc65I GGTACC 1 cut(s) 1052
AccB1I GGYRCC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 1029
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 2 cut(s) 368, 523
AciI CCGC 1 cut(s) 896
AclI AACGTT 2 cut(s) 216, 404
AclWI GGATC 2 cut(s) 290, 727
AcyI GRCGYC 2 cut(s) 333, 884
AfaI GTAC 6 cut(s) 26, 177, 325, 735, 1043, 1054
AfiI CCNNNNNNNGG 3 cut(s) 66, 279, 938
AgsI TTSAA 2 cut(s) 409, 1082
AjnI CCWGG 2 cut(s) 752, 1114
AluBI AGCT 7 cut(s) 150, 344, 392, 483, 514, 677, 964
AluI AGCT 7 cut(s) 150, 344, 392, 483, 514, 677, 964
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 2 cut(s) 290, 727
Aor13HI TCCGGA 2 cut(s) 368, 523
AoxI GGCC 3 cut(s) 193, 750, 755
ApeKI GCWGC 7 cut(s) 80, 137, 140, 147, 378, 483, 918
ArsI GACNNNNNNTTYG 2 cut(s) 666, 698
Asp718I GGTACC 1 cut(s) 1052
AspLEI GCGC 2 cut(s) 23, 590
AspS9I GGNCC 4 cut(s) 194, 682, 750, 756
AvaII GGWCC 1 cut(s) 682
BaeI ACNNNNGTAYC 4 cut(s) 1033, 1044, 1066, 1077
BanI GGYRCC 1 cut(s) 1052
BanII GRGCYC 1 cut(s) 262
BbvI GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 930
BccI CCATC 3 cut(s) 455, 539, 819
BciT130I CCWGG 2 cut(s) 754, 1116
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 2 cut(s) 393, 656
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 187
BfuI GTATCC 1 cut(s) 454
BglII AGATCT 1 cut(s) 561
BisI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 919
BlsI GCNGC 7 cut(s) 82, 139, 142, 149, 380, 485, 920
Bme1390I CCNGG 2 cut(s) 754, 1116
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 4 cut(s) 194, 682, 750, 756
BmiI GGNNCC 3 cut(s) 232, 683, 1054
BmrFI CCNGG 2 cut(s) 754, 1116
Bsa29I ATCGAT 1 cut(s) 820
BsaHI GRCGYC 2 cut(s) 333, 884
BsaJI CCNNGG 2 cut(s) 336, 1056
BsaWI WCCGGW 6 cut(s) 368, 523, 602, 979, 1022, 1046
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 279, 938
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 3 cut(s) 574, 589, 721
BseAI TCCGGA 2 cut(s) 368, 523
BseBI CCWGG 2 cut(s) 754, 1116
BseCI ATCGAT 1 cut(s) 820
BseDI CCNNGG 2 cut(s) 336, 1056
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 3 cut(s) 66, 279, 938
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 3 cut(s) 574, 589, 721
BseXI GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 930
BseYI CCCAGC 2 cut(s) 677, 758
BsgI GTGCAG 2 cut(s) 578, 955
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 195, 752, 757
BshNI GGYRCC 1 cut(s) 1052
BshVI ATCGAT 1 cut(s) 820
BsiSI CCGG 8 cut(s) 273, 369, 524, 603, 980, 1023, 1039, 1047
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 3 cut(s) 66, 279, 938
BsmAI GTCTC 1 cut(s) 600
BsmBI CGTCTC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 895
BsmI GAATGC 2 cut(s) 549, 709
BsnI GGCC 3 cut(s) 195, 752, 757
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 2 cut(s) 368, 523
Bsp143I GATC 5 cut(s) 282, 474, 561, 719, 821
BspACI CCGC 1 cut(s) 896
BspANI GGCC 3 cut(s) 195, 752, 757
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 820
BspEI TCCGGA 2 cut(s) 368, 523
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 232, 683, 1054
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 290, 727
BspT107I GGYRCC 1 cut(s) 1052
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 3 cut(s) 574, 589, 721
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 2 cut(s) 336, 1056
BssMI GATC 5 cut(s) 282, 474, 561, 719, 821
BssNAI GTATAC 1 cut(s) 1030
BssNI GRCGYC 2 cut(s) 333, 884
BssT1I CCWWGG 1 cut(s) 336
Bst1107I GTATAC 1 cut(s) 1030
Bst2UI CCWGG 2 cut(s) 754, 1116
Bst4CI ACNGT 2 cut(s) 455, 1060
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 2 cut(s) 333, 884
BstC8I GCNNGC 3 cut(s) 145, 346, 358
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 1056
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 590
BstKTI GATC 5 cut(s) 285, 477, 564, 722, 824
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 5 cut(s) 282, 474, 561, 719, 821
BstMWI GCNNNNNNNGC 2 cut(s) 161, 341
BstNI CCWGG 2 cut(s) 754, 1116
BstNSI RCATGY 2 cut(s) 147, 176
BstSCI CCNGG 2 cut(s) 752, 1114
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 930
BstX2I RGATCY 2 cut(s) 282, 561
BstYI RGATCY 2 cut(s) 282, 561
BstZ17I GTATAC 1 cut(s) 1030
Bsu15I ATCGAT 1 cut(s) 820
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 3 cut(s) 195, 752, 757
BsuTUI ATCGAT 1 cut(s) 820
BtgI CCRYGG 1 cut(s) 1056
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 3 cut(s) 16, 189, 672
BtsIMutI CAGTG 6 cut(s) 16, 189, 540, 582, 672, 714
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 145, 346, 358
CfoI GCGC 2 cut(s) 23, 590
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 4 cut(s) 194, 682, 750, 756
ClaI ATCGAT 1 cut(s) 820
CseI GACGC 1 cut(s) 341
Csp6I GTAC 6 cut(s) 25, 176, 324, 734, 1042, 1053
CviQI GTAC 6 cut(s) 25, 176, 324, 734, 1042, 1053
DdeI CTNAG 1 cut(s) 156
DpnI GATC 5 cut(s) 284, 476, 563, 721, 823
DpnII GATC 5 cut(s) 282, 474, 561, 719, 821
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 911
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 752, 1114
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 600
FaqI GGGAC 1 cut(s) 895
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 1029
Fnu4HI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 919
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 919
FspBI CTAG 2 cut(s) 393, 656
GlaI GCGC 2 cut(s) 22, 589
GluI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 919
GsaI CCCAGC 2 cut(s) 681, 762
HaeIII GGCC 3 cut(s) 195, 752, 757
HapII CCGG 8 cut(s) 273, 369, 524, 603, 980, 1023, 1039, 1047
HgaI GACGC 1 cut(s) 341
HhaI GCGC 2 cut(s) 23, 590
Hin1I GRCGYC 2 cut(s) 333, 884
Hin6I GCGC 2 cut(s) 21, 588
HinP1I GCGC 2 cut(s) 21, 588
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 962
HinfI GANTC 3 cut(s) 617, 775, 976
HpaII CCGG 8 cut(s) 273, 369, 524, 603, 980, 1023, 1039, 1047
Hpy166II GTNNAC 4 cut(s) 326, 670, 699, 1030
Hpy188I TCNGA 6 cut(s) 159, 191, 222, 503, 724, 826
Hpy188III TCNNGA 4 cut(s) 127, 369, 524, 779
Hpy8I GTNNAC 4 cut(s) 326, 670, 699, 1030
Hpy99I CGWCG 2 cut(s) 335, 889
HpyAV CCTTC 1 cut(s) 1115
HpyCH4III ACNGT 2 cut(s) 455, 1060
HpyCH4IV ACGT 6 cut(s) 216, 404, 423, 507, 695, 884
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 341
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 6 cut(s) 216, 404, 423, 507, 695, 884
Hsp92I GRCGYC 2 cut(s) 333, 884
HspAI GCGC 2 cut(s) 21, 588
Kpn2I TCCGGA 2 cut(s) 368, 523
KpnI GGTACC 1 cut(s) 1056
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 5 cut(s) 282, 474, 561, 719, 821
LmnI GCTCC 3 cut(s) 341, 389, 526
Lsp1109I GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 930
MaeI CTAG 2 cut(s) 393, 656
MaeII ACGT 6 cut(s) 216, 404, 423, 507, 695, 884
MaeIII GTNAC 5 cut(s) 574, 635, 691, 859, 904
MalI GATC 5 cut(s) 284, 476, 563, 721, 823
MboI GATC 5 cut(s) 282, 474, 561, 719, 821
MboII GAAGA 5 cut(s) 37, 40, 794, 859, 984
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 2 cut(s) 282, 561
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 6 cut(s) 88, 210, 409, 708, 1073, 1089
MmeI TCCRAC 2 cut(s) 367, 645
MnlI CCTC 5 cut(s) 185, 635, 790, 892, 932
Mph1103I ATGCAT 1 cut(s) 174
MroI TCCGGA 2 cut(s) 368, 523
MseI TTAA 4 cut(s) 213, 294, 1088, 1135
MslI CAYNNNNRTG 3 cut(s) 554, 792, 994
MspA1I CMGCKG 1 cut(s) 483
MspI CCGG 8 cut(s) 273, 369, 524, 603, 980, 1023, 1039, 1047
MspR9I CCNGG 2 cut(s) 754, 1116
MunI CAATTG 1 cut(s) 409
Mva1269I GAATGC 2 cut(s) 549, 709
MvaI CCWGG 2 cut(s) 754, 1116
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 161, 341
NdeII GATC 5 cut(s) 282, 474, 561, 719, 821
NlaIV GGNNCC 3 cut(s) 232, 683, 1054
NmuCI GTSAC 2 cut(s) 691, 859
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 2 cut(s) 147, 176
PaeI GCATGC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 2 cut(s) 549, 709
PfeI GAWTC 3 cut(s) 617, 775, 976
PkrI GCNGC 7 cut(s) 82, 139, 142, 149, 380, 485, 920
Psp1406I AACGTT 2 cut(s) 216, 404
Psp6I CCWGG 2 cut(s) 752, 1114
PspFI CCCAGC 2 cut(s) 677, 758
PspGI CCWGG 2 cut(s) 752, 1114
PspN4I GGNNCC 3 cut(s) 232, 683, 1054
PspPI GGNCC 4 cut(s) 194, 682, 750, 756
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 282, 561
PvuII CAGCTG 1 cut(s) 483
RsaI GTAC 6 cut(s) 26, 177, 325, 735, 1043, 1054
RsaNI GTAC 6 cut(s) 25, 176, 324, 734, 1042, 1053
RseI CAYNNNNRTG 3 cut(s) 554, 792, 994
SaqAI TTAA 4 cut(s) 213, 294, 1088, 1135
SatI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 919
Sau3AI GATC 5 cut(s) 282, 474, 561, 719, 821
Sau96I GGNCC 4 cut(s) 194, 682, 750, 756
ScrFI CCNGG 2 cut(s) 754, 1116
SduI GDGCHC 1 cut(s) 262
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 682
SmiMI CAYNNNNRTG 3 cut(s) 554, 792, 994
SphI GCATGC 1 cut(s) 147
Sse9I AATT 6 cut(s) 88, 210, 409, 708, 1073, 1089
SsiI CCGC 1 cut(s) 896
SspMI CTAG 2 cut(s) 393, 656
StyD4I CCNGG 2 cut(s) 752, 1114
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 455, 1060
TaiI ACGT 6 cut(s) 219, 407, 426, 510, 698, 887
TaqI TCGA 4 cut(s) 330, 398, 553, 820
TasI AATT 6 cut(s) 88, 210, 409, 708, 1073, 1089
TfiI GAWTC 3 cut(s) 617, 775, 976
Tru1I TTAA 4 cut(s) 213, 294, 1088, 1135
Tru9I TTAA 4 cut(s) 213, 294, 1088, 1135
TscAI CASTG 6 cut(s) 23, 189, 547, 589, 672, 721
TseFI GTSAC 2 cut(s) 691, 859
TseI GCWGC 7 cut(s) 80, 137, 140, 147, 378, 483, 918
Tsp45I GTSAC 2 cut(s) 691, 859
TspDTI ATGAA 5 cut(s) 40, 116, 984, 989, 1097
TspGWI ACGGA 2 cut(s) 243, 921
TspRI CASTG 6 cut(s) 23, 189, 547, 589, 672, 721
VpaK11BI GGWCC 1 cut(s) 682
XceI RCATGY 2 cut(s) 147, 176
XmiI GTMKAC 1 cut(s) 1029
XspI CTAG 2 cut(s) 393, 656
ZraI GACGTC 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.